scholarly journals The histopathological changes at skin of German shepherd dogs associated with ringworm infection in directorate of K9 in Al-Diwanyia province

2014 ◽  
Vol 13 (1) ◽  
pp. 48
Author(s):  
H.Kh.Ul. Al-Karagoly
Keyword(s):  
Skin Lesion ◽  
Basal Layer ◽  
Microsporum Canis ◽  
Epidermal Layer ◽  
Histopathological Changes ◽  
German Shepherd ◽  
Histological Technique ◽  
Shoulder Region ◽  
Dermis Layer ◽  
Ringworm Infection

The current study was conducted to exam the histopathological changes of skin of German shepherd dogs suffering from ringworm infection. It may be the first study on this breed of dog in the Al-Diwanyia province. Seven German shepherd dogs from directorate of K9 in Al-Diwanyia province suffering from skin lesion were used in this study. Highly contaminated fur of infected dogs was cultured on Sabouraud’s dextrose agar (SDA). A five mm shaved biopsies of skin from all cases were taken from lesions present on shoulder region and after routine processes histological technique to evaluate the histopathological changes. The result were revealed that the colonies of Microsporum canis was flat, spreading, white to faint creamy-coloured with a dense buffy, granular to coarsely fluffy to hairy surface with radial grooves, and the histopathological exam of skin were showed presence of hyperkeratosis, spongiosis and marked dermal edema, skin bullae in the epidermal layer and accumulation of collagen fibers found of spores and hyphae of Microsporum in the stratum basale layer of epidermis and infiltration of eosinophils in the dermis layer directly beneath the basal layer of epidermis.

scholarly journals the Study of some histopathological changes occurring in white laboratory mice infected with Cutaneous Leishmaniasis in Al – diwaniyah province, Iraq.

2021 ◽  
Vol 26 (4) ◽  
Author(s):  
Lubna.A.Al-ibrahimi Edu ◽  
Hind Al-shabani ◽  
Marwa sami alwan
Keyword(s):  
Bile Duct ◽  
Mediterranean Region ◽  
Histological Study ◽  
Inflammatory Cells ◽  
Skin Lesions ◽  
Intracellular Parasite ◽  
Laboratory Mice ◽  
Histopathological Changes ◽  
Radial Arrangement ◽  
Dermis Layer

          Leishmaniasis is caused by an intracellular parasite . It is endemic in Asia, Africa, the Americas, and the Mediterranean region. Worldwide, 1.5 to 2 million new cases occur each year .The histological study of the liver tissue of white laboratory mice (Mus musculus) infected with L. major a parasite showed The presence of severe steatosis of hepatocytes Hepatocyte degeneration, And loss of the radial arrangement of hepatocytes, With heavy infiltration in inflammatory cells, especially phagocytes( Macrophage) with  Hyperplasia and congestion of the bile duct  .  As for histological sections of skin lesions taken from ear , Foot , tail ، were showed epidermal ulcerative , Accompanied by severe leaching of the dermis layer neutrophil ,polymorph lymphocytes ، with hemorrhage of ​​the dermis, with necrosis of the epidermal cells of all skin lesions in the ear, foot and tail.

Reconstructed interfollicular feline epidermis as a model for Microsporum canis dermatophytosis

2007 ◽  
Vol 56 (7) ◽  
pp. 971-975 ◽  
Author(s):  
Jeremy Tabart ◽  
Aline Baldo ◽  
Sandy Vermout ◽  
Betty Nusgens ◽  
Charles Lapiere ◽  
...  
Keyword(s):  
Histological Analysis ◽  
Pathogenic Fungus ◽  
Basal Layer ◽  
Histopathological Analysis ◽  
Microsporum Canis ◽  
Living Tissue ◽  
Cutaneous Infection ◽  
Interfollicular Epidermis

Microsporum canis is a pathogenic fungus that causes a superficial cutaneous infection called dermatophytosis. The complexity of mechanisms involved in dermatophytic infections makes relevant in vivo studies particularly difficult to perform. The aim of this study was to develop a new in vitro model of M. canis dermatophytosis using feline fetal keratinocytes in reconstructed interfollicular epidermis, and to investigate its relevance in studying the host–pathogen relationship. Histological analysis of reconstructed interfollicular feline epidermis (RFE) revealed a fully differentiated epidermis. A proliferation assay showed replicating cells only in the basal layer, indicating that RFE is a well-stratified living tissue, leading to the formation of a horny layer. Histopathological analysis of RFE infected by M. canis arthroconidia revealed that the fungus invades the stratum corneum and produces SUB3, a keratinase implicated in the infectious process. In view of these results, an M. canis dermatophytosis model on RFE seems to be a useful tool to investigate mechanisms involved in natural M. canis feline infections.

scholarly journals Seborrahic keratitis: a benign pigmented skin lesion management

2018 ◽  
Vol 6 (11) ◽  
pp. 3776
Author(s):  
Satish Chandra Kumar ◽  
Avinash Kumar Sinha ◽  
Saurabh Singh
Keyword(s):  
Skin Lesion ◽  
Histopathological Examination ◽  
Basal Layer ◽  
Excisional Biopsy ◽  
Pigmented Lesion ◽  
Lateral Canthus ◽  
Seborrhoeic Keratosis ◽  
Rhomboid Flap ◽  
Gross Examination ◽  
Rule Out

Seborrhoeic keratosis are formed from the basal layer of epidermal cells and contain melanocytes. Many terms such as senile wart, melanoacanthoma, basal cell papilloma, senile keratosis and seborrhoeic wart have been applied, but seborrhoeic keratosis is the most widely accepted term. In our case a 50-year-old female presented to the surgery OPD with a painless pigmented skin lesion 2.5cm lateral to the left lateral canthus and 1cm below the hairline of size of 4 *4cm. Pigmented lesion was from last 8 years and slowly progressive in nature and was not associated with any co morbid conditions. On clinical evaluation the clinical diagnosis of epidermal nevus was given and an excisional biopsy with rhomboid flap placement was performed and the tissue was sent for histopathological examination. Post operative period was uneventful and the histopathology report states the seborrhoeic keratosis. Hence, lesions for which the diagnosis is uncertain, based on the history and gross examination, should be biopsied for histopathologic examination to rule out malignancy.

A retrospective study of canine dilated cardiomyopathy (189 cases)

1997 ◽  
Vol 33 (6) ◽  
pp. 544-550 ◽  
Author(s):  
A Tidholm ◽  
L Jonsson
Keyword(s):  
Heart Failure ◽  
Atrial Fibrillation ◽  
Dilated Cardiomyopathy ◽  
Interstitial Fibrosis ◽  
Histopathological Changes ◽  
German Shepherd ◽  
Male Predominance ◽  
Doberman Pinschers ◽  
Mild Hyperglycemia ◽  
Canine Dilated Cardiomyopathy

The case records of 189 dogs (including 38 breeds) with congestive heart failure caused by dilated cardiomyopathy were studied retrospectively. Airedale terriers, boxers, Doberman pinschers, English cocker spaniels, Newfoundlands, St. Bernards, and standard poodles were over-represented. German shepherd dogs were under-represented. A male predominance was observed. Systolic murmurs were detected in 25% of the cases. Atrial fibrillation was the most common arrhythmia. Mild hyperglycemia and mild-to-moderate hypercholesterolemia were found in 38% and 33% of cases, respectively. Histopathological changes consisted of attenuated wavy fibers and interstitial fibrosis.

scholarly journals Epidemiological, Clinical and Laboratory study of Canine Dermatophytosis in Baghdad Governorate, Iraq

2019 ◽  
Vol 43 (1) ◽  
pp. 183-196
Author(s):  
Tareq Rifaaht Minnat
Keyword(s):  
Clinical Signs ◽  
Pathogenic Fungi ◽  
Common Species ◽  
Cold Climate ◽  
Cold Weather ◽  
Microsporum Canis ◽  
German Shepherd ◽  
Hair Coat ◽  
Significant Difference ◽  
Lower Infection

The aim of the present study was to isolate and identify of  the pathogenic fungi of canine dermatophytosis in Baghdad governorate Iraq, also study the epidemiology and describe the clinical signs of dermatophytosis, from January  2018 till December 2018. Out of 653 dogs 165 (25.3%) were with dermatomycoses and 103 (62.42%) dogs with dermatophytosis. The mycological diagnosis were conducted by direct microscopy and by culture the specimens on each sabouraud dextrose agar supplemented with chloramphenicol and cyclohxemide and dermatophytes test media.  The identified dermatophytes were represented Microsporum sp.(80.6%) appeared Microsporum canis (87.9%); M. audounii (8.4% );M. gypseum (3. 6 %) and Trichophyton spp (19.4%) in which divided between Trichophyton ruburum  (60.0 %) and T. terrestre (40.0%). Microsporum canis and Trichophyton ruburum were the most common species isolated (87.9%),(60.0 %) respectively. The overall prevalence of dermatomycosis and dermatophytosis from various parts region of Baghdad city were Al Karkh district (32.0%), (64.6%) and Al Rusafa district (22.4%), (60.4%) respectively. Moreover, a higher percentage of infection in young age 55 of 84 (65.47%) and lower infection in old age 8 of 18 (44.44%) with significant difference at P< 0.05. The effect of breeds on the prevalence of canine mycosis show high percentage of infection in German shepherd dog (47.96%) and lower infection in Bulldog breed (10.63%) as well as, high prevalence of dermatophytosis recorded in Rottweiler (100%), Pomeranian (83.3%) and lower prevalence (40.0 %) in each of Boxer and local dog breed. The relation of hair coat showed significantly high percentage of infection in long hair dogs (69.0%) than short hair coat (48.0%). There were no significant difference between the sex and habitat but, recorded a high (%) in shelters habitat (67.19%) and low (%) in plantation habitat (56.25%). The prevalence of infection was showed no signficantly high percentage of infection in cold climate winter (67.74%) in which low temperature and high humidity while low percentage of infection in dry climate autumn (44.4%). In addition the effect of season on the prevalence of dermatophytosis recognized by a higher (%) of infection in cold weather, winter (67.74%) and lower infection in dry weather, autumn (50.0%). Other dermatomycosis isolated were Chrysosporium Nannizziopsis dermatitidis (9.1%); Malassezia pacydermatis (7.9 %) and saprobe fungi from domestic dogs represented by  Alternaria alternate (21.4%); Aspergillus spp  (60.7%); Aspergillus flavus  (25.9%); Aspergillus fumigatus  (28.2%); Aspergillus niger  (23.5 %);  Aspergillus nidulans  (22.4 %); Penicillium sp. (4.3%); Curvularia sp  (3.6%) and  Rhizopus sp  (2.1%).

scholarly journals Comparative characteristics of different methods of treatment of microsporia

2021 ◽  
Vol 23 (104) ◽  
pp. 47-54
Author(s):  
Y. V. Martyniv ◽  
Ya. V. Kisera
Keyword(s):  
Antifungal Agent ◽  
Basal Layer ◽  
Antifungal Drugs ◽  
Common Disease ◽  
Microsporum Canis ◽  
T Suppressors ◽  
Source Of Infection ◽  
Therapeutic Measures ◽  
T Helpers ◽  
Methods Of Treatment

Microsporia is the most common disease of fungal etiology, most often caused by the pathogen Microsporum canis. Treatment of this disease requires a comprehensive approach, because the disease is dangerous to humans. Therefore, in the treatment of microspores, it is important not only to carry out therapeutic measures, but also to prevent the spread of the pathogen of the fungus in the environment and increase the immune status of the organism in the fight against infection. The main source of infection is cats. In order to determine the effectiveness of treatment of microsporia by various methods, studied of the blood and skin of guinea pigs infected with the pathogen M. canis. Sick animals were divided into three groups. The first group was treated with the systemic antifungal itraconazole and topical treatment with a solution of clotrimazole. Treatment of the second group was performed with a topical antifungal agent (1% solution of clotrimazole) with vaccination with the antifungal vaccine “Vakderm”. For the third group, the developed drugs were used – antifungal agent “Micromar” and immunostimulant “Biogluk”. During treatment, hematological and immunological blood researches and histological skin examinations were performed. During treatment with antifungal drugs (itraconazole and clotrimazole) the number of leukocytes decreases from 11.13 ± 0.72 to 7.13 ± 0.22, rod-shaped neutrophils from 15.76 ± 1.29 to 5.50 ± 0.76, and segmental increases from 12.17 ± 1.47 to 24.17 ± 2.27, decreases ESR from 5.67 ± 0.67 to 2.33 ± 0.42, which occurs when inhibiting the inflammatory response of the organism to infection. Thrombocytopenia (from 231.17 ± 7.60 to 184.33 ± 7.65) and eosinophilia (from 2.70 ± 0.73 to 7.33 ± 1.33) are also noted. There is a slight increase in T-helpers and a decrease in T-suppressors. Histologically, the infiltration of the dermis by histiocytes and  eosinophils under skin  persists. In the treatment of microsporia by treatment with 1 % solution of clotrimazole and vaccination with the vaccine “Vakderm” the results of studies showed that the number of leukocytes decreases from 11.13 ± 0.72 to 5.35 ± 0.31 (P < 0.01), rod-shaped neutrophils from 15.76 ± 1.29 to 7.67 ± 0.56, and segmental increases from 12.17 ± 1.47 to 22.17 ± 0.91 (P < 0.001), decreases). The number of T-helpers is increasing. The histological picture on day 7 is characterized by hyperkeratosis, and on day 14 the hyperemia of the basal layer of the epidermis persists. When using the antifungal drug “Micromar” and immunostimulant “Biogluk” the results of studies showed that the number of leukocytes decreases from 11.13 ± 0.72 to 6.95 ± 0.10, rod-shaped neutrophils from 15.76 ± 1.29 to 6.17 ± 0.65, and segmental increases from 12.17 ± 1.47 to 22.00 ± 0.86, decreases ESR from 5.67 ± 0.67 to 2.17 ± 0.31. Increases the number of T-helpers and the number of natural killers and T-suppressors is gradually decreasing. Histological changes are presented in the form of dilation of blood vessels and visualization of single erythrocytes in the dermis on day 14 of treatment.

scholarly journals Tinea Corporis Caused by Microsporum Canis in HIV Patient Treated For Neuromeningeal Cryptococcis: Report of A Nosocomial Outbreak

2018 ◽  
Vol 1 (04) ◽  
pp. 16-18
Author(s):  
Merad Yassine ◽  
Adjmi-Hamoudi Haiet ◽  
Tabet-Derraz Narjess ◽  
Merad Fatima Zohra Samia
Keyword(s):  
Disease Transmission ◽  
Cryptococcal Meningitis ◽  
Inpatient Treatment ◽  
Inpatient Care ◽  
Clinical Presentation ◽  
Tinea Corporis ◽  
Microsporum Canis ◽  
Newly Diagnosed ◽  
Nosocomial Outbreak ◽  
Ringworm Infection

We report a nosocomial outbreak of Microsporum canis during inpatient treatment for HIV newly diagnosed with cryptococcal meningitis. The clinical presentation, though very typical of ringworm infection, samples were collected and examined by direct microscopy and culture on Sabouraud’s dextrose agar, revealing Microsporum canis. It is strongly believed that outdoor cats living in the hospital spaces are the main cause of disease transmission. Measures to limit zoophilic pathogens are necessary in immunosuppressed inpatient care unit

The Micro-Anatomy of the Mammalian Epidermis

1953 ◽  
Vol s3-94 (28) ◽  
pp. 481-506
Author(s):  
P. B. MEDAWAR
Keyword(s):  
Physiological Activity ◽  
Continuous Process ◽  
Basal Layer ◽  
Genetic System ◽  
Skin Surface ◽  
The Body ◽  
Ground Substance ◽  
Epidermal Layer ◽  
Cell Divisions ◽  
Structure Density

The greater part of the superficial epidermis of mammals is of binary embryological origin, and consists of cells belonging to two distinct division lineages: that of the Malpighian or keratinizing system, and that of the melanocyte or pigmentary system. The melanocyte system can be artificially destroyed, or denied access to the epidermis in development, without impairment of any but the pigmentary activity of skin. The epidermis as a whole is a reproductively self-contained system, i.e. it is perpetuated by the division of cells that reside within the epidermis itself. It is argued that melanocytes as well as Malpighian cells are squamous in character, and that the functional melanocytes of the basal epidermal layer undergo a characteristic sequence of involutionary changes in the course of moving towards the skin surface to be flaked away. It follows that the entire epidermis, and not merely the Malpighian system, undergoes a continuous process of cellular renewal. Cell-divisions certainly occur in the basal epidermal layer; a number of difficulties of interpretation must be overcome before it can be held certain that divisions occur in more superficial layers as well. The Malpighian system has a cellular organization: there is no satisfactory evidence for the existence of intercellular cytoplasmic bridges that would endow it with a syncytial character. ‘Tonofibrils’ probably owe their origin to an artificial coarsening of a fine fibre-protein system within the cytoplasm of prickle cells. Elastic fibres probably play some part in anchoring the epidermis to its substratum; but there is evidence that the epidermis can be freed from the corium by disengaging the downwardly directed processes of Malpighian cells of the basal layer from the concentrated connective tissue ground-substance that forms the inner boundary of the dermo-epidermal interface. All the natural pigments of mammalian skins are melanins; melanocytes are the only seat of melanin formation, and pigmentary activity is the only function they are known to possess. Branches that arise from the perikarya of melanocytes are so arranged that each ends in close apposition to the superficial pole of a Malpighian cell and in some unknown manner causes pigment granules to enter into it. Tyrosine is probably the parental substrate in melanogenesis, and it is unlikely that more than one oxidase is responsible for the enzyme-mediated activities that lead to the formation of melanin. The fine structure and physiological activity of the Malpighian system varies from place to place on the body: many of the differences which appear to be due (and which, it is shown, could have been due) to differences of environment and manner of use are in fact of developmental origin and of ‘cellular genetic’ status. Such differences are conserved through repeated cell-divisions after the transplantation of particular areas of skin to anatomically unnatural environments. The regional anatomy of the melanocyte system is less complex. The melanocytes of the hair bulbs and of the superficial epidermis, responsible for the pigmentation of skin and hair respectively, are artificially interchangeable. It is therefore probable that they represent purely topographical variants of a homogeneous cellular genetic system. Differences of pigmentation between the variously coloured areas of a patched animal are due to differences between the pigmentary activities of individual melanocytes, and these are perpetuated in cellular heredity; they are not due to differences of structure, density, or distribution.

scholarly journals Case Report: Polyarteritis nodosa or complicated Henoch-Schonlein purpura (IgAV), a rare case

F1000Research ◽  
2018 ◽  
Vol 7 ◽  
pp. 49 ◽  
Author(s):  
Sajad Hasanzadeh ◽  
Seyedeh Maryam Alavi ◽  
Elahe Masnavi ◽  
Saeid Jokar ◽  
Maryam Rohani
Keyword(s):  
Skin Lesion ◽  
Polyarteritis Nodosa ◽  
Anal Verge ◽  
Basal Layer ◽  
Lower Extremities ◽  
Neurological Impairment ◽  
Skin Lesions ◽  
Filling Defect ◽  
Wall Thickening ◽  
The Right

Background: Polyarteritis nodosa (PAN) is a vasculitis that affects medium-sized arteries. PAN is a rare disease and requires a high clinical suspicion for diagnosis. PAN and HSP (newly named Immunoglobulin A-associated vasculitis) have narrowing differential diagnosis. Here, we reported a case of PAN. Case presentation: Our patient was a 65-year-old woman that came to hospital due to abdominal pain and skin lesion on the right upper and right lower extremities. All rheumatologic tests were negative. A biopsy of the skin lesion was reported as mild hyperkeratosis, slight spongiosis with intact basal layer. The dermis showed moderate to severe perivascular PMN infiltration with vessel wall degeneration and extravasation of RBCs. A colonoscopy reported diffuse mucosal erythema and erosions were seen in the rectum until 6cm of anal verge. An electromyogram test and nerve conduction velocity study of the upper extremities reported bilateral mild carpal tunnel syndrome, and in the right lower extremities mononeuritis multiplex could not be ruled out. Abdominopelvic CT scan reported diffuse wall thickening of terminal ileum associated with mesenteric fat and narrow enhancement of inferior Mesenteric artery with patchy filling defect. After evaluation, the patient received corticosteroid pulses plus cyclophosphamide. Conclusion: Diagnosis and treatment of PAN are important and PAN should be considered in a patient with skin lesions and neurological impairment.

scholarly journals Melanocytoma-acanthoma in a Dog

2019 ◽  
Vol 47 ◽  
Author(s):  
Renata Dalcol Mazaro ◽  
Douglas Miotto Lorensetti ◽  
Alana Pivoto Herbichi ◽  
Rafael Almeida Fighera
Keyword(s):  
Cellular Proliferation ◽  
Histopathological Examination ◽  
Basal Layer ◽  
The Other ◽  
Type I ◽  
Type Ii ◽  
Seborrheic Keratosis ◽  
German Shepherd ◽  
Fibrous Stroma ◽  
Well Differentiated

Background: Melanocytic neoplasms are skin tumors that often occur in dogs.  However, melanocytoma-acanthoma, also called melanoacanthoma, is a benign melanocytic neoplasm rarely reported in this species, which has been described only three times in the veterinary literature. Briefly, this tumor is characterized by a single, painless, darkly pigmented and firm cutaneous papule or nodule. Histologically, it is composed of mixed populations of well-differentiated melanocytes and keratinocytes, unlike traditional melanocytic tumors (melanoma and melanocytoma). These cells are arranged in lobules surrounded by collagenous stroma. Melanocytes are large epithelioid cells containing varying amounts of melanin. Keratinocytes form anastomosing trabeculae with peripheral palisading, and small cysts containing amorphous or laminated keratin. The definitive diagnosis of melanocytoma-acanthoma is based on histopathological findings. This report describes a case of melanocytoma-acanthoma in a dog in Brazil.Case: A 9-year-old female miniature Schnauzer dog was examined at the Veterinary Hospital of the Federal University of Santa Maria, where a single, firm, pigmented papule was found in the auricle. The lesion had started 15 days earlier. Hematological tests and serum biochemistry profile were normal. An excisional biopsy of the papule was surgically removed and subjected to histopathological examination. The tissue was fixed in 10% neutral buffered formalin, processed routinely and embedded in paraffin wax. Sections were stained with hematoxylin and eosin (HE). A histopathological examination revealed a nonencapsulated, well-defined, extensive, densely cellular proliferation located in dermis. This proliferation was composed of lobules and nests of well-differentiated stratified squamous epithelium closely associated with neoplastic melanocytes, surrounded by thin bundles of fibrous stroma. A diagnosis of melanocytoma-acanthoma was established based on these histological features.Discussion: The first description of melanocytoma-acanthoma in humans was as melano-epithelioma, classified into subtypes I and II. Both subtypes are benign neoplasms composed of well-differentiated melanocytes and keratinocytes, which are distinguished from one another based on the amount and distribution of melanocytes. Type I melano-epithelioma is characterized by proliferative lobules of melanocytes and keratinocytes, including melanocytes scattered diffusely among keratinocytes. Type II melano-epithelioma involves only the proliferation of keratinocytes, while melanocytes are limited to the basal layer of keratinocyte lobules. To clarify this condition, some authors use the term “melanoacanthoma” to indicate the above-described type I melano-epithelioma, and seborrheic keratosis to indicate type II melano-epithelioma. However, other authors use the term melanoacanthoma to denote the two conditions (types I and II melano-epithelioma). On the other hand, veterinary medicine does not recognize subtypes, instead using the term melanocytoma-acanthoma, and more recently, melanoacanthoma, to denote this cutaneous neoplasm. Melanocytoma-acanthoma in dogs was first reported in Spain, and involved a 2-year-old German shepherd dog. Later, two other cases were described in adult mixed-breed dogs, one in South Korea and the other in Libya. This is the first report of melanocytoma-acanthoma in a dog in Brazil. The gross and histopathological appearance of this case matches that described in the previous cases (a single, well-defined, pigmented cutaneous papule or nodule). Histologically, the differential diagnosis for melanocytoma-acanthoma includes melanoma, melanocytoma, trichoepithelioma, and sebaceous epithelioma. 

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