scholarly journals The Protective Role of crude Polyphenolic Compounds Extracted from black Olive fruit (Oleaeuropae ) on Liver Functions in Males Rats Treated with Hydrogen Peroxide

2012 ◽  
Vol 36 (0E) ◽  
pp. 164-171
Author(s):  
Layla Hashim Alol
Keyword(s):  
Serum Bilirubin ◽  
Inflammatory Cells ◽  
Treated Group ◽  
Protective Role ◽  
Polyphenolic Compounds ◽  
Total Serum Bilirubin ◽  
Male Rats ◽  
Total Serum ◽  
Control Group ◽  
Black Olive

This study was conducted to investigate the protective role of polyphenolic compounds extracted from olive (Oleaeuropae) to contrast the damaging effects of 1% hydrogen peroxide on liver functions in male rats. Crude polyphenolic compounds were extracted from fruits of black olive by 95% methanolic extraction method. Twenty adult male rats (200-220gm.) were randomly divided into four equals groups and treated daily for 30 days. Rats in the first group received tap water (orally)and considered as control group, animals of second group received 1% H2O2 in drinking water . The rats in the third group received 1% H2O2 in drinking water plus 200mg/kgB.W. of crude polyphenolic compounds while animals in the fourth group received 200mg/kg B.W. of crude polyphenoliccompounds. At the end of the experiment, blood samples were taken to investigate the activity of liver enzymes (Aspartate aminotransferase (AST) and Alanine aminotransferase (ALT), concentration of total serum bilirubin, as well as protein picture of blood serum by using agarose gel electrophoresis. Ultimately, animals of all groups were sacrified to examine the histopathological changes in liver. The results illustrated significant increase (P<0.05) in liver enzymes activity (AST,ALT) and total serum bilirubin in H2O2 treated group as compared with control. Although rats treated polyphenolic compounds of olive plus H2O2 showed significant decrement (P<0.05) in ALT activity and total serum bilirubin, while no significant alteration in (AST) activity was recorded in H2O2 treated group. The result also demonstrated significant decrease (P<0.05) in authority of ALT, total serum bilirubin in animals treated with polyphenolic compounds. Serum proteins showed a significant (P<0.05) decreament of albumin percentage and increment of globulins in H2O2 treated group as well as polyphenolic compounds treated group as compared with control group (G1) . However, no significant different in group treated with polyphenolic compounds as compared with control. Histological sections of liver illustrated clear impact of group treated with H2O2, manifested by necrosis of hepatic cells with infiltration of inflammatory cells while animals treated with polyphenolic compounds plus H2O2 revealed slight infiltration of inflammatory cells with proliferation of kupffer cells in liver. In infereance, the autcomes of this study documented the advantageous effect of crude polyphenolic compounds of olive apposite the noxious effect of H2o2 on liver function of adult males rats.

scholarly journals Protective role of ceftriaxone plus sulbactam with VRP1034 on oxidative stress, hematological and enzymatic parameters in cadmium toxicity induced rat model

2012 ◽  
Vol 5 (4) ◽  
pp. 192-200 ◽  
Author(s):  
Vivek Kumar Dwivedi ◽  
Anuj Bhatanagar ◽  
Manu Chaudhary
Keyword(s):  
Free Radical ◽  
Tissue Injury ◽  
Cadmium Toxicity ◽  
Treated Group ◽  
Protective Role ◽  
Enzymatic Activities ◽  
Exposed Group ◽  
Male Rats ◽  
Control Group

ABSTRACT We investigated the protective role of ceftriaxone plus sulbactam with VRP1034 (Elores) on hematological, lipid peroxidation, antioxidant enzymatic activities and Cd levels in the blood and tissues of cadmium exposed rats. Twenty-four male rats were divided into three groups of eight rats each. The control group received distilled water whereas group II received CdCl2 (1.5 mg/4 ml/body weight) through gastric gavage for 21 days. Group III received CdCl2 and was treated with ceftriaxone plus sulbactam with VRP1034 for 21 days. The hematological, biochemical, lipid per-oxidation levels and enzymatic parameters were measured in plasma and tissues (brain, liver and kidney) of all groups. The Cd, Zn and Fe levels were measured in blood and tissues of all groups. Our findings showed significantly decreased cadmium (p<0.001), malonaldialdehyde (p<0.001) and myloperoxidase (MPO) levels along with significantly increased hemoglobin (p<0.01), RBC (p<0.05), hematocrit (p<0.05) levels and all antioxidant enzymatic activities (SOD, CAT, GR, GPx) in plasma and tissues of ceftriaxone plus sulbactam with VRP1034 treated group as compared to cadmium exposed group. Delta aminolevulinate dehydratase (δ-ALAD) activity was significantly (p<0.001) increased in the blood of ceftriaxone plus sulbactam with VRP1034 treated group as compared with cadmium exposed group. The levels of hepatic and renal parameters were significantly (p<0.001) decreased in ceftriaxone plus sulbactam with VRP1034 treated group as compared to cadmium exposed group. These findings indicate that ceftriaxone plus sulbactam with VRP1034 acts as a potent free radical scavenger and exhibits metal chelating properties that reduce free radical mediated tissue injury and prevent dysfunction of hepatic and renal organs during metal intoxication.

scholarly journals Effectiveness of phototherapy with reflecting curtains on neonatal jaundice

2011 ◽  
Vol 51 (5) ◽  
pp. 256 ◽  
Author(s):  
Ari Kurniasih ◽  
Guslihan Dasa Tjipta ◽  
Muhammad Ali ◽  
Emil Azlin ◽  
Pertin Sianturi
Keyword(s):  
Neonatal Jaundice ◽  
Serum Bilirubin ◽  
Low Cost ◽  
Total Serum Bilirubin ◽  
Spectral Irradiance ◽  
Total Serum ◽  
Control Group ◽  
Study Group ◽  
Term Newborns ◽  
The Mean

Background Although phototherapy has been used in clinical practice for 40 years, there is still much debate on how to provide the most efficacious phototherapy. Phototherapy with white reflecting curtains may increase the average spectral irradiance provided, as well as decrease serum bilirubin concentrations at a faster rate in neonates with jaundice.Objective To determine if adding low cost, white, reflecting curtains to a standard phototherapy unit can increase the effectiveness of phototherapy for neonatal jaundice.Methods A randomized, controlled, open trial was conducted at H. Adam Malik and Pirngadi Hospitals, Medan, from May to December 2009. The criteria for inclusion in the study were full term newborns with neonatal jaundice presenting in their first week of life. Single phototherapy with white curtains hanging from the sides of the phototherapy unit (study group, n=30) was compared to single phototherapy without curtains (control group, n=30). The primary outcomes measured were the mean difference in total serum bilirubin levels and average spectral irradiation levels measured at baseline, and after 12 hours and 24 hours of phototherapy.Results The sum of average spectral irradiance in the curtained phototherapy unit was significantly higher than that of the standard phototherapy unit without curtains (P < 0.05). The decrease of total serum bilirubin levels after 12 and 24 hours of phototherapy was significantly greater in the study group (3.71 and 9.7 mg/dl, respectively) than in the control group (0.1 and 3.8 mg/dl, respectively), both P <0.05.Conclusion White, reflecting curtains in phototherapy units was significantly more effective than phototherapy without curtains for treatment of neonatal jaundice. [Paediatr Indones. 2011;51:256-61].

Efficacy and Safety of Fenofibrate in Uncomplicated Hyperbilirubinemia in Newborn: A Randomized Trial, with a 6-month Follow-up

2020 ◽  
pp. 37-42
Author(s):  
Jayendra R. Gohil ◽  
Vishal S. Rathod ◽  
Bhoomika D. Rathod
Keyword(s):  
Single Dose ◽  
Serum Bilirubin ◽  
Total Serum Bilirubin ◽  
Total Serum ◽  
Control Group ◽  
Term Neonates ◽  
Significant Difference ◽  
Group A ◽  
Group B

Objective: To study the effect and safety of Fenofibrate in uncomplicated hyperbilirubinemia in newborn with 6-month follow-up. Materials and Methods: This is a randomized controlled clinical trial conducted in 60 normal term neonates admitted for uncomplicated hyperbilirubinemia in NICU at Sir T G Hospital, Bhavnagar from January 2012 to December 2012. The data included: age, sex, total serum bilirubin (TSB), weight and duration of phototherapy. All neonates enrolled in the study received phototherapy. They were divided in two groups of 30 each: control group A and group B receiving Fenofibrate (100 mg/kg single dose). There was statistically insignificant difference between the parameters of age, sex, weight and TSB between the two groups at hospitalization. Data was analyzed by using appropriate statistical methods. Results: Mean values for total serum bilirubin in Fenofibrate group B at 24 and 48 hours after admission were significantly lower than those for control group A (p<0.0001,  p=0.0001). There was no significant difference in fall of TSB between 24 and 48 hours. The mean duration of phototherapy in Fenofibrate group (44.8h: 24-72h) was significantly shorter than that in control group (55.2 h: 24‐96 h) (P=0.02). There were no side effects of the drug observed during the study and during 6 months follow up period. Conclusion: Fenofibrate as a single 100 mg/kg dose in healthy full term neonates, is effective and a safe drug (till six-month follow-up) for neonatal hyperbilirubinemia, that can decrease the time needed for phototherapy and hence hospitalization. Effect of a single dose seems to wane after 24 hours.

scholarly journals Protective Effect of Vitamins C and E against Gasoline Vapors Induced Haematological and Biochemical Changes in Male Rats

2015 ◽  
Vol 7 (3) ◽  
pp. 139-149
Author(s):  
A. Sedky ◽  
H. Elsawy
Keyword(s):  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Hemoglobin Concentration ◽  
Protective Role ◽  
Lipoprotein Cholesterol ◽  
Male Rats ◽  
Total Serum Protein ◽  
Total Serum ◽  
Control Group ◽  
Concomitant Treatment

The present work was designed to investigate the changes in some hematological, biochemical parameters and lipid profile as well as possible protective role of vitamins C and E against gasoline vapors induced toxicity in male rats. The present results showed that gasoline 80 vapors significantly decreased (p?0.05) the concentration of total serum protein (TSP) and albumin concentrations and increased (p?0.05) in serum activities of serum aminotransferases (ALT and AST) and alkaline phosphatase (ALP) compared to the control group. Also, exposure to gasoline 80 vapors induced significant decrease (p?0.05) in hemoglobin concentration, red blood cell count (RBCs), packed cell volume (PCV) and high density lipoprotein cholesterol (HDL-C) compared to control group. On the other hand, exposure to gasoline 80 vapors resulted in significant increase (p?0.05) in the levels of total cholesterol (TC), triglycerides (TGs), LDL-cholesterol (LDL-C) and very low density lipoprotein cholesterol (VLDL-C) relative to untreated experimental animals. However, concomitant treatment with gasoline vapors and administration of vitamins C and E exhibited a protective role on the observed toxic effect of gasoline vapors in male rats. The results of the present study indicated that toxic effects of gasoline vapors could be reduced by dietary supplementation of vitamins C and E.

scholarly journals Efficacy of prophylactic phototherapy for prevention of hyperbilirubinemia in very low birth weight newborns

2016 ◽  
Vol 6 (2) ◽  
pp. 151
Author(s):  
M. A. Mannan ◽  
Ismat Jahan ◽  
Sadeka Choudhury Moni ◽  
Zahidul Hasan ◽  
Arjun Chandra Dey ◽  
...  
Keyword(s):  
Clinical Trial ◽  
Birth Weight ◽  
Exchange Transfusion ◽  
Serum Bilirubin ◽  
Serum Bilirubin Level ◽  
Total Serum Bilirubin ◽  
Total Serum ◽  
Control Group ◽  
P Value ◽  
Unconjugated Hyperbilirubinemia

<p><strong>Background:</strong> Jaundice is a common clinical condition in newborn occurring in approximately 60% of term and 80% of preterm infants. Unconjugated hyperbilirubinemia is universally common in all preterm infants especially in newborns with very low biLth weight. Low birth weight and premature infants are at major risk for exaggerated hyperbilirubinemia that can lead to bilirubin encephalopathy. Significant heterogeneity in the approach to the treatment of jaundiced neonates exists throughout the world. Phototherapy is the most common treatment for neonatal hyperbilirubinemia and could be most effective in preventing the sequelae of hyperbilirubinemia if initiated prophylactically. This randomized clinical trial has been proposed with the objective of assessing the efficacy of prophylactic photo therapy in preventing significant rise of unconjugated hyperbilirubinemia in premature neonates weighing less than 1500 gram and therefore to decrease the need for exchange transfusion and finally to reduce hospital stay due to hyperbilirubinemia. <strong></strong></p><p><strong>Methods:</strong> This randomized controlled clinical trial enrolled sixty newborns with birth weight less than 1500 gram. They were divided into two groups: 1) Prophylactic group, in whom phototherapy was started within 24 hours of birth and continued for 7 days and 2) Control group in whom therapeutic phototherapy was started considering serum bilirubin level and other clinical condi­tions as per institutional guidelines. Mean value of total serum bilirubin (TSB), duration of phototherapy, the need for exchange transfusion and duration of hospital stay in both groups were analyzed.</p><p><strong>Results:</strong> The maximum mean TSB level in prophylactic group was observed on 7th day and in control group it was observed on 3rd day of life. The total serum bilirubin levels were significantly lower in the 3rd and 5th days of life in the prophylactic group in comparison to control group (P value 0.001). Total serum bilirubin level exceeded therapeutic range in 6 (21 %) and 14 (50 %) newborns of the prophylactic group and control groups respectively (P value 0.026). No documented side effects of prophylactic photo­therapy was observed.<strong> </strong></p><p><strong>Conclusion:</strong> The use of prophylactic photo therapy for infants weighing less than 1500 grn is effec­tive and sate when compared to the control group, considering satisfactory maintenance of low total serum bilimbin levels during first 7 days of life.</p>

scholarly journals Influence of crude extract of Hawthorn crataegus oxyacantha on some physiological aspects in mature male Rats exposed to hydrogen peroxide over load.

2012 ◽  
Vol 36 (1) ◽  
pp. 37-44
Author(s):  
Anwar I. Obeed Al-Abdaly
Keyword(s):  
Crude Extract ◽  
Histological Study ◽  
Oral Treatment ◽  
Inflammatory Cells ◽  
Treated Group ◽  
Mature Male ◽  
Male Rats ◽  
Control Group ◽  
Protective Effects ◽  
Alcohol Extract

This study was carried out to investigate the protective effects of 70% ethanolic alcohol extract of hawthorn (crataegus oxycantha) on some physiological functions of male rats exposed to 1% H2O2. Fifteen mature male Newzeland rats were randomly divided into three groups:- control group (C) ,two groups treated with 1% H2O2 alone (G1) or 1%H2O2 with crude extract of hawthorn(G2) orally daily for 30 days .Blood samples were taken at zero time and 30 days of the experiment .The present study declared an alteration in the lipid profile of the treated group (G2) at the end of treatment (30 days) manifested by asignificant reduction (p<0.05) in serumTC,TAG,LDL-C, VLDL-C concentrations. And elevation (p<0.05) in serum, HDL-C, as compared to the treated group (G1). Antioxidant status also exhibited significant (p<0.05) changes characterized by an elevation of serum GSH in group (G2). Histological study revealed that oral treatment with 1% H2O2 caused congestion of blood vessels of the heart with infiltration of inflammatory cells and odema between muscle fibers. It is concluded that treatment with hawthorn showed no clear pathological lesions.

scholarly journals Effect of Oral Fenofibrate on Serum Bilirubin Level in Term Neonates with Unconjugated Hyperbilirubinaemia: A Randomized Control Trial

2021 ◽  
Vol 16 (1) ◽  
pp. 35-38
Author(s):  
Murshida Mosharref ◽  
Naila Rehnuma ◽  
Nusrat Jahan ◽  
Farzana Zafreen
Keyword(s):  
Bilirubin Level ◽  
Serum Bilirubin ◽  
Serum Bilirubin Level ◽  
Total Serum Bilirubin ◽  
Military Hospital ◽  
Total Serum ◽  
Control Group ◽  
Term Neonates ◽  
Normal Birth ◽  
And Control

Introduction: Hyperbilirubinemia is a common problem in the neonatal period. Phototherapy is the most important proposed treatments for hyperbilirubinemia, but several drugs along with phototherapy are used with recent advances. Aim: To see the effect of oral fenofibrate on serum bilirubin level in term neonates with unconjugated hyperbilirubinaemia. Methods: This prospective study was carried out in Combined Military Hospital Cumilla from July 2018 to June 2019. Total 60 term and normal birth weight neonates with neonatal jaundice were enrolled in this study. Jaundiced newborns presenting with infection, G6PD deficiency, conjugated bilirubin >2 mg/dl or >15% of total serum bilirubin (TSB) and congenital anomalies were excluded from this study. These neonates were randomly allocated to the Fenofibrate group (30 cases) and Control group (30 cases). Total serum bilirubin was measured every 24 hours till the end of phototherapy and at the time of discharge. Statistical analysis was done using SPSS 22.0 and p<0.05 was considered significant. Results: There were no significant differences in gender, age, weight, gestational age and type of delivery between two groups. Mean duration of hospital stay were 4.0±0.7 and 5.5±1.4 days in Fenofibrate group and Control group respectively (p<0.001). In Fenofibrate group, TSB was decreased from 17.2 mg/dl to 15.2 mg/dl after 24 hours, to 13.6 mg/dl after 48 hours, and to 10.1 mg/dl at the time of discharge.  In control group, TSB was decreased from 17.0 mg/dl to 16.3 mg/dl after 24 hours, to 15.9 mg/dl after 48 hours, and to 10.3 mg/dl at the time of discharge. Conclusion: Treatment of neonatal unconjugated hyper- bilirubinemia with fenofibrate reduces neonatal bilirubin levels and decreases the need for phototherapy and hospitalization. JAFMC Bangladesh. Vol 15, No 1 (June) 2020: 35-38

scholarly journals Urinary N-Acetyl-beta-D-glucosaminidase as an Early Marker for Acute Kidney Injury in Full-Term Newborns with Neonatal Hyperbilirubinemia

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Bangning Cheng ◽  
Yulian Jin ◽  
Guanghui Liu ◽  
Zhiheng Chen ◽  
Hongmei Dai ◽  
...  
Keyword(s):  
Acute Kidney Injury ◽  
Serum Bilirubin ◽  
Kidney Injury ◽  
Full Term ◽  
Total Serum Bilirubin ◽  
Total Serum ◽  
Control Group ◽  
Term Newborns ◽  
Group 3 ◽  
Group 2

Purpose. To investigate renal function estimated by markers in full-term newborns with hyperbilirubinemia.Methods. A total of 332 full-term newborns with hyperbilirubinemia and 60 healthy full-term newborns were enrolled. Total serum bilirubin, serum creatinine (Cr), serum blood urea nitrogen (BUN), serum cystatin C (Cys-C), urinary beta-2-microglobulin (β2MG) index, and urinary N-acetyl-beta-D-glucosaminidase (NAG) index were measured before and after treatment. All newborns were divided into three groups according to total serum bilirubin levels: group 1 (221-256), group 2 (256-342), and group 3 (>342).Results. The control group and group 1 did not differ significantly in regard to serum Cr, serum BUN, serum Cys-C, urinaryβ2MG index, and urinary NAG index. Urinary NAG index in group 2 was significantly higher than that in control group (P<0.001). Between control group and group 3, serum Cys-C, urinaryβ2MG index, and urinary NAG index differed significantly. The significant positive correlation between total serum bilirubin and urinary NAG index was found in newborns when total serum bilirubin level was more than 272 μmol/L.Conclusions. High unconjugated bilirubin could result in acute kidney injury in full-term newborns. Urinary NAG might be the suitable marker for predicting acute kidney injury in full-term newborns with hyperbilirubinemia.

scholarly journals Possible ameliorative effects of hydromethanol extract of Thymus vulgaris on cadmium induced hepatorenal toxicity in rats

2020 ◽  
Vol 12 (3) ◽  
pp. 568-577
Author(s):  
Remigius I. ONOJA ◽  
Chinwe U. CHUKWUDI ◽  
Nnenna T. EMEJUO ◽  
Hillary E. UGWUANYI ◽  
Emmanuel U. UGWUEZE
Keyword(s):  
Oxidative Stress ◽  
Treated Group ◽  
Male Rats ◽  
Total Serum ◽  
Thymus Vulgaris ◽  
Control Group ◽  
Single Subcutaneous Dose ◽  
Subcutaneous Dose ◽  
Group D ◽  
Phosphate Buffered Saline

This study evaluated the possible ameliorative effect of hydromethanol extract of Thymus vulgaris on hepatorenal toxicity induced by cadmium in male rats. The experimental animals were divided into four groups and treated as follows: A (control - 0.5ml of 2% tween 80 in distilled water per os) for 3 weeks and a single subcutaneous dose of phosphate buffered saline, B (single subcutaneous dose of cadmium in phosphate buffered saline at 3 mg/kg); C (500 mg/kg extract per os daily for 3 weeks) and D (single subcutaneous dose of cadmium in phosphate buffered saline at 3 mg/kg + 500 mg/kg extract per os daily for 3 weeks). Cadmium administration resulted in suppression of erythrocyte count, hemoglobin concentration, packed cell volume, an elevated total leucocyte count with associated neutrophilia which improved with extract administration. Levels of serum alanine aminotransferase, aspartate aminotransferase and alkaline phosphatase, creatinine and total bilirubin concentration increased with decrease in total serum protein and albumin in cadmium treated group B compared to control group A, group C (extract only) and cadmium plus extract treated group D. Cadmium led to a reduction in catalase and superoxide dismutase activities with increase in the level of malondialdehyde. However, co-administration of extract with cadmium in group D reduced lipid peroxidation and oxidative stress induced by cadmium. Histopathological examination of cadmium treated groups showed moderate vacuolar degeneration in the liver and degeneration of the kidney tubules which were ameliorated following co-administration with extract. This study shows that Thymus vulgaris extract has a potential protective effect against cadmium induced hepato-renal injury through the suppression of oxidative stress.

Evaluation Of Some Blood Biomarkers As Predictors Of Neonatal Necrotizing Enterocolitis

QJM ◽  
2021 ◽  
Vol 114 (Supplement_1) ◽  
Author(s):  
Nehal M El-Raggal ◽  
Yasser W Darweesh ◽  
Azza M Hassan ◽  
Esraa R Zaki ◽  
Dina E Rabie
Keyword(s):  
Early Diagnosis ◽  
Necrotizing Enterocolitis ◽  
Metabolic Diseases ◽  
Serum Bilirubin ◽  
Perinatal Asphyxia ◽  
Total Serum Bilirubin ◽  
Predictive Score ◽  
Preterm Neonates ◽  
Total Serum ◽  
Control Group

Abstract Background Necrotizing enterocolitis is a leading cause of mortality and morbidity in preterm neonates; however the lack of a reliable biomarker makes definite diagnosis difficult. Therefore, its early suspicion and proper management are important. Objective To evaluate the value of NLR, serum levels GGT, total serum bilirubin and serum Ca2+ concentrations for early diagnosis and prediction of NEC severity; and to make a predictive score for early diagnosis of NEC. Patients and methods A case control study which was conducted on neonates admitted to NICU, Children Hospital, Ain Shams University over a period of 6 months, fifty neonates were classified into two groups: the patients group included 25 neonates with NEC with a gestational age 28-36 weeks regardless of birth weight matched with control group which included 25 healthy preterm neonates, however we excluded neonates with congenital infectious diseases, perinatal asphyxia, severe birth defects, congenital digestive tract malformations, inherited metabolic diseases or parental refusal of enrollment. Results ROC curve of NEC diagnosis by NLR, serum GGT, total serum bilirubin and serum Ca2+ showed cut off: &gt; 1.13, &gt; 40 U/L, &gt; 0.95 mg/dl, &lt; 8.85 mg/dl respectively, Sensitivity 84, 92%, 84%, 84% respectively and Specificity 80%, 92%, 92%, 84% respectively; also beta-weighted score for prediction of NEC disease showed that NLR (Best cut off &gt; 1.13) or GGT (Best cut off &gt; 40 U/L could be helpful with sensitivity 92% and specificity 92%. Conclusion NLR, GGT, T.Bil and calcium levels could be good non-invasive markers for NEC disease prediction and also for its diagnosis.

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