Researching of ultrasonic influence on callus formation, on maintenance of the general protein, on enzymes activity and inhibitor of tripsin in extracts from the soybean of the grade ≪Altom≫ and plant cells growth of culture in vitro

2008 ◽  
Author(s):  
Marina E. Lamberova ◽  
Alena S. Kosolapova
Keyword(s):  
Callus Formation ◽  
Plant Cells ◽  
Culture In Vitro ◽  
Enzymes Activity ◽  
General Protein ◽  
Ultrasonic Influence

Features of seed growth and development of sproutings of the russian dereza (Lycium ruthenicum Murr.) under laboratory conditions and in culture in vitro

2021 ◽  
Vol 66 (2) ◽  
pp. 176-185
Author(s):  
E. A. Sedun ◽  
S. Sh. Abdirahimova ◽  
A. V. Zubarev ◽  
E. V. Spiridovich ◽  
V. N. Reshetnikov ◽  
...  
Keyword(s):  
Soil Salinity ◽  
Nutrient Medium ◽  
Callus Formation ◽  
Aral Sea ◽  
Biological Study ◽  
Positive Temperature ◽  
Culture In Vitro ◽  
Lycium Ruthenicum ◽  
Lycium Ruthenicum Murr

Russian dereza (Lycium ruthenicum Murr.) is a shrub plant that spreads on the territory of the dried Aral Sea in conditions of high soil salinity, dry and sharply continental climate. The fruits were collected and the features of germination of seeds of the russian dereza halophyte (Lycium ruthenicum Murr.) Under sterile and non-sterile conditions were studied. The optimal temperature and illumination regime for the germination of annual seeds of this species is a 16-hour photoperiod and a temperature of 25 °C, the germination of seeds in nonsterile conditions was: 4-year-old – 46 %; 2-year – 83; one-year – 96 %. It has been shown that L. ruthenicum plants remain viable for up to 4 years or more, on this basis, we attributed the seeds of this species to truly orthodox. When introduced into culture in vitro, it has been shown that multi-stage sterilization significantly reduces the viability of seeds and seedlings of Lycium ruthenicum Murr., which leads to a decrease in seed germination by up to 40 %. The optimal nutrient medium for the stable development of microshoots without anomalies, callus formation and initiation of root formation was MS with the addition of 1.0 mg/l 6-BAP to the nutrient medium, without sucrose. Maintaining the samples in the in vitro collection is carried out on a half-MS medium without hormones, without sucrose at a low positive temperature of 4 °C, illumination of ~500 lx and a photoperiod of 8 hours. The samples of L. ruthenicum in vitro can be used to develop methods of clonal micropropagation; for genotyping of samples and identification of molecular biomarkers of plant resistance to soil salinity; in the ex vitro morpho-biological study of plants resistant to salt stress.

scholarly journals The ability of maize Lancaster inbreds to callusogenesis in vitro under varying environmental conditions

2018 ◽  
Vol 22 ◽  
pp. 228-234 ◽  
Author(s):  
K. V. Derkach ◽  
V. V. Borysova ◽  
V. O. Maletskyi ◽  
T. M. Satarova
Keyword(s):  
Environmental Conditions ◽  
Callus Formation ◽  
Type I ◽  
Type Ii ◽  
Donor Plant ◽  
Culture In Vitro ◽  
Total Frequency ◽  
Plant Cultivation ◽  
The Impact

Aim. This work is focused on the estimation of the callusogenic potential of 10 maize Lancaster germplasm inbreds in comparison with well-known inbreds-standards with high callusogenic ability A188, Chi31 and PLS61, and the identification of genotypes with stable morphogenic callus formation under varying conditions of donor plant cultivation during three years of researches. Methods. Method of cell, tissue and organ culture in vitro. Field method. One-way and two-way analysis of variance. Results. For the investigated Lancaster inbreds the average multi-annual value of the total frequency of callusogenesis was 80.1 %, the frequency of morphogenic type I callus formation was 25.7 %, type II callus formation was 43.8 %, but for inbreds-standards these ones reached, respectively, 96.2 %, 12.2 % and 65.4 %. The level of callusogenesis varied depending on the year of investigations for all studied genotypes. The influence of genotype, year conditions and the combination of these factors on callus induction for most of the studied inbreds was significant. Conclusions. The impact of the interaction of a genotype and ecological factors of donor plant cultivation on morphogenic callusogenesis of type I as well as type II was the most significant forLancaster inbreds.Lancaster inbreds ДK298, ДK6080, ДK212 and ДK420-1 were the most stable under varying environmental conditions on the total frequency of callusogenesis, the frequencies of morphogenic and type II callus formation. None of the studied inbreds revealed the stability on type I callus formation frequency. Keywords: maize (Zea mays L.), Lancaster germplasm, callusogenesis, culture in vitro.

FACTORS AFFECTING THE REGENERATION OF PEPPER (CAPSICUM ANNUUM L.)

HortScience ◽  
1990 ◽  
Vol 25 (9) ◽  
pp. 1120G-1120
Author(s):  
J. L. Jacobs ◽  
C. T. Stephens
Keyword(s):  
Growth Hormone ◽  
Silver Nitrate ◽  
Callus Formation ◽  
Leaf Explants ◽  
Regeneration System ◽  
Factors Affecting ◽  
Nitrate Concentrations ◽  
Leaf Differentiation ◽  
Whole Plants

Several growth hormone combinations and silver nitrate concentrations were examined for their effect on regeneration of different pepper genotypes. Primary leaf explants from in vitro seedlings were cultured on a revised Murashige and Skoog medium supplemented with auxin, cytokinin and 1.6% glucose. Combinations of different concentrations of indole-3-acetic acid (IAA), 0-5 mg/l, and 6-benzylaminopurine (BAP), 0-5 mg/l, were tested to determine the most effective medium for shoot primordium formation. Experiments with IAA and BAP did not result in a specific growth hormone combination appropriate for regeneration of all genotypes tested. Of the silver nitrate concentrations tested, 10 mg/l resulted in the best shoot and leaf differentiation and reduced callus formation. Differences in organogenic response of individual genotypes were evaluated on a single regeneration medium. Whole plants were regenerated from 11 of 63 genotypes examined. Based on these experiments, a reproducible regeneration system for pepper was developed with a total of 500 plants regenerated to date.

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