scholarly journals The Viability of anaerobic bacteria from beef cattle feces in liquid media

2018 ◽  
Vol 1 (1) ◽  
pp. 24-30
Author(s):  
Yuli Astuti Hidayati ◽  
◽  
Tubagus Benito Ahmad Kurnani ◽  
Eulis Tanti Marlina ◽  
Khairunnisa Nur Rahmah ◽  
...  
Keyword(s):  
Beef Cattle ◽  
Liquid Medium ◽  
Biogas Production ◽  
Anaerobic Bacteria ◽  
High Survival ◽  
Liquid Media ◽  
Cattle Feces ◽  
In The Beginning

The purpose of this paper is to know the viability of anaerobic bacteria from beef cattle feces in liquid medium incubated at temperature 25oC and 39oC aims to apply as biogas starter. The research was done by the explorative method and the obtained data was analyzed descriptively. The anaerobic bacteria were grown in 98-5 medium and incubated at temperature 25oC and 39oC, observed for 2 months and the analysis was done weekly. The parameters observed were a number of anaerobic bacteria, biogas volume, and percentage of biogas. The results showed that at the beginning of incubation at temperature 25oC, the number of anaerobic bacteria 1,250 x 1010 cfu/mL, after incubating for 2 months, the number of anaerobic bacteria tend to decrease accumulatively reached 230x1010 cfu/mL, the volume of biogas production was 6 mL, the percentages of biogas production were CH4 = 0.0993%, CO2 = 1.1287%, N2 and O2 = 86.163%. At temperature 39oC, the number of anaerobic bacteria, in the beginning, accumulatively reached 585 x 1010 cfu/mL, after incubating for 2 months, the number of anaerobic bacteria decreased accumulatively reached 180x1010 cfu/mL, the volume of biogas production was 4 mL, the percentages of biogas production were CH4 = 0.134%, CO2 = 2.4714%, N2 and O2 = 89.4961%. It was concluded that an anaerobic bacterial of beef cattle feces incubated at temperature 39oC in a liquid medium generated a high survival and turn to be highly potential as a starter of biogas.

scholarly journals The Viability of anaerobic bacteria from beef cattle feces in liquid media

2018 ◽  
Vol 1 (1) ◽  
pp. 24
Author(s):  
Yuli Astuti Hidayati ◽  
Tubagus Achmad Kurnani ◽  
Eulis Tanti Marlina ◽  
Khairunnisa Nur Rahmah ◽  
Ellin Harlia
Keyword(s):  
Beef Cattle ◽  
Liquid Medium ◽  
Biogas Production ◽  
Anaerobic Bacteria ◽  
High Survival ◽  
Liquid Media ◽  
Cattle Feces ◽  
In The Beginning

The purpose of this paper is to know the viability of anaerobic bacteria from beef cattle feces in liquid medium incubated at temperature 25oC and 39oC aims to apply as biogas starter. The research was done by the explorative method and the obtained data was analyzed descriptively. The anaerobic bacteria were grown in 98-5 medium and incubated at temperature 25oC and 39oC, observed for 2 months and the analysis was done weekly. The parameters observed were a number of anaerobic bacteria, biogas volume, and percentage of biogas. The results showed that at the beginning of incubation at temperature 25oC, the number of anaerobic bacteria 1,250 x 1010 cfu/mL, after incubating for 2 months, the number of anaerobic bacteria tend to decrease accumulatively reached 230x1010 cfu/mL, the volume of biogas production was 6 mL, the percentages of biogas production were CH4 = 0.0993%, CO2 = 1.1287%, N2, and O2 = 86.163%. At temperature 39oC, the number of anaerobic bacteria, in the beginning, accumulatively reached 585 x 1010 cfu/mL, after incubating for 2 months, the number of anaerobic bacteria decreased accumulatively reached 180x1010 cfu/mL, the volume of biogas production was 4 mL, the percentages of biogas production were CH4 = 0.134%, CO2 = 2.4714%, N2, and O2 = 89.4961%. It was concluded that an anaerobic bacterial of beef cattle feces incubated at temperature 39oC in a liquid medium generated a high survival and turn to be highly potential as a starter of biogas.

Biogas production from brewery spent grain enhanced by bioaugmentation with hydrolytic anaerobic bacteria

2015 ◽  
Vol 186 ◽  
pp. 261-269 ◽  
Author(s):  
Maša Čater ◽  
Lijana Fanedl ◽  
Špela Malovrh ◽  
Romana Marinšek Logar
Keyword(s):  
Biogas Production ◽  
Anaerobic Bacteria ◽  
Spent Grain

scholarly journals The potential of various livestock waste as sources of methanogenic bacteria

2018 ◽  
Vol 1 (1) ◽  
pp. 19-23
Author(s):  
Eulis Tanti ◽  
◽  
Tubagus Benito Ahmad Kurnani ◽  
Yuli Astuti Hidayati ◽  
Khairunnisa Nur Rahmah ◽  
...  
Keyword(s):  
Beef Cattle ◽  
Dairy Cattle ◽  
Anaerobic Bacteria ◽  
Methanogenic Bacteria ◽  
Plate Count ◽  
Limiting Factor ◽  
Livestock Waste ◽  
Titration Method ◽  
Total Plate Count ◽  
The Media

Livestock waste contains organic matter as nutrients for methanogenic bacteria. However, the limiting factor was the acidogens and acetogens continuously produce compounds that reduce the pH of the system below the preferred range for methanogens to survive if sufficient buffering capacity is not available.The objective of this research is to determine the potency of various livestock waste such as dairy cattle waste and beef cattle waste, as a source of methanogens. The technique for counting bacteria was through Total Plate Count in anaerobic Hungate tube. The incubation period was 30 days. Methane was observed using Gas Chromatography (GC) analysis and the concentration of VFAs was measured using titration method. The results showed that methane production was directly proportional to the growth of anaerobic bacteria. Based on the growth of anaerobic bacteria and the production of methane in the media indicated that the dairy cattle waste and beef cattle waste were potential as a source of methanogenic bacteria.

scholarly journals Biogas Production from Anaerobic Digestion of Biodegradable Household Wastes

1970 ◽  
Vol 11 ◽  
pp. 167-172 ◽  
Author(s):  
Harka Man Lungkhimba ◽  
Amrit Bahadur Karki ◽  
Jagan Nath Shrestha
Keyword(s):  
Alternative Energy ◽  
Biogas Production ◽  
Gas Production ◽  
Biogas Plant ◽  
Waste Materials ◽  
Household Level ◽  
Fertilizer Value ◽  
Average Maximum ◽  
Cellulosic Waste ◽  
In The Beginning

This study focuses on production of biogas as an alternative energy by using biodegradable wastes (BWs) in view of solving waste management at household level. The research was conducted on ARTI model compact biogas plant of 1 m3 digester and 0.75 m3 gasholder in focusing the management of daily collected biodegradable wastes (1-2 kg) produced from households. Both laboratory and field analyses were carried out. Methane content in biogas was determined by Biogas Analyzer Gas Board-3200P. Average maximum of about 235 l gas was recorded per day with corresponding to 65 min/day burning hour with the gas flame of energy value 1.55 MJ/h. According to the plant owners, the burning period of the gas was approximately 2 h/day during the spring and pre-monsoon seasons. The average gas production (per kg) from fresh waste materials was approximately 60 l. The use of high moisture containing cellulosic waste materials and incomplete digestion due to lower digester temperature were the major causes of lower gas yield. The proportion of methane exceeded by carbondioxide in the beginning but then after gradually methane exceeded carbondioxide and reached 56.43% on an average. Although fertilizer value in terms of NPK increased gradually but it remained below 1% except K, which was found to be 1.22%. Simple payback periods of 4.81, 7.57 and 7.20 years were found in kerosene, firewood, and LPG substitutions respectively. Key words: alternative energy; biogas analyzer; compact biogas plant; methane; simple payback period DOI: 10.3126/njst.v11i0.4140Nepal Journal of Science and Technology 11 (2010) 167-172

The valuation of malnutrition in the mono-digestion of maize silage by anaerobic batch tests

2008 ◽  
Vol 58 (7) ◽  
pp. 1453-1459 ◽  
Author(s):  
L. Hinken ◽  
I. Urban ◽  
E. Haun ◽  
I. Urban ◽  
D. Weichgrebe ◽  
...  
Keyword(s):  
Trace Elements ◽  
Anaerobic Digestion ◽  
Trace Element ◽  
Biogas Production ◽  
Renewable Energy Sources ◽  
Anaerobic Bacteria ◽  
Trace Element Content ◽  
Energy Crops ◽  
Maize Silage ◽  
Batch Tests

Anaerobic digestion is a technology which is used to produce methane from organic solids and energy crops. Especially in recent years, the fermentation of energy crops has become more and more important because of increasing costs for energy and special benefits for renewable energy sources in Germany. Anaerobic bacteria require macro and micro nutrients to grow. Absence of these elements can inhibit the anaerobic process significantly. In particular mono-substrates like maize or certain industrial wastewater often cannot provide all required nutrients. For this reason this research investigates the influence of substrate and trace elements on anaerobic digestion in detail. Different agricultural anaerobic biomasses are analysed with special regard to their trace element content. Based on these results, the influence of three trace elements (iron, cobalt, and nickel) on anaerobic digestion was studied in anaerobic batch tests at different sludge loading rates and for different substrates (maize and acetate). Biogas production was found to be 35% for maize silage and up to 70% higher for acetate with trace element dosage than in the reference reactor.

scholarly journals Origin of Contamination and Genetic Diversity of Escherichia coli in Beef Cattle

2003 ◽  
Vol 69 (5) ◽  
pp. 2794-2799 ◽  
Author(s):  
Mueen Aslam ◽  
Frances Nattress ◽  
Gordon Greer ◽  
Chris Yost ◽  
Colin Gill ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Genetic Diversity ◽  
Beef Cattle ◽  
Ground Beef ◽  
Rflp Analysis ◽  
E Coli ◽  
Cattle Feces ◽  
Genetic Subtypes ◽  
Random Amplification ◽  
Pcr Rflp

ABSTRACT The possible origin of beef contamination and genetic diversity of Escherichia coli populations in beef cattle, on carcasses and ground beef, was examined by using random amplification of polymorphic DNA (RAPD) and PCR-restriction fragment length polymorphism (PCR-RFLP) analysis of the fliC gene. E. coli was recovered from the feces of 10 beef cattle during pasture grazing and feedlot finishing and from hides, carcasses, and ground beef after slaughter. The 1,403 E. coli isolates (855 fecal, 320 hide, 153 carcass, and 75 ground beef) were grouped into 121 genetic subtypes by using the RAPD method. Some of the genetic subtypes in cattle feces were also recovered from hides, prechilled carcasses, chilled carcasses, and ground beef. E. coli genetic subtypes were shared among cattle at all sample times, but a number of transient types were unique to individual animals. The genetic diversity of the E. coli population changed over time within individual animals grazing on pasture and in the feedlot. Isolates from one animal (59 fecal, 30 hide, 19 carcass, and 12 ground beef) were characterized by the PCR-RFLP analysis of the fliC gene and were grouped into eight genotypes. There was good agreement between the results obtained with the RAPD and PCR-RFLP techniques. In conclusion, the E. coli contaminating meat can originate from cattle feces, and the E. coli population in beef cattle was highly diverse. Also, genetic subtypes can be shared among animals or can be unique to an animal, and they are constantly changing.

Prevalence of Shiga Toxin–Producing Escherichia coli in Beef Cattle

2005 ◽  
Vol 68 (10) ◽  
pp. 2224-2241 ◽  
Author(s):  
HUSSEIN S. HUSSEIN ◽  
LAURIE M. BOLLINGER
Keyword(s):  
Escherichia Coli ◽  
Beef Cattle ◽  
Hemolytic Uremic Syndrome ◽  
Health Risks ◽  
Shiga Toxin ◽  
Beef Industry ◽  
Potential Health ◽  
Cattle Feces ◽  
Beef Products ◽  
Uremic Syndrome

A large number of Shiga toxin–producing Escherichia coli (STEC) strains have caused major outbreaks and sporadic cases of human illnesses, including mild diarrhea, bloody diarrhea, hemorrhagic colitis, and the life-threatening hemolytic uremic syndrome. These illnesses have been traced to both O157 and non-O157 STEC. In a large number of STEC-associated outbreaks, the infections were attributed to consumption of ground beef or other beef products contaminated with cattle feces. Thus, beef cattle are considered reservoirs of STEC and can pose significant health risks to humans. The global nature of the human food supply suggests that safety concerns with beef will continue and the challenges facing the beef industry will increase at the production and processing levels. To be prepared to address these concerns and challenges, it is critical to assess the role of beef cattle in human STEC infections. In this review, published reports on STEC in beef cattle were evaluated to achieve the following specific objectives: (i) assess the prevalence of STEC in beef cattle, and (ii) determine the potential health risks of STEC strains from beef cattle. The latter objective is critically important because many beef STEC isolates are highly virulent. Global testing of beef cattle feces revealed wide ranges of prevalence rates for O157 STEC (i.e., 0.2 to 27.8%) and non-O157 STEC (i.e., 2.1 to 70.1%). Of the 261 STEC serotypes found in beef cattle, 44 cause hemolytic uremic syndrome and 37 cause other illnesses.

scholarly journals Ecological Diversity of Microbial Consortium Feces of Beef Cattle and Lignite Coal

2020 ◽  
Vol 27 (3) ◽  
pp. 180
Author(s):  
Gina Chynthia Kamarudin Puteri ◽  
Roni Ridwan ◽  
Ellin Harlia
Keyword(s):  
Renewable Energy ◽  
Beef Cattle ◽  
Microbial Consortium ◽  
Rumen Fluid ◽  
Renewable Energy Sources ◽  
Coal Waste ◽  
Ecological Diversity ◽  
Fluid Medium ◽  
Cattle Feces ◽  
Lignite Coal

Increasing energy demand is not alongside the availability of limited fossil fuels. Alternative and renewable energy sources are not only an option to overcome energy problems but also essential to minimize global warming. Another critical and promising renewable energy source is biomass-derived from livestock feces. Beef cattle feces contain a microorganism consortium that can be used as a starter with coal media to form biogas. Indonesia recently developed coal waste processing into renewable energy, such as biogas. This study aimed to overview the ecological diversity of microbial consortium of beef cattle feces, lignite coal waste, and a combination of livestock and lignite coal waste under mesophilic conditions. This research is an explorative method, the data obtained were analyzed descriptively. The process of formation was carried out anaerobically on a bottle containing the rumen fluid medium. The fermentation process lasted 42 days at 39℃ of temperature. After that, the sample was electrophoresis, followed by next-generation sequencing (NGS) method. NGS data is processed with the MG-Rast website. This study demonstrates the ecological diversity of microbial consortium of beef cattle, lignite coal waste, and a combined consortium. The results showed ecological diversity in the form of taxonomy dominated by bacteria, eukaryotes, and archaea.

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