scholarly journals Cytotoxicity assessment of the stem bark of Tieghemella heckelii Pierre ex. A Chev. (Sapotaceae) towards Vero and RD human cancer Cell Lines

2017 ◽  
Vol 6 (1) ◽  
pp. 11-19
Author(s):  
Bertin Kipré Guédé ◽  
◽  
Aya Nathalie Guessennd Kouadio ◽  
Jules N’guessan. Kouadio ◽  
Mamidou Witabouna Koné ◽  
...  
Keyword(s):  
Mortality Rate ◽  
Cell Viability ◽  
Vero Cell ◽  
Cell Lines ◽  
Cancer Cell ◽  
Methanol Extract ◽  
Ethanol Extract ◽  
Stem Bark ◽  
Cancer Cell Lines ◽  
Vero Cell Lines

The present study aimed at investigating the cytotoxicity of the stem bark of Tieghemella heckelii Pierre ex. A Chev (Sapotaceae). For this purpose, plant extracts were put into contact separately with the different Vero and human RD cancer cell lines diluted in a 96 wells microplate after 24-hour incubation at 37°C. Thereafter, the absorbance was measured every 24 hour for two days, using Elisa reader spectrophotometer. The IC50 values obtained from multi-dose testing of ethanol extract against Vero cell lines ranged from 0.051 to 0.192 mg/mL. The results also showed Vero cell viability of 80.2%, and a mortality rate of 94.9% against RD cell lines whereas methanol extract displayed for the same experiment an IC50 ranging from 0.018 to 2.98 mg/mL with a cell viability of 67%, and a mortality rate of 95.6%. From these results, it could be concluded that the methanol extract of the stem bark showed higher cytotoxic activity towards RD cell lines. As for the ethanol extract, it showed significant non-cytotoxicity towards the Vero cell lines. In the light of this evidence, it can be claimed that the plant exhibited non-cytotoxic patterns against Vero cells and has anticancer potential.

scholarly journals The cytotoxicity of mekai (Albertisia papuana Becc.) root extract on breast cancer cell lines T47D and Vero cell lines

2016 ◽  
Author(s):  
Elizabeth Betty Elok Kristiani ◽  
Laurentius Hartanto Nugroho ◽  
Soekarti Moeljopawiro ◽  
Sitarina Widyarini
Keyword(s):  
Breast Cancer ◽  
Vero Cell ◽  
Cell Lines ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Cancer Cell Lines ◽  
Breast Cancer Cell Lines ◽  
Root Extract ◽  
Vero Cell Lines

scholarly journals Cytotoxic activity of casearborin c isolated from Casearia corymbosa

2019 ◽  
Vol 62 (3) ◽  
Author(s):  
María Leonor Vila-Luna ◽  
Rosa Esther Moo-Puc ◽  
Luis Wiliunfo Torres-Tapia ◽  
Sergio Rubén Peraza-Sánchez
Keyword(s):  
Cytotoxic Activity ◽  
Cell Lines ◽  
Cancer Cell ◽  
Methanol Extract ◽  
Stem Bark ◽  
Cancer Cell Lines ◽  
Syringic Acid

<p><strong>Abstract. </strong>Casearborin c (<strong>1</strong>), syringic acid (<strong>2</strong>), <em>ent</em>-3β-hydroxy-(-)-13-<em>epi</em>-manoyl oxide (<strong>3</strong>), <em>ent</em>-(-)-13-<em>epi</em>-manoyl oxide (<strong>4</strong>), <em>ent</em>-(-)-kaur-16-en-19-oic acid (<strong>5</strong>), and γ-sitosterol (<strong>6</strong>) were isolated from <em>Casearia corymbosa </em>stem bark. Only casearborin c showed cytotoxic activity on HeLa and SiHa cancer cell lines. This work contributes to the description of three compounds (<strong>1</strong>-<strong>3</strong>) newly isolated from <em>C. corymbosa </em>and highlights that casearborin c, a clerodane-type diterpene, is responsible for the cytotoxic activity shown in the original methanol extract of this species.</p><p> </p><p><strong>Resumen. </strong>Casearborina c (<strong>1</strong>), ácido siríngico (<strong>2</strong>), óxido de <em>ent</em>-3β-hidroxi-(-)-13-<em>epi</em>-manoilo (<strong>3</strong>), óxido de <em>ent</em>-(-)-13-<em>epi</em>-manoilo (<strong>4</strong>), ácido <em>ent</em>-(-)-kaur-16-en-19-oico (<strong>5</strong>) y γ-sitosterol (<strong>6</strong>) se aislaron de la corteza del tallo de <em>Casearia corymbosa</em>. Sólo casearborina c mostró actividad citotóxica en las líneas de células cancerígenas HeLa y SiHa. Este trabajo describe tres compuestos (<strong>1</strong>-<strong>3</strong>) aislados por primera vez de <em>C. corymbosa</em> y destaca que casearborina c, un diterpeno de tipo clerodano, es responsable de la actividad citotóxica del extracto metanólico de esta especie.</p>

scholarly journals Pharmaceutical immunoglobulin G impairs anti-carcinoma activity of oxaliplatin in colon cancer cells

2021 ◽  
Author(s):  
Yuru Shang ◽  
Xianbin Zhang ◽  
Lili Lu ◽  
Ke Jiang ◽  
Mathias Krohn ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Cell Viability ◽  
Cancer Patients ◽  
Cell Lines ◽  
Cancer Cell ◽  
Immunoglobulin G ◽  
Cancer Cell Lines ◽  
Human Igg ◽  
Western Blots ◽  
Igg Receptors

Abstract Background Recent evidence proves that intravenous human immunoglobulin G (IgG) can impair cancer cell viability. However, no study evaluated whether IgG application benefits cancer patients receiving chemotherapeutics. Methods Influence of pharmaceutical-grade human IgG on the viability of a series of patient-derived colon cancer cell lines with and without chemotherapeutic intervention was determined. Cell death was analysed flow cytometrically. In addition, the influence of oxaliplatin and IgG on the ERK1/2-signalling pathway was evaluated by western blots. Results We evaluated the effects of pharmaceutical IgG, such as PRIVIGEN® IgG and Tonglu® IgG, in combination with chemotherapeutics. We did not observe any significant effects of IgG on tumour cell viability directly; however, human IgG significantly impaired the anti-tumoral effects of oxaliplatin. Primary cancer cell lines express IgG receptors and accumulate human IgG intracellularly. Moreover, while oxaliplatin induced the activation of ERK1/2, the pharmaceutical IgG inhibited ERK1/2 activity. Conclusions The present study demonstrates that pharmaceutical IgG, such as PRIVIGEN® IgG and Tonglu® IgG, can impair the anti-carcinoma activity of oxaliplatin. These data strongly suggest that therapeutic IgG as co-medication might have harmful side effects in cancer patients. The clinical significance of these preclinical observations absolutely advises further preclinical, as well as epidemiological and clinical research.

scholarly journals Differential Effects of Combined ATR/WEE1 Inhibition in Cancer Cells

Cancers ◽  
2021 ◽  
Vol 13 (15) ◽  
pp. 3790
Author(s):  
Gro Elise Rødland ◽  
Sissel Hauge ◽  
Grete Hasvold ◽  
Lilli T. E. Bay ◽  
Tine T. H. Raabe ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Cell Viability ◽  
Cancer Treatment ◽  
Cancer Cells ◽  
Cell Lines ◽  
Cancer Cell ◽  
Combined Treatment ◽  
Cancer Cell Lines ◽  
Variable Extent ◽  
Synergistic Induction

Inhibitors of WEE1 and ATR kinases are considered promising for cancer treatment, either as monotherapy or in combination with chemo- or radiotherapy. Here, we addressed whether simultaneous inhibition of WEE1 and ATR might be advantageous. Effects of the WEE1 inhibitor MK1775 and ATR inhibitor VE822 were investigated in U2OS osteosarcoma cells and in four lung cancer cell lines, H460, A549, H1975, and SW900, with different sensitivities to the WEE1 inhibitor. Despite the differences in cytotoxic effects, the WEE1 inhibitor reduced the inhibitory phosphorylation of CDK, leading to increased CDK activity accompanied by ATR activation in all cell lines. However, combining ATR inhibition with WEE1 inhibition could not fully compensate for cell resistance to the WEE1 inhibitor and reduced cell viability to a variable extent. The decreased cell viability upon the combined treatment correlated with a synergistic induction of DNA damage in S-phase in U2OS cells but not in the lung cancer cells. Moreover, less synergy was found between ATR and WEE1 inhibitors upon co-treatment with radiation, suggesting that single inhibitors may be preferable together with radiotherapy. Altogether, our results support that combining WEE1 and ATR inhibitors may be beneficial for cancer treatment in some cases, but also highlight that the effects vary between cancer cell lines.

scholarly journals Screening In vitro Anticancer Activity of Alseodaphne semecarpifolia Nees Stem Bark Extracts against some Cancer Cell lines

2019 ◽  
Vol 11 (5) ◽  
pp. 884-888
Author(s):  
Chethankumara Ganadhal Puttaramaiah ◽  
Krishna Venkatarangaiah ◽  
Nagaraj Kakanahalli
Keyword(s):  
Anticancer Activity ◽  
Cell Lines ◽  
Cancer Cell ◽  
Stem Bark ◽  
Cancer Cell Lines ◽  
Screening In Vitro

scholarly journals Bio-guided Isolation of a New Sesterterpene from Serjania goniocarpa

2015 ◽  
Vol 10 (9) ◽  
pp. 1934578X1501000
Author(s):  
Carlos Quintal-Novelo ◽  
Luis W. Torres-Tapia ◽  
Rosa Moo-Puc ◽  
Sergio R. Peraza-Sanchez
Keyword(s):  
Palmitic Acid ◽  
Traditional Medicine ◽  
Cell Lines ◽  
Cancer Cell ◽  
Antiproliferative Activity ◽  
Methanol Extract ◽  
Cancer Cell Lines ◽  
Spectroscopic Analyses

Serjania goniocarpa is a plant used in Mayan traditional medicine as a remedy for the treatment of cancer-like symptoms. Bio-guided fractionation of the methanol extract of the leaves led to the isolation of an α- and β-amyrin mixture, palmitic acid, phytol and the new sesterterpene goniocarpic acid whose structure was elucidated by IR, GC-MS, and NMR spectroscopic analyses. Goniocarpic acid exhibited cytotoxic and antiproliferative activity against several cancer cell lines.

Characteristics of the combination paclitaxel plus doxorubicin in breast cancer cell lines analyzed with the ATP-Cell Viability Assay

1993 ◽  
Vol 28 (1) ◽  
pp. 21-27 ◽  
Author(s):  
Ossi R. Koechli ◽  
Bernd-Uwe Sevin ◽  
James P. Perras ◽  
Ting Chao Chou ◽  
Roberto Angioli ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cell Viability ◽  
Cell Lines ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Cancer Cell Lines ◽  
Breast Cancer Cell Lines ◽  
Cell Viability Assay ◽  
Viability Assay
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