Influence of seed treatment with microbiological preparations and growth regulators on biometric characteristics of tomato plants

2021 ◽  
pp. 13-17
Author(s):  
Н.А. Жевнова ◽  
Е.А. Гырнец ◽  
А.А. Цыгичко ◽  
М.М. Астахов ◽  
Н.М. Сидоров

Цель работы: подбор регуляторов роста растений и биологических препаратов фунгицидного действия, оказывающих положительное влияние на энергию прорастания, всхожесть, рост и развитие растений томата сорта Транс Рио. Влияние регуляторов роста растений и микробиологических препаратов на энергию прорастания, всхожесть, рост и развитие растений было определено в лабораторных условиях на базе ФГБНУ ФНЦБЗР путем помещения обработанных семян во влажную камеру. Объектами исследования выступали регуляторы роста растений: Биодукс, Ж, ОберегЪ, Р, Иммуноцитофит, ТАБ; Рибав-Экстра, Р; биологические фунгициды на основе живых культур микроорганизмов: Гамаир, СП, Триходерма Вериде 471, СП, Псевдобактерин-2, Ж, Споробактерин, СП, а также штаммы В. velezensis BZR 517, В. velezensis BZR 336g. В работе был использован краситель Sapphire Seed Coat, разрешенный к применению в ЕС в органическом земледелии. Совместимость биопрепаратов была определена методом диффузии в агар. Исследование методом диффузии в арар показало, что краситель Sapphire Seed Coat не оказывает ингибирующего действия на микробиологические препараты Гамаир, СП; Триходерма 471, СП; Псевдобактерин-2, Ж; Споробактерин, СП и штаммы В. velezensis BZR 517, В. velezensis BZR 336g. Отмечено положительное влияние на рост и развитие томата препарата Гамаир, СП и штамма В. velezensis BZR 336g: увеличение массы корня на 23,5–52,9% и массы побега на 36,5–70,3%. Отмечено статистически значимое увеличение всех биометрических показателей при использовании смеси Иммуноцитофит, ТАБ и Sapphire Seed Coat: прибавка массы корня на 36,4%, длины и массы побега на 7,9 и 1,5% соответственно. Отобраны биопрепараты, которые будут задействованы в дальнейших этапах работы в качестве экологически безопасных средств для обработки семян томата с целью долгосрочного хранения (Гамаир, СП и штамм В. velezensisBZR 336g). Purpose of this work is to select plant growth regulators and biological preparations of fungicidal action that have a positive effect on the germination energy, germination, growth and development of tomato plants of the Trans Rio variety. The effect of plant growth regulators and microbiological preparations on germination energy, germination, growth and development of plants was determined in laboratory conditions at the base FSBSI FRCBPP by placing treated seeds in a humid chamber. The objects of the study were plant growth regulators: Biodux, F, Obereg, P, Immunocytophyte, TAB; Ribav-Extra, P; biological fungicides based on live cultures of microorganisms: Gamair, SP; Trichoderma Veride 471, SP, Pseudobacterin-2, F, Sporobacterin, SP, as well as strains of B. velezensisBZR 517, B. velezensis BZR 336g. The dye Sapphire Seed Coat, approved for use in the EU in organic farming, was used in the work. The compatibility of biological products was determined by the method of diffusion into agar. The method of diffusion into agar showed that the Sapphire Seed Coat does not have an inhibitory effect on the microbiological preparations Gamair, SP; Trichoderma 471, SP; Pseudobacterin-2, W; Sporobacterin, SP and the strains B. velezensis BZR 517, B. velezensis BZR 336g. A positive effect on the growth and development of tomato preparation Gamair, SP and strain B. velezensis BZR 336g was noted: an increase in root weight by 23.5–52.9% and shoot weight by 36.5–70.3%. There was a statistically significant increase in all biometric indicators when using a mixture of Immunocytophit, TAB and Sapphire Seed Coat: an increase in root weight by 36.4%, length and weight of the shoot by 7.9 and 1.5%, respectively. Biological products have been selected that will be used in the further stages of work as environmentally safe means for processing tomato seeds for long-term storage (Gamair, SP and B. velezensis strain BZR 336g).

2021 ◽  
Vol 13 (5) ◽  
pp. 2432
Author(s):  
Anna Makarova ◽  
Elena Nikulina ◽  
Tatiana Avdeenkova ◽  
Ksenia Pishaeva

Heavy metals are among the most widespread pollutants in soil. Phytoextraction technology is used to solve the problem of multi-metal-contaminated soil. The efficiency of this process can be increased by introducing various amendments. A soil amendment is any material added to a soil to improve its physical properties, such as water retention, permeability, water infiltration, drainage, aeration, and structure. Some chemical amendments for enhanced phytoextraction, such as amino polycarboxylates chelators, can be hazardous to the environment and perform poorly at pH > 8. The effect of the potassium salt of hydroxyethylidene diphosphonic acid (K2HEDP), plant growth regulators (PGRs), and iron chelate alone and in combination on the phytoextraction by Trifolium repens L. seedlings of Cd, Ni, and Cu was studied in this work. K2HEDP works in a wider pH range. The results of this study confirmed that amino polycarboxylate chelators, with the sodium salt of ethylene diamine tetraacetic acid (Na2EDTA) as an example, have a pronounced negative effect on the growth and development (organ mass) of Trifolium repens L. seedlings. K2HEDP, proposed by the authors instead of Na2EDTA, produced a pronounced positive effect on plant growth and development, which was further enhanced by the use of PGRs and with iron chelates. However, it should be noted that K2HEDP showed significantly lower efficiency in trials on the Trifolium repens L. seedlings. The highest was the efficiency of K2HEDP with PGRs and iron chelates for the phytoextraction of Cd.


2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Hammad Ishtiaq ◽  
Savita Bhardwaj ◽  
Aaliya Ashraf ◽  
Dhriti Kapoor

Plant growth regulators are significant chemical compounds which are synthesized inside the plant cells and play vital role in plant growth and development. Such compounds are usually active at very low concentrations. These plant growth regulators act as a signalling molecule, which influences the growth of plants. Throughout the previous year’s remarkable investigation have been done for understanding the synthesis of auxin and its effect on various physiological progressions. Auxin is a plant hormone that is involved in various physiological activities, including basic cellular processes such as cell enlargement, regulation of the cell cycle and distinction progress. Plants and several other microorganisms together produce auxin in order to carry out their cell cycle. The chemically synthesized auxins like NAA (naphthalene acetic acid) and IBA (Indole- butyric acid), also take part in various cellular processes. Against various types of biotic and abiotic stress conditions, these plant hormones significantly contribute in promoting acclimatization and adaptation in combination with other phytohormones. The present review highlights some of the important features of auxin role in regulation of plant growth either alone or in crosstalk with other plant hormones.


Author(s):  
V.S Muraviev ◽  
◽  
L.V. Dyaduchenko ◽  

We have carried out the synthesis and screening of soybean growth regulators in a series of substituted thieno[2,3-b]pyridines. The compounds, which have a high growth-regulating effect, were detected. According to the field tests, the substances have a positive effect in formation of the yield structure and provide seed quality.


2020 ◽  
pp. 54-57
Author(s):  
Ah. A. Suliman ◽  
A. G. Abramov ◽  
A. A. Shalamova

Relevance and methods. This study aimed to improve fruit set and plant performance to increase tomato productivity by studying the effect of plant growth regulators on tomato plants. A specific experiment has been carried out to study the effect of plant growth regulators Hemo bles active substances (850 g/kg) Humic Acid with applied doses (250, 500 and 700 ppm) and Magictone active substances (5-12.5 g/kg) naphthalene acetic acid and naphthalene acetamide with applied doses (250, 500 and 700 ppm) on growth and physiological characteristics of tomato plants (Big Beef F1). The experimental design was a Complete Randomized Blocks Design. Both Hemo bles and Magictone were applied three times (spraying on plants at 30 DAP, spraying on plants at 60 DAP and spraying on plants 90 DAP).Results. The obtained results showed that, Applying Humic Acid “Ener-850” had the highest significant fruit weight (137 g) during the two seasons. Also using “Magictone” had the highest significant Flowers number (48.1), Fruits Number (35.1), Flower Clusters number in the plant (13.6) and Fruits Number (54.6. while (Humic Acid) improved tomato fruit’s quality during improve Dry weight (75.1 g) of Arial parts, Ascorbic Acid, level of Vitamin C and Carotenoids contents (4.82 mg 100 g-1). The results were analyzed using one-way analysis of variance (ANOVA) followed by Tukey’s HSD test with α = 0.05 with the help of MINITAB (v. 19.0) program.


2020 ◽  
Vol 72 (3) ◽  
pp. 349-357
Author(s):  
Tatjana Cosic ◽  
Jelena Savic ◽  
Martin Raspor ◽  
Aleksandar Cingel ◽  
Nabil Ghalawnji ◽  
...  

Kohlrabi (Brassica oleracea var. gongylodes), with its edible stem tuber formed at the base of the plant stem, presents a valuable source of nutrients. The potential effects of plant growth regulators (PGRs), as well as various concentrations of different sugars on the in vitro development of kohlrabi were studied. Ten-day-old kohlrabi seedlings were cultivated in vitro for 5 weeks at 18?2?C on half-strength MS media containing different concentrations of carbon source such as sucrose, fructose, glucose, xylose and mannitol, combined with or without specific plant growth regulators (N6-benzyladenine (BA), gibberellic acid (GA3), 2,3,5-triiodobenzoic acid (TIBA)). Results showed no tuber formation in all treatments, but growth and development of treated kohlrabi seedlings was significantly affected in a distinctive manner, with a variety of morphological traits being altered in comparison to matching controls.


Author(s):  
Muhammad Imam Surya ◽  
Lily Ismaini ◽  
Suluh Normasiwi ◽  
Dwinda Mariska Putri ◽  
Vandra Kurniawan

Leaf is a key functional traits that shows respond of changes in plant physiology. This experiment aimed to study the changes on the leaf traits of loquat seedling that treated with plant growth regulators (PGRs). Three types of PGRs, auxin (naphthalene acetic acid/NAA), gibberellin (gibberellic acid/GA3) and cytokinin (benzylaminopurine/BA) with four doses (0, 25, 50, 100 ppm) were sprayed onto the leaves of loquat seedling. We observed nine parameters, PGRs treatments were significantly affecting eight parameters, while there were one parameter is not significantly affected. The results showed that either in mature or young leaves, PGRs treatments were significantly affecting in eight parameters the growth and development of leaves, such as leaf surface area, specific leaf area, fresh and dry weight leaf, water content, number of stomata, size of stomata, chlorophyll and transpiration rate compared to control. These results gave general view that PGRs treatment might stimulate leaf growth and development including photosynthesis and respiration. However, PGRs was not significantly affecting the number of stomata in young leaves. The application of PGRs doses was not always inline with the mean value of each parameters and it could be linear or quadratic models. The findings of this research could provide the recommendation for application of PGRs during seedling growth, and theoretical basis for comparison between mature and young leaves after PGRs application.


2020 ◽  
pp. 50-53
Author(s):  
Ah. A. Suliman ◽  
A. G. Abramov

Relevance. This study aimed to improve fruit set and plant performance to increase tomato productivity by studying the effect of plant growth regulators on tomato plants.Methods. A specific experiment has been carried out to study the effect of plant growth regulators Milagro (1% indol-3-butric acid) and Atlet active substances indol-3-butric acid and chloromequate chloride with applied doses (0.6 M/L, 1.0 M/L and 1.5 M/L) and (1.0, 1.5 and 2.0 M/L) on growth and physiological characteristics of plants (Big Beef F1). The experimental design was a Complete Randomized Blocks Design. Both Hemo bles was applied three times (spraying on plants at 30 DAP, spraying on plants at 60 DAP and spraying on plants 90 DAP).Results. The obtained results showed that, Applying Milagro (1% indol-3-butric acid) had the highest significant Plant height (80.13, 128.77 and 239 cm), number of leaves/plant (18.0, 34.67 and 44.3) and stem diameter (1.07, 1.5 and 2.03 cm), fruit weight (122.0 and 136 g), Flower Clusters number in the plant (4.64, 13.33 and 16.33) and Fruits Number (61.67, 62.0 and 67) Over the three years of study. The results were analyzed using one-way analysis of variance (ANOVA) followed by Tukey’s HSD test with α = 0.05 with the help of MINITAB (v. 19.0) program.


2020 ◽  
Vol 15 (1) ◽  
pp. 30-39
Author(s):  
Ahmad Ali Suliman ◽  
Alexandr Gennadevich Abramov ◽  
Anna Alekseevna Shalamova ◽  
Antar Mahmoud Badran

The study aimed to improve fruit set and plant performance to increase tomato productivity by studying the effect of plant growth regulators on tomato plants (Lycopersicon esculentum). A specific experiment has been carried out to study the effect of plant growth regulators Hemo bles (humic acid, 850g/kg) at applied doses (250, 500 and 700 ppm) and Magictone (naphthalene acetic acid and naphthalene acetamide, 5…12.5 g/kg) at applied doses (250, 500 and 700 ppm) on growth and physiological characteristics of tomato plants (Big Beef F1). The experimental design was a Complete Randomized Blocks Design. Both Hemo bles and Magictone were applied three times (spraying on plants at 30 days after planting (DAP), 60 DAP and 90 DAP). The obtained results showed that, applying Ener-850 humic acid caused the highest significant plant height (264.6 cm), number of leaves/plant (45), stem diameter (1.9 cm) and fruit weight (137 g) during the two seasons. In addition, applying Magictone resulted in the highest significant flower number (48.1), fruit number (35.1) and flower clusters number in the plant (13.6). Additionally, humic acid significantly increased dry weight (75.1 g) of arial parts with improving of tomato fruit quality via enhancing the concentrations of ascorbic acid, level of vitamin C and carotenoid content. The results were analyzed using one-way analysis of variance (ANOVA) followed by Tukey’s HSD test with α = 0.05 with the help of MINITAB (v. 19.0) program.


2020 ◽  
Vol 15 (6) ◽  
pp. 777-784
Author(s):  
A.A. Tedeeva ◽  
◽  
V.V. Tedeeva ◽  

The authors present the results of many years research on the study of the growth regulators “ХЭФ ВР” and Stabilan BP influence on the yield and quality of winter wheat of new varieties – Alekseich and Adel, selected by the National Center of Grain named after P. P. Lukyanenko in the steppe zone of the Mozdok region, Republic of North Ossetia – Alania. The aim of our research was to improve the tech nology of winter wheat cultivation using the new generation plant growth regulators “ХЭФК”, which stimulates the growth and volume of the root system, strengthens the stem by reducing the length of internodes and increasing the diameter of the stem, prevents lodging of crops, and has a positive effect on yield and quality. Stabilan slows down the growth of stem cells in length, which leads to a decrease in plant height and thickening of the spike, providing an increase in yield and grain quality. To achieve this goal, the research tasks included studying the effect of plant growth regulators on the growth processes of winter wheat at the initial stages of ontogenesis, on the yield and quality indicators of winter wheat grains of the Alekseich and Adel varieties in the steppe zone of the Mozdok region of the Republic of North Ossetia – Alania. Our studies have shown that growth regulators had a positive effect on the processing of seed material and sowing of winter wheat varieties Alekseich and Adel. The grain yield is increasing; the increase in relation to control was up to – 1.36 t/ha. The growth regulators “ХЭФК” and Stabilan were studied; they had a positive effect on the quality indicators of winter wheat grain, on the protein content, and the weight of 1000 grains. The highest yield was obtained on the variant with the use of the growth regulator “ХЭФК” – 4.63 t/ha.


Biomolecules ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 788
Author(s):  
Md. Quamruzzaman ◽  
S. M. Nuruzzaman Manik ◽  
Sergey Shabala ◽  
Meixue Zhou

Soil salinity is one of the major abiotic stresses restricting plant growth and development. Application of plant growth regulators (PGRs) is a possible practical means for minimizing salinity-induced yield losses, and can be used in addition to or as an alternative to crop breeding for enhancing salinity tolerance. The PGRs auxin, cytokinin, nitric oxide, brassinosteroid, gibberellin, salicylic acid, abscisic acid, jasmonate, and ethylene have been advocated for practical use to improve crop performance and yield under saline conditions. This review summarizes the current knowledge of the effectiveness of various PGRs in ameliorating the detrimental effects of salinity on plant growth and development, and elucidates the physiological and genetic mechanisms underlying this process by linking PGRs with their downstream targets and signal transduction pathways. It is shown that, while each of these PGRs possesses an ability to alter plant ionic and redox homeostasis, the complexity of interactions between various PGRs and their involvement in numerous signaling pathways makes it difficult to establish an unequivocal causal link between PGRs and their downstream effectors mediating plants’ adaptation to salinity. The beneficial effects of PGRs are also strongly dependent on genotype, the timing of application, and the concentration used. The action spectrum of PGRs is also strongly dependent on salinity levels. Taken together, this results in a rather narrow “window” in which the beneficial effects of PGR are observed, hence limiting their practical application (especially under field conditions). It is concluded that, in the light of the above complexity, and also in the context of the cost–benefit analysis, crop breeding for salinity tolerance remains a more reliable avenue for minimizing the impact of salinity on plant growth and yield. Further progress in the field requires more studies on the underlying cell-based mechanisms of interaction between PGRs and membrane transporters mediating plant ion homeostasis.


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