Uji aktivitas antijamur ekstrak black garlic terhadap pertumbuhan jamur Candida albicans

Salah satu tanaman yang berfungsi sebagai antijamur adalah bawang putih yang diinovasikan menjadi produk bawang yang berwarna hitam atau black garlic. Penelitian ini bertujuan untuk mengetahui aktivitas antijamur ekstrak black barlic terhadap pertumbuhan jamur Candida albicans. Metoda dalam penelitian ini adalah bawang putih dipanaskan selama 35 hari pada suhu 65°C untuk mendapatkan black garlic. Black garlic diekstraksi dengan metode maserasi menggunakan pelarut methanol. Uji aktivitas antibakteri berdasarkan uji zona hambat, konsentrasi hambat minimal (KHM) dan konsentrasi bunuh minimal (KBM). Uji zona hambat dengan variasi konsentrasi ekstrak 100%, 90% dan 80%. Konsentrasi dengan nilai zona hambat yang baik akan dilanjutkan ke uji KHM dan KBM dengan pengenceran ke 1 sampai 5. Hasil penelitian menunjukkan bahwa zona hambat ekstrak Black Garlic dengan konsentrasi 100% memiliki zona hambat paling bagus yaitu 3,15 mm. Uji KHM optimum diperoleh pada pengenceran ke-5 dengan nilai OD terendah 0,460. Uji KBM dari ekstrak black garlic pada pengenceran ke-5 menunjukkan koloni jamur C. albicans masih tumbuh, sehingga dapat dikatakan bahwa ekstrak black garlic hanya mampu menghambat pertumbuhan jamur namun tidak bisa membunuh jamur C. albicans. Kata kunci: antijamur; black garlic; maserasi ABSTRACTActivity test of black garlic extract against the growth of the fungi Candida albicans. One plant that functions as an antifungal is garlic which is innovated into black garlic. The aim of this study is to indentify black garlic extract antifungal activity against of the Candida albicans. The method in this research was heated garlic for 35 days at temperature of 65°C. Black garlic was extracted by maceration method using methanol solvent. Antibacterial activity test based on inhibition zone test, minimal inhibitory concentration (MIC) and minimal kill concentration (MBC). Inhibition zone test with various extract concentrations of 100%, 90% and 80%. Concentrations with good inhibition zone values will be continued to the MIC and MBC tests with dilutions 1 to 5. The results showed that the inhibition zone of black garlic extract with a concentration of 100%, 3.15 mm. The optimum MIC test was obtained at the 5 dilution with the lowest OD value of absorbance 0.460. The MBC test of the black garlic extract at the 5 dilution showed C. albicans fungal colonies were still growing, so it can be said that the black garlic extract was only able to inhibit fungal growth but could not kill C. albicans fungi. Keywords: antifungal; black garlic; maceration

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Antifungal activity of onion (Allium cepa L.) essential oil on Candida albicans

Ilmu Gizi Indonesia ◽

10.35842/ilgi.v3i1.128 ◽

2019 ◽

Vol 3 (1) ◽

pp. 59

Author(s):

Miftahur Rahmi ◽

Felicia Aileen Agustia

Keyword(s):

Candida Albicans ◽

Minimum Inhibitory Concentration ◽

Antifungal Activity ◽

Essential Oil ◽

Allium Cepa ◽

Inhibitory Concentration ◽

Fungal Growth ◽

Diffusion Method ◽

Minimum Concentration ◽

Allium Cepa L

Background:Onions (Allium cepa L.) contain essential oil that have sulfur compounds called thiosulfinates or isothiocyanates which are known to have antibacterial and antifungal effects. Minimum Inhibition Concentration (MIC) test is needed to determine the minimum concentration of onion essential oil which can inhibit or kill fungus test. Objectives: To determine the antifungal activity of essential oil in onion (Allium cepa L.) and to find out the Minimum Inhibitory Concentration (MIC) on Candida albicans. Methods:This research was an experimental study with disc diffusion method (Kirby Bauer) using Sabourond Dextrose Agar (SDA) media. Results: The results were obtained at concentrations of 20% (C1); 10% (C2); 5% (C3); 2.5% (C4); 1.25% (C5); 0.625% (C6); and 0.312% (C7) provided inhibition with an average diameter of 13.14 mm; 9.33 mm; 8.49 mm; 6.90 mm; 0 mm; 0 mm; and 0 mm respectively. Conclusion: Essential oil of onion (Allium cepa L.) could inhibit fungal growth of Candida albicans and Minimum Inhibitory Concentration (MIC) of essential oil onions (Allium cepa L.) indicated on concentration of 2.5% with a flat diameter average of 6.90 mm.

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Antifungal activity of plant-based tinctures on Candida

RSBO ◽

10.21726/rsbo.v9i1.961 ◽

2013 ◽

Vol 9 (1) ◽

pp. 25-30

Author(s):

Andreia Medeiros Rodrigues Cardoso ◽

Yuri Wanderley Cavalcanti ◽

Leopoldina de Fátima Dantas de Almeida ◽

Ana Luíza Alves de Lima Pérez ◽

Wilton Wilney Nascimento Padilha

Keyword(s):

Candida Albicans ◽

Statistical Analysis ◽

Minimum Inhibitory Concentration ◽

Antifungal Activity ◽

Inhibitory Concentration ◽

Fungal Growth ◽

Initial Concentration ◽

Malva Sylvestris ◽

Fungal Inhibition

To evaluate through determination of minimum inhibitory concentration (MIC) the antifungal activity of Salvia officinalis (sage), Anacardium occidentale (cashew) and Malva sylvestris (mallow) tinctures on Candida albicans (ATCC 40227), C. tropicalis (ATCC 13803) and C. krusei (ATCC 40147). Material and methods: In 96-well microplates, 100 µl of Sabouraud-Dextrose broth doubly concentrated, 100 µl of the tested tinctures and 10 µl of fungal inoculums (1.5 x 106organisms/ml) were inserted. The products were diluted from initial concentration of 100 mg/ml until 0.78 mg/ml. MIC corresponded to the lowest dilution at which there was no visible fungal growth. Nystatin (100,000 UI/ml) was used as control. Statistical analysis was performed by Kruskal-Wallis and Dunn tests (p < 0.05). Results: S. officinalis tincture did not inhibit the growth of C. albicans and C. tropicalis; MIC was 100 mg/ml for C. krusei. For A. occidentale, MIC was 100 mg/ml for C. albicans and C. krusei, and for C. tropicalis, there was no fungal inhibition. M. sylvestris tincture presented MIC at 25 mg/ml for C. krusei and 100 mg/ml for C. albicans and C. tropicalis. The best antifungal activity was showed by M. sylvestris tincture (p < 0.05). Conclusion: M. sylvestris tincture exhibited antifungal activity against all the tested strains at lower concentrations. S. officinalis tincture inhibited the action of C. krusei and A. occidentale tincture showed activity against C. albicans and C. tropicalis.

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Antioxidant and Antifungal Activity of Jeringau (Acorus calamus L.) Extract In Some Organic Solvents In Vitro

el–Hayah ◽

10.18860/elha.v6i3.5334 ◽

2017 ◽

Vol 6 (3) ◽

pp. 70

Author(s):

Bayyinatul Muchtaromah ◽

Mujahidin Ahmad ◽

M. Nur Hasan ◽

Didik Wahyudi

Keyword(s):

Candida Albicans ◽

Antifungal Activity ◽

Organic Solvents ◽

Inhibition Zone ◽

Acorus Calamus ◽

Plate Method ◽

Activity Test ◽

Dpph Method ◽

Mic Value

Candidiasis is one disease that has experienced by 75% of women in Indonesia caused by fungal infection,Candida albicans. Candidiasis can cause serious complications such as infertility. One of the best known ingredients to cure infertility problems is jeringau rhizome (Acoruscalamus L.). This research is the first step to process for <ins datetime="2018-05-29T04:08"> </ins>standardization and scientification of jeringau rizhome as one of the basic ingredients of Madura traditional medicine, jamu "SuburKandungan". It was aimed to investigate antioxidant and antifungal activity of jeringau rizhome in some organic solvents. Samples were extracted by maceration method using ethanol, chloroform, and n-hexane. Antioxidant activityassaywas determined using DPPH method.Ascorbic acid was used as control. Antifungal activity test on Candida albicans was done by usingkirby bauer method to measure diameter of inhibition zone and microdilution plate method to determine MIC and MFC. The highest antioxidant activity was revealed by ethanolfollowed by chloroform and n-hexane, while the highest antifungal activity was obtained by ethanol followed by n-hexane and chloroform. The MIC value of ethanol, chloroform and n-hexanewere founded at concentration of 0.39% and the MFC at a concentration of 0.78%.

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Complexes of Cu (II) with a-Ketoglutaric Acid and 1- (o-tolyl) Biguanide Synthesis, characterization and biological Activity

Revista de Chimie ◽

10.37358/rc.19.10.7605 ◽

2019 ◽

Vol 70 (10) ◽

pp. 3603-3610

Author(s):

Madalina Mihalache ◽

Cornelia Guran ◽

Aurelia Meghea ◽

Vasile Bercu ◽

Ludmila Motelica ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Antimicrobial Activity ◽

Antibacterial Activity ◽

Biological Activity ◽

Candida Albicans ◽

Antifungal Activity ◽

Copper Complexes ◽

Substrate Adhesion ◽

Inert Substrate

The three copper complexes having a-ketoglutaric acid (H2A) and 1- (o-tolyl) biguanide (TB) ligands have been synthesized and characterized. The proposed formulas for these complexes are: [Cu(TB)(HA)]Cl (C1), [Cu(TB)(HA)CH3COO]�H2O (C2) and [Cu(TB)(HA)](NO3) (C3) where HA represents deprotonated H2A. The complexes obtained were tested for antibacterial activity against Staphylococcus aureus ATCC 25923 and Pseudomonas aeruginosa ATCC 27853, antifungal activity on Candida albicans ATCC 10231 and antitumor activity on HeLa tumor cells. Due to the antitumor, antifungal, antimicrobial activity and inhibition of inert substrate adhesion, complexes synthesized could be used for potential therapeutic applications.

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Antifungal activity of components used for decontamination of dental prostheses on the growth of Candida albicans

Revista de Odontologia da UNESP ◽

10.1590/rou.2014.018 ◽

2014 ◽

Vol 43 (2) ◽

pp. 137-142 ◽

Cited By ~ 2

Author(s):

Cíntia Lima Gouveia ◽

Isabelle Cristine Melo Freire ◽

Maria Luísa de Alencar e Silva Leite ◽

Rebeca Dantas Alves Figueiredo ◽

Leopoldina de Fátima Dantas de Almeida ◽

...

Keyword(s):

Candida Albicans ◽

Antifungal Activity ◽

Sodium Bicarbonate ◽

Fungal Growth ◽

Antifungal Effect ◽

Dental Prostheses ◽

Diffusion Technique ◽

Zones Of Inhibition ◽

And Control

Introduction: The effectiveness of antimicrobial solutions employed in dental prosthesis decontamination is still uncertain. Aim: To evaluate the antifungal activity of cleaners used in the decontamination of dental prostheses on the growth of Candida albicans. Material and method: The evaluated products were: Corega Tabs(r) (S1), Sodium Hypochlorite 1% (S2), Sodium Bicarbonate 1% (S3), Hydrogen Peroxide 1% (S4), Chlorhexidine Digluconate 0.12% - Periogard (r) (S5), Mouthrinse based on essential oils - Listerine(r) (S6), essential oil from Rosmarinus officinalis (rosemary) at concentrations of 1% (S7) and 2% (S8). The antifungal activity of the products was evaluated by agar diffusion technique and the determination of microbial death curve of samples of C. albicans (ATCC 90028) in concentration 1.5 × 106 CFU/mL. The tests were performed in triplicate and statistical analysis was made by ANOVA Two-Way and Tukey tests, with the confidence level of 95%. Result: The average of the zones of inhibition growth, in millimeters, obtained for the products were: 0.0 (S1), 44.7 (S2), 0.0 (S3), 21.6 (S4), 10.0 (S5), 6.1 (S6), 0.0 (S7) and 2.4 (S8). Considering the determination of microbial death curve, all products showed a statistical difference (p<0.01) from control (0.85% sodium chloride) and S3 groups. Fungal growth less than 2×104 CFU/mL and an accentuation of the microbial death curve were observed after 30 minutes, with exception for S3 and control groups. Conclusion: The studied compounds, with the exception of Sodium Bicarbonate, have antifungal effect against C. albicans, which contribute for dental prostheses hygiene.

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ANTI-CANDIDA ALBICANS ACTIVITY OF THE ASSOCIATION OF CITRONELAL WITH ANFOTERICIN B OR WITH CETOCONAZOLE

Periódico Tchê Química ◽

10.52571/ptq.v16.n31.2019.256_periodico31_pgs_250_257.pdf ◽

2019 ◽

Vol 16 (31) ◽

pp. 250-257

Author(s):

Patrícia Duarte Costa SILVA ◽

Brenda Lavínia Calixto dos SANTOS ◽

Gustavo Lima SOARES ◽

Wylly Araújo de OLIVEIRA

Keyword(s):

Candida Albicans ◽

Antifungal Activity ◽

Amphotericin B ◽

Inhibitory Concentration ◽

Fungal Infections ◽

Pathogenic Fungi ◽

Mortality Rates ◽

New Drugs ◽

High Morbidity ◽

Isolated Species

Fungal infections caused by species of the genus Candida are responsible for high morbidity and mortality rates, mainly affecting immunocompromised individuals. Among fungi, Candida albicans is the most frequently isolated species of clinical specimens. A problem associated with increased resistance of pathogenic fungi to the agents used in the therapeutic regimen and the limited number of drugs to cure these infections. As a result, the search for new drugs with antifungal activity has become increasingly important. The aim of this study is to study the antifungal activity of citronellal alone and in combination with amphotericin B or ketoconazole. The Minimal Inhibitory Concentration of citronellal, amphotericin B and ketoconazole against strains of Candida albicans were evaluated by the microdilution technique, and the Minimum Fungicide Concentration of citronellal against the same strains was also performed. Through the checkerboard methodology the effect of the combination of citronelal with amphotericin B or with ketoconazole was determined. This study showed that the association of citronellal with ketoconazole was shown to be an additive against one of the strains of C. albicans and indifferent to another strain. While the combined activity of citronellal and amphotericin B demonstrated an indifferent effect on the strains tested.

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ANTIBACTERIAL AND ANTIFUNGAL ACTIVITY OF SILVER NANOPARTICLES AGAINST NEISSERIA GONORRHOEAE AND CANDIDA ALBICANS

International Journal of Research -GRANTHAALAYAH ◽

10.29121/granthaalayah.v8.i6.2020.461 ◽

2020 ◽

Vol 8 (6) ◽

pp. 179-187

Author(s):

Titik Taufikurohmah ◽

Tasha Anandya Tantyani

Keyword(s):

Antibacterial Activity ◽

Candida Albicans ◽

Antifungal Activity ◽

Neisseria Gonorrhoeae ◽

Diffusion Method ◽

Antibacterial And Antifungal Activity ◽

Disk Diffusion Method ◽

Fungal Inhibition ◽

Inhibition Response ◽

Antibacterial And Antifungal

This Research on the antibacterial and antifungal activity of nanosilver against Neisseria gonorrhoeae and Candida albincas fungi has been carried out. The purpose of this study was to determine antibacterial activity of nanosilver against Neisseria gonorrhoeae and antifungal activity against Candida albincas. Synthesis Nanosilver uses bottom up method and characterized using UV-Vis Spectrophotometer. Nanosliver concentrations used were 30, 40, 50, and 60 ppm. Antibacterial and antifungal activity tests using disk diffusion method. Observations obtained in form of the presence or absence of clear zones formed around paper discs indicate the inhibition of nanosilver on microbial growth. The results of testing the antifungal activity of Candida albicans on nanosilver with concentrations of 30, 40, 50 and 60 ppm resulted in clear zones of 9.73 nm, 11.46 nm, 11.93 nm, and 13 nm with fungal inhibition response categories is medium and strong. The results antibacterial activity test of Neisseria gonorrhoeae on nanosilver with concentrations of 30, 40, 50 and 60 ppm did not show any clear zone around the disc, it showed that nanosilver in this study did not have antibacterial activity against Neisseria gonorrhoeae.

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Antibacterial Activity Test of Bacteriocin from Lactobacillus brevis, Lactobacillus casei and Lactobacillus plantarum Against Gram Positive Pathogenic Bacteria

Journal of Tropical Biodiversity and Biotechnology ◽

10.22146/jtbb.38138 ◽

2018 ◽

Vol 3 (3) ◽

pp. 85

Author(s):

Novi Permata Sari ◽

Rafika Sari ◽

Eka Kartika Untari

Keyword(s):

Antibacterial Activity ◽

Lactobacillus Plantarum ◽

Lactobacillus Casei ◽

Pathogenic Bacteria ◽

Heating Temperature ◽

Lactobacillus Brevis ◽

Inhibition Zone ◽

Diffusion Method ◽

Activity Test ◽

Ph Range

Bacteriocin is a secondary metabolite product of lactic acid bacteria (LAB) which have an antimicrobial and potentially as a natural preservative. LAB isolates used in this study were Lactobacillus brevis, Lactobacillus casei and Lactobacillus plantarum. This study aimed to determine the antibacterial activity of bacteriocin produced by each isolate of LAB including the influence of pH and heating variation against Bacillus cereus, Bacillus subtilis and Staphylococcus epidermidis. Antibacterial activity test was done by using disc diffusion method. method. Confirmation test using proteolytic enzyme aimed to analyse that the inhibition zone produced from the activity of bacteriocin. The inhibition zone produced from L. brevis, L. casei and L. plantarum against B. cereus were 15.70, 16.43 and 14.50 mm, against B. subtilis were 13.37, 14.10 and 12.53 mm and against S. epidermidis were 11.37, 14.50 and 12.45 mm. The activity of each bacteriocin decreased with the addition of trypsin and catalase, bacteriocin was active in the pH range of 2-10 and heating temperature of 40-121oC. Statistical test showed that the addition of trypsin, catalase and the variation of pH also heating had significant differences (p<0.05) to antibacterial activity produced by bacteriocin from L. brevis, L. casei and L. plantarum.

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Antimicrobial Activity of N,N'-bis(decylmethyl)- α,ω-alkanediamine Dioxides [1]

Zeitschrift für Naturforschung C ◽

10.1515/znc-1983-1-227 ◽

1983 ◽

Vol 38 (1-2) ◽

pp. 151-152 ◽

Cited By ~ 1

Author(s):

Ferdinand Devínsky ◽

Ivan Lacko ◽

Ludovít Krasnec ◽

Dušan Mlynarčík

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antimicrobial Activity ◽

Candida Albicans ◽

Minimal Inhibitory Concentration ◽

Inhibitory Concentration

Antimicrobial activity of N,N′-bis(decylmethyl)-α,ω-alkanediamine dioxides determined on Staphylococcus aureus, Escherichia coli, and Candida albicans is presented as minimal inhibitory concentration (MIC). The effect of the length of linking alkylene chain on this activity has been followed.

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Synthesis and Antimicrobial Screening of Some Novel 5-Substituted-1,10b-dihydroimidazole[1,2-c]quinazoline Derivatives

International Journal of Pharmaceutical Sciences and Nanotechnology ◽

10.37285/ijpsn.2021.14.2.6 ◽

2021 ◽

Vol 14 (2) ◽

pp. 5406-5411

Author(s):

Haribhai Rabari ◽

Hetal Vankar ◽

Beenkumar Prajapati

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Candida Albicans ◽

Antifungal Activity ◽

Aspergillus Niger ◽

Antifungal Compound ◽

Bacterial Strains ◽

Standard Drug ◽

Quinazoline Derivatives

The emergence of multidrug microbial resistance is the main challenges that the modern scientists have so far been facing in the recent era. In this respect, new series of drug classes having potential to give antimicrobial effect have been synthesized. A new series of 5- substituted-1,10 b-dihydroimidazole[1,2-c]quinazoline derivatives 8a-e have been synthesized and screened for antibacterial activity and antifungal activity. Synthesized derivatives were characterized by IR, MASS and 1H-NMR spectroscopy. Synthesized compounds show good activity, which was comparable to the standard drug and it can be useful for the further clinical study. Antibacterial activity was evaluated against four different pathogenic bacterial strains like Staphylococcus aureus, Enterococcus faecalis, Escherichia coli and Pseudo-monas aeruginosa. Among the screened compounds, 8e show good antibacterial activity against Staphylococcus aureus and Escherichia coli with MIC of 50 and 100 μg/ml respectively. Antifungal activity was evaluated against two strains of fungi. Among the synthesized derivates, compound 8c was emerged out as the potent antifungal compound against Candida albicans and Aspergillus niger with MIC of 25 μg/ml and 75μg/ml respectively. Compound 8e also shows good antifungal activity with MIC of 50 μg/ml against both Candida albicans and Aspergillus niger. The overall results of this study indicated that synthesized quinazoline derivatives had the potential to act as an antibacterial and antifungal agent, hence further investigation is warranted.

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