scholarly journals Antibacterial Activity Test of Bacteriocin from Lactobacillus brevis, Lactobacillus casei and Lactobacillus plantarum Against Gram Positive Pathogenic Bacteria

2018 ◽  
Vol 3 (3) ◽  
pp. 85
Author(s):  
Novi Permata Sari ◽  
Rafika Sari ◽  
Eka Kartika Untari
Keyword(s):  
Antibacterial Activity ◽  
Lactobacillus Plantarum ◽  
Lactobacillus Casei ◽  
Pathogenic Bacteria ◽  
Heating Temperature ◽  
Lactobacillus Brevis ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Activity Test ◽  
Ph Range

Bacteriocin is a secondary metabolite product of lactic acid bacteria (LAB) which have an antimicrobial and potentially as a natural preservative. LAB isolates used in this study were Lactobacillus brevis, Lactobacillus casei and Lactobacillus plantarum. This study aimed to determine the antibacterial activity of bacteriocin produced by each isolate of LAB including the influence of pH and heating variation against Bacillus cereus, Bacillus subtilis and Staphylococcus epidermidis. Antibacterial activity test was done by using disc diffusion method. method. Confirmation test using proteolytic enzyme aimed to analyse that the inhibition zone produced from the activity of bacteriocin. The inhibition zone produced from L. brevis, L. casei and L. plantarum against B. cereus were 15.70, 16.43 and 14.50 mm, against B. subtilis were 13.37, 14.10 and 12.53 mm and against S. epidermidis were 11.37, 14.50 and 12.45 mm. The activity of each bacteriocin decreased with the addition of trypsin and catalase, bacteriocin was active in the pH range of 2-10 and heating temperature of 40-121oC. Statistical test showed that the addition of trypsin, catalase and the variation of pH also heating had significant differences (p<0.05) to antibacterial activity produced by bacteriocin from L. brevis, L. casei and L. plantarum. 

scholarly journals Eksplorasi Antibakteri dari Kapang Tanah Arboretum

2020 ◽  
Vol 25 (3) ◽  
pp. 456-461
Author(s):  
Rizal Dwinto Rochman ◽  
Titiek Sunartatie ◽  
Usamah Afiff
Keyword(s):  
Antibacterial Activity ◽  
Antibacterial Agent ◽  
Pathogenic Bacteria ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Mold Growth ◽  
Antibiotic Resistant ◽  
Coli Isolate ◽  
Activity Test ◽  
Agar Well Diffusion Method

Research to discover new antibacterial agent has been increasing due to the increasing number of pathogenic bacteria become antibiotic-resistant. Microbiomes in soil mold have antibacterial potency by producing secondary metabolites. The aim of this research was to isolate soil molds from the Faculty of Forestry Arboretum at IPB University. Arboretum’s soil was sampled ± 7.5 g from several points with maximum depth of 15 cm from surface. Mold isolate were cultivated for 28 days using waterbath shaker. Cultivated mycelium and soil filtrate were then separated and extracted using different methods and solvents, prior to antibacterial activity test using agar well diffusion method. Six isolates from Aspergillus, Penicillium, Paecilomyces, and Verticilium were used in this research. Cultivatation using sabouraud dextrose broth (SDB) contains pepton and dextrose in 25°C capable of stimulating mold growth. The research showed the result of secondary metabolit had antibacterial characteristic toward tested bacteria. The results of antibacterial activity test on both mycelium and soil filtrate extract showed that soil mold isolates had antibacterial activity against Staphylococcus aureus and Escherichia coli. Isolate 103A originated from soil filtrate formed the largest inhibition zone on both tested pathogens.   Keywords: antibacteria, mycelium, soil filtrate, soil mold

scholarly journals AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL BIJI KAPULAGA JAWA (Amomum compactum Soland. ex Maton) TERHADAP Streptococcus pyogenes

EKOLOGIA ◽  
2020 ◽  
Vol 20 (1) ◽  
pp. 31-39
Author(s):  
Oom Komala ◽  
. Ismanto ◽  
Muhammad Alan Maulana
Keyword(s):  
Antibacterial Activity ◽  
Streptococcus Pyogenes ◽  
Pathogenic Bacteria ◽  
Inhibition Zone ◽  
Positive Control ◽  
Negative Control ◽  
Diffusion Method ◽  
Dilution Method ◽  
Natural Treatment ◽  
Amomum Compactum

Streptococcus pyogenes is one of the pathogenic bacteria that causes pharyngitis. Natural treatment to overcome these problems is to use cardamom seeds. The purpose of this study was to test the antibacterial activity, determine the concentration of inhibitory zone and phytochemical compounds from  ethanol 96% extract of Java cardamom seeds (Amomum compactum Soland. Ex Maton) against Streptococcus pyogenes. The method is used   solid dilution and paper disc diffusion method. The solid dilution method is used for the Minimum Inhibitory Concentration (MIC) test with a concentration of 1.25%, 2.5%, 5% and 7.5% while the paper diffusion method is used for the Inhibition zone Diameter (IZD)  using five treatments namely three concentrations of ethanol 96% extract of Java cardamom seeds (7.5%, 10% and 12%), one positive control of amoxicillin 0.01 mg/mL and one negative control of sterile distilled water. IZD data were  analyzed using ANOVA with a confidence level of 95% and α = 0.05 and Duncan's further tests to determine differences between treatments. The results showed that the MIC  was at a concentration of 7.5% while for the IZD test which had the highest activity there was a concentration of 12% with an average inhibition diameter of 12.03 ± 0.14 mm. In addition, ethanol 96% extract of Java cardamom seeds contain alkaloids, flavonoids, terpenoids and tannins which function in antibacterial activity.

scholarly journals Combined activity of Azadirachta indica and Dodonea viscose leaves extract on some selected pathogens

2020 ◽  
Vol 12 (1) ◽  
pp. 31-38
Author(s):  
A. I Isah ◽  
A.I. Aminu
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Petroleum Ether ◽  
Azadirachta Indica ◽  
Pathogenic Bacteria ◽  
Bacterial Species ◽  
Plant Secondary Metabolites ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Plant Materials

The study was undertaken to investigate the antimicrobial activity both individually and in combination of two Nigerian plants (Azadirachta indica and Dodonea viscosa) against a variety of pathogenic bacteria. Five bacterial species namely; Staphylococcus aureus, Escherichia coli, Salmonella typhi, Klebsiella pneumoniae and Pseudomonas aeruginosa were selected for the assays. The powdered plant materials of the two plants were extracted using sohxlet extraction technique with methanol water and petroleum ether as solvents. The crude extracts of the two plants were subjected to phytochemical screening for qualitative detection of plant secondary metabolites. The extracts were further tested for antibacterial activity against the selected pathogens singly and then combined using agar well diffusion method. Antibacterial activity of the two plants singly indicates that the methanolic extract possess the highest antibacterial activity at a concentration of 50mg/ml with an inhibition zone of 23.3±0.5mm compared to aqueous extract with 21.7±0.5mm inhibition zone at a concentration of 50mg/ml. The least activity was observed with the petroleum ether extract with an inhibition zone of 7.3±0.5mm at a concentration of 12.5mg/ml. Combination of the plants extracts exhibited lower antibacterial activity on the test isolates compared to single plants as evidenced by the production of lower inhibition zones. Key words: Azadirachta indica, Dodonea viscosa, pathogens, antimicrobial activity, synergistic effect

scholarly journals UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL BAWANG DAYAK (Eleutherine americana Merr) TERHADAP BAKTERI Staphylococcus aureus, Escherichia coli DAN Salmonella typhi

PHARMACON ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 525
Author(s):  
Rafi’a Adinda Putri ◽  
Herny E.I. Simbala ◽  
Deby A. Mpila
Keyword(s):  
Antibacterial Activity ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Salmonella Typhi ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
E Coli ◽  
Activity Test ◽  
Paper Disk ◽  
Eleutherine Americana

ABSTRACTDayak onions (Eleutherine americana Merr) has been used as a traditional plant which has  benefits  for  many  diseases,  one  of  them  is  antibacterial. This study aims to determine the growth inhibition of ethanol extract of Dayak onions leaves on the bacterium S. aureus, E. coli and  S. typhi using three concentrations of 20%, 40% and 60%. Extraction was done by maceration method using ethanol 96%. Antibacterial activity test was performing using the paper disk diffusion method (Kirby-Bauer diffusion). The results showed that ethanol extract of bawang dayak has the potential as an antibacterial and has antibacterial strength against S. aureus, E. coli and S. typhi. At concentrations of 20%, 40% and 60% forming inhibition zone diameters (16.23 mm, 19.18 mm, 21.25 mm) S. aureus, (10.7 mm, 13.98 mm, 15.87 mm) E. coli, and  (14.43 mm, 14.6 mm, 17.2 mm) S. typhi. At concentration of 20%, 40% and 60% included strong groups to inhibit E. coli and  S. typhi, while at concentration of 60%, it is included in a very strong group to inhibit the S. aureus bacteria. Keywords: Antibacterial activity, Bawang Dayak , Kirby-Bauer diffusion method   ABSTRAK Bawang Dayak (Eleutherine americana Merr) telah digunakan sebagai tanaman tradisional yang bermanfaat bagi banyak penyakit salah satunya sebagai antibakteri. Penelitian ini bertujuan untuk mengetahui ekstrak etanol bawang dayak terhadap bakteri S. aureus, E.coli dan S. typhi menggunakan tiga kosentrasi yaitu 20%, 40% dan 60%. Ekstraksi dilakukan dengan metode maserasi menggunakan etanol 96%. Pengujian aktivitas antibakteri dilakukan dengan  menggunkan metode difusi dengan cakram kertas (difusi Kirby-Bauer). Hasil penelitian menunjukkan bahwa ekstrak etanol bawang dayak berpotensi sebagai antibakteri dan memiliki kekuatan antibakteri terhadap S. aureus, E. coli dan S. typhi. Pada konsentrasi 20%, 40% dan 60% membentuk diameter zona hambat (16.23 mm, 19.18 mm, 21.25 mm) pada S. aureus, (10.7 mm, 13.98 mm, 15,87 mm) pada E. coli, (14.43 mm, 14.6 mm, 17.2 mm) pada S. typhi. Pada konsentrasi 20%, 40% dan 60% termasuk golongan kuat untuk menghambat bakteri E. coli dan S. typhi, sedangkan pada konsentrasi 60% termasuk dalam golongan sangat kuat untuk menghambat bakteri S. aureus. Kata kunci: Aktivitas antibakteri, Bawang Dayak, metode difusi Kirby-Bauer

scholarly journals Chemical Composition and Antibacterial Activity of Essential Oils ofTagetes minuta(Asteraceae) against Selected Plant Pathogenic Bacteria

2016 ◽  
Vol 2016 ◽  
pp. 1-9 ◽  
Author(s):  
Martin Muthee Gakuubi ◽  
John M. Wagacha ◽  
Saifuddin F. Dossaji ◽  
Wycliffe Wanzala
Keyword(s):  
Antibacterial Activity ◽  
Chemical Composition ◽  
Essential Oils ◽  
Pathogenic Bacteria ◽  
Inhibition Zone ◽  
Antibacterial Activities ◽  
Scientific Basis ◽  
Diffusion Method ◽  
Phytopathogenic Bacteria ◽  
Xanthomonas Axonopodis

The objective of this study was to determine the chemical composition and antibacterial activity of essential oils (EOs) ofTagetes minutaagainst three phytopathogenic bacteriaPseudomonas savastanoipv.phaseolicola,Xanthomonas axonopodispv.phaseoli, andXanthomonas axonopodispv.manihotis. The essential oils were extracted using steam distillation method in a modified Clevenger-type apparatus while antibacterial activity of the EOs was evaluated by disc diffusion method. Gas chromatography coupled to mass spectrometry (GC/MS) was used for analysis of the chemical profile of the EOs. Twenty compounds corresponding to 96% of the total essential oils were identified with 70% and 30% of the identified components being monoterpenes and sesquiterpenes, respectively. The essential oils ofT. minutarevealed promising antibacterial activities against the test pathogens withPseudomonas savastanoipv.phaseolicolabeing the most susceptible with mean inhibition zone diameters of 41.83 and 44.83 mm after 24 and 48 hours, respectively.The minimum inhibitory concentrations and minimum bactericidal concentrations of the EOs on the test bacteria were in the ranges of 24–48 mg/mL and 95–190 mg/mL, respectively. These findings provide a scientific basis for the use ofT. minutaessential oils as a botanical pesticide for management of phytopathogenic bacteria.

scholarly journals Antibacterial activity of Actinomycetes symbiont with seaweeds: a prosperous agent of animal antibacterial

2016 ◽  
Vol 1 (2) ◽  
Author(s):  
Bertoka Fajar S.P. Negara ◽  
. Riyanti ◽  
Bintang Marhaeni ◽  
Aradea B. Kusuma
Keyword(s):  
Antibacterial Activity ◽  
Secondary Metabolites ◽  
Pathogenic Bacteria ◽  
Pcr Amplification ◽  
Inhibition Zone ◽  
Antibacterial Activities ◽  
Diffusion Method ◽  
E Coli ◽  
Amplification Method ◽  
The Relationship

Circulation of synthetic antibacterial chemicals which is used continuously can cause resistance to bacteria. Alternative synthetic antibiotics research then comes up as an urgent need. Recently, the discovery of new antibacterial agents is looking for Actinomycetes. Actinomycetes are one of the active marine bacteria which produce secondary metabolites that could be used as antibacterial. The objective of this study was to evaluate the antibacterial activities of Actinomycetes symbiont with Halimeda macroloba, Gelidiella acerosa, and Turbinaria ornata in Pangandaran beach, Indonesia. A total of 41 Actinomycetes were isolated from 3 species of seaweed. Rep PCR amplification method was used to characterize and identify the relationship between samples. Primer BOX A1R (5’-CTACGGCAAGGCGACGCTGACGCTGACG-3’), 27F (5'-AGTTTGATCMTGGCTCAG-3') and 1492R (5'-TACGGYTACCTTGTTACGACTT-3') was used in amplification 16s DNA. Six isolates from the representative cluster dendogram were selected for secondary metabolites extraction using ethyl acetate solvent, meanwhile agar diffusion method was used as Antibacterial activity assay using E. coli, Proteus sp., and Enterobacter sp. as the bacterial test. The results showed that the activity of secondary metabolite extracts of Actinomycetes symbiont with Halimeda macroloba presented the highest inhibition zone on 3 bacterial tests. In the future, it could be a potential antibacterial agent against animal pathogenic bacteria.

scholarly journals Antibacterial Activity of Jatropha curcas (Linn) Leaves Extract a gainst Vibrio harveyi and Aeromonas hydrophila

2017 ◽  
Vol 19 (1) ◽  
pp. 11
Author(s):  
Nasrullah Bai Arifin ◽  
Imas Marthapratama ◽  
Ellana Sanoesi ◽  
Arief Prajitno
Keyword(s):  
Antibacterial Activity ◽  
Jatropha Curcas ◽  
Pathogenic Bacteria ◽  
Water Extract ◽  
Inhibition Zone ◽  
Disk Diffusion ◽  
Diffusion Method ◽  
Physic Nut ◽  
Disk Diffusion Method ◽  
Paper Disk

The use of natural products as an antimicrobial agent is an alternative way in the treating drug-resistant pathogens. A. hydrophila and V. harveyi are pathogenic bacteria both in freshwater and marine culture, respectively. Physic nut (Jatropha curcas, Linn) has been known as an herbal plant that has an antibacterial agent. The purpose of this study was to analyze inhibition ability of an extract of physic nut leaves on the growth of V. harveyi and A. hydrophila. In this study, water extract of physic nut leaves was determined to evaluate the antibacterial activity against V. harveyi and A. hydrophila using the Minimum Inhibitory Concentration Test (MIC) and Paper Disk Diffusion Method. The smallest concentration on the MIC was 0.10 g/ml for A. hydrophila and 0.15 g/ml for V. harveyi, respectively. The Paper Disk Diffusion Method used difference concentrations (0.10 g/ml; 0.15 g/ml; 0.20 g/ml; 0.25 g/ml; 0.30 g/ml; 0.35 g/ml; dan 0.40 g/ml) and Control (0 g/ml). The concentration of 0.35 g/ml had the highest inhibition zone diameter in 11.3±0.2 mm for A. hydrophila and 0.40 g/ml had the highest inhibition zone in 11.2±0.3 mm for V. harveyi. The result showed that water extract of physic nut leaves was able to inhibit both tested bacteria.

scholarly journals 18. Phytochemistry and Antibacterial Activity Test of Ethanol Extracts of Soursop flower (Annona muricata L.) against Salmonella enteritidis

2019 ◽  
Vol 13 (1) ◽  
pp. 132-137
Author(s):  
Zuraidawati Zuraidawati ◽  
Maryulia Dewi ◽  
Darmawi Darmawi ◽  
Sugito Sugito ◽  
Abdullah Hamzah ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Secondary Metabolites ◽  
Salmonella Enteritidis ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Annona Muricata ◽  
Activity Test ◽  
Phenolic And Flavonoid

           The purpose of this research was to identified the compounds in ethanol extract of soursop flower and determined the antibacterial activity of the soursop flower extract on Salmonella enteritidis. The phytochemistry screening was done to identify the secondary metabolite compounds of this extract. The concentrations used of ethanol extract of soursop flower were 10%, 20%, 30%, 40%, 50%, with ampicillin 10 μg/disc as the positive control and 10% Dimethyl Sulfoxide (DMSO) as the negative control. The antibacterial activity test of soursop flower ethanol extract against Salmonella enteritidis was held in vitro using the paper disc diffusion method by measuring the diameter of the inhibition zone. The phytochemistry screening showed that the ethanol extract of soursop flower had secondary metabolites such as alkaloids, phenolic, and flavonoid. The result of the antibacterial activity test showed that there was no inhibition zone (bright zone) at various concentrations. So, it can be concluded that soursop flower ethanol extract (Annona muricata L.) contained the secondary metabolites such as alkaloids, phenolic, and flavonoid, and also this extract had no antibacterial activity against Salmonella enteritidis

scholarly journals Antibacterial Activity of Rhamnus prinoides extracts against Staphylococcus aureus and Escherichia coli

2019 ◽  
Author(s):  
KINDU GETA
Keyword(s):  
Antibacterial Activity ◽  
Infectious Diseases ◽  
Pathogenic Bacteria ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Domestic Animal ◽  
Diffusion Method ◽  
Active Components ◽  
Microbial Infections ◽  
Ethanol Extracts

Abstract Background: Infectious diseases caused by bacteria have a large impact on public health. Increasing in resistant microbial infections intensified the search for new, safer, and more efficacious agents to combat serious microbial infections. Plants have played a central part in combating many diseases in human and domestic animal in many local communities, including Africa. Rhamnus prinoides is Ethiopian medicinal plants that have been used traditionally for the treatment of diverse infectious diseases. Therefore, this research was carried out to evaluate antibacterial activity Rhamnus prinoides extracts against pathogenic bacteria. Methods: The antimicrobial activity tests were carried out by the agar well diffusion method. Broth dilution and streak plate methods were used to determine MIC and MBC of extracts respectively. Results: The results revealed that ethanol extracts of fruits showed the highest antibacterial activity against standard strains of S.aureus with mean inhibition zone of 26±0.58, 28.33±1.2 and 33.33±0.9mm at 25, 50 and 100mg/ml respectively. The lowest mean MIC value (1.04±0.26 mg/ml) was recorded with ethanol extract of fruits against standard strains of S. aureus and the lowest mean MBC value (2.08±0.52 mg/ml) was recorded with ethanol extract of fruits against standard strains and clinical isolates of S. aureu. Conclusion: On the basis of the current findings, Rhamnus prinoides could be a good candidate in the search for new antibacterial agents from natural products against bacterial pathogens. Therefore, further studies are needed to study their toxicology and isolate the bio- active components from this plant.

scholarly journals Antibacterial Activity of Rhamnus prinoides extracts against Staphylococcus aureus and Escherichia coli

2019 ◽  
Author(s):  
kindu geta ◽  
Mulugeta Kibret
Keyword(s):  
Antibacterial Activity ◽  
Medicinal Plants ◽  
Pathogenic Bacteria ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Active Components ◽  
Microbial Infections ◽  
Ethanol Extracts ◽  
Broth Dilution

Abstract Medicinal plants offer a major and accessible source of health care to people living in developing countries. Increasing in resistant microbial infections intensified the search for new, safer, and more efficacious agents to combat serious microbial infections. Acanthus sennii is one of the medicinal plants used traditionally for treatment of different infectious diseases in Ethiopian. Therefore, this research was carried out to evaluate antibacterial activity of Acanthus sennii against pathogenic bacteria. The antimicrobial activity tests of the plant extracts against pathogenic bacteria were carried out by the agar well diffusion method. Broth dilution and streak plate method were used to determine MIC and MBC of extracts respectively. The results revealed that ethanol extracts of leaves showed the highest antibacterial activity against standard strains of S.aureus with inhibition zone of 14±0.58mm and 17±0.67mm at 50mg/ml and at 25mg/ml respectively. Ethanol extracts of buds showed the highest antibacterial activity against standard strains of S.aureus with inhibition zone of 25.67±0.67mm at 100mg/ml. The lowest mean MIC value (2.6±0.52 mg/ml) was recorded with ethanol extract of leaves against standard strains and clinical isolates of S. aureus. The lowest mean MBC value (4.2±1.04 mg/ml) was recorded with ethanol extract of leaves against standard strains of S. aureu. Acanthus sennii could be a good candidate in the search for new antibacterial agents from natural products against bacterial pathogens. Therefore, further studies are needed to study their toxicology and isolate the bio- active components from this plant. Key words: Acanthus sennii, Antibacterial activity, MIC, MBC, pathogenic bacteria

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