scholarly journals Features of morphological changes of the uterus after the death prescription

Morphologia ◽  
2021 ◽  
Vol 14 (4) ◽  
pp. 90-94
Author(s):  
E. K. Grygorian ◽  
M. S. Myroshnychenko
Keyword(s):  
Morphological Changes ◽  
Uterine Prolapse ◽  
Muscle Fibers ◽  
Postmortem Changes ◽  
Time Intervals ◽  
Biopsy Material ◽  
Tissue Samples ◽  
Judicial Practice ◽  
Development Methods ◽  
Fibroblastic Cells

Background. Assessing the postmortem interval (PMI) is one of the most problematic issues in judicial practice. Objective. To investigate the morphological changes of uterus tissues to determine the PMI-dependent features of postmortem changes development. Methods. A morphological analysis of 40 uterus tissue samples was performed; consisting of two groups: first group – samples from the corpses of women aged 23 to 70 years (n = 34), second group – comparison group – biopsy material from 6 women with uterine prolapse, uterine leiomyoma, from unaffected areas. Results. In the period from 24 to 48 hours after death, there were changes in the form of a slight change in the structure of cellular elements, changes in their color, the presence of light gaps between connective tissue and muscle fibers. In the period from 48 to 72 hours, the destruction of the border between the muscular and serous membranes, blurred contours and ruptures of cells, severe swelling of muscle fibers, wide gaps between muscle fibers, a significant decrease in fibroblastic cells were observed. In addition, there was no endothelium in a significant number of vessels, loss of clarity of fiber bundles, lack of nuclei in a significant number of myocytes. Desquamed endothelial cells and hemolyzed erythrocytes were seen in the openings of most vessels. Conclusion. Histological examination of uterine tissues showed the presence of specific changes during the studied time intervals after death, which can be used to introduce criteria to determine the time since death in practice.

scholarly journals MORPHOLOGICAL FEATURES OF THE UTERUS IN WOMEN AT DIFFERENT TIME INTERVALS OF THE POSTMORTEM PERIOD AS DIAGNOSTIC CRITERIA FOR ESTABLISHING THE POSTMORTEM INTERVAL

2021 ◽  
Vol 74 (4) ◽  
pp. 821-827
Author(s):  
Vasil O. Olkhovsky ◽  
Edgar K. Grygorian ◽  
Mykhailo S. Myroshnychenko ◽  
Sergii V. Kozlov ◽  
Kostiantyn M. Suloiev ◽  
...  
Keyword(s):  
Diagnostic Criteria ◽  
Uterine Prolapse ◽  
Postmortem Interval ◽  
Morphological Features ◽  
Postmortem Changes ◽  
Structural Elements ◽  
Autopsy Material ◽  
Time Intervals ◽  
Outer Membranes ◽  
Vascular Walls

The aim is to identify the morphological features of the uterus layers in women at different time intervals of the postmortem period as diagnostic criteria for establishing the postmortem interval. Materials and methods: In the study we used surgical and autopsy material – uterine tissue fragments. All materials were divided into two groups. The 1st group (G 1) included surgical material from women (n=6) who underwent removal of the uterus, or uterus with the appendages due to leiomyoma, uterine prolapse. The 2nd group (G 2) included autopsy material from 42 women with known causes of death and postmortem period (from 24 to 48 hours – 6 cases, from 49 to 72 hours – 7 cases, from 73 to 96 hours – 8 cases, from 97 to 120 hours – 6 cases, from 121 to 144 hours – 8 cases, more than 144 hours – 7 cases). Histological and immunohistochemical study methods were used. Results: A comprehensive morphological study of the women uterus revealed a time-dependent increase of postmortem changes in this organ linked with the increase of postmortem period. In cases of postmortem period duration up to 144 hours, the structural elements of the uterine layers were identified. In cases where the duration of the postmortem period was more than 145 hours, microscopically the uterus was represented by eosinophilic fibrous or dusty masses, the histogenesis of which could not be determined. The processes of autolysis occurred more intensely and faster in the mucous membrane of the uterus, in comparison with the muscular and serous membranes, and in the vessels – in their inner membrane, in comparison with the middle and outer membranes. Autolytic changes in the muscular membrane of the uterus and vascular walls occurred more intensely in muscle fibers compared to connective tissue fibers. Conclusions: The histological and immunohistochemical features of the women uterus at different postmortem periods have a certain forensic medical significance and can be used for establishing the postmortem interval.

Last second twitch-response test of single intact skeletal muscle fibers prior to quick-freezing after various stimulation intervals

1983 ◽  
Vol 41 ◽  
pp. 526-527
Author(s):  
J.R. Sommer ◽  
R. Nassar ◽  
I. Taylor
Keyword(s):  
Skeletal Muscle ◽  
Time Course ◽  
Morphological Changes ◽  
Muscle Fibers ◽  
Visual Observation ◽  
Time Interval ◽  
Time Intervals ◽  
Twitch Response ◽  
Skeletal Muscle Fibers ◽  
Quick Freezing

Conventional chemical fixation of muscle fibers is not suited to disclose morphological changes occurring with a time course of only a few msec, e.g.during excitation-contraction-coupling. We have taken advantage of a quick-freeze method to be able to study single intact frog skeletal muscle fibers (r.temporaria) after various time intervals following electrical stimulation. The electronics were designed to permit any time interval from 0 to more than 1 sec between stimulation and impact of the specimen on a liquid He-cooled copper block. It is important to monitor the twitch-response to stimulation. Given the geometry of the freezing device ("Slammer", Polaron), and the fact that the device does not operate vibration-free, it is quite difficult to design and built an effective monitor able to record the actual twitch-response following the stimulation of an isolated single muscle fiber during its descent prior to freezing. We have built a simple device that allows visual observation of the twitch-response within a few seconds prior to the definitive stimulation during the specimen drop.

Effects of Aging on Type II Muscle Fibers: A Systematic Review of the Literature

2007 ◽  
Vol 15 (3) ◽  
pp. 336-348 ◽  
Author(s):  
Florian Brunner ◽  
Annina Schmid ◽  
Ali Sheikhzadeh ◽  
Margareta Nordin ◽  
Jangwhon Yoon ◽  
...  
Keyword(s):  
Systematic Review ◽  
Skeletal Muscles ◽  
Morphological Changes ◽  
Fiber Type ◽  
Muscle Fibers ◽  
Type Ii ◽  
Review Of The Literature ◽  
Effects Of Aging ◽  
Complex Phenomena ◽  
Human Skeletal Muscles

The authors conducted a systematic review of the literature for scientific articles in selected databases to determine the effects of aging on Type II muscle fibers in human skeletal muscles. They found that aging of Type II muscle fibers is primarily associated with a loss of fibers and a decrease in fiber size. Morphological changes with increasing age particularly included Type II fiber grouping. There is conflicting evidence regarding the change of proportion of Type II fibers. Type II muscle fibers seem to play an important role in the aging process of human skeletal muscles. According to this literature review, loss of fibers, decrease in size, and fiber-type grouping represent major quantitative changes. Because the process of aging involves various complex phenomena such as fiber-type coexpression, however, it seems difficult to assign those changes solely to a specific fiber type.

scholarly journals DIAGNOSTIC VALUE OF MORPHOLOGICAL CHANGES IN GASTROESOPHAGEAL REFLUX DISEASE IN BIOPSY MATERIAL OF THE DISTAL ESOPHAGUS IN ADOLESCENTS SICKLY WITH ACUTE RESPIRATORY DISEASES

2019 ◽  
Vol 1 ◽  
pp. 15-23
Author(s):  
Olena Zhuravel ◽  
Tetyana Pochinok ◽  
Tamara Zadorozhna ◽  
Tetyana Archakova ◽  
Valentyna Zamula
Keyword(s):  
Gastroesophageal Reflux Disease ◽  
Gastroesophageal Reflux ◽  
Mucous Membrane ◽  
Reflux Disease ◽  
Respiratory Diseases ◽  
Morphological Changes ◽  
Diagnostic Value ◽  
Distal Esophagus ◽  
Biopsy Material ◽  
Acute Respiratory Diseases

The article dedicated to the problem of the diagnostic value of morphological changes in gastroesophageal reflux disease in the biopsy of the distal esophagus in pubertal children of childbearing age. Aim of the research is to investigate the diagnostic value of morphological changes in gastroesophageal reflux disease in esophageal biopsy material in adolescents sickly with acute respiratory diseases. Methodology. The objective of the study was achieved through examination of 90 adolescents (10 to 16 years old, average age 13.1±3.54 years) kept under observation at the Children’s Clinical Hospital No. 9 of Kyiv and on the basis of the Department of Pediatrics No. 1 Center of Primary Health Care No. 4 of the Desnianskyi district of Kyiv. All adolescents belonged to the group of sickly with a number of respiratory diseases averaging 6-8 times a year, lasting from 8 to 18 days (on average 12.8±5.41 days). All children have undergone endoscopic examination of the esophagus, stomach and duodenum with the esophagus mucosa biopsy using the OLYMPUS GIF-P3 flexible fiberscope. Results. It was found that the least valuable diagnostic feature in the morphological examination of the mucous membrane of the distal esophagus in the pain-causing children with GERD was thickening of the epithelium with a sensitivity of 13,0 %, a specificity of 96.0 %, and total value of 65.0 %. It has been proved that hyperplasia of cells of the basal layer of the mucous membrane of the distal esophagus at the GERD in the infected children is 46.7 % (specificity – 93.3 %, the total value is 75.6 %). Increase in the number of papillae and their prolongation in 33.3 % cases (sensitivity – 33.3 %, specificity – 93.3 %, overall diagnostic value – 70.8 %). Conclusion. The peculiarity of the morphological manifestations of GERD in childbearing children is dystrophic changes in keratocytes in the superficial parts of the multilayer squamous epithelium, which are detected at 100.0 % of patients (specificity is 93.3 %, total value is 96.8 %), with parakeratosis centers at 13.3 % of cases. It has been shown that a frequent and diagnostically valuable indication is inflammatory infiltration of the esophageal mucosa, which are verified in all cases (100.0 %, with dilatation and hyperemia in 46.7 % of patients (specificity – 40.0 %, total value – 81.3 %).

scholarly journals Comparative histochemical and morphometric analysis of muscle fibers of the psoas muscle in individuals of both genders with ageing

2020 ◽  
pp. 107-107
Author(s):  
Vladimir Antic ◽  
Nenad Stojiljkovic ◽  
Milorad Antic
Keyword(s):  
Morphometric Analysis ◽  
Statistical Significance ◽  
Muscle Fibers ◽  
Age Groups ◽  
Psoas Muscle ◽  
Iliopsoas Muscle ◽  
Type I ◽  
Type Ii ◽  
Tissue Samples ◽  
The Right

Background/Aim. In this paper, we analyzed type I and type II muscle fibers of the iliopsoas muscle in persons of both genders with ageing. The aim of this study was to detect the presence and distribution of types I and II muscle fibers in the human psoas muscle using the hematoxylin and eosin method in individuals of different ages and genders. Methods. The material consisted of tissue samples of the right iliopsoas muscle taken from 30 adult cadavers (18 males and 12 females), aged from 30 to 90 years, divided into three age groups. The material was obtained from the Institute of Forensic Medicine, Faculty of Medicine University of Nis. Results. The values of astereological parameters (area, perimeter and Feret's diameter) of type I and type II muscle fibers were higher in male cases, although without any statistical significance. Conclusion. Based on the histochemical and morphometric analysis, the conclusion was drawn that after 70 years of life there occurred a loss of type II muscle fibers, more conspicuous in female cases.

scholarly journals Evaluation of Cardiovascular Experiment Animal

2021 ◽  
Vol 9 (12) ◽  
pp. 2305-2310
Author(s):  
Kirtane Ramesh Kirtane
Keyword(s):  
Myocardial Infarction ◽  
Coronary Artery ◽  
Time Course ◽  
Muscle Fibers ◽  
Early Mortality ◽  
Ground Substance ◽  
Coronary Artery Ligation ◽  
Time Intervals ◽  
In Vivo Models ◽  
Immunohistochemical Studies

Abstract: In vivo models of myocardial infarction induced by coronary artery ligation in rats usually suffer from high early mortality and a low rate of induction. This study investigated the time course initiation of chronic myocardial infarction in albino rats and the possibility of reducing early mortality rate due to myocardial infarction by modification of the surgical technique. CAL was carried out by passing the suture through the pericardial layer around the midway of the left anterior descending coronary artery including a small area of the myocardium to avoid mechanical damage to the heart geometry. In addition, the role of endothelin-1 in rat heart with congestive heart failure was critically assessed. Time course initiation experiments were designed by sacrificing the animals at different time intervals and by carrying out physiological, biochemical, histopathological, electron microscopical and immunohistochemical studies. Specific markers of myocardial injury, viz. cardiac troponin-T, high sensitivity C-reactive protein, lactate dehydrogenase and fibrinogen were measured at different time points. Serum marker enzymes and activities of lysosomal hydrolases were found to be elevated on the eighth day post-ligation. Histopathological studies demonstrated focal areas showing fibrovascular tissue containing fibroblasts, collagenous ground substance and numerous small capillaries replacing cardiac muscle fibers. Transmission electron micrographs exhibited mitochondrial changes of well-developed irreversible cardiac injury, viz. swelling, disorganization of cristae, appearance of mitochondrial amorphous matrix densities, and significant distortion of muscle fibers and distinct disruption of the intercalated discs. Immune blotting studies confirmed the presence of alpha 2-macroglobulin which supported the inflammatory response. The severity of the CMI was inferred by the measurement of the level of ET-1 in plasma and left ventricle which was significantly higher in the CMI rats than in the sham-operated rats. Immunohistochemical studies at different time intervals showed that there was a significant immunoexpression of ET-1 on the eighth day post-ligation. This study conclusively showed that ligation of left anterior descending artery minimised mortality and ET-1 was expressed during CMI.

scholarly journals Toxicity and Teratogenic Effects of Zinc Sulfide Nanoparticles on Chick Embryo and Chick Fibroblast Cell Culture

2019 ◽  
Vol 25 (4) ◽  
pp. 270-281
Author(s):  
Asadollah Asadi ◽  
◽  
Arash Abdolmaleki ◽  
◽  
Keyword(s):  
Cell Culture ◽  
Zinc Sulfide ◽  
Morphological Changes ◽  
Lethal Dose ◽  
Fibroblast Cell ◽  
Zns Nanoparticles ◽  
Culture Methods ◽  
Study Results ◽  
Zinc Sulfide Nanoparticles ◽  
Fibroblastic Cells

Aims Nanoparticles (particles with a diameter of 10-500 nm) are currently used in the cosmetics industry as well as for pharmaceuticals, diagnostic imaging, and tissue engineering. Since these nanoparticles are used in industry and drug delivery, they can also be used by pregnant women. Thus, the current study investigated the teratogenic and cytotoxic effects of Zinc Sulfide (ZnS) nanoparticles on the embryo and their fibroblastic cell culture. Methods & Materials Zinc sulfide (ZnS) nanoparticles were synthesized. Then, nanoparticles at the concentrations of 5, 10, 15, 30, and 40 mg/mL/egg were injected into the air sac of the eggs in three replicates on the third day of incubation. Next, the treated and control eggs, on day 19 of incubation were opened, and embryos were weighted, and the relevant mortality rate was recorded. Fibroblast cells were isolated, cultured, and treated from the control embryo, and morphological changes and cell survival percentages were recorded. Findings The obtained results revealed that the embryos’ survival rate depends on the nanoparticle concentration. As a result, at the highest concentration, only 36.32% of the embryos survived, and the lethal dose 50% (LD50) was equal to 32.47 mg/egg. Morphological study of the treated embryos club foot and skeletal staining suggested the deletion of caudal vertebrate. The cytotoxicity study results of ZnS nanoparticles on fibroblastic cells indicated the survival fractions of 88.45%, 68.75%, and 49.32%, respectively, and its IC50 value was measured aas1460 μM. Conclusion The present study results suggested that ZnS nanoparticles had no significant toxic effects on the embryos and culture of chicken fibroblastic cells at low concentrations.

Alterations in intramuscular water movement associated with mechanical changes in human skeletal muscle fibers: an evaluation using magnetic resonance diffusion-weighted imaging and B-mode ultrasonography

2011 ◽  
Vol 52 (9) ◽  
pp. 1003-1008 ◽  
Author(s):  
Osamu Yanagisawa ◽  
Toshiyuki Kurihara ◽  
Toru Fukubayashi
Keyword(s):  
Magnetic Resonance ◽  
Ankle Joint ◽  
Structural Changes ◽  
Water Movement ◽  
Morphological Changes ◽  
Muscle Fibers ◽  
Medial Gastrocnemius ◽  
Plantar Flexors ◽  
Diffusion Weighted Images ◽  
Diffusion Weighted

Background Intramuscular water movement is expected to be affected by the mechanical changes of the muscle fibers. However, the effect of changes in fiber length (FL) and pennation angle (PA) on the water movement has not been sufficiently investigated in human skeletal muscles. Purpose To determine the relationship between intramuscular water movement and the mechanical changes in human muscle fibers. Material and Methods Axial magnetic resonance diffusion-weighted images of the right leg (eight men) were taken using a 1.5-Tesla device with the ankle joint maximally dorsiflexed and maximally plantar flexed. The apparent diffusion coefficient (ADC) values of both the dorsiflexors (the superficial and deep parts of the tibialis anterior) and the plantar flexors (medial gastrocnemius and soleus) were calculated along three orthogonal axes (S-I: superior-to-inferior, A-P: anterior-to-posterior, and R-L: right-to-left). FL and PA of both muscle groups were also calculated from longitudinal B-mode ultrasound images with the ankle joint maximally dorsiflexed and plantar flexed. Results There was a significant increase in the ADC in superficial ( P < 0.05) and deeP ( P < 0.05) parts of the dorsiflexors in the S-I direction when the ankle was plantar flexed and in the A-P and R-L directions when the ankle was dorsiflexed ( P < 0.05). The plantar flexors showed significantly elevated ADC in the S-I direction when the ankle was dorsiflexed ( P < 0.05), and in the A-P and R-L directions when the ankle was plantar flexed ( P < 0.05). The dorsiflexors also showed significantly increased PA and decreased FL values when the ankle was dorsiflexed ( P < 0.05). The plantar flexors displayed similar morphological changes when the ankle was plantar flexed ( P < 0.05). Conclusion Water diffusion is affected by structural changes in the long axis of the muscle fibers, namely the changes in PA and FL.

Chronic Peritoneal Inflammation by Cyanate in Rats

2000 ◽  
Vol 20 (6) ◽  
pp. 699-702 ◽  
Author(s):  
Kyo-Cheol Mun ◽  
Mi-Young Yeo ◽  
Sang-Pyo Kim ◽  
Hyun-Chul Kim ◽  
Chun-Sik Kwak
Keyword(s):  
Peritoneal Dialysis ◽  
Mononuclear Cells ◽  
Morphological Changes ◽  
Sodium Bicarbonate Solution ◽  
Mesothelial Cells ◽  
Control Group ◽  
Tissue Samples ◽  
Waste Products ◽  
Visceral Peritoneum ◽  
Standard Manner

Objective During peritoneal dialysis, the peritoneum is exposed to waste products, including urea. Urea forms cyanate spontaneously at body temperature and pH, and cyanate carbamylates amino acids, peptides, and proteins. Cyanate may contribute to peritoneal injury with morphological changes in the peritoneum. To test this hypothesis, we injected cyanate into rats. Methods Experiments were performed in two groups of 7 rats each. In the cyanate group, each rat received 1 mL of 1.5 μmol/L potassium cyanate dissolved in 40 mmol/L sodium bicarbonate solution intraperitoneally each experiment day. In the control group, each rat received 1 mL of 1.5 μmol/L potassium bicarbonate instead of potassium cyanate. The rats in both groups were anesthetized and killed at the 85th day after the first injection. After formalin fixation, tissue samples from abdominal walls and livers were sliced, embedded in a standard manner, and stained with hematoxylin and eosin. Results Parietal peritoneum from rats in the cyanate group showed a mild increase in the number of fibroblasts, with collagen deposits, infiltration by mononuclear cells, vascular congestion, round-shaped transformation of mesothelial cells, widening of submesothelial spaces, and abundant denudation of mesothelial cells. The visceral peritoneum from rats in the cyanate group showed collagen deposits with fibroblastic proliferation. Conclusions Cyanate can induce chronic inflammation in the peritoneum, and exposure of the peritoneum to cyanate may contribute to peritoneal injury in patients being treated with peritoneal dialysis.

scholarly journals Morphological changes in distant muscle fibers following thermal injury in Wistar rats

2010 ◽  
Vol 25 (6) ◽  
pp. 525-528 ◽  
Author(s):  
Flavia de Oliveira ◽  
Luci Rezende Bevilacqua ◽  
Carlos Alberto Anaruma ◽  
Silvia de Campos Boldrini ◽  
Edson Aparecido Liberti
Keyword(s):  
Body Surface ◽  
Wistar Rats ◽  
Thermal Injury ◽  
Morphological Changes ◽  
Muscle Fibers ◽  
The Body ◽  
Medial Gastrocnemius ◽  
Control Group ◽  
Hematoxylin And Eosin ◽  
Gastrocnemius Muscles

PURPOSE: Thermal injury causes catabolic processes as the body attempts to repair the damaged area. This study evaluated the effects of a scald injury on the morphology of muscle fibers belonging to a muscle distant from the lesion. METHODS: Thirty Wistar rats were divided into control (C) and scalded (S) groups. Group S was scalded over 45% of the body surface, standardized by body weight. Rats in both groups were euthanized at four, seven and 14 days following the injury. The middle portions of the medial gastrocnemius muscles were sectioned, stained with hematoxylin and eosin and Picrosirius, and submitted to histological analysis. RESULTS: Control group sections exhibited equidistantly distributed polygonal muscle fibers with peripheral nuclei, characteristic of normal muscle. The injured group sections did not consistently show these characteristics; many fibers in these sections exhibited a rounded contour, variable stain intensities, and greater interfiber distances. A substantially increased amount of connective tissue was also observed on the injured group sections. CONCLUSION: This experimental model found a morphological change in muscle distant from the site of thermal injury covering 45% of the body surface.

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