scholarly journals Literature review: Economic value of utilization of crab shell waste (Case study of PT. Toba Surimi Industri in Tanjungpinang City, Riau Island Province)

2021 ◽  
Vol 5 (2) ◽  
pp. 49-52 ◽  
Author(s):  
Mardiana E Fachry ◽  
Alpiani Alpiani
Keyword(s):  
Literature Review ◽  
Economic Value ◽  
Scientific Paper ◽  
Crab Shell ◽  
Shell Weight ◽  
Shell Waste ◽  
Crab Shell Waste ◽  
Additional Value

Writing this scientific paper aims to determine the economic value of the potential waste of crab shells produced by PT. Toba Surimi Industry in Tanjungpinang City, Riau Islands Province. The company has not utilized crab shell waste. In contrast, crab shell waste can provide economic value that provides a lot of additional value. Crab shell waste can be processed into chitosan, which has many functions in various sectors, both in the fisheries, automotive and health industries, and other sectors. Chitosan is produced from the chitin substance contained in crab shells through deproteination, demineralization, and deacetylation. The economic value that can generate from the utilization of crab shell waste PT. Toba Surimi Industry when processed into chitosan with a crab shell weight of 270 kg/day, which is Rp. 40.500.000/day.

scholarly journals Evaluation of enzymatic activity and identification of potent proteolytic and chitinolytic bacteria isolated from crab shell waste

2019 ◽  
Vol 21 (1) ◽  
Author(s):  
MOH DLIYAUDDIN ◽  
TRI ARDYATI ◽  
SUHARJONO
Keyword(s):  
Enzymatic Activity ◽  
16S Rdna ◽  
Skim Milk ◽  
Chitinase Activity ◽  
Crab Shell ◽  
Chitinolytic Bacteria ◽  
Rdna Sequences ◽  
16S Rdna Sequences ◽  
Shell Waste ◽  
Crab Shell Waste

Abstract. Dliyauddin M, Ardyati T, Suharjono. 2020. Evaluation of enzymatic activity and Identification of Potent Proteolytic and Chitinolytic Bacteria Isolated from Crab Shell Waste. Biodiversitas 21: 211-218. Proteolytic and chitinolytic bacteria are needed to degrade protein-chitin fibers in crab shell waste. These bacteria are important for enhancing the degradation rate of crab shell waste in the environment. This study aims to study the enzymatic activity and identify potent proteolytic and chitinolytic bacteria based on 16S rDNA sequences. The bacteria were isolated from crab shell waste using skim milk agar and colloidal chitin agar for proteolytic and chitinolytic bacteria, respectively. The bacterial isolates were screened based on their protease and chitinase activity using casein and chitin as substrates, respectively. The selected isolates were identified based on 16S rDNA sequences similarity. A total of 20 proteolytic and 22 chitinolytic bacteria were successfully isolated from the samples. The isolate of AP9, AP5, and BP14 had a high protease activity. However, only BP14 and CK20 showed a high chitinase activity. The isolate of AP9 was identified as Bacillus subtilis, AP5, and CK20 were identified as Bacillus licheniformis, and BP14 was identified as Bacillus cereus. AP5 dan CK20 were classified as non-pathogenic isolates.

scholarly journals Evaluation of Proteolytic and Chitinolytic Activities of Indigenous Bacillus Speciesfrom Crab Shell Waste

2020 ◽  
Vol 10 (1) ◽  
pp. 1-5
Author(s):  
Moh Dliyauddin ◽  
◽  
Tri Ardyati ◽  
Suharjono Suharjono
Keyword(s):  
Bacillus Licheniformis ◽  
Incubation Time ◽  
Enzymatic Reaction ◽  
Enzymatic Activities ◽  
Bacillus Species ◽  
Logarithmic Phase ◽  
Crab Shell ◽  
Degradation Rates ◽  
Shell Waste ◽  
Crab Shell Waste

The increase of crab meat export activities in Indonesia leads to the accumulation of crab shell waste in a massive amount that can naturally degrade and causing bad odor. Naturally, microorganisms will degrade this waste through fermentation and enzymatic reaction, including protease and chitinase due to its high content of chitin and protein. Bacillus is the most potential bacteria to degrade crab shell waste, and indigenous Bacillus from this waste can increase the degradation rates. The aims of this study were to evaluate the proteolytic and chitinolytic activities of indigenous Bacillus species from crab shell waste. Bacillus cereus BP14 and Bacillus licheniformis CK20 as the chitinolytic bacteria, and Bacillus subtilis AP9 and Bacillus licheniformis AP5 as the proteolytic bacteria were isolated from crab shell waste and identified based on the 16S rDNA sequences. The bacterial isolates were grown in skim milk broth for proteolytic characterization and colloidal chitin broth for chitinolytic characterization. The growth rates of each bacteria were determined through the growth curves. The enzymatic activities were determined based on the international standard for protease and chitinase enzyme activities together with growth curve sampling to determine the best incubation time for obtaining the highest enzymatic activities. From the shortest to the longest generation times of the Bacillus species obtained were B. subtilis AP9, B. licheniformis CK20, B. cereus BP14, and B. licheniformis AP5, respectively. The best incubation time for producing the highest enzymatic activity varied among species. However, the end of the logarithmic phase was similar. All of the Bacillus species obtained from this study exhibited chitinolytic and proteolytic activity. Therefore, it can be used as promising candidates of biodegradation agents inenhancing the degradation rates of crab shell wastes.

scholarly journals Optimization Of Chitosan Preparation From Crab Shell Waste

REAKTOR ◽  
2017 ◽  
Vol 7 (1) ◽  
pp. 37 ◽  
Author(s):  
M. Djaeni
Keyword(s):  
Maximum Yield ◽  
Operation Time ◽  
Process Variable ◽  
Raw Material ◽  
Crab Shell ◽  
Step Size ◽  
Time Step ◽  
Operation Temperature ◽  
Shell Waste ◽  
Crab Shell Waste

Crab shell waste from seafood restaurant is potential to be used as chitosan source. This material containing 20-30% of chitin which could be converted into chitosan through deacetylation process. While, chitin could be isolated from crab shell by deproteination and demineralization. Chitosan is fine chemical used to adsorb fat from body, heavy metal adsorbent, and medicine. This research looked into the prospect of crab shell as raw material to produce chitosan. In this case, the process variable of chitosan preparation was investigated involving operation time and  NaOH concentration to determine optimum condition. Whereas, the other parameters including operation temperature, NaOH to chitin ratio is respectively fixed at 70-80 0C and 5:1. As respons, the yield of chitosan is calculated. In this case, the deacetylation time is varied from 1-4 hours with the time step 1 hours and the concentration of NaOH  is change from 20-50% with the step size of 10%. The results showed that the maximum yield of chitosan is 9,15%, which could be achieved at operation time of 3 hours and NaOH concentration of 20%.Keywords : crab shell, deproteination, demineralization, deacetylation, chitosan

CHITOSAN INHIBITION FROM SHELL OF WASTE CRAB CULTIVATION “SOKA” (SCYLLA SP) AGAINST 4 HISTAMINE FORMING BACTERIA ISOLATE IN EUTHYNNUS AFFINIS

2017 ◽  
Vol 7 (1) ◽  
pp. 20
Author(s):  
Siti Aisyah ◽  
Agustiana Agustiana ◽  
Rabiatul Adawyah ◽  
Candra Candra
Keyword(s):  
Serratia Marcescens ◽  
Histidine Decarboxylase ◽  
Bacterial Symbionts ◽  
Crab Shell ◽  
Shell Waste ◽  
Three Stages ◽  
Euthynnus Affinis ◽  
Crab Shell Waste ◽  
Citrobacter Amalonaticus

The purpose of this study was to determine the inhibition of chitosan from crab cultivation waste "soka" (Scylla sp) against histamine-forming bacteria in tuna (Euthynnus affinis). The formation of histamine on the cob caused by bacteria that can produce the enzyme histidine decarboxylase to transform histidine into histamine. Several studies have described the ability of chitosan to inhibit the activity of histamine-forming bacteria. In this research, three stages, the first characterization of chitosan from crab shell waste cultivation "soka". Second, isolation of bacteria producing histamine on the cob and a third is testing the activity of chitosan against bacteria producing histamine. Characteristics of chitosan from crab shell waste cultivation "soka" is the water content of 10.07 %, 0.20 % fat, 1.42 % ash content, protein content 3.18 % and 58.99 % degree of deacetylation. The result of the isolation of bacterial symbionts on the cob re-examined in the formation of histamine thus obtained 4 isolates that Serratia marcescens, Enterobacteriaceae sp, Enterobacter gergoviae and Citrobacter amalonaticus. Testing inhibition of chitosan against bacteria Serratia marcescens, Enterobacteriaceae sp, Enterobacter gergoviae and Citrobacter amalonaticus obtained a clear zone, respectively, are 0.54 ± 0.03 cm; 0.59 ± 0.01 cm; 0.41 ± 0.01 cm; and 0.40 ± 0.05 cm.

scholarly journals Drivers of Firm Performance: Exploring Quantitative and Qualitative Approaches

2017 ◽  
Vol 12 (1) ◽  
pp. 79-84 ◽  
Author(s):  
Mihaela Herciu
Keyword(s):  
Firm Performance ◽  
Literature Review ◽  
Balanced Scorecard ◽  
Economic Value ◽  
Qualitative Approach ◽  
The Other ◽  
Bottom Line ◽  
Qualitative Approaches ◽  
Quantitative Indicators

AbstractThe main purpose of this paper is to identify the drivers of firm performance by exploring both quantitative indicators - based on accounting profitability, shareholder value and economic value – and qualitative approach – based on balanced scorecard and triple bottom line. A literature review will be provided in order to obtain an optimum mix of quantitative and qualitative drivers for firm performance, on one hand, and a case study will be conducted for emphasizing the importance of both approaches, on the other hand.

scholarly journals Preparation and Characterization of Chitosan from Indonesian Tambak Lorok Shrimp Shell Waste and Crab Shell Waste

2018 ◽  
Vol 17 (9) ◽  
pp. 446-453 ◽  
Author(s):  
Dina Sugiyanti ◽  
Purnama Darmadji ◽  
Sri Anggrahini ◽  
Chairil Anwar ◽  
Umar Santoso
Keyword(s):  
Crab Shell ◽  
Shrimp Shell ◽  
Shell Waste ◽  
Shrimp Shell Waste ◽  
Crab Shell Waste

scholarly journals Temperature Effect of Water Coagulation Bath on Chitin Fiber Prepared through Wet-Spinning Process

Polymers ◽  
2021 ◽  
Vol 13 (12) ◽  
pp. 1909
Author(s):  
Khoa Dang Nguyen
Keyword(s):  
Phase Inversion ◽  
Room Temperature ◽  
Biomedical Application ◽  
Coagulation Bath ◽  
Wet Spinning ◽  
Crab Shell ◽  
Shell Waste ◽  
Different Temperatures ◽  
Spinning Process ◽  
Crab Shell Waste

Chitin was chemically extracted from crab shell waste and dissolved in N,N-dimethyl acetamine/5% lithium chloride (DMAc/5% LiCl) at room temperature to obtain 1% and 2% concentrations of chitin solution. Chitin fibers were prepared by phase inversion at different temperatures of water coagulation bath at 5, 20, and 60 °C. The deconvolution of FTIR spectra indicated that the area portion of the intermolecular hydrogen bonding NH…OC increased at 60 °C due to the higher density of the chitin segment in the fiber. As a result, scanning electron microscope (SEM) measurement suggests that a denser structure of the chitin fiber was observed when the temperature of the coagulation bath increased. In addition, the resultant chitin fibers generated better mechanical properties relative to the amount of chitin concentration and temperature. At 2% of chitin solution, the tensile strength significantly increased from 80 to 182 MPa for the fiber obtained at temperatures of 5 and 60 °C of the water coagulation bath, respectively. Meanwhile, the water content in the fiber significantly decreased from 1101% to 335%. This green synthesis route has high potential for the fabrication of the fiber as future material of interest for biomedical application.

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