Features of seed growth and development of sproutings of the russian dereza (Lycium ruthenicum Murr.) under laboratory conditions and in culture in vitro

2021 ◽  
Vol 66 (2) ◽  
pp. 176-185
Author(s):  
E. A. Sedun ◽  
S. Sh. Abdirahimova ◽  
A. V. Zubarev ◽  
E. V. Spiridovich ◽  
V. N. Reshetnikov ◽  
...  
Keyword(s):  
Soil Salinity ◽  
Nutrient Medium ◽  
Callus Formation ◽  
Aral Sea ◽  
Biological Study ◽  
Positive Temperature ◽  
Culture In Vitro ◽  
Lycium Ruthenicum ◽  
Lycium Ruthenicum Murr

Russian dereza (Lycium ruthenicum Murr.) is a shrub plant that spreads on the territory of the dried Aral Sea in conditions of high soil salinity, dry and sharply continental climate. The fruits were collected and the features of germination of seeds of the russian dereza halophyte (Lycium ruthenicum Murr.) Under sterile and non-sterile conditions were studied. The optimal temperature and illumination regime for the germination of annual seeds of this species is a 16-hour photoperiod and a temperature of 25 °C, the germination of seeds in nonsterile conditions was: 4-year-old – 46 %; 2-year – 83; one-year – 96 %. It has been shown that L. ruthenicum plants remain viable for up to 4 years or more, on this basis, we attributed the seeds of this species to truly orthodox. When introduced into culture in vitro, it has been shown that multi-stage sterilization significantly reduces the viability of seeds and seedlings of Lycium ruthenicum Murr., which leads to a decrease in seed germination by up to 40 %. The optimal nutrient medium for the stable development of microshoots without anomalies, callus formation and initiation of root formation was MS with the addition of 1.0 mg/l 6-BAP to the nutrient medium, without sucrose. Maintaining the samples in the in vitro collection is carried out on a half-MS medium without hormones, without sucrose at a low positive temperature of 4 °C, illumination of ~500 lx and a photoperiod of 8 hours. The samples of L. ruthenicum in vitro can be used to develop methods of clonal micropropagation; for genotyping of samples and identification of molecular biomarkers of plant resistance to soil salinity; in the ex vitro morpho-biological study of plants resistant to salt stress.

scholarly journals Influence of the component composition of nutrient media upon the introduction into the culture in vitro of explants of Prikubanskoe and Kubanskoe Bagryanoe apple varieties

2020 ◽  
pp. 18-23
Author(s):  
E. V. Lobodina ◽  
I. I. Suprun ◽  
A. O. Avakimyan ◽  
E. A. Al-Nakib
Keyword(s):  
Fungal Infection ◽  
Nutrient Medium ◽  
High Efficiency ◽  
Cumulative Effect ◽  
Component Composition ◽  
Apple Trees ◽  
Nutrient Media ◽  
Culture In Vitro ◽  
Apple Varieties

The work reflects the aspects of introducing two varieties of apple trees into the culture in vitro: Prikubanskoe and Kubanskoe Bagryanoe. The aims of study included testing a new disinfection protocol that accurately regulates the content of active chlorine in the disinfecting solution, as well as studying the regenerative ability of explants of apple trees of the Prikubanskoe and Kubanskoe Bagryanoe varieties on different culture medium: Murashige and Skoog (MS) ¼ NH4 NO3 , and Kvorin-Lepuavra (QL). The disinfection protocol used made it possible to obtain an insignificant level of explant contamination with fungal infection in passage zero, which indicates the effectiveness of the tested extraction protocol. The contamination of explants in passage zero by fungal infection was insignificant, which indicates the high efficiency of the sterilizing agent. The level of bacterial infection is higher in the Kubanskoe Bagryanoe variety (21.3 % of the total amount of explants). The influence of the component composition of nutrient media on the primary growth of apple micro-shoots was revealed. The following results were obtained: the yield of viable explants of the Prikubanskoe variety grown on nutrient medium MS ¼ NH4 NO3 was 44,4 %, which is 16,4 % higher than explants grown on QL medium (28 %). Explants of the Kuba nskoe bagryanoe variety, in turn, have a yield of vital microplants grown on MS ¼ NH4 NO3 16.7 %, which is 30.4 % lower than explants grown on QL medium (47.1 %). The combined effect of the genotype and the composition of the nutrient medium on the development of explants was statistically significant for the studied parameters. The share of the cumulative effect of factors of the variety x medium is 74.93 %, the influence of the genotype is 20.72 %. The data obtained indicate the variety specificity of the nutrient medium during clonal micropropagation of these apple varieties.

scholarly journals Future outlook of plant growth regulators application influencing callus induction from different type explant Hyoscyamus muticus L. in vitro

2018 ◽  
Vol 7 (3) ◽  
pp. 10-13
Author(s):  
Walla Abdelmaksood Abdelazeez ◽  
Landysh Zavdetovna Khusnetdinova ◽  
Olga Arnoldovna Timofeeva
Keyword(s):  
Callus Induction ◽  
Nutrient Medium ◽  
Callus Formation ◽  
Stem Explants ◽  
Hyoscyamus Muticus ◽  
Skoog Medium ◽  
Murashige And Skoog Medium ◽  
Naphthylacetic Acid ◽  
Egyptian Henbane

The article shows the results concerning the problem of the influence of the hormonal composition of the medium on callus induction in isolated from different explants of Egyptian henbane areas (on the example of Hyoscyamus muticus L.). The authors study 11 variants of Murashige and Skoog medium supplemented with different concentrations and combination of auxins and cytokinins. It was important to find nutrient medium modification of Murashige and Skoog for callus induction. The article describes the fact that callus formation from different explant types of Hyoscyamus muticus L. in vitro was observed on Murashige and Skoog medium fortified with benzylaminopurine and naphthylacetic acid. It shows that the maximum callus induction was observed from root explants on Murashige and Skoog's medium supplemented with 0.5 mg/l of benzylaminopurine and 1.0 mg/l of naphthylacetic acid. And minimal callus formation was observed in the area with benzylaminopurine. Callus induction of leaf and stem explants both on the hormone-free nutrient medium and with the benzylaminopurine only was not observed. Thus, the results show that the frequency of callus formation with culturing root segment is higher compared to leaf and stem segment explants (on the example of Egyptian henbane in culture in vitro ). This work aims to inducing callus formation from various explants of Egyptian henbane, which can be used for plant regeneration or as a source for in vitro production of secondary metabolites.

scholarly journals The ability of maize Lancaster inbreds to callusogenesis in vitro under varying environmental conditions

2018 ◽  
Vol 22 ◽  
pp. 228-234 ◽  
Author(s):  
K. V. Derkach ◽  
V. V. Borysova ◽  
V. O. Maletskyi ◽  
T. M. Satarova
Keyword(s):  
Environmental Conditions ◽  
Callus Formation ◽  
Type I ◽  
Type Ii ◽  
Donor Plant ◽  
Culture In Vitro ◽  
Total Frequency ◽  
Plant Cultivation ◽  
The Impact

Aim. This work is focused on the estimation of the callusogenic potential of 10 maize Lancaster germplasm inbreds in comparison with well-known inbreds-standards with high callusogenic ability A188, Chi31 and PLS61, and the identification of genotypes with stable morphogenic callus formation under varying conditions of donor plant cultivation during three years of researches. Methods. Method of cell, tissue and organ culture in vitro. Field method. One-way and two-way analysis of variance. Results. For the investigated Lancaster inbreds the average multi-annual value of the total frequency of callusogenesis was 80.1 %, the frequency of morphogenic type I callus formation was 25.7 %, type II callus formation was 43.8 %, but for inbreds-standards these ones reached, respectively, 96.2 %, 12.2 % and 65.4 %. The level of callusogenesis varied depending on the year of investigations for all studied genotypes. The influence of genotype, year conditions and the combination of these factors on callus induction for most of the studied inbreds was significant. Conclusions. The impact of the interaction of a genotype and ecological factors of donor plant cultivation on morphogenic callusogenesis of type I as well as type II was the most significant forLancaster inbreds.Lancaster inbreds ДK298, ДK6080, ДK212 and ДK420-1 were the most stable under varying environmental conditions on the total frequency of callusogenesis, the frequencies of morphogenic and type II callus formation. None of the studied inbreds revealed the stability on type I callus formation frequency. Keywords: maize (Zea mays L.), Lancaster germplasm, callusogenesis, culture in vitro.

scholarly journals In vitro plant regeneration of Christmas cactus (Schlumbergera truncata (Haw.) Moran) by indirect morphogenesis

2021 ◽  
Vol 63 (1) ◽  
pp. 68-73
Author(s):  
Oksana Chornobrov ◽  
Svitlana Bilous
Keyword(s):  
Nutrient Medium ◽  
Culture Medium ◽  
Callus Formation ◽  
Shoot Proliferation ◽  
Root Induction ◽  
Optimal Conditions ◽  
In Vitro Plant Regeneration ◽  
Yellow Pigmentation ◽  
Dark Green

Abstract Plants of Schlumbergera truncata (Haw.) Moran were obtained by indirect morphogenesis from the segment section of shoots in vitro, they were multiplied and rooted. Also were determined the effect of the lighting regime, the composition of the nutrient medium on the consistency and frequency of callus formation. The studies were conducted during 2016–2018. The mode of effective sterilization (more than 90%) of S. truncata plant explants using 0.1% HgCl2 for 7–8 min was established. Optimal conditions for the induction of callus formation in stem node segments of S. truncata plants (rate more than 90% and significant growth) were created on MS (Murashige and Skoog 1962) nutrient medium supplemented with 1.0 mg/l BAP (6-benzylaminopurine) and 0.3 mg/l NAA (1-naphthylacetic acid) under conditions of placement on the nutrient medium and doing a significant number of cuts on the explants. The light intensity of 2.0–3.0 klx, obtained by a callus of dense consistency of dark green pigmentation, when using the thermostat condition without illumination, the callus had loose consistency, dark yellow pigmentation. It is established that the influence of the lighting regime and the composition of the nutrient medium on the frequency of callus formation is statistically significant. The largest number of shoots was obtained on the MS medium with the addition of 2.0 mg/l of BA. At the same times, shoot proliferation and root induction in such numbers were observed on MS culture medium with the addition of 0.5 mg/l BA and 0.5 mg/l kinetin (multiplication factor – 8.8±0.6 per 60-day cultivation cycle).

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