scholarly journals [Urethral discharge in Morocco: prevalence of microorganisms and susceptibility of gonococcos]

2002 ◽  
Vol 08 (06) ◽  
pp. 794-804
Author(s):  
K. Alami
Keyword(s):  
Sociodemographic Characteristics ◽  
Sex Partners ◽  
Chain Reaction ◽  
Protective Measures ◽  
Urethral Discharge ◽  
Multiple Sex Partners ◽  
History Of ◽  
Acute Form ◽  
The Mean ◽  
Polymerase Chain

We studied 422 patients with urethral discharge recruited from 4 sentinel sites in Morocco to determine sociodemographic characteristics, history of STI infection, infecting organism and antibiotic susceptibility of Neisseria gonorrhoeae. The mean age of the sample was 28 years [range 16-67 years], and most were single, had multiple sex partners without taking protective measures and came from all social backgrounds; 59.9% had a history of a previous STI. The majority [87%] of the infections were the acute form. By polymerase chain reaction of urine samples of 399 patients, 41.6% had N. gonorrhoeae infection, 6.3% Chlamidia trachomatis and 10.8% both organisms; in 41.4% no organism was identified. N. gonorrhoeae was strongly susceptible to ciprofloxacin.

scholarly journals MULTIPLEX POLYMERASE CHAIN REACTION (PCR) FOR THE DETECTION OF NEISSERIA GONORRHOEAE AND THE QUINOLONE RESISTANCE GENE IN PAKISTAN

2021 ◽  
Vol 71 (3) ◽  
pp. 866-69
Author(s):  
Hafsa Waseem ◽  
Afnan Naeem ◽  
Sakhawat Ali ◽  
Sharjeel Sarfraz ◽  
Javaid Usman ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Neisseria Gonorrhoeae ◽  
Multiplex Polymerase Chain Reaction ◽  
Simultaneous Detection ◽  
Antibiotic Use ◽  
Quinolone Resistance ◽  
Chain Reaction ◽  
Urethral Discharge ◽  
History Of ◽  
Polymerase Chain

Objective: To detect Neisseria gonorrhoeae from the urine of male patients reporting with active urethral discharge using multiplex polymerase chain reaction (PCR). And the simultaneous detection of the quinolone resistance determining region (QRDR) on the Neisseria gonorrhoeae gene using multiplex polymerase chain reaction. Study Design: Cross sectional study. Place and Duration of Study: Microbiology department, Army Medical College Rawalpindi Pakistan, from Mar to Dec 2018. Methodology: Male patients with active urethral discharge with no past history of antibiotic use for urethral discharge were included in study and patients without active urethral discharge and history of antibiotic use for urethral discharge were excluded. Urine of patients of active urethral discharge was collected and multiplex polymerase chain reaction was done by using two forward primers along with common reverse primer. Results: In this study 24 (40%) of patients who presented with active urethral discharge were positive for gonorrhea. However Quinolone Resistance Determining Region is detected in 17 (70.83%) of cases and only 7 (29.17%) were sensitive to ciprofloxacin. Conclusion: The multiplex polymerase chain reaction is very efficient and effective method for the simultaneous detection of Neisseria gonorrhoeae and status of isolate susceptibility to ciprofloxacin. And in Pakistan ciprofloxacin cannot be used as first line drug for the treatment of gonorrhea.

scholarly journals Seroprevalence of SARS-CoV-2 Antibodies Among Health Care Personnel at a Health Care System in Pakistan

2021 ◽  
pp. 101053952110110
Author(s):  
Salma Abbas ◽  
Aun Raza ◽  
Ayesha Iftikhar ◽  
Aamir Khan ◽  
Shahzaib Khan ◽  
...  
Keyword(s):  
Health Care ◽  
Control Measures ◽  
Health Care Personnel ◽  
Test Results ◽  
Antibody Testing ◽  
Chain Reaction ◽  
Past Infection ◽  
Infection Control Measures ◽  
History Of ◽  
Polymerase Chain

Health care personnel (HCP) are at high risk for coronavirus disease-2019 acquisition. Serum antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) indicate past infection. Our institution offered SARS-CoV-2 antibody testing to HCP. We surveyed HCP with positive test results to explore past exposure to SARS-CoV-2, details of symptoms during the preceding 6 months, and a history of SARS-CoV-2 polymerase chain reaction testing. A total of 2162 HCP underwent antibody testing. Eight hundred fifty-seven (39.6%) employees tested positive and, of these, 820 (95.7%) participated in the survey. When adjusted for age, males had higher odds of testing positive for SARS-CoV-2 antibodies compared with females (OR = 1.68; 95% CI = 1.37-2.05; P = .00) and clinical staff had higher odds of SARS-CoV-2 seropositivity compared with nonclinical staff (OR = 1.273; 95% CI = 1.06-1.53; P = .01). Implementation of effective infection control measures is essential to protect HCP from coronavirus disease-2019.

scholarly journals Application of a Polymerase Chain Reaction Assay for Detection of Proliferative Enteritis-Affected Swine Herds

1996 ◽  
Vol 8 (2) ◽  
pp. 181-185 ◽  
Author(s):  
Patricia K. Holyoake ◽  
Gary F. Jones ◽  
Peter R. Davies ◽  
Dennis L. Foss ◽  
Michael P. Murtaugh
Keyword(s):  
Polymerase Chain Reaction ◽  
Chain Reaction ◽  
Pcr Assay ◽  
Nursery Pigs ◽  
Clinically Normal ◽  
Source Of Infection ◽  
History Of ◽  
Polymerase Chain ◽  
Swine Herds ◽  
Age Range

A polymerase chain reaction (PCR) assay was used to confirm the presence of ileal symbiont (IS) intracellularis in 3 swine herds with a history of proliferative enteritis (PE). Two pooled fecal specimens, each comprising 5 individual stool samples, were collected from pen floors to screen for the presence of IS intracellularis and determine the age range of pigs shedding the organism. IS intracellularis was detected in the feces of clinically normal 10–25week-old grower/finisher pigs, indicating that this age range of pigs was the main source of infection for younger nursery pigs. Shedding continued without clinical disease when 10–100 g/ton of tylosin or 10 g/ton of chlortetracycline was added to the feed. PCR testing of pooled fecal samples can be used to identify groups of pigs affected with PE. The results of this study indicate that this PCR assay has the potential to accurately assess the IS intracellularis infection status of swine herds and the association of IS intracellular-is with PE and growth performance.

scholarly journals Xuezhikang Therapy Increases miR-33 Expression in Patients with Low HDL-C Levels

2014 ◽  
Vol 2014 ◽  
pp. 1-5 ◽  
Author(s):  
Ruihua Cao ◽  
Yongyi Bai ◽  
Lan Sun ◽  
Jin Zheng ◽  
Mian Zu ◽  
...  
Keyword(s):  
Hdl Cholesterol ◽  
Pcr Analysis ◽  
Chain Reaction ◽  
Low Hdl ◽  
The Mean ◽  
Polymerase Chain ◽  
Baseline Levels ◽  
Hdl Metabolism ◽  
To Receive ◽  
Plasma Mirnas

Background. MicroRNA-33a and -b (miR-33a/b) have been revealed to be posttranscriptional regulators of HDL metabolism. Xuezhikang (XZK) is a marked natural HDL-raising polypill. We aim to evaluate the effects of XZK on the expression of circulating miR-33a/b in patients with low plasma HDL-C levels.Methods. A total of 42 participating patients with low baseline levels of HDL cholesterol were assigned to receive an XZK capsule, 600 mg twice daily for 6 months. The expression of circulating miR-33a/b was detected at baseline and after XZK therapy measured with quantitative reverse-transcription (RT) polymerase chain reaction (PCR).Results. The mean (SD) HDL-C level after XZK treatment was 1.19 (0.13) mmol/L, representing an increase of 11.2% from baseline (P<0.001). Q-PCR analysis of plasma miRNAs revealed an increase in relative miR-33a/b expression with XZK treatment. The miR-33a expression was raised from 0.81 to 1.73 (P=0.012); miR-33b expression was increased from 1.2 to 2.75 (P<0.001). The changes of miR-33a and miR-33b were inversely related to the posttreatment LDL-C levels (r=-0.37,P=0.019;r=-0.33,P=0.035, resp.).Conclusion. In patients with low HDL-C levels, XZK therapy raised plasma levels of miR-33a and miR-33b, which may inhibit cellular cholesterol export and limit the HDL-raising effect of XZK.

scholarly journals Hoarseness of Voice as a Rare Presentation of Tuberculosis: A Case Report Study

2019 ◽  
Vol 7 (19) ◽  
pp. 3262-3264
Author(s):  
Taher Felemban ◽  
Abdullah Ashi ◽  
Abdullah Sindi ◽  
Mohannad Rajab ◽  
Zuhair Al Jehani
Keyword(s):  
Laryngeal Carcinoma ◽  
Incidental Finding ◽  
Chain Reaction ◽  
Rare Presentation ◽  
Case Presentation ◽  
X Ray ◽  
History Of ◽  
Chest X Ray ◽  
Polymerase Chain ◽  
Laryngeal Tuberculosis

BACKGROUND: Having hoarseness of voice as the first clinical manifestation of tuberculosis is rare. This atypical presentation causes some confusion since other more common conditions, such as laryngeal carcinoma, present similarly and might require more invasive tests to confirm the diagnosis. CASE PRESENTATION: A 38-year-old male presented to the otorhinolaryngology clinic with a four-month history of change in voice. Laryngoscopy demonstrated a right glottic mass, raising suspicion of laryngeal cancer. The computed tomography showed a mass and incidental finding of opacities in lung apices. Chest x-ray demonstrated findings suggestive of tuberculosis. Polymerase chain reaction and culture of sputum samples confirmed the diagnosis and the patient was started on anti-tuberculosis treatment. CONCLUSION: Despite accounting for only 1% of pulmonary tuberculosis cases and having a similar presentation to laryngeal carcinoma, we recommend considering laryngeal tuberculosis when evaluating hoarseness of voice in endemic areas.

scholarly journals Thrombotic, inflammatory, and HIF-regulated genes and thrombosis risk in polycythemia vera and essential thrombocythemia

2020 ◽  
Vol 4 (6) ◽  
pp. 1115-1130 ◽  
Author(s):  
Radhika Gangaraju ◽  
Jihyun Song ◽  
Soo Jin Kim ◽  
Tsewang Tashi ◽  
Brandi N. Reeves ◽  
...  
Keyword(s):  
Polycythemia Vera ◽  
Essential Thrombocythemia ◽  
Quantitative Polymerase Chain Reaction ◽  
Hypoxia Inducible Factor ◽  
Chain Reaction ◽  
Thrombosis Risk ◽  
Inflammatory State ◽  
History Of ◽  
Gene Transcripts ◽  
Polymerase Chain

Abstract Thrombosis is a major cause of morbidity and mortality in polycythemia vera (PV) and essential thrombocythemia (ET). The pathophysiology of thrombosis in these disorders remains unclear, and we hypothesized that upregulation of thrombotic, inflammatory, and hypoxia-inducible factor (HIF)–regulated genes may play a role in it. We performed unbiased RNA sequencing in granulocytes and platelets of PV patients and found differential expression of several thrombotic, inflammatory, and HIF-regulated genes. The expression of many of these genes positively correlated with JAK2 expression and JAK2V617F allelic burden. We then validated these findings by quantitative polymerase chain reaction analyses of selected gene transcripts in a larger number of PV and ET granulocytes and platelets (58 patients) and in 28 controls, and we compared these findings in patients with and without thrombosis. The study included 29 females and 29 males; of these, 28 had a history of thrombosis. We found that transcripts of several selected genes were upregulated in patients with PV or ET compared with controls. In granulocytes, the expression levels of F3, SELP, VEGFA, and SLC2A1 were significantly higher in patients with a history of thrombosis compared with those who did not have thrombosis. Patients with a history of thrombosis have significantly higher expression of IL1RAP (P &lt; .05) in platelets compared with those without thrombosis. Our study confirms the presence of a thrombo-inflammatory state and augmented HIF activity in PV and ET and its role in thrombosis. These data may provide the background for targeted therapies in PV and ET.

scholarly journals Epistaxis as a marker for severe acute respiratory syndrome coronavirus-2 status – a prospective study

2020 ◽  
Vol 134 (8) ◽  
pp. 717-720 ◽  
Author(s):  
MH Hussain ◽  
M Mair ◽  
P Rea
Keyword(s):  
Severe Acute Respiratory Syndrome ◽  
Prospective Study ◽  
Control Group ◽  
Study Group ◽  
Chain Reaction ◽  
Polymerase Chain Reaction Testing ◽  
The Mean ◽  
Polymerase Chain ◽  
The Difference ◽  
A Prospective Study

AbstractObjectiveTo evaluate the prevalence of severe acute respiratory syndrome coronavirus-2 infection in patients presenting with epistaxis to a tertiary otolaryngology unit.MethodsA prospective study was conducted of 40 consecutive patients presenting with epistaxis referred to our tertiary otolaryngology unit. A group of 40 age-matched controls were also included. All patients underwent real-time reverse transcriptase polymerase chain reaction testing for severe acute respiratory syndrome coronavirus-2. Symptoms of fever, cough and anosmia were noted in the study group.ResultsThe mean age was 66.5 ± 22.4 years in the study group. There were 22 males (55 per cent) and 18 females (45 per cent). The mean age in the control group was 66.3 ± 22.4 years (p = 0.935). There were six positive cases for severe acute respiratory syndrome coronavirus-2 (15 per cent) in the epistaxis group and one case (2.5 per cent) in the control group. The difference was statistically significant (p = 0.05).ConclusionEpistaxis may represent a presenting symptom of severe acute respiratory syndrome coronavirus-2 infection. This may serve as a useful additional criterion for screening patients.

scholarly journals Vulvar Tuberculosis—A Rare Manifestation of the Disease

2019 ◽  
Vol 41 (09) ◽  
pp. 575-578 ◽  
Author(s):  
Cristina Bragança ◽  
Inês Gonçalves ◽  
Luísa Guerreiro ◽  
Maria Janeiro
Keyword(s):  
Rare Case ◽  
Causes Of Death ◽  
World Health ◽  
Histological Findings ◽  
Chain Reaction ◽  
Rare Manifestation ◽  
The World ◽  
History Of ◽  
Polymerase Chain ◽  
Health Organization

AbstractTuberculosis is an infectious disease caused by Mycobacterium tuberculosis. According to data from the World Health Organization, this disease remains one of the leading causes of death worldwide. Although it most commonly affects the lungs, tuberculosis can compromise any organ. The present study reports a rare case of vulvar tuberculosis in a postmenopausal woman with a history of asymptomatic pulmonary and pleural tuberculosis, with no prior documented contact with the bacillus. Diagnosis was based on vulvar lesion biopsies, with histological findings suggestive of infection and isolation of M. tuberculosis by microbiological culture and polymerase chain reaction (PCR) essays. The lesions reverted to normal after tuberculostatic therapy.

scholarly journals COVID-19 Autopsies, Oklahoma, USA

2020 ◽  
Vol 153 (6) ◽  
pp. 725-733 ◽  
Author(s):  
Lisa M Barton ◽  
Eric J Duval ◽  
Edana Stroberg ◽  
Subha Ghosh ◽  
Sanjay Mukhopadhyay
Keyword(s):  
Diffuse Alveolar Damage ◽  
Nasopharyngeal Swab ◽  
Protective Equipment ◽  
Chain Reaction ◽  
Polymerase Chain Reaction Testing ◽  
Standard Procedures ◽  
History Of ◽  
Polymerase Chain ◽  
Viral Inclusions ◽  
Postmortem Examinations

Abstract Objectives To report the methods and findings of two complete autopsies of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) positive individuals who died in Oklahoma (United States) in March 2020. Methods Complete postmortem examinations were performed according to standard procedures in a negative-pressure autopsy suite/isolation room using personal protective equipment, including N95 masks, eye protection, and gowns. The diagnosis of coronavirus disease 2019 (COVID-19) was confirmed by real-time reverse transcriptase polymerase chain reaction testing on postmortem swabs. Results A 77-year-old obese man with a history of hypertension, splenectomy, and 6 days of fever and chills died while being transported for medical care. He tested positive for SARS-CoV-2 on postmortem nasopharyngeal and lung parenchymal swabs. Autopsy revealed diffuse alveolar damage and chronic inflammation and edema in the bronchial mucosa. A 42-year-old obese man with a history of myotonic dystrophy developed abdominal pain followed by fever, shortness of breath, and cough. Postmortem nasopharyngeal swab was positive for SARS-CoV-2; lung parenchymal swabs were negative. Autopsy showed acute bronchopneumonia with evidence of aspiration. Neither autopsy revealed viral inclusions, mucus plugging in airways, eosinophils, or myocarditis. Conclusions SARS-CoV-2 testing can be performed at autopsy. Autopsy findings such as diffuse alveolar damage and airway inflammation reflect true virus-related pathology; other findings represent superimposed or unrelated processes.

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