scholarly journals Keragaman Stomata Okra (Abelmoschus esculentus L. (Moench)

2018 ◽  
Vol 2 (2) ◽  
pp. 41
Author(s):  
Sulassih , ◽  
Joko Mulyono ◽  
Muhamad Syukur ◽  
Sofyan Zaman ◽  
Muharama Yora ◽  
...  
Keyword(s):  
Water Management ◽  
Stomatal Density ◽  
Cell Number ◽  
Abelmoschus Esculentus ◽  
High Yield ◽  
Stomatal Index ◽  
Plant Breeding Program ◽  
Density Index ◽  
Stomatal Length ◽  
Stomatal Number

Okra (Abelmoschus esculentus L. (Moench.) is high nutrition compound potential for diabetes and Alzheimer. Yield is one of the characters have to improve in plant breeding program through stomata characterization. The variability for 14 genotypes showed significantly for stomatal length, stomatal width, epidermal cell number, chloroplast number, stomatal number, stomatal index and stomatal density. Stomatal density for P2 was  256.80 mm2 that was nonsignificant with Zahira (265.31 mm2) and Naila (204.08 mm2). Genotypes P2, Zahira and Naila showed low stomatal density that mean efficiency for water management in leaves. The high stomatal index causing high photosynthesis and high yield. Stomatal index were high for genotypes P1 (40.31) and P2 (35.69). Naila has low stomatal index but Zahira as significant as genotype P2. Zahira could be high yield genotype candidate.Keyword : candidate, density, index, genotype

Dicentrine and Dicentrinone Isolated from Stephania tetrandrae Radix Suppress HepG2 Proliferation through Inhibiting PDI Activity

2021 ◽  
Vol 15 (5) ◽  
pp. 396-407
Author(s):  
Mojiao Zhao ◽  
Chao Zhang ◽  
Dong Zhang ◽  
Siyu Zhu ◽  
Tianjiao Liu ◽  
...  
Keyword(s):  
Protein Disulfide Isomerase ◽  
Cell Number ◽  
High Yield ◽  
Physical Interaction ◽  
Dependent Manner ◽  
Human Hepatoma ◽  
Human Hepatoma Cells ◽  
Disulfide Isomerase ◽  
Anti Cancer ◽  
Protein Disulfide

Inhibition of protein disulfide isomerase (PDI) has been attempted as a promising anti-cancer strategy. However, there is still no currently available PDI inhibitors approved for clinical use. Here, we isolated seven high yield alkaloids from Stephaniae tetrandrae Radix (STR), a medical herb frequently prescribed in anti-tumor condition, and identified two potent natural PDI inhibitors, dicentrine and dicentrinone. Among the seven alkaloids isolated, dicentrinone (1), dicentrine (2), tetrandrine (4), and fangchinoline (5) could significantly reduce cell viability in a dosage dependent manner detected by MTT assay in human hepatoma cells. To examine whether the candidate compounds are potent PDI inhibitors, we performed insulin turbidity assay and found dicentrine and dicentrinone, but not tetrandrine and fangchinoline, could effectively inhibit PDI activity, with IC50 of 56.70 μM and 43.95 μM respectively. Meanwhile, dicentrine and dicentrinone failed to further reduce the cell number index when co-treated with siRNA of PDI, suggesting the compounds behave as PDI inhibitors. Furthermore, dicentrinone and dicentrine have been successfully docked to the active pocket of PDI (PDB #3UEM) by molecular docking, suggesting the existence of physical interaction between compounds and PDI. Our results suggested that dicentrine and dicentrinone may be developed into safe PDI inhibitors.

scholarly journals Difference between emergent aquatic and terrestrial monocotyledonous herbs in relation to the coordination of leaf stomata with vein traits

AoB Plants ◽  
2020 ◽  
Vol 12 (5) ◽  
Author(s):  
Wanli Zhao ◽  
Peili Fu ◽  
Guolan Liu ◽  
Ping Zhao
Keyword(s):  
Water Supply ◽  
Aquatic Plants ◽  
Stomatal Density ◽  
Supply And Demand ◽  
Terrestrial Plants ◽  
Major Vein ◽  
Significant Difference ◽  
Leaf Veins ◽  
Vein Diameter ◽  
Stomatal Length

Abstract Emergent aquatic plants mostly occur in shallow waters and root in bottom substrates, but their leaves emerge from the water surface and are thus exposed to air, similar to the leaves of terrestrial plants. Previous studies have found coordination between leaf water supply and demand in terrestrial plants; however, whether such a coordination exists in emergent aquatic plants remains unknown. In this study, we analysed leaf veins and stomatal characteristics of 14 emergent aquatic and 13 terrestrial monocotyledonous herb species (EMH and TMH), with 5 EMH and 8 TMH belonging to Poaceae. We found that EMH had significantly higher mean leaf area, leaf thickness, stomatal density, stomatal number per vein length and major vein diameter, but lower mean major vein length per area (VLA) and total VLA than TMH. There was no significant difference in stomatal length, minor VLA and minor vein diameter between the two groups. Stomatal density and total VLA were positively correlated among the EMH, TMH, as well as the 8 Poaceae TMH species, but this correlation became non-significant when data from both the groups were pooled. Our results showed that the differences in water supply between emergent aquatic and terrestrial plants modify the coordination of their leaf veins and stomatal traits.

scholarly journals Growth and leaf epidermal response of three Sesamum indicum varieties to industrial effluent irrigation

2017 ◽  
Vol 52 (1) ◽  
pp. 1-6 ◽  
Author(s):  
AA Abdul Rahaman ◽  
OM Olaniran ◽  
FA Oladele
Keyword(s):  
Seedling Growth ◽  
Stomatal Density ◽  
Industrial Effluent ◽  
Industrial Effluents ◽  
Sesamum Indicum ◽  
Proper Treatment ◽  
Trichome Density ◽  
Stomatal Index ◽  
Stomatal Size ◽  
Epidermal Features

The effect of industrial effluents was studied with respect to growth and leaf anatomy of three Sesamum indicum varieties (NGB 00931, NGB 00937 and NGB 00939). Industrial effluents (25%, 50%, 75% and 100%) from two industries are used to irrigate the plants. Although, the control plants possessed larger leaves and longer stems than the effluent-treated plants, at lower concentration, the plant growth is relatively higher. Gradual decrease in the germination of seeds and seedling growth with increase in effluent concentration was observed. The best germination and seedling growth was observed at the 25% concentration. Leaf epidermal features (stomatal density, stomatal index, stomatal size, trichome density, tricome index, trichome size and number of epidermal cells) are more influenced in the effluent-treated plants than in the control plants from the Peace Standard Pharmaceutical Industry than in the effluent from the Global Soap & Detergent Industry. Thus the industrial effluents can be safely used for irrigation purposes with proper treatment and dilution at 25%.Bangladesh J. Sci. Ind. Res. 52(1), 1-6, 2017

scholarly journals Rare allele of a previously unidentified histone H4 acetyltransferase enhances grain weight, yield, and plant biomass in rice

2014 ◽  
Vol 112 (1) ◽  
pp. 76-81 ◽  
Author(s):  
Xian Jun Song ◽  
Takeshi Kuroha ◽  
Madoka Ayano ◽  
Tomoyuki Furuta ◽  
Keisuke Nagai ◽  
...  
Keyword(s):  
Plant Biomass ◽  
Rare Allele ◽  
Cell Number ◽  
Yield Component ◽  
Grain Weight ◽  
High Yield ◽  
Regulation Of Transcription ◽  
Histone H4 ◽  
Component Trait ◽  
Weight Yield

Grain weight is an important crop yield component; however, its underlying regulatory mechanisms are largely unknown. Here, we identify a grain-weight quantitative trait locus (QTL) encoding a new-type GNAT-like protein that harbors intrinsic histone acetyltransferase activity (OsglHAT1). Our genetic and molecular evidences pinpointed the QTL-OsglHAT1’s allelic variations to a 1.2-kb region upstream of the gene body, which is consistent with its function as a positive regulator of the traits. Elevated OsglHAT1 expression enhances grain weight and yield by enlarging spikelet hulls via increasing cell number and accelerating grain filling, and increases global acetylation levels of histone H4. OsglHAT1 localizes to the nucleus, where it likely functions through the regulation of transcription. Despite its positive agronomical effects on grain weight, yield, and plant biomass, the rare allele elevating OsglHAT1 expression has so far escaped human selection. Our findings reveal the first example, to our knowledge, of a QTL for a yield component trait being due to a chromatin modifier that has the potential to improve crop high-yield breeding.

scholarly journals Stomatal Density In Miscanthus Leaves

2015 ◽  
Vol 18 (2) ◽  
pp. 45-48
Author(s):  
Žaneta Pauková ◽  
Zuzana Jureková
Keyword(s):  
Leaf Area ◽  
Unit Area ◽  
Stomatal Density ◽  
Arable Land ◽  
The South ◽  
Stomatal Size ◽  
Stomatal Length ◽  
Non Destructive

Abstract In this study, the number of stomata per unit area was determined in the perennial energy grass of two genotypes Miscanthus × giganteus (Greef et Deuter) and M. sinensis ‘Tatai’ on arable land in the South-western Slovakia. The dynamics of stomatal density per mm2 was determined by non-destructive method in three randomly selected clumps. The number of stomata per mm2 of area varied from 125 ±23 to 327 ±47, stomatal length ranged from 33.9 ±4.7 μm to 35.3 ±3.4 μm, and stomatal width ranged from 17.5 ±2.3 μm to 18.8 ±2.8 μm in the two genotypes. We confirmed ontogenetic heterogeneity of leaf area size and stomatal size parameters.

Weak co-ordination between vein and stomatal densities in 105 angiosperm tree species along altitudinal gradients in Southwest China

2016 ◽  
Vol 43 (12) ◽  
pp. 1126 ◽  
Author(s):  
Wan-Li Zhao ◽  
Ya-Jun Chen ◽  
Timothy J. Brodribb ◽  
Kun-Fang Cao
Keyword(s):  
Water Balance ◽  
Tree Species ◽  
Altitudinal Gradient ◽  
Stomatal Density ◽  
Montane Forests ◽  
Vein Density ◽  
Tropical Montane Forests ◽  
Leaf Level ◽  
Angiosperm Species ◽  
Stomatal Length

Leaf-level water balance, as revealed by a correlation between stomatal density (SD) and vein density (VD), has been reported in some plants. However, the generality of this correlation and how it may be affected by altitude changes are unclear. Here, we investigated whether this balance is maintained across tree species of diverse families along a large altitudinal gradient. We measured leaf area (LA), SD, stomata length (SL), and VD in 105 angiosperm species across two altitudinal ranges, 800–1400 m above sea level (a.s.l.) in tropical montane forests (TMF) and 2000–2600 m a.s.l. in subtropical montane forests (SMF) in Yunnan, South-west China. The average SD was independent of altitude in both regions. Similarly, the average VD within either SMF or TMF was also not significantly different. However, overall, TMF had significantly larger VD and LA but smaller SL than SMF. Vein density was positively correlated with SD across SMF species, with a weaker correlation for TMF species and all species combined. Stomatal length was negatively correlated with SD and VD across all species. Our results extend the leaf water balance theory to diverse angiosperm tree species, and indicate decoupled adaptation of SD and VD in these species along a large altitudinal gradient.

scholarly journals A Deep Learning-Based Method for Automatic Assessment of Stomatal Index in Wheat Microscopic Images of Leaf Epidermis

2021 ◽  
Vol 12 ◽  
Author(s):  
Chuancheng Zhu ◽  
Yusong Hu ◽  
Hude Mao ◽  
Shumin Li ◽  
Fangfang Li ◽  
...  
Keyword(s):  
Deep Learning ◽  
Stomatal Density ◽  
Automatic Measurement ◽  
Epidermal Cells ◽  
Leaf Epidermis ◽  
Stomatal Index ◽  
Automatic Assessment ◽  
Microscopic Images ◽  
Index Measurement ◽  
Diagnostic Characteristics

The stomatal index of the leaf is the ratio of the number of stomata to the total number of stomata and epidermal cells. Comparing with the stomatal density, the stomatal index is relatively constant in environmental conditions and the age of the leaf and, therefore, of diagnostic characteristics for a given genotype or species. Traditional assessment methods involve manual counting of the number of stomata and epidermal cells in microphotographs, which is labor-intensive and time-consuming. Although several automatic measurement algorithms of stomatal density have been proposed, no stomatal index pipelines are currently available. The main aim of this research is to develop an automated stomatal index measurement pipeline. The proposed method employed Faster regions with convolutional neural networks (R-CNN) and U-Net and image-processing techniques to count stomata and epidermal cells, and subsequently calculate the stomatal index. To improve the labeling speed, a semi-automatic strategy was employed for epidermal cell annotation in each micrograph. Benchmarking the pipeline on 1,000 microscopic images of leaf epidermis in the wheat dataset (Triticum aestivum L.), the average counting accuracies of 98.03 and 95.03% for stomata and epidermal cells, respectively, and the final measurement accuracy of the stomatal index of 95.35% was achieved. R2 values between automatic and manual measurement of stomata, epidermal cells, and stomatal index were 0.995, 0.983, and 0.895, respectively. The average running time (ART) for the entire pipeline could be as short as 0.32 s per microphotograph. The proposed pipeline also achieved a good transferability on the other families of the plant using transfer learning, with the mean counting accuracies of 94.36 and 91.13% for stomata and epidermal cells and the stomatal index accuracy of 89.38% in seven families of the plant. The pipeline is an automatic, rapid, and accurate tool for the stomatal index measurement, enabling high-throughput phenotyping, and facilitating further understanding of the stomatal and epidermal development for the plant physiology community. To the best of our knowledge, this is the first deep learning-based microphotograph analysis pipeline for stomatal index assessment.

scholarly journals Variation in Anatomical Characteristics in Leaves of Pecan Seedstocks from Mexico and the United States

2011 ◽  
Vol 136 (2) ◽  
pp. 103-108 ◽  
Author(s):  
Madhulika Sagaram ◽  
Leonardo Lombardini ◽  
L.J. Grauke
Keyword(s):  
East Coast ◽  
Stomatal Density ◽  
The United States ◽  
Carya Illinoinensis ◽  
Annual Precipitation ◽  
Stomatal Index ◽  
Longitudinal Gradient ◽  
Water Losses ◽  
Single Location ◽  
Natural Range

Leaf anatomical traits of Mexican and U.S. pecan [Carya illinoinensis (Wangenh.) K. Koch] seedstocks grown in a single location were studied to determine patterns of ecogeographic variation within the natural range. Stomatal density was uniform among open-pollinated seedlings of a common maternal parent with twofold differences in stomatal density separating some seedstocks. There was an inverse relationship between stomatal density and epidermal cell density. Stomatal density and stomatal index of Mexican seedstocks were related to longitude and annual precipitation of origin. Stomatal density increased along the longitudinal gradient toward the east coast of Mexico; seedstocks originating from areas on the east coast of Mexico had greater stomatal density than seedstocks originating from the drier areas on the west coast. Stomatal density and stomatal index did not follow a pattern along latitude or longitude in the U.S. seedstocks. Although isotopic carbon (13C) discrimination did not vary greatly in Mexican seedstocks, the reduction in stomatal density in pecan trees from areas with reduced annual precipitation suggest the presence of an anatomical feature to reduce water losses.

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