Detection of prevalence of antinuclear antibodies in clinically suspected cases of systemic autoimmune diseases by indirect immunofluorescence test in a tertiary care hospital

2019 ◽  
Vol 9 (2) ◽  
pp. 23-27
Author(s):  
Jabeen Begum ◽  
◽  
V V Shailaja ◽  
Rajeshwar Rao ◽  
◽  
...  
Keyword(s):  
Autoimmune Diseases ◽  
Indirect Immunofluorescence ◽  
Antinuclear Antibodies ◽  
Tertiary Care Hospital ◽  
Tertiary Care ◽  
Care Hospital ◽  
Immunofluorescence Test ◽  
Systemic Autoimmune Diseases ◽  
Indirect Immunofluorescence Test

scholarly journals Detection of antinuclear antibodies by indirect immunofluorescence method and its comparison with line immunoassay in a tertiary care hospital: A laboratory based observational study

2014 ◽  
Vol 2 (4) ◽  
pp. 194-199
Author(s):  
Madhavi Latha B ◽  
Anil Kumar B
Keyword(s):  
Autoimmune Diseases ◽  
Indirect Immunofluorescence ◽  
Screening Test ◽  
Antinuclear Antibodies ◽  
Tertiary Care ◽  
Screening Method ◽  
Care Hospital ◽  
Cell Nuclei ◽  
Serum Samples ◽  
Autoantibody Production

Antinuclear antibodies (ANA) are the hallmark of autoantibody production in autoimmune diseases and its testing is widely used as screening test in autoimmune diseases. ANA are directed against components of the cell nuclei such as DNA, histones, nucleoli and ribonucleoprotein. ANA are detected by indirect immunofluorescence (IIF) assay, which is among the most commonly used routine method for ANA detection as screening test, due to its ability to detect multiple antigens simultaneously. In this study, serum samples, referred to our laboratory for ANA testing were subjected for testing by IIF method and line immunoassay (LIA) during a study period of 20 months and the two were correlated with one another to establish any link between the two. A total of 279 serum samples were processed for ANA testing during the study period from June 2012 to January 2014. Of these 279 samples, 199(71.3%) were ANA IIF positive and 80(28.7%) were ANA IIF negative. The spectrum of various positive ANA IIF patterns are nucleus homogenous 52(26.1%), nucleus granular 50(25.1%), mixed pattern 57(28.6%), mitosis positive 15(7.5%), nucleus nucleolar 13(6.5%), nucleus dotted 8(4%), nuclear membrane 1(0.5%), cytoplasm positive 3(1.5%). All the samples tested by ANA IIF were subjected to LIA. Of these 159(56.9%) were both ANA IIF and LIA positive. In addition, 40(14.3%) samples were detected as IIF positive but LIA negative, whereas the rest 14(5%) samples were IIF negative but LIA positive. In the present study, a definite correlation was found in 201(71%) samples between ANA patterns and the LIA. Thus ANA IIF method using biochips can be used as a cost effective screening method for ANA testing and restricting LIA, which are expensive. This could economize on the cost of laboratory investigations in a developing country like India.

Uveitis in Adult Patients with Rheumatic Inflammatory Autoimmune Diseases at a Tertiary-care Hospital in Mexico City

2010 ◽  
Vol 38 (2) ◽  
pp. 325-330 ◽  
Author(s):  
FRANCISCO JAVIER JIMÉNEZ-BALDERAS ◽  
GEMA FERNANDEZ-ARRIETA ◽  
ADOLFO CAMARGO-CORONEL ◽  
MIGUEL ÁNGEL AKE-UC ◽  
MIGUEL ANGEL VAZQUEZ-ZARAGOZA ◽  
...  
Keyword(s):  
Autoimmune Diseases ◽  
Mexico City ◽  
Behcet’S Disease ◽  
Tertiary Care Hospital ◽  
Behçet's Disease ◽  
Tertiary Care ◽  
Seasonal Distribution ◽  
Adult Patients ◽  
Care Hospital ◽  
Inflammatory Autoimmune Diseases

Objective.Our aim is to describe the frequency of uveitis associated with rheumatic inflammatory autoimmune diseases (RIAD) in adult patients admitted to the Rheumatology Department at a tertiary-care hospital in Mexico City. We also describe the clinical features, seasonal distribution, treatment, and ocular complications associated with this disease.Methods.We reviewed 1332 charts of patients with RIAD and selected those that had a diagnosis of uveitis. We obtained the following data: age, sex, type of uveitis and relationship with diagnosis of RIAD, recurrences, seasonal distribution, treatment, and residual visual deficit.Results.We found 57 (4.27%) cases of uveitis in 1332 charts, including 38 men and 19 women (M:F ratio 2:1), aged 47 ± 16 years. Nongranulomatous acute anterior uveitis (NGAAU) comprised 90.52% of cases (52/57). In 64.91% of cases (37/57), uveitis preceded the diagnosis of RIAD by 12 ± 9 years, more frequently in winter (35.96%; p = NS). Uveitis was found in 40/93 patients with ankylosing spondylitis (AS), in 7/11 patients with relapsing polychondritis (RP), in 8/16 patients with Behçet’s disease, in 1/16 patients with polyarteritis nodosa, and in 1/590 patients with rheumatoid arthritis (RA). Ninety-six percent of the patients were treated with steroids. Upon a mean followup of 60 days (range 7–4745 days), reduction of visual acuity (≤ 20/200) was associated with recurrence of uveitis in 3/7 cases with AS, in 4/8 cases with Behçet’s disease, in 3/7 with RP, and in 1 case of uveitis and seronegative RA.Conclusion.NGAAU frequently precedes RIAD and is found predominately in men, with a tendency to occur in winter.

Confidence toward vaccination as reported by parents of children admitted to a tertiary care hospital in Riyadh, Saudi Arabia: A cross sectional study

Vacunas ◽  
2020 ◽  
Vol 21 (2) ◽  
pp. 95-104 ◽  
Author(s):  
Y.M. AlGoraini ◽  
N.N. AlDujayn ◽  
M.A. AlRasheed ◽  
Y.E. Bashawri ◽  
S.S. Alsubaie ◽  
...  
Keyword(s):  
Saudi Arabia ◽  
Tertiary Care Hospital ◽  
Tertiary Care ◽  
Cross Sectional Study ◽  
Care Hospital ◽  
Sectional Study ◽  
Cross Sectional

TO DETERMINE THE ROLE OF CA125 IN THE DIAGNOSIS OF ACUTE APPENDICITIS

2020 ◽  
Vol 4 (7) ◽  
Author(s):  
Vinod Kumar ◽  
Bhupen Songra ◽  
Richa Jain ◽  
Deeksha Mehta
Keyword(s):  
Acute Appendicitis ◽  
Clinical Diagnosis ◽  
Tertiary Care Hospital ◽  
Tertiary Care ◽  
Ca 125 ◽  
Care Hospital ◽  
Serum Samples ◽  
Roc Curve Analysis ◽  
Surgery Department

Background: the present study was under taken to determine the role of CA-125 in the diagnosis of acute appendicitis (AA), to prevent its complications and also in preventing negative appendicectomies in tertiary care hospital. Methods: The study was conducted at a tertiary care and research center between 01/03/2018 to 30/06/2019. Patients admitted to the surgery department with diagnosis of AA were considered for the study. After informed consent, a, standardized history was obtained as a case Performa. Serum samples from all the cases with clinical diagnosis of AA were obtained and stored. Only the cases with histopathologically approved AA were included in the study. Cases operated for clinical diagnosis of AA, but not histopathologically proven AA was not included in the study. CA125 levels in cases with definitive diagnosis of AA were measured. Results: In present study, ROC curve analysis revealed the sensitivity of 87.27 % and specificity of 90.91 % when the CA 125 cut-off value of > 16.8 was taken to diagnose acute appendicitis. AUC was 0.911 with a standard error of 0.0292. Conclusion: In this study we have observed that CA125 showed a positive correlation with acute appendicitis, that was statistically not significant (P>0.05). We didn’t evaluate the correlation with the disease severity. We consider that CA125 can be used as a marker in acute appendicitis cases although further research is still needed. Keywords: CA125, Acute Appendicitis, Surgery.

Sign in / Sign up

Export Citation Format

Share Document