scholarly journals KARAKTERISTIK DASAR POPULASI BAKTERI PENAMBAT NITROGEN NON-SIMBIOTIK PADA LAHAN GAMBUT BEKAS KEBAKARAN

2019 ◽  
Vol 7 (3) ◽  
Author(s):  
Menik Desmina ◽  
Wiwik Ekyastuti
Keyword(s):  
Population Density ◽  
Plant Growth ◽  
Bacterial Population ◽  
Soil Sampling ◽  
Systematic Sampling ◽  
Nitrogen Fixing ◽  
Nitrogen Fixing Bacteria ◽  
Plate Method ◽  
Yeast Extract Mannitol ◽  
Basic Characteristics

Nitrogen is an macroessential nutrient for plant growth. Insufficient nitrogen or nitrogen elements, the plant will be upnormal. The availability of nitrogen can be obtained through one of the microbes in the soil, namely nitrogen fixing bacteria (NFB) that is capable of fixing free nitrogen, including symbiotic and non-symbiotic. This study aimd to describe the density of population and the basic characteristics of NFB in burnt peatland Kuala Dua village. The method was in soil sampling with systematic sampling. Bacteria isolation using yeast extract mannitol agar (YEMA) media by using the pour plate method. The density of bacterial population was calculated by count pour method. The result showed tha the higest population density in peat from at distance of 80 m from the edge of the trench with a depth of 0-20 cm as much (52.107cfu/g soil), while a low population density was found at a distance of 20 m from the edge of a trench with a depth of 20-50 cm by much (15,5.107cfu/g soil). The bacterial population in burnt peatland fall was categorited the high. there potential can developed to support the restoration of peatland ecosystems originating from burnt peatlands.Keywords: charakteristics, burnt peatlands, nitrogen fixing bacteria, restoration.

scholarly journals Genomic characterization and computational phenotyping of nitrogen-fixing bacteria isolated from Colombian sugarcane fields

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Luz K. Medina-Cordoba ◽  
Aroon T. Chande ◽  
Lavanya Rishishwar ◽  
Leonard W. Mayer ◽  
Lina C. Valderrama-Aguirre ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Nitrogen Fixation ◽  
Plant Growth ◽  
Phosphate Solubilization ◽  
Nitrogen Fixing ◽  
Nitrogen Fixing Bacteria ◽  
Promising Tool ◽  
A Genome ◽  
Plant Growth Promoting ◽  
Growth Promoting

AbstractPrevious studies have shown the sugarcane microbiome harbors diverse plant growth promoting microorganisms, including nitrogen-fixing bacteria (diazotrophs), which can serve as biofertilizers. The genomes of 22 diazotrophs from Colombian sugarcane fields were sequenced to investigate potential biofertilizers. A genome-enabled computational phenotyping approach was developed to prioritize sugarcane associated diazotrophs according to their potential as biofertilizers. This method selects isolates that have potential for nitrogen fixation and other plant growth promoting (PGP) phenotypes while showing low risk for virulence and antibiotic resistance. Intact nitrogenase (nif) genes and operons were found in 18 of the isolates. Isolates also encode phosphate solubilization and siderophore production operons, and other PGP genes. The majority of sugarcane isolates showed uniformly low predicted virulence and antibiotic resistance compared to clinical isolates. Six strains with the highest overall genotype scores were experimentally evaluated for nitrogen fixation, phosphate solubilization, and the production of siderophores, gibberellic acid, and indole acetic acid. Results from the biochemical assays were consistent and validated computational phenotype predictions. A genotypic and phenotypic threshold was observed that separated strains by their potential for PGP versus predicted pathogenicity. Our results indicate that computational phenotyping is a promising tool for the assessment of bacteria detected in agricultural ecosystems.

Nitrogen-fixing bacteria with multiple plant growth-promoting activities enhance growth of tomato and red pepper

2013 ◽  
Vol 53 (12) ◽  
pp. 1004-1015 ◽  
Author(s):  
Md. Rashedul Islam ◽  
Tahera Sultana ◽  
M. Melvin Joe ◽  
Woojong Yim ◽  
Jang-Cheon Cho ◽  
...  
Keyword(s):  
Plant Growth ◽  
Red Pepper ◽  
Nitrogen Fixing ◽  
Nitrogen Fixing Bacteria ◽  
Plant Growth Promoting Activities ◽  
Plant Growth Promoting ◽  
Growth Promoting

scholarly journals Potassium solubilization in phonolite rock by diazotrophic bacteria

2017 ◽  
Vol 8 (1) ◽  
pp. 17 ◽  
Author(s):  
Ligiane Aparecida Florentino ◽  
Adauton Vilela Rezende ◽  
Cássia Cristina Bachião Miranda ◽  
Aline Carvalho Mesquita ◽  
José Ricardo Mantovani ◽  
...  
Keyword(s):  
Plant Growth ◽  
Ph Value ◽  
Carbon Sources ◽  
Control Treatment ◽  
Nitrogen Fixing ◽  
Diazotrophic Bacteria ◽  
Bacterial Strains ◽  
Nitrogen Fixing Bacteria ◽  
Rock Powder ◽  
Potential Use

Some strains of nitrogen fixing bacteria, besides fixing the N2, can produce phytohormones, control pathogens, act in solubilization of minerals containing phosphorus (P) and potassium (K), contributing to plant growth. However, the solubilization of minerals containing K has been little exploited. Therefore, the aim of this study was to evaluate potassium solubilization (K) of phonolite rock by strains of associative nitrogen fixing bacteria supplied with two different carbon sources. Twelve bacterial strains were cultured for seven days at 25°C in Aleksandrov medium supplemented with phonolite rock powder. The experiment was carried out in a completely randomized factorial design, 13 x 2 (12 bacterial strains and a control without inoculation) and two carbon sources (glucose and sucrose) with four repetitions. After the growth, the supernatant was separated by centrifugation and analyzed for the final pH value and the content of K. All diazotrophic bacteria strains contributed to increase the release of K when compared to the control treatment. The strain UNIFENAS 100-94 solubilized 130 mg L-1 K in the presence of the two carbon sources, indicating the potential use of these diazotrophic bacterial strains for K solubilization from minerals.

scholarly journals Rhizosphere Microbiome Modulators: Contributions of Nitrogen Fixing Bacteria towards Sustainable Agriculture

2018 ◽  
Author(s):  
Nicholas Ozede Igiehon ◽  
Olubukula Oluranti Babalola
Keyword(s):  
Plant Growth ◽  
Sustainable Agriculture ◽  
Agricultural Development ◽  
Agricultural Land ◽  
Anthropogenic Activities ◽  
Belowground Biomass ◽  
Growth And Yield ◽  
Anthropogenic Activity ◽  
Nitrogen Fixing ◽  
Nitrogen Fixing Bacteria

Rhizosphere microbiomes which have been implicated to enhance plant growth and yield are modulated or influenced by a couple of environmental factors such as soil type, plant cultivar, climate change and anthropogenic activities. In particular, anthropogenic activity such as the use of nitrogen-based chemical fertilizers is associated with environmental destruction and this call for a more ecofriendly strategy to increase nitrogen level of agricultural land. This feat is attainable by harnessing nitrogen-fixing endophytic and free-living rhizobacteria. Rhizobium, Pseudomonas, Azospirillum and Bacillus have been found to have positive impacts on crops by enhancing both above and belowground biomass and could therefore play positive roles in achieving sustainable agriculture. Thus, it is needful to study these rhizosphere microbiomes with more sophisticated culture-independent technologies such as next generation sequencing (NGS) with the prospect of discovering novel bacteria with plant growth promoting traits. This review is therefore aimed at discussing factors that can modulate rhizosphere microbiomes with focus on the contributions of nitrogen fixing bacteria towards sustainable agricultural development and the techniques that can be used for their study.

scholarly journals Diversity of non-symbiotic nitrogen-fixing bacteria and their potential in andisols affected by the eruption of Mount Sinabung, North Sumatra, Indonesia

2021 ◽  
Vol 22 (8) ◽  
Author(s):  
Mariani Sembiring ◽  
T. SABRINA
Keyword(s):  
Plant Growth ◽  
Soil Fertility ◽  
Enterobacter Cloacae ◽  
Soil Samples ◽  
Biological Characteristics ◽  
Soil N ◽  
Nitrogen Fixing ◽  
Nitrogen Fixing Bacteria ◽  
Beneficial Microbes ◽  
North Sumatra

Abstract. Sembiribg M, Sabrina T. 2021. Diversity of non-symbiotic nitrogen-fixing bacteria and their potential in andisols affected by the eruption of Mount Sinabung, North Sumatra, Indonesia. Biodiversitas 22: 3539-3544. Nitrogen is the main macro-nutrient that is very important for plant growth. Nitrogen is absorbed by plants in the form of NO3-or NH4+ ions from the soil. The andisols affected by the eruption of Mount Sinabung alter the chemical, physical and biological characteristics of the soil. As a result, the population of beneficial microbes in the soil decreased, so soil fertility also decreased. The aim of this research is to determine the diversity of nitrogen-fixing microbes in andisol soil affected by the Mt. Sinabung eruption. The soil samples used in this research were collected from andisols affected by the eruption of Mount Sinabung. The isolation of non-symbiotic nitrogen-fixing bacteria was carried out using a nitrogen-free Jensen medium. The results showed that five non symbiotic N-fixing bacteria can increase the N content in andisols affected by Mount Sinabung eruption. Enterobacter cloacae can increase soil N by 111.76% as compared to without microbial application.

scholarly journals PARTIAL STUDY OF NON SYMBOTIC NITROGEN FIXING BACTERIA FROM RHIZOSFER OF GAMAL AS PLANT GROWTH PROMOITING ON THE LAND OF THREE STRATA SYSTEMS

Pastura ◽  
2019 ◽  
Vol 7 (2) ◽  
pp. 78
Author(s):  
N.G.K. Roni ◽  
S.A. Lindawati
Keyword(s):  
Plant Growth ◽  
Water Content ◽  
Plate Count ◽  
Soil Conditions ◽  
Nitrogen Fixing ◽  
Nitrogen Fixing Bacteria ◽  
Compact Structure ◽  
Dry Land ◽  
Total Plate Count ◽  
Fertile Land

Gamal is one of the tropical forages that can still grow and produce f on dry land in the hot season, while other plants, especially the grass has dried so it needs to be examined soil conditions in the rhizosphere. A study aimed at studying soil conditions in rhizosphere of gamal plants and obtaining isolates of non symbiotic nitrogen-fixing bacteria from gamal rhizosphere that function as Plant Growth Promoting on three strata land (STS) Pecatu, Bukit jimbaran, Badung, Bali. The research begins with observation of soil texture and color, soil structure determination and water content, pH, and soil total plate count, followed by calculation of non-symbiotic N fixing bacteria,, isolation, gram staining test, fermnetatif test, and catalase test. The results showed that soil characteristics of STS Pecatu land include clay clay texture, compact structure, brownish color, pH 6.79-7.40, water content 14,19-18,12, STS Pecatu land including fertile land with total plate count 1.65x 107 cfu/g, and 6 non-symbiotic non-beneficial bacterial inhibitors were suspected from the genus Azotobacter sp, with colonies of 83 x 105 cfu/g. Based on the result of the research, it can be concluded that the soil of STS Pecatu land including fertile land with total plate count 16,5 x 106 cfu/g, and found 6 isolate of non-symbiotic N fixing bacteria from genus Azotobacter sp, with number of colony 83 x 105 cfu/g. Keywords: Non-symbiotic N-fixing bacteria, Three-Strata System, Pecatu

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