Pharmacological screening of polyherbal hepatoprotective -‘Doctor’s LIVAFIT’

2020 ◽  
Vol 8 (1) ◽  
pp. 6-11
Author(s):  
Avinash Kumar Reddy G ◽  
Jamal Basha S
Keyword(s):  
Chronic Alcoholism ◽  
Hepatoprotective Activity ◽  
Hepatic Regeneration ◽  
Herbal Extracts ◽  
Synthetic Drugs ◽  
Hepatic Diseases ◽  
Pharmacological Screening ◽  
Sacred Science ◽  
Dose Dependent ◽  
The Needy

Considering the importance of ayurvedic herbs, science behind their potential in treating diseases and advantages over synthetic medicines in limiting side effects, there is an urgent need to employ such sacred science to produce medicines that effectively cure diseases and to distribute them to the needy. Many herbs mentioned in ayurveda are used to treat dreadful hepatic diseases especially, cirrhosis which is caused by the liver damage due to toxins, chronic alcoholism, synthetic drugs etc. In the present study, poly herbal hepatoprotective formulation, LIVAFIT, had been prepared and standardized for determining its quality and safety. Methanol herbal extracts were formulated according to a formula with Phyllanthus neruri, Picorrhiza kurroa, Andrographis paniculata as active ingredients and various other herbs helpful for possible hepatic regeneration and preventing hepatotoxicity. Physico chemically, microscopically and chemically standardized extracts were used in the study. Following the results of acute toxicity studies and after confirming the safety of extracts, hepatoprotective activity of formulation was evaluated by CCL4 induced hepatotoxicity method in albino wistar rats. It exhibited a dose dependent significant (P<0.001) reduction in hepatotoxicity when compared with that of standard, Silymarin and a pre-marketed ayurvedic product.

Biological and Phytochemical Screening of Tephrosia purpurea extract on Chemically Induced Hepatocellular Carcinoma with Reference to Biochemical Parameters

2019 ◽  
Vol 10 (02) ◽  
Author(s):  
Irfan Aziz ◽  
Birendra Shrivastava ◽  
Chandana Venkateswara Rao ◽  
Sadath Ali
Keyword(s):  
Free Radicals ◽  
Ethanolic Extract ◽  
Hepatoprotective Activity ◽  
Control Group ◽  
Marker Enzymes ◽  
Tephrosia Purpurea ◽  
Single Intraperitoneal Injection ◽  
Chemically Induced ◽  
Ccl4 Treatment ◽  
Dose Dependent

Tephrosia purpurea possesses hepatoprotective activity as evidenced by the significant and dose dependent restoring the activities of entire liver cancer marker enzymes, diminution in tumor incidence, decrease in lipid peroxidation (LPO) and increase in the level of antioxidant enzymes (GSH, CAT, SOD, GPx and GST) through scavenging of free radicals, or by enhancing the activity of antioxidant, which then detoxify free radicals. These factors protect cells from ROS damage in NDEA and CCl4-induced hepatocarcinogenesis. Histopathological observations of liver tissues too correlated with the biochemical observations. Thus, present investigation suggested that the Tephrosia purpurea would exert a chemoprotective effect by reversing the oxidant-antioxidant imbalance during hepatocarcinogenesis induced by NDEA and CCl4. Besides Tephrosia purpurea is very much effective in preventing NDEA-induced multistage hepatocarcinogenesis possibly through antioxidant and antigenotoxic nature, which was confirmed by various liver injury and biochemical tumour markers enzymes. The hepatoprotective activity of aTephrosia purpurea of 50 % ethanolic extract was studied using rats. The animals received a single intraperitoneal injection of N-nitrosodiethylamine 200mg/kg body wt followed by subcutaneous injection of CCl4 in a dose of 3 ml/kg body wt.Tephrosia purpureaextract dose dependently and significantly the increase in serum hepatic enzyme levels after NDEAand CCl4 treatment compared to the toxin control group. The results of this study confirmed the antioxidant and hepatoprotective activity of the Tephrosia purpurea extract against carbon tetrachlorideand N-nitrosodiethylamine induced hepatotoxicity in rats.

PHYTOCHEMICAL AND BIOLOGICAL STUDIES OF ROTULA AQUATICA LOUR. ROOTS

INDIAN DRUGS ◽  
2013 ◽  
Vol 50 (11) ◽  
pp. 34-38
Author(s):  
T. Shyam ◽  
◽  
S Ganapaty
Keyword(s):  
Body Weight ◽  
Spectroscopic Data ◽  
Methanolic Extract ◽  
Hepatoprotective Activity ◽  
Dependent Manner ◽  
Biological Studies ◽  
Bacteria And Fungi ◽  
Dose Levels ◽  
Dose Dependent ◽  
Chemical Evidence

Four compounds viz α-amyrin, β- amyrin, bauerenol and ellagic acid were isolated from the methanolic extract of Rotula aquatica roots. The structures of these compounds were elucidated on the basis of spectroscopic data analysis and chemical evidence. The extract was evaluated for hepatoprotective activity against carbon tetrachloride induced hepatotoxic model at a dose levels of 200,400 and 800 mg/ kg body weight and compared with that of the standard silymarin (25mg/kg body weight). It showed good hepatoprotective activity in a dose dependent manner. The extract was also screened for antimicrobial activity against various types of organisms like bacteria and fungi.

scholarly journals HEPATOPROTECTIVE EVALUATION OF EPALTES DIVARICATA (L.) CASS. WHOLE PLANT EXTRACTS AGAINST PARACETAMOL-INDUCED HEPATOTOXICITY IN RATS

2016 ◽  
Vol 8 (12) ◽  
pp. 231 ◽  
Author(s):  
K. Amala ◽  
R. Ilavarasan ◽  
R. Arunadevi ◽  
S. Amerjothy
Keyword(s):  
Aqueous Extract ◽  
Histopathological Examination ◽  
Hepatoprotective Activity ◽  
Hepatoprotective Effect ◽  
Dependent Manner ◽  
Traditional Use ◽  
Whole Plant ◽  
Medicinal Use ◽  
Histopathological Studies ◽  
Dose Dependent

<p><strong>Objective: </strong>The plant of <strong><em>Epaltes</em></strong><strong> <em>divaricata </em>(L.) </strong>Cass.<strong> Traditionally used for jaundice. </strong>The present work aimed to investigate the hepatoprotective activity of alcohol and aqueous extract of the whole plant against paracetamol-induced hepatotoxicity in rats to substantiate its traditional use.</p><p><strong>Methods: </strong>The alcohol and aqueous (200 and 400 mg/kg) extract of <em>Epaltes divaricata</em> prepared by cold maceration were administered orally to the animals with hepatotoxicity induced by paracetamol (1000 mg/kg). Silymarine (40 mg/k) was given as reference standard. Hepatoprotective activity was assessed by estimating marker enzymes and by histopathological studies.</p><p><strong>Results: </strong>Both alcohol and aqueous (200 and 400 mg/kg) extract treatment significantly restored the paracetamol-induced elevations in levels of serum enzymes aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphate (ALP) and total bilirubin in a dose-dependent manner. Histopathological examination revealed that the treatment attenuated the paracetamol-induced damage to the liver. The hepatoprotective effect of both extracts was comparable to that of the standard hepatoprotective agent, silymarin.</p><p><strong>Conclusion: </strong>The alcohol and aqueous extract of <em>E. divaricata</em> exhibited hepatoprotective effect against paracetamol-induced liver damage in rats. This study also validated their traditional medicinal use in jaundice.</p>

scholarly journals Evaluation of hepatoprotective potential of aqueous extract of Withania somnifera in albino rats

2021 ◽  
Vol 10 (3) ◽  
pp. 255
Author(s):  
Monica Sharma ◽  
Anand Gaur ◽  
Pinki Vishwakarma ◽  
Raj Kumar Goel ◽  
K. K. Saxena
Keyword(s):  
Aqueous Extract ◽  
Withania Somnifera ◽  
Work Force ◽  
Hepatoprotective Activity ◽  
Albino Rats ◽  
Standard Drug ◽  
Hepatic Diseases ◽  
Organ Systems ◽  
Herbal Plant ◽  
Biochemical Examination

Background: Hepatic diseases are a major cause of morbidity and disability of work force throughout the world. The treatment of hepatic diseases with standard drugs poses the risk of toxicity on various organ systems. Withania somnifera, a herbal plant has been claimed to be effective in the treatment of various types of hepatic conditions. The present study was undertaken to explore the hepatoprotective activity of aqueous extract of Withania somnifera (AEWS) in experimentally induced hepatotoxicity in albino rats.Methods: The study was commenced after obtaining approval from institutional animal ethical committee using AEWS leaves in Albino wistar rats (150-200 gm) of either sex. The hepatoprotective activity was evaluated using biochemical examination. The animals were divided into five groups of six animals each. In each experiment, first group was given normal saline (1 ml/kg/day), second group was injected with toxin CCl4 (1 ml/kg) i.p only once to produce hepatotoxicity, third and fourth groups were given Withania somnifera orally (500 mg/kg and 1000 mg/kg) (respectively), as a single dose per orally every morning and fifthgroup was given standard drug Liv-52 (1 mg/kg).Results: Aqueous extract of Withania somnifera leaves in oral dose exhibited significant hepatoprotective effect in all models used in this study.Conclusions: It can be concluded from our study that aqueous extract of Withania somnifera leaves possesses hepatoprotective activity.

HEPATOPROTECTIVE ACTIVITY OF AERIAL PARTS OF NELUMBO NUCIFERA GAERTN. IN PARACETAMOL AND ISONIAZID-RIFAMPICINE INDUCED HEPATOTOXICITY IN RATS

INDIAN DRUGS ◽  
2013 ◽  
Vol 50 (12) ◽  
pp. 47-53
Author(s):  
D Gupta ◽  
Keyword(s):  
Synergistic Effect ◽  
Group Treatment ◽  
Hepatoprotective Activity ◽  
Nelumbo Nucifera ◽  
Control Group ◽  
Aqueous Extracts ◽  
Aerial Parts ◽  
Dose Dependent

Hepatoprotective activity of ethanolic and aqueous extracts (200 and 400 mg/kg b.w.) of Nelumbo nucifera Gaertn. (NNE and NNA respectively) was evaluated by paracetamol and Isoniazid-rifampicine (INH-RMP) induced hepatotoxicity models. In paracetamol induced hepatoxicity model, NNA 400 and NNE 400 produced significant (p<0.005) reduction in SGOT level when compared to that of control group. Treatment with NNE 200, NNE 400 and NNA 400 produced significant reduction (p<0.01) in SGPT levels when compared to that of control group. SALP levels were significantly (p<0.001) reduced in animals treated with Silymarin (standard), ethanolic and aqueous extracts at both doses when compared to that of control. In INH-RMP induced hepatotoxicity model, significant (p<0.001) reduction in SGOT levels was produced in animals treated with NNA 200 and 400, NNE 200 and 400, when compared to control group animals. Ethanolic and aqueous extracts of Nelumbo nucifera at both doses produced significant (p<0.05 and 0.001 respectively) decrease in the SGPT and SALP levels when compared with control group. Hepatoprotective activity was found to be dose dependent. Significant hepatoprotective activity produced by ethanolic & aqueous extracts of Nelumbo nucifera may be a consequence of synergistic effect of the constituents present in the extracts namely, β-sitosterol and flavonoids (kaempferol, quercetin etc.) The results suggest that the ethanolic and aqueous extracts (NNE and NNA) of Nelumbo nucifera aerial parts possess significant hepatoprotective activity.

Evaluation of Hepatoprotective activity of Methanol extract of Persicaria hydropiper: An in vivo screening

2021 ◽  
pp. 5819-5824
Author(s):  
Pooja Kamra ◽  
Mahaveer Singh ◽  
Hardarshan Singh Lamba ◽  
Birendra Srivastava
Keyword(s):  
Carbon Tetrachloride ◽  
Intraperitoneal Administration ◽  
Hepatoprotective Activity ◽  
Dependent Manner ◽  
Standard Drug ◽  
Whole Plant ◽  
Dose Dependent ◽  
Histopathological Investigation ◽  
Different Doses

The present study aimed to evaluate the hepatoprotective potential of methanolic whole plant extract of Persicaria hydropiper in carbon tetrachloride (CCl4) induced hepatotoxicity model. Hepatotoxicity was induced in rats by intraperitoneal administration of carbon tetrachloride (CCl4) for seven days. The extract was thereafter administered at two different doses of 200 mg/kg and 400 mg/kg body weight for next seven days. Silymarin was used as a reference standard. The extract revealed hepatoprotective activity in dose dependent manner. The dose of 400 mg/kg exhibited maximum hepatoprotective ability as apparent from several evaluation parameters including liver function profile, bilirubin, antioxidant enzymes as well as histopathological investigation which was comparable to the standard drug Silymarin respectively. These findings sustenance the use of the extract as an adjuvant with existing therapy for treatment of liver ailments.

EVALUATION OF HEPATOPROTECTIVE EFFECTS OF SELECTED FRUIT PEEL EXTRACTS AND THEIR POLY HERBAL MIXTURE ON CCL4 INDUCED HEPATOTOXICITY: AN IN VIVO STUDY

INDIAN DRUGS ◽  
2020 ◽  
Vol 57 (09) ◽  
pp. 67-74
Author(s):  
Gana Manjusha Kondepudi ◽  
Battu Ganga Rao ◽  
P Balakrishnaiah
Keyword(s):  
Hepatoprotective Activity ◽  
Serum Markers ◽  
Albino Rats ◽  
Dependent Manner ◽  
Fruit Peel ◽  
Herbal Mixture ◽  
Wistar Albino Rats ◽  
Dose Dependent ◽  
Peel Extract

The main aim of this study was to screen the selected fruit peel extracts and their polyherbal mixture (PHM) for hepatoprotective activity. Male wistar albino rats (180-200 g), divided into 12 groups after induction of hepatotoxicity, were treated with selected fruit peel extracts and PHM and at the end of 14th day blood and liver samples were collected and analysed. The aqueous peel extract of Malus pumila was a better hepatoprotective among the selected peel extracts. The activities might be due to the conditioning of hepatocytes by protecting the integrity of the membrane from CCl4 induced leakage of serum markers into circulation. All the selected plant extracts and PHM were shown to revert back the liver enzymes to the normal values in diseased rats in a dose dependent manner. In conclusion, the selected fruit peel extracts and poly herbal mixture can be a potent hepatoprotective agent due to their antioxidant and anti-inflammatory actions.

scholarly journals Isolation, characterization and evaluation of anti-proliferative properties of andrographolide isolated from Andrographis paniculata on cultured HaCaT cells

2021 ◽  
Vol 67 (1) ◽  
pp. 35-45
Author(s):  
Shammy Jindal ◽  
Rajendra Awasthi ◽  
Dhananjay Singare ◽  
Giriraj T. Kulkarni
Keyword(s):  
Cell Model ◽  
Inhibitory Effect ◽  
Epidermal Keratinocytes ◽  
Herbal Extracts ◽  
Inflammatory Skin Disease ◽  
Inflammatory Infiltration ◽  
Synthetic Drugs ◽  
Significant Inhibitory Effect ◽  
Hacat Keratinocyte ◽  
First Time

Summary Introduction: Psoriasis is an inflammatory skin disease characterized by hyper-proliferation, abnormal epidermal keratinocytes and inflammatory infiltration. It affects approximately 4% of the population globally. Herbal extracts have better results with less toxic effects than the synthetic drugs in the treatment of psoriasis. Objective: Present study was aimed to access the anti-psoriatic effect of andrographolide extracted from Andrographis paniculate (A. paniculata). Method: We extracted, characterized, and screened the extracted andrographolide for anti-proliferative characteristics using cultured cell model of human HaCaT keratinocyte. Results: Andrographolide at 31.25 µg/mL (90 µM) demonstrated significant inhibitory effect on human HaCaT keratinocytes proliferation in cell culture. To our best knowledge, we reported the anti-proliferative potency of andrographolide extracted from A. paniculata for the first time. Conclusion: The results suggest that the andrographolide extracted from A. paniculata plant may have potential to be used in the management of psoriasis.

scholarly journals Antioxidant and protective effect of hydroalcoholic extract of Celtis australis L. on CCl4 induced hepatotoxicity

2019 ◽  
Author(s):  
Mohammad Shokrzadeh ◽  
Hossein Bakhshi jouybari ◽  
Majedeh Hosseinpour ◽  
Ali Ziar ◽  
Emran Habibi
Keyword(s):  
Biochemical Parameters ◽  
Radical Scavenging ◽  
Hepatoprotective Activity ◽  
Hepatic Tissue ◽  
Serum Biochemical Parameters ◽  
Destructive Effect ◽  
Hydroalcoholic Extract ◽  
Hepatic Diseases ◽  
Serum Biochemical ◽  
Celtis Australis

Several human diseases including cancer, rheumatoid arthritis, and neurodegenerative and hepatic diseases are related to the destructive effect of reactive oxygen species (ROS). Antioxidants may provide a possible solution to this problem. This study was carried out to investigate the effect of hydroalcoholic extract of Celtis australis on CCl4 induced hepatotoxicity in mice. The antioxidant activity of C. australis was evaluated by 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay. For evaluation of hepatoprotective activity of the extract, the animals were pretreated with 200 and 400 mg/kg of C. australis extract intraperitoneally for 7 days and then received CCl4 (0.5 ml/kg in olive oil). Liver injury was determined by serum biochemical parameters such as Alanine aminotransferase (ALT), Aspartate aminotransferase (AST), Alkaline phosphatase (ALP), glutathione contents of liver tissue, and histopathological studies. A significant reduction in the serum biochemical parameters was observed when compared to the CCl4 receiving group. The standard antioxidant used in the study was ascorbic acid. C. australis extract significantly suppressed the increase in plasma activities of liver enzymes and effectively protected animals against CCl4-induced hepatic tissue damages. This study confirmed the hepatoprotective effect of the hydroalcoholic extract of C. australis.

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