scholarly journals An expanded palette of fluorogenic HaloTag probes with enhanced contrast for targeted cellular imaging

2021 ◽  
Author(s):  
Sylvestre Bachollet ◽  
Yuriy Shpinov ◽  
Fanny Broch ◽  
Hela Benaissa ◽  
Arnaud Gautier ◽  
...  
Keyword(s):  
Hela Cells ◽  
Cellular Imaging ◽  
Targeted Imaging ◽  
Molecular Rotor ◽  
Specific Signal ◽  
Rotor Design ◽  
Intracellular Organelles ◽  
Good Contrast

We report on new fluorogenic HaloTag probes based on a molecular rotor design. Thanks to their viscosity-sensitive emission, the probes light-up upon reaction with the protein self-labeling tag HaloTag. The palette of probes cover an emission range from green to red and exhibit remarkably low non-specific signal that enabled wash-free targeted imaging of intracellular organelles and proteins with good contrast in live Hela cells.

Fabrication of ZnO nanoplates for visible light-induced imaging of living cells

2014 ◽  
Vol 2 (16) ◽  
pp. 2311-2317 ◽  
Author(s):  
Jooran Lee ◽  
Joon Sig Choi ◽  
Minjoong Yoon
Keyword(s):  
Visible Light ◽  
Hela Cells ◽  
Fluorescence Emission ◽  
Green Emission ◽  
Living Cells ◽  
Cellular Imaging ◽  
Red Fluorescence ◽  
Light Excitation

APTES-modified ZnO nanoplates (NPls) showed excellent permeability into HeLa cells with negligible cytotoxicity, exhibiting strong red fluorescence emission (∼650 nm) under visible light excitation at 405 nm. Therefore, the synthesized ZnO NPls would be useful for highly resolved cellular imaging by avoiding the overlap with the cellular intrinsic green emission.

Terpyridine Oxovanadium(IV) Complexes of Phenanthroline Bases for Cellular Imaging and Photocytotoxicity in HeLa Cells

2011 ◽  
Vol 2011 (9) ◽  
pp. 1425-1435 ◽  
Author(s):  
Bhabatosh Banik ◽  
Pijus K. Sasmal ◽  
Sovan Roy ◽  
Ritankar Majumdar ◽  
Rajan R. Dighe ◽  
...  
Keyword(s):  
Hela Cells ◽  
Cellular Imaging ◽  
Phenanthroline Bases

Transferrin-directed preparation of red-emitting copper nanoclusters for targeted imaging of transferrin receptor over-expressed cancer cells

2015 ◽  
Vol 3 (11) ◽  
pp. 2388-2394 ◽  
Author(s):  
Tong Zhao ◽  
Xi-Wen He ◽  
Wen-You Li ◽  
Yu-Kui Zhang
Keyword(s):  
Cancer Cells ◽  
Hela Cells ◽  
Transferrin Receptor ◽  
Targeted Imaging ◽  
Copper Nanoclusters

Red-emitting fluorescent transferrin-functionalized copper nanoclusters were synthesized for the targeted bioimaging of HeLa cells.

Enzyme-responsive fluorescent camptothecin prodrug/polysaccharide supramolecular assembly for targeted cellular imaging and in situ controlled drug release

2020 ◽  
Vol 56 (7) ◽  
pp. 1042-1045 ◽  
Author(s):  
Yu-Hui Zhang ◽  
Ying-Ming Zhang ◽  
Xianliang Sheng ◽  
Jie Wang ◽  
Yu Liu
Keyword(s):  
Drug Delivery ◽  
Drug Release ◽  
Real Time ◽  
Supramolecular Assembly ◽  
Controlled Drug Release ◽  
Cellular Imaging ◽  
Targeted Imaging ◽  
Real Time Tracking ◽  
Low Cytotoxicity

An enzyme-responsive polysaccharide assembly was constructed, which possesses low cytotoxicity, targeted imaging and controlled drug release, while providing a concurrent means for the real-time tracking of drug delivery.

Bulky 4,6-disubstituted tetraphenylethene–naphthalimide dyad: synthesis, copolymerization, stimuli-responsive fluorescence and cellular imaging

2017 ◽  
Vol 196 ◽  
pp. 439-454 ◽  
Author(s):  
Qiong-Xin Hua ◽  
Bo Xin ◽  
Jun-Xia Liu ◽  
Ling-Xi Zhao ◽  
Zu-Jing Xiong ◽  
...  
Keyword(s):  
Fluorescence Intensity ◽  
Hela Cells ◽  
Thermal Effects ◽  
Uv Light ◽  
Targeted Imaging ◽  
Stimuli Responsive ◽  
Design And Synthesis ◽  
Acrylate Copolymer ◽  
Fluorescence Change ◽  
Vinyl Group

We report the design and synthesis of a tetraphenylethene substituted with naphthalimide at the 4, 6 positions, named NI-2TPE. NI-2TPE exhibits strong solvent-dependent emission properties with combined ICT and AIE characteristics in THF–H2O systems. This probe was used directly on test papers to distinguish normal organic solvents using their emission colours under UV light based on its AIE and ICT nature. Thanks to the vinyl group in NI-2TPE, we synthesized a copolymer of NIPAM and NI-2TPE, termed P(NIPAM-co-NI-2TPE). The resulting polymer is highly soluble and fluorescent in water (ΦF = 15.4%). Due to the well-known thermo-responsive character of NIPAM, P(NIPAM-co-NI-2TPE) exhibits an interesting fluorescence change in response to various temperatures. Due to the thermo-induced shrinking of the PNIPAM chain, the fluorescence intensity gradually increased from 20 to 34 °C. As the temperature further increased from 34 to 90 °C, the fluorescence intensity decreased sharply, which was caused by the well-known thermal effects. Furthermore, we synthesized a P(HEA-co-NI-2TPE–TPP acrylate) copolymer, in which HEA is a hydrophilic unit, TPP is a mitochondria label and NI-2TPE a fluorescent probe. The corresponding polymer probe is highly soluble in water with FLQY = 7% and we have further applied this probe as a mitochondria targeted imaging tracker in HeLa cells successfully.

Folic acid-functionalized up-conversion nanoparticles: toxicity studies in vivo and in vitro and targeted imaging applications

Nanoscale ◽  
2014 ◽  
Vol 6 (15) ◽  
pp. 8878-8883 ◽  
Author(s):  
Lining Sun ◽  
Zuwu Wei ◽  
Haige Chen ◽  
Jinliang Liu ◽  
Jianjian Guo ◽  
...  
Keyword(s):  
Folic Acid ◽  
Hela Cells ◽  
Imaging Agents ◽  
Targeted Imaging ◽  
Nanoparticle Imaging ◽  
Good Biocompatibility ◽  
Low Cytotoxicity ◽  
Nanoparticles Toxicity

Three kinds of folate-targeted up-conversion nanoparticle imaging agents were prepared. These nanoparticles show good biocompatibility and up-conversion luminescence emission in water, and low cytotoxicity in vivo and in vitro. They were successfully applied to the targeted imaging of HeLa cells.

Platinum Compounds as Stains for Electron Microscopy

1974 ◽  
Vol 32 ◽  
pp. 230-231
Author(s):  
S. K. Aggarwal ◽  
P. McAllister ◽  
R. W. Wagner ◽  
B. Rosenberg
Keyword(s):  
Electron Microscopy ◽  
Hela Cells ◽  
Uranyl Acetate ◽  
Sarcoma 180 ◽  
Platinum Compounds ◽  
Kb Cells ◽  
En Bloc ◽  
Human Lymphoma ◽  
Ultrathin Sections ◽  
Blue Coloration

Uranyl acetate has been used as an electron stain for en bloc staining as well as for staining ultrathin sections in conjunction with various lead stains (Fig. 1). Present studies reveal that various platinum compounds also show promise as electron stains. Certain platinum compounds have been shown to be effective anti-tumor agents. Of particular interest are the compounds with either uracil or thymine as one of the ligands (cis-Pt(II)-uracil; cis-Pt(II)-thymine). These compounds are amorphous, highly soluble in water and often exhibit an intense blue coloration. These compounds show enough electron density to be used as stains for electron microscopy. Most of the studies are based on various cell lines (human AV, cells, human lymphoma cells, KB cells, Sarcoma-180 ascites cells, chick fibroblasts and HeLa cells) while studies on tissue blocks are in progress.

Electron Microscopy of Mycoplasma Pneumoniae

1971 ◽  
Vol 29 ◽  
pp. 258-259 ◽  
Author(s):  
E. S. Boatman ◽  
G. E. Kenny
Keyword(s):  
Electron Microscopy ◽  
Light Microscopy ◽  
Growth Phase ◽  
Hela Cells ◽  
Sulfonic Acid ◽  
Gamma Globulin ◽  
Horse Serum ◽  
Morphological Aspects ◽  
The Family

Information concerning the morphology and replication of organism of the family Mycoplasmataceae remains, despite over 70 years of study, highly controversial. Due to their small size observations by light microscopy have not been rewarding. Furthermore, not only are these organisms extremely pleomorphic but their morphology also changes according to growth phase. This study deals with the morphological aspects of M. pneumoniae strain 3546 in relation to growth, interaction with HeLa cells and possible mechanisms of replication.The organisms were grown aerobically at 37°C in a soy peptone yeast dialysate medium supplemented with 12% gamma-globulin free horse serum. The medium was buffered at pH 7.3 with TES [N-tris (hyroxymethyl) methyl-2-aminoethane sulfonic acid] at 10mM concentration. The inoculum, an actively growing culture, was filtered through a 0.5 μm polycarbonate “nuclepore” filter to prevent transfer of all but the smallest aggregates. Growth was assessed at specific periods by colony counts and 800 ml samples of organisms were fixed in situ with 2.5% glutaraldehyde for 3 hrs. at 4°C. Washed cells for sectioning were post-fixed in 0.8% OSO4 in veronal-acetate buffer pH 6.1 for 1 hr. at 21°C. HeLa cells were infected with a filtered inoculum of M. pneumoniae and incubated for 9 days in Leighton tubes with coverslips. The cells were then removed and processed for electron microscopy.

Use of Uranium-Labeled Antibodies for Electron Microscopic Localization of Various Antigens in Mammalian Cells

1967 ◽  
Vol 25 ◽  
pp. 136-137
Author(s):  
J. P. Petrali ◽  
E. J. Donati ◽  
L. A. Sternberger
Keyword(s):  
Endoplasmic Reticulum ◽  
Hela Cells ◽  
Mammalian Cells ◽  
Specific Antiserum ◽  
Electron Microscopic ◽  
E Coli ◽  
Cytoplasmic Membranes ◽  
Viral Progeny ◽  
Ultrathin Sections ◽  
Electron Microscopic Localization

Specific contrast is conferred to subcellular antigen by applying purified antibodies, exhaustively labeled with uranium under immunospecific protection, to ultrathin sections. Use of Seligman’s principle of bridging osmium to metal via thiocarbohydrazide (TCH) intensifies specific contrast. Ultrathin sections of osmium-fixed materials were stained on the grid by application of 1) thiosemicarbazide (TSC), 2) unlabeled specific antiserum, 3) uranium-labeled anti-antibody and 4) TCH followed by reosmication. Antigens to be localized consisted of vaccinia antigen in infected HeLa cells, lysozyme in monocytes of patients with monocytic or monomyelocytic leukemia, and fibrinogen in the platelets of these leukemic patients. Control sections were stained with non-specific antiserum (E. coli).In the vaccinia-HeLa system, antigen was localized from 1 to 3 hours following infection, and was confined to degrading virus, the inner walls of numerous organelles, and other structures in cytoplasmic foci. Surrounding architecture and cellular mitochondria were unstained. 8 to 14 hours after infection, antigen was localized on the outer walls of the viral progeny, on cytoplasmic membranes, and free in the cytoplasm. Staining of endoplasmic reticulum was intense and focal early, and weak and diffuse late in infection.

Exogeneous factors are not necessary in attachment, spreading and polarization of hela cells

1978 ◽  
Vol 36 (2) ◽  
pp. 310-311
Author(s):  
W. Liebrich
Keyword(s):  
Hela Cells ◽  
Calf Serum ◽  
Glass Tube ◽  
Serum Free Medium ◽  
Nacl Solution ◽  
Free Medium ◽  
Minimum Essential Medium ◽  
Serum Free ◽  
All Solutions ◽  
Glass Support

HeLa cells were grown for 2-3 days in EAGLE'S minimum essential medium with 10% calf serum (S-MEM; Seromed, München) and then incubated for 24 hours in serum free medium (MEM). After detaching the cells with a solution of 0. 14 % EDTA and 0. 07 % trypsin (Difco, 1 : 250) they were suspended in various solutions (S-MEM = control, MEM, buffered salt solutions with or without Me++ions, 0. 9 % NaCl solution) and allowed to settle on glass tube slips (Leighton-tubes). After 5, 10, 15, 20, 25, 30, 1 45, 60 minutes 2, 3, 4, 5 hours cells were prepared for scanning electron microscopy as described by Paweletz and Schroeter. The preparations were examined in a Jeol SEM (JSM-U3) at 25 KV without tilting.The suspended spherical HeLa cells are able to adhere to the glass support in all solutions. The rate of attachment, however, is faster in solutions without serum than in the control. The latter is in agreement with the findings of other authors.

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