Improved sensitivity by optimizing the DNA extraction and PCR in molecular detection (16S) of bacteria from tissue

2016 ◽  
Author(s):  
Malin Bergman
Keyword(s):  
Dna Extraction ◽  
Molecular Detection

scholarly journals Molecular detection and occurrence of equine theileriosis in Arabian horses in Al-Najaf province/Iraq

2020 ◽  
Vol 57 (3) ◽  
pp. e166996
Author(s):  
Hayder Mohammad Al-Rammahi ◽  
Abdulameer Abed Hatem ◽  
Asaad Chasib Al-Atabi
Keyword(s):  
Polymerase Chain Reaction ◽  
Dna Extraction ◽  
Molecular Detection ◽  
Molecular Technique ◽  
Chain Reaction ◽  
Blood Samples ◽  
Equine Piroplasmosis ◽  
Polymerase Chain

This study was designed to detect equine piroplasmosis using the molecular technique in Al-Najaf province during the season that showed an increment in tick activities. Blood samples were collected from 110 horses with more than two signs of piroplasmosis. After DNA extraction, the product was examined by a polymerase chain reaction to amplify 18SrRNA. The results showed that the overall percentage of equine theileriosis was 38.18%. According to gender, the percentage of infection was 43.48% and 29.27% in females and males, respectively. Significant variations appeared between infected horses according to age, and the percentage of infection was 50% and 35.22% in less than 2 years and more than 2 years age, respectively. Moreover, the percentage of infection was 62.5% and 19.35% in animals with and without acariasis, respectively. Significant variations were also seen in equine theileriosis according to geographical areas, and the higher percentage was reported in Hera district (60.87%), while the lowest percentage was in the center of Al-Najaf (21.43%). This difference may be due to different distribution of vector of disease (tick), which may be the availability of the suitable weather that helped in the multiplication of the intermediate vectors. In conclusion, this study proved the variations in the occurrences of equine piroplasmosis according to gender, age, and geographical areas.

Rapid molecular detection ofEsteya vermicolabased on specific primers and the FTA-DNA extraction method

2014 ◽  
Vol 24 (8) ◽  
pp. 872-881 ◽  
Author(s):  
Ke Wei ◽  
Qinghua Wang ◽  
Yuzhu Wang ◽  
Liangjian Qu ◽  
Yong-an Zhang
Keyword(s):  
Dna Extraction ◽  
Extraction Method ◽  
Molecular Detection ◽  
Specific Primers ◽  
Dna Extraction Method

scholarly journals A Fast, Visual, and Instrument-free Platform Involving Rapid DNA Extraction, Chemical Heating, and Recombinase Aided Amplification for On-Site Nucleic Acid Detection

2021 ◽  
Vol 9 ◽  
Author(s):  
Xiao Fu Wang ◽  
Wen Qiang Chen ◽  
Jian Li Guo ◽  
Cheng Peng ◽  
Xiao Yun Chen ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Dna Extraction ◽  
Molecular Detection ◽  
Future Research ◽  
Nucleic Acid Detection ◽  
Valuable Insight ◽  
Detection Techniques ◽  
Detection Process ◽  
Novel Approach ◽  
Chemical Heating

The nucleic acid-based technique has been widely utilized in many fields including for on-site detection. However, traditional molecular detection techniques encounter limitations like relying on instruments, time consuming or complex operation, and cannot meet the demands of on-site testing. In this study, a rapid DNA extraction method (RDEM), recombinase aided amplification (RAA), and chemical heating packet (CHP) are integrated and termed as RRC platform for on-site detection of nucleic acid. For demonstration purposes, SHZD32-1 (a new transgenic soybean line from China) was detected using the novel platform to demonstrate its feasibility and capability for on-site detection. Using the RDEM, high-quality DNA appropriate for molecular detection was quickly extracted in 3–5 min. The heat energy generated by CHP was met the temperature requirements of RAA. Using the RRC platform, the whole detection process can be accomplished within only 30 min, and the results can be visually detected with glasses under blue light. No special or expensive instrument was needed for the detection process. This study provides a novel approach for on-site detection of nucleic acids besides providing valuable insight on related future research.

Molecular Detection of Neospora caninum from Naturally Infected Dogs in Van Province, East Turkey

2020 ◽  
Author(s):  
Ali Bilgin Yilmaz ◽  
Yaşar Göz ◽  
Özlem Orunç Kilinç ◽  
Vural Denizhan
Keyword(s):  
Polymerase Chain Reaction ◽  
Dna Extraction ◽  
Neurological Disorders ◽  
Genomic Dna ◽  
Molecular Detection ◽  
Neospora Caninum ◽  
Specific Method ◽  
Coccidian Parasite ◽  
Chain Reaction ◽  
Polymerase Chain

Neospora caninum is a coccidian parasite causing abortion in cattle and neurological problems in horses. Dogs are definitive hosts of N. caninum. Polymerase chain reaction is the most specific method used for the detection of N. caninum oocytes. In the present study, a total of 100 fecal samples were collected from naturally infected dogs. Of the 100 samples analyzed, 11 of them were detected with Hammondia/Neospora-like oocytes. Genomic DNA was isolated using a commercially available DNA extraction kit. The Nc5 gene specific to N. caninum was amplified by PCR and two of the eleven samples with Hammondia/Neospora-like oocytes formed ~337 bp repeatable band. In conclusion, N. caninum, which has been shown to cause neurological disorders in dogs and to abortion in cattle, was detected in naturally infected dogs in Van Province in Polymerase chain reaction.

scholarly journals First molecular detection of Eimeria spp. in eared doves (Zenaida auriculata) from Brazil

2020 ◽  
Vol 41 (4) ◽  
pp. 1259
Author(s):  
Alessandra Taroda ◽  
Luiz Daniel de Barros ◽  
Mércia de Seixas ◽  
Sérgio Tosi Cardim ◽  
João Pedro Sasse ◽  
...  
Keyword(s):  
Cytochrome C ◽  
Mitochondrial Genome ◽  
Cytochrome C Oxidase ◽  
Dna Extraction ◽  
Nested Pcr ◽  
Molecular Detection ◽  
Southern Brazil ◽  
Oxidase Gene ◽  
Fecal Samples ◽  
Eimeria Spp

The aim of the present study was to detect Eimeria spp. in eared doves (Zenaida auriculata) from Brazil. Two hundred and fourteen birds were trap-capture in different regions of Londrina city, Paraná state, Southern Brazil. Fecal samples were collected, and DNA extraction was performed. A nested PCR based on the subunit I of the cytochrome c oxidase gene of the Eimeria mitochondrial genome was used to detect the DNA presence of this coccidian in eared dove feces. From 214 birds, 171 (79.9%) were positive for Eimeria spp. There was significantly difference of positivity between the site of capture. When analyzing the genders, the numbers of positive males were 84/103 (81.5%) and females 87/111 (78.4%). To the best of the authors’ knowledge, this is the first study with molecular prevalence of Eimeria sp. in Z. auriculata. Further studies should be done to identify the species of Eimeria that infect eared doves Z. auriculata.

scholarly journals Enhanced rapid commercial DNA extraction kit for the molecular detection of severe acute respiratory syndrome coronavirus 2: Easy adaptation to current protocols

2021 ◽  
Vol 54 ◽  
Author(s):  
Rômulo Pessoa-e-Silva ◽  
Priscilla Stela Santana de Oliveira ◽  
Sayonara Maria Calado Gonçalves ◽  
Klarissa Miranda Guarines ◽  
Lidiane Vasconcelos do Nascimento Carvalho ◽  
...  
Keyword(s):  
Severe Acute Respiratory Syndrome ◽  
Dna Extraction ◽  
Molecular Detection

scholarly journals Molecular Detection of Mycoplasma haemofelis and `Candidatus Mycoplasma haemominutum' Infection in Cats by Direct PCR Using Whole Blood without DNA Extraction

2008 ◽  
Vol 70 (10) ◽  
pp. 1095-1099 ◽  
Author(s):  
Masashi WATANABE ◽  
Masaharu HISASUE ◽  
Takehisa SOUMA ◽  
Shuichi OHSHIRO ◽  
Takatsugu YAMADA ◽  
...  
Keyword(s):  
Dna Extraction ◽  
Whole Blood ◽  
Molecular Detection ◽  
Direct Pcr
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