Engineering of Papaya Simplicia Nanoparticles for Grouper Fish (Epinephelus fuscoguttatus lanceolatus) feed

Fish growth descriptions constitute the parameters which are influenced by internal and external condition. The external factors include water quality, feed quality and feed quantity. Feed quality including the simpicia papaya particle size which contains protease enzyme and saponin as additional ingredients for grouper feed. Currently, grouper’s growth is relatively low, when in fact the protein content of its feed is high. This research aims at obtaining data on charachter particle of papaya simplicia powder and the grouper’s absolute weight and grouper’s protein efficiency ratio which is fed with simplicia papaya powder. The research is conducted using Completely Randomized Design with 5 treatments and 3 repetitions (A: 60 μm simplicia papaya 3.5% application, B: 100 nm simplicia papaya 3.5% application, C: 60 μm simplicia papaya 2% application, D: 100 nm simplicia papaya 2% application, and E: 0% without simplicia papaya). Simplicia papaya powder was made from dried papaya, then mashed using disc mill and filtered with sieves measuring 60 μm and 100nm and the grouper cultivation is done in a Floating Net Cage in Pangandaran Regency. The addition of papaya simplicia at A and B to the grouper’s feed has some highest average daily gain by 3.70 % and 3.73 %. The characteristics of simplisia papaya powder size 60 μm and 100 nm do not have a different effect on the growth performance of grouper seeds.

Biological Activity of Ethanolic Leaves Extract Lantana camara Against Cabbage Catterpillars for Environmentally Biopesticides

Chemical insecticides that have been relied upon to control cabbage catterpillars have become the main cause of pest resistance, high residues in crop products and decreased biodiversity of natural enemies in the environment. Active ingredients of plants plays an important role in providing opportunities for the development of a plant extracts, particularly applied for agriculture using the organic extract as bioinsecticide. This paper reports the ethanolic leaves extraction of L. camara against the C. pavonana and S. litura larvae which are the main pest on cabbage plants in Indonesia. The objective of this research is to evaluate the antifidan performance of ethanolic leaves extract to C. pavonana and S. litura larvae. The extract was prepared by maceration and evaporation and followed by antifeedant bioassay test. The antifeedant test was used leaf disc deep method with choice and no choice test to find out the effective minimum concentration on feeding detterent activity. The mean leaf area consumed were analyzed by using non parametric statistics of Mann-Whitney U. The results showed that the antifeedant minimum effective concentration of ethanolic leaves extract L. camara to C. pavonana larvae at 2000 ppm (choice test) and 1000 ppm (no choice test) in consider as medium antifeedant categori, to S. litura larvae at 500 ppm (choice test) and performance antifeedant in consider as medium antifeedant categori.

Additions concentration of encapsulant on biang clone purple sweet potatoes "chips" as natural dye powder

The purpose of this study was to obtain maltodextrin with the appropriate concentration of purple sweet potato anthocyanin extract to produce the natural dye powder preparations with the best physical and chemical characteristics. The research method used is Experimental Method (Experimental Method) by using Group Random Design (GRD). The experiment consisted of three treatments and each was repeated four times, with the treatment obtained in cassava purple with a concentration of 35.4 mg / 100gr anthocyanin extract. The next stage was to determine the best concentration of maltodextrin Consisting of four treatments and each repeated three times. The anthocyanin pigment powder of purple sweet potato with various treatments of maltodextrin concentration addition gave a significant effect on the soluble time, and yield, but did not gave a significant effect on total of anthocyanin, hygroscopicity and solubility. The treatment 10% of maltodextrin concentration addition resulted in best characteristic with total anthocyanin of 48.43 mg / L, color intensity L * (brightness) of 37.86, a * (redness) of 43.66, b * (yellow) of 21.68, water content of 5.56%, hygroscopicity of 11.62%, solubility of 97.13%, soluble time of 159 seconds, pH value of 3.04, and yield of 31.38%. The resulting anthocyanin powder shows that the anthocyanin pigment powder of purple sweet potato with the maltodextrin concentration addition has the potential to serve as a natural dye powder for food and beverages.

Engineering of Papaya Simplicia Powder in different size for Grouper Fish (Epinephelus fuscoguttatus lanceolatus) feed

Fish growth descriptions constitute the parameters which are influenced by internal and external condition. The external factors include water quality, feed quality and feed quantity. Feed quality including the simpicia papaya particle size which contains protease enzyme and saponin as additional ingredients for grouper feed. Currently, grouper’s growth is relatively low, when in fact the protein content of its feed is high. This research aims at obtaining data on charachter particle of papaya simplicia powder and the grouper’s absolute weight and grouper’s protein efficiency ratio which is fed with simplicia papaya powder. The research is conducted using Completely Randomized Design with 5 treatments and 3 repetitions (A: 60 μm simplicia papaya 3.5% application, B: 100 nm simplicia papaya 3.5% application, C: 60 μm simplicia papaya 2% application, D: 100 nm simplicia papaya 2% application, and E: 0% without simplicia papaya). Simplicia papaya powder was made from dried papaya, then mashed using disc mill and filtered with sieves measuring 60 μm and 100nm and the grouper cultivation is done in a Floating Net Cage in Pangandaran Regency. The addition of papaya simplicia at A and B to the grouper’s feed has some highest average daily gain by 3.70 % and 3.73 %. The characteristics of simplisia papaya powder size 60 μm and 100 nm do not have a different effect on the growth performance of grouper seeds.

Ability of Trichoderma harzianum in the Formulation with Carbon Fiber and Silica Nano Particles to Control Damping Off (Sclerotium rolfsii) on Soybean Plants

Sclerotium rolfsii is one of the soil-borne pathogens that cause damping-off and stem rot on soybean plants. One effort to control damping-off, which is environmentally friendly, is by using biological agents. Antagonistic microorganism that has been studied intensively and has a great potential to control soil-borne diseases is Trichoderma harzianum. The objectives of this research were to comprehend the ability of T. harzianum in a formulation with carbon fiber 80 mesh and silica nanoparticles (NPs.) and to determine the concentration of silica NPs. in the formulation that suppresses the in vitro growth of S. rolfsii and control the damping-off on soybean plants. The experiment was conducted in two phases. The first phase was in vitro experiment, arranged in a completely randomized design with 11 treatments and 3 replications. The second phase was in vivo test by using randomized complete block design, with 11 treatments and 3 replications. The in-vitro test showed that each treatment with T. harzianum in the formulation of silica NPs. and carbon fiber 80 mesh in various concentrations was able to suppress the S. rolfsii growth by 58.76- 80.92%. The treatment of single T. harzianum caused the highest suppression on S. rolfsii up to 80.92%. While the results of the in vivo test showed that the highest percentage of damping-off suppression was on the treatment of T. harzianum only, with 60% suppression.

Additions Concentration of Encapsulant on Biang Clone Purple Sweet Potatoes "Chips" As Natural Dye Powder

The purpose of this study was to obtain maltodextrin with the appropriate concentration of purple sweet potato anthocyanin extract to produce natural dye powder preparations with the best physical and chemical characteristics. The research method used is Experimental Method) by using Group Random Design (GRD). The experiment consisted of three treatments and each was repeated four times, with the treatment obtained in cassava purple with a concentration of 35.4 mg/100gr anthocyanin extract. The next stage was to determine the best concentration of maltodextrin Consisting of four treatments and each repeated three times. The anthocyanin pigment powder of purple sweet potato with various treatments of maltodextrin concentration addition gave a significant effect on the soluble time, and yield, but did not give a significant effect on the total of anthocyanin, hygroscopicity and solubility. The treatment 10% of maltodextrin concentration addition resulted in best characteristic with total anthocyanin of 48.43 mg / L, color intensity L* (brightness) of 37.86, a* (redness) of 43.66, b* (yellow) of 21.68, water content of 5.56%, hygroscopicity of 11.62%, solubility of 97.13%, soluble time of 159 seconds, pH value of 3.04, and yield of 31.38%. The resulting anthocyanin powder shows that the anthocyanin pigment powder of purple sweet potato with the maltodextrin concentration addition has the potential to serve as a natural dye powder for food and beverages.

The Viability of anaerobic bacteria from beef cattle feces in liquid media

The purpose of this paper is to know the viability of anaerobic bacteria from beef cattle feces in liquid medium incubated at temperature 25oC and 39oC aims to apply as biogas starter. The research was done by the explorative method and the obtained data was analyzed descriptively. The anaerobic bacteria were grown in 98-5 medium and incubated at temperature 25oC and 39oC, observed for 2 months and the analysis was done weekly. The parameters observed were a number of anaerobic bacteria, biogas volume, and percentage of biogas. The results showed that at the beginning of incubation at temperature 25oC, the number of anaerobic bacteria 1,250 x 1010 cfu/mL, after incubating for 2 months, the number of anaerobic bacteria tend to decrease accumulatively reached 230x1010 cfu/mL, the volume of biogas production was 6 mL, the percentages of biogas production were CH4 = 0.0993%, CO2 = 1.1287%, N2, and O2 = 86.163%. At temperature 39oC, the number of anaerobic bacteria, in the beginning, accumulatively reached 585 x 1010 cfu/mL, after incubating for 2 months, the number of anaerobic bacteria decreased accumulatively reached 180x1010 cfu/mL, the volume of biogas production was 4 mL, the percentages of biogas production were CH4 = 0.134%, CO2 = 2.4714%, N2, and O2 = 89.4961%. It was concluded that an anaerobic bacterial of beef cattle feces incubated at temperature 39oC in a liquid medium generated a high survival and turn to be highly potential as a starter of biogas.

Ultraviolet and violet-blue emission of Zn doped MgO/mg(OH)2 particles prepared by one step spray pyrolysis method

Spherical particles of Zn doped MgO were prepared by one-step spray pyrolysis method. The crystalline nature and particle size of the samples were characterized by X-ray diffraction analysis (XRD). The morphology of samples was studied by scanning electron microscope (SEM) and the presence of Zn in the sample was confirmed by energy dispersive X-ray analysis (EDX). The optical properties of the samples were investigated using photoluminescence spectroscopy (PL) analysis to obtain excitation and emission spectra of the samples. Results indicated that the doped MgO particles exhibited a cubic structure without hexagonal wurtzite structure as the Zn concentrations were increased. Spherical shape and porous particles are found with increasing of doping concentration. The optical band gap of MgO altered with the addition of doping concentration. A considerable redshift of about ~0.08 – 0.13 eV in the excitation spectra of 2.22 eV emission band was revealed in Zn doped MgO samples. It was highlighted that Zn doped MgO prepared by the spray pyrolysis generated emission at UV-Vis wavelength required for many applications.

The potential of various livestock waste as sources of methanogenic bacteria

Livestock waste contains organic matter as nutrients for methanogenic bacteria. However, the limiting factor was the acidogens and acetogens continuously produce compounds that reduce the pH of the system below the preferred range for methanogens to survive if sufficient buffering capacity is not available.The objective of this research is to determine the potency of various livestock waste such as dairy cattle waste and beef cattle waste, as a source of methanogens. The technique for counting bacteria was through Total Plate Count in anaerobic Hungate tube. The incubation period was 30 days. Methane was observed using Gas Chromatography (GC) analysis and the concentration of VFAs was measured using titration method. The results showed that methane production was directly proportional to the growth of anaerobic bacteria. Based on the growth of anaerobic bacteria and the production of methane in the media indicated that the dairy cattle waste and beef cattle waste were potential as a source of methanogenic bacteria.

Characteristics of sweet potatoes flour used as emergency food based on the type of varieties and the duration of fermentation

Sweet potato breeding at the Padjadjaran University farmland has produced new clones of sweet potatoes, Awachy 5 and Biang Varieties. Both clones contain high starch content, 25.46% and 15.96% respectively, having a good potential to be processed into flour as raw material for emergency food. However, due to poorly functional and amylograph characteristics, the clones need to be modified, one of which through fermentation. The objective of the study was to select the best varieties type of sweet potatoes and to determine the appropriate duration of fermentation to produce fermented sweet potatoes with the best physical, chemical and amylograph characteristics. The research method used was Randomized Block Design, consisting of 8 treatments and 3 repetitions. Awachy 5 and Biang Varieties were fermented for 0, 24, 48 and 72 hours respectively. The results showed that the fermented sweet potatoes flour of Awachy 5 with 72 hours of fermentation have produced the best characteristics of physical, chemical and amylograph, 7.21 ml/g swelling volume, 8.4% solubility, 84.37oC initial gelatinization temperature, 5092 cP peak viscosity, 2471 cP breakdown viscosity, 1089 cP setback viscosity, 68.04% starch content and 3.51% water content.