scholarly journals Induce estrus with ultrasonography examination and progesterone hormone assay for pregnancy diagnosis in Iraqi goats

2017 ◽  
Vol 40 (2) ◽  
pp. 89-93
Author(s):  
A. F. Alwan
Keyword(s):  
Serum Level ◽  
Jugular Vein ◽  
Chorionic Gonadotrophin ◽  
Progesterone Concentration ◽  
Abdominal Ultrasonography ◽  
Transabdominal Ultrasonography ◽  
Blood Samples ◽  
Pregnant Animal ◽  
Pregnancy Diagnosis ◽  
Breeding Seasons

     The aim of present work is to induce ovulation in 40 female goats in non-breeding seasons and pregnancy diagnosis using RIA of progesterone serum level and trans abdominal ultrasonography with 3.5 MHz prop. The 40 Iraqi goats were naturally inseminated during estrous phase, using fertile backs, after withdrawal of intravaginal impregnated sponges with 20 mg of cronolone               (Fluorogestone Acetate progestagen) kept for eleven days and 400 IU of equine chorionic gonadotrophin inject I/M 24hrs. before sponge withdraw. The results indicated that all does were showed (100%) estrous sign, the estrous time was 24-66 hrs. after sponge withdrawal and the pregnancy rate 67.5%. Blood samples were collected from the jugular vein before progesterone treatment, at the time of insemination and after 21 day of insemination. Early pregnancy diagnosis by RIA of progesterone concentration was performed and showed progesterone hormone value as were increasing significantly (P<0.01) from 0.26±0.12 ng/ml before insemination to 3.29±1.10 ng/ml in 21 days after insemination. Pregnant animal was examined using transabdominal ultrasonography during 45, 60 and 90 days after insemination. In conclusion, estrus could be efficiently induced in female goats during non-breeding season using 20 mg cronolone impregnated sponge and 400 IU equine chorionic gonadotrophin I/M at 24 hrs. before spongy withdrawal. The effective of early detection of pregnancy by progesterone assay in 21 days after insemination and ultrasonography by abdominal 3.5 MHz transducer after 45 days of pregnancy.

Serum Endocrine Profile in Relation to Estrus Synchronization in Surti Does

2018 ◽  
Vol 13 (03) ◽  
Author(s):  
M. M. Chaudhary ◽  
C. T. Khasatiya ◽  
S. B. Patel ◽  
S. S. Chaudhary ◽  
V. B. Atara ◽  
...  
Keyword(s):  
Jugular Vein ◽  
Estrus Synchronization ◽  
Progesterone Concentration ◽  
Serum Progesterone ◽  
Blood Samples ◽  
Group Iii ◽  
Group I ◽  
Endocrine Profile ◽  
Group Ii ◽  
And Control

The serum progesterone and estradiol profiles during synchronization of estrus by buck effect and PGF2α treatments were monitored in Surti does. Total eighteen non-pregnant does selected were evenly divided into 3 groups, 6 does in each group. The does of Group I were teased with a sexuallyactive- apronized buck; and those of Group II were treated with PGF2α, i.e., Inj. Lutalyse® @ 7.5 mg/doe IM twice 11 days apart, while the Group III served as untreated control. Blood samples were collected from all the animals on day 0 (before 1st PGF2α injection), 3rd day (during treatment), 11th day (before 2nd PGF2α injection), 14th day (after treatment) and 40th day (post-service) by jugular vein puncture. The serum separated was stored at -20°C till further analysis. In all the three groups, 83.33% does, conceived at first service in the sampling cycle. The overall mean serum progesterone concentration of Group I does (5.82±0.72 ng/ml) was significantly higher (p less than 0.01) as compared to Group II (2.93±0.38 ng/ml) and III (2.88±0.30 ng/ml). Similarly, the overall mean serum progesterone concentration of Surti does on day 0 (2.65±0.46 ng/ml), 3rd (2.56±0.80 ng/ml), 11th (4.45±0.84 ng/ml) and 14th (3.40±0.63 ng/ml) did not differ significantly, but the overall mean level at day 40 (6.31±0.45 ng/ml) was significantly (p less than 0.01) higher, because most of animals became pregnant at that time. The overall mean serum oestradiol-17β levels of Group I (24.40±2.98 pg/ ml) was significantly higher (p less than 0.01) than in Group II (15.77±1.77 pg/ml) and III (12.21±1.45 pg/ ml). On the other hand, the overall mean serum oestradiol-17β levels of Surti does on day 0 (12.89±1.21 pg/ml), 3rd (15.84±1.74 pg/ml), 11th (14.81±1.96 pg/ml), 14th (22.15±2.97 pg/ml) and 40th (21.64±5.16 pg/ml) did not differ significantly (p>0.05) and the slightly higher overall mean level found at 40th day might be the influence of the non-pregnant does at first service in the cumulative animals. The hormonal profile reflected the initiation of cyclicity and establishment of pregnancy in treated and control animals.

scholarly journals Weight and age at puberty and their correlations with morphometric measurements in crossbred breed Suffolk ewe lambs

2010 ◽  
Vol 39 (1) ◽  
pp. 134-141 ◽  
Author(s):  
Janine de Campos Ferra ◽  
Silvia Cieslak ◽  
Roberto Sartori Filho ◽  
Connie McManus ◽  
Carlos Frederico Martins ◽  
...  
Keyword(s):  
Body Length ◽  
Jugular Vein ◽  
Progesterone Concentration ◽  
Morphometric Measurements ◽  
Mato Grosso ◽  
Plasma Progesterone ◽  
Blood Samples ◽  
Ewe Lambs ◽  
Length Width ◽  
Mato Grosso Do Sul

The objective of this study was to identify and to correlate parameters that characterize puberty and estimate the age and weight of Suffolk ewe lambs in Mato Grosso do Sul. This study was carried out with twenty-two Suffolk ewe lambs for 256 days. Blood samples were collected from the jugular vein and centrifugated and the plasma were stored at -20°C until analysis of progesterone (P4) concentration by radioimmunoassay (RIA). The morphometric measurements included: head length and width, body length, elbow height, chest perimeter, back-sternal diameter, croup length, width and height, cannon bone perimeter and shoulder and hip width, using a tape. The animals became pubertal when the plasma progesterone concentration was >1.0 ng/mL, at 34.1 ± 5.2 kg and 39.5 ± 8.5 weeks of age. Meanwhile, the lambs that did not reach puberty (37.7 ± 11.4 weeks of age) were lighter. The morphometric measurements has medium to low correlations with age and weight at puberty.

The testis is not the major source of circulating follistatin in the ram

1996 ◽  
Vol 149 (1) ◽  
pp. 55-63 ◽  
Author(s):  
A J Tilbrook ◽  
D M de Kretser ◽  
F R Dunshea ◽  
R Klein ◽  
D M Robertson ◽  
...  
Keyword(s):  
Breeding Season ◽  
Jugular Vein ◽  
Plasma Concentrations ◽  
Testicular Tissue ◽  
Blood Samples ◽  
Gonadotrophin Releasing Hormone ◽  
Physiological Dose ◽  
Significant Uptake ◽  
Breeding Seasons ◽  
Matched Samples

Abstract The aims of this study were to determine the plasma concentrations of follistatin in rams and to assess if the testis contributes to circulating follistatin and if there is uptake or production of follistatin by the head in rams. Catheters were inserted in the carotid artery, jugular vein and spermatic vein of intact rams during the non-breeding season (experiment 1; n=5) and breeding season (experiment 2; n=4). In experiment 1, blood samples were collected from 5 rams every 10 min for 4 h, commencing 20–60 min after surgery. After 2 h of sampling 1 μg gonadotrophin-releasing hormone (GnRH) was injected intravenously. In experiment 2, blood samples were collected from 4 of the rams used in experiment 1 by venipuncture 30 and 15 min before surgery and every 15 min throughout surgery. Commencing 1 h after surgery, matched samples were taken from each of the vessels every 10 min for 4 h (1–4 h after surgery), then every hour for 20 h (4–24 h after surgery) and then every 10 min for 4 h (24–28 h after surgery). In both experiments, follistatin secretion was non-pulsatile and there were no significant differences between the concentrations of follistatin in any of the vessels. There was a significant (P<0·05) increase in the concentrations of follistatin in each of the vessels throughout the 4 h of 10-min sampling in both experiments. In experiment 2 plasma concentrations of follistatin in the jugular vein were significantly (P<0·05) lower before surgery than at other stages of the experiment. During the non-breeding season (experiment 1) the concentrations of follistatin in all vessels were about 2-fold higher (P<0·001) than during the breeding season (experiment 2). Concentrations of follistatin were measured in the testicular tissue of the ram, bull, monkey and rat and were found to be 13·6, 2·1, 2·5, 0·8 ng/g testis respectively. In experiment 3, blood samples were collected every 15 min for 4 h from castrated rams (n=6) in the absence of treatment with testosterone propionate (TP) and after 7 days of treatment with a physiological dose of TP during the breeding and non-breeding seasons. There was no effect of stage of breeding season or TP on the plasma concentrations of follistatin and these concentrations in the castrated rams were similar to the concentrations in the intact rams in experiment 2. In experiment 4, the function of Leydig cells was stimulated by administration of human chorionic gonadotrophin but this had no effect on plasma concentrations of follistatin. These experiments show that the concentrations of follistatin in the plasma of rams are measurable, that the testis is not the major contributor to circulating follistatin and that there is no significant uptake or production of follistatin by the head in rams. It appears that the contribution of the testis to circulating follistatin may vary with the stage of the breeding season, being greater during the non-breeding season than the breeding season. The gonadotrophins and testosterone do not appear to have a direct effect on the secretion of follistatin in rams. The increase in concentrations of circulating follistatin during surgery and more frequent blood sampling suggest a stress-related effect on the production of follistatin. Journal of Endocrinology (1996) 149, 55–63

Association of Retained Fetal Membranes with Haematological Profile in Crossbred Cows

2017 ◽  
Vol 12 (3) ◽  
Author(s):  
K. Rokde ◽  
S. Kumar ◽  
A. Bhardwaz ◽  
S. S. Mahour ◽  
S. P. Nema ◽  
...  
Keyword(s):  
Eosinophil Count ◽  
Jugular Vein ◽  
Dairy Farm ◽  
Fetal Membranes ◽  
Monocyte Count ◽  
Blood Samples ◽  
Mean Values ◽  
Crossbred Cows ◽  
Haematological Profile ◽  
The Mean

This study was carried out on clinical cases of retained fetal membranes in crossbred cows presented at College Clinics and College dairy farm and from Villages in and around Mhow. The blood samples were collected from jugular vein just before 12 hr. postpartum and on 7th day postpartum. Haematological profile revealed that the mean values of haemoglobin, neutrophil and monocyte count after 12 hrs and 7th day postpartum were significantly lower and lymphocyte count was significantly higher in RFM cows (n=18) than normally calved cows (n=6). The differences in mean TLC, eosinophil and basophil counts were non-significant at 12 hrs postpartum, however on 7th day postpartum the TLC and eosinophil count were significantly higher and basophil count was non-significantly different in RFM cows than the normally calved cows.

scholarly journals Surrogate Indexes of Insulin Resistance in Dairy Goats: Transitional Variation in Subclinical Hyperketonemia

2021 ◽  
Vol 8 (6) ◽  
pp. 102
Author(s):  
Siqi Liu ◽  
Yezi Kong ◽  
Jing Wen ◽  
Yan Huang ◽  
Yaoquan Liu ◽  
...  
Keyword(s):  
Insulin Resistance ◽  
Transition Period ◽  
Jugular Vein ◽  
Metabolic Parameters ◽  
Model Assessment ◽  
Early Lactation ◽  
Homeostasis Model Assessment ◽  
Dairy Goats ◽  
Blood Samples ◽  
Normal Glucose

Background: Dairy goats are highly susceptible to subclinical hyperketonemia (SCHK) during the transition period. This study aimed to compare the variation in metabolic parameters and surrogate indexes of insulin resistance (sIR) between goats with SCHK and clinically healthy (HEAL) goats during the transition period. Methods: Twenty Guanzhong dairy goats were assorted to HEAL (n = 10) and SCHK (n = 10) groups according to the blood β-hydroxybutyrate (BHBA) concentrations. The blood samples were taken from the jugular vein of each goat at −3, −2, −1, 0 (partum), +1, +2, and +3 weeks relative to kidding to analyses GLU and INS. The sIR was calculated from blood metabolic parameters. Results: Compared with the HEAL goats, the insulin concentrations were significantly higher in SCHK goats during the first three weeks postpartum. The QUICKI, revised QUICKI (RQUICKI), and RQUICKIBHBA were significantly lower in goats with SCHK at 1 week postpartum, while the homeostasis model assessment-IR (HOMA-IR) was significantly higher. Conclusion: Goats with SCHK made more efforts through elevated insulin levels at early lactation than HEAL goats, thereby maintaining the normal glucose concentrations.

scholarly journals Diagnosis of Fibrotic Distal Ileum Stenosis after Ischemic Enteritis Using Transabdominal Ultrasonography

2021 ◽  
pp. 568-577
Author(s):  
Ryo Katsumata ◽  
Noriaki Manabe ◽  
Masaki Matsubara ◽  
Jun Nakamura ◽  
Kazuma Kawahito ◽  
...  
Keyword(s):  
Blood Flow ◽  
Case Reports ◽  
Blood Perfusion ◽  
Distal Ileum ◽  
Wall Thickening ◽  
Current Case ◽  
Abdominal Ultrasonography ◽  
Transabdominal Ultrasonography ◽  
Stenotic Lesion ◽  
Ischemic Enteritis

Ischemic enteritis (IE) is a rare disorder which is caused by inadequate blood flow to small intestine. The diagnostic procedure of this disease has not sufficiently established because of its rarity. Here, we report a case of IE in a hemodialysis-dependent 70-year-old man and summarize the diagnostic options for IE. The patient was admitted to our hospital because of acute abdominal distention and vomiting. He presented with mild tenderness in the lower abdomen and slightly elevated C-reactive protein level as revealed by blood tests. Radiographic imaging showed small bowel obstruction due to a stricture in the distal ileum. Contrast-enhanced abdominal ultrasonography revealed a 7-cm stenotic site with increased intestinal wall thickening, which preserved mucosal blood perfusion. Elastography revealed a highly elastic alteration of the stenotic lesion, indicating benign fibrotic changes resulting from chronic insufficient blood flow. Based on a clinical diagnosis of IE with fibrous stenosis, a partial ileostomy was performed. After surgical treatment, oral intake was initiated without recurrence of intestinal obstruction. Pathological findings revealed deep ulceration with inflammatory cell infiltration at the stenotic site. Occlusion and hyalinization of the venules in the submucosal layer indicated IE. In addition to current case, we reviewed past case reports of IE. Through this case presentation and literature review, we summarize the usefulness and safety of transabdominal ultrasonography for diagnosing IE.

Automatic blood sampling equipment for use in studies of animal physiology

1998 ◽  
Vol 66 (3) ◽  
pp. 769-775 ◽  
Author(s):  
P. J. Goddard ◽  
G. J. Gaskin ◽  
A. J. Macdonald
Keyword(s):  
Jugular Vein ◽  
Blood Sampling ◽  
Start Time ◽  
Confounding Effect ◽  
Blood Samples ◽  
Animal Physiology ◽  
Vein Catheter ◽  
The Subject ◽  
Large Animals ◽  
Jugular Vein Catheter

AbstractA device for the collection of discrete blood samples from large animals has been developed to allow studies of physiology to be undertaken without the confounding effect of restraint. A microprocessor controlled unit (measuring 180 × 110 × 90 mm), weighing less than 1-2 kg, is mounted on the back of the experimental subject using a simple harness. A sampling line is connected to a previously inserted jugular vein catheter. Samples of blood (approx. 5 ml) are collected at pre-determined times following a start time which can be delayed by up to 48 h to allow the subject to recover from any effects of attachment of the sampler. The results from three studies suggest that the device offers a novel way to overcome a number of difficulties which occur when conventional methods are used to collect blood in experiments.

2 EVIDENCE FOR THE PRESENCE OF OVULATION-INDUCING FACTOR IN PORCINE AND EQUINE SEMINAL PLASMA

2009 ◽  
Vol 21 (1) ◽  
pp. 101 ◽  
Author(s):  
O. A. Bogle ◽  
D. Ambati ◽  
R. P. Davis ◽  
G. P. Adams
Keyword(s):  
Seminal Plasma ◽  
Optimal Dose ◽  
Positive Control ◽  
Negative Control ◽  
Response To Treatment ◽  
Preovulatory Follicle ◽  
Progesterone Concentration ◽  
Control Groups ◽  
Plasma Progesterone ◽  
Blood Samples

The presence of an ovulation-inducing factor (OIF) in the seminal plasma of llamas and alpacas (reflex ovulators) and cattle (spontaneous ovulators) has been reported previously (Ratto MH et al. 2006 Theriogenology 66, 1102–1106). The presence of this protein in unrelated species supports the hypothesis that OIF is a conserved factor among species. The objectives of this study were to determine if OIF was present in equine and porcine seminal plasma, and whether the proportion of test animals (llamas) that ovulated in response to treatment with seminal plasma was related to dose. In Experiment 1, female llamas were assigned randomly to four groups (n = 8 or 9 per group) and treated intramuscularly with 1 mL llama seminal plasma (positive control), 3 mL equine seminal plasma, 3 mL porcine seminal plasma, or 2 mL saline (negative control). Ovulation and maximum corpus luteum diameter were compared using ultrasonography and confirmed with blood samples taken on Day 7 (Day 0 = day of treatment) to determine plasma progesterone concentration. The diameter of the preovulatory follicle at the time of treatment did not differ among groups. Equine seminal plasma induced ovulations in 3/8 (38%) llamas compared to 0/8 (0%) llamas treated with saline or porcine seminal plasma (P = 0.1). The proportion of females that ovulated was lower in the equine group (P < 0.01) compared with those animals treated with llama seminal plasma (9/9; 100%). Of the animals that ovulated, maximum CL diameter did not differ between llama and equine seminal plasma-treated groups (mean ± SEM; 11.1 ± 1.1, 11.5 ± 1.5, respectively). Similarly, progesterone concentrations were not different among llamas treated with llama seminal plasma or equine seminal plasma (mean ± SEM; 3.1 ± 0.4, 3.7 ± 1.2, respectively). The design of Experiment 2 was the same, but the dose of equine and porcine seminal plasma was increased to 8 mL and 10 mL, respectively. The proportion of females that ovulated was less (P < 0.05) in equine (2/9) and porcine (3/9) seminal plasma groups compared with the group treated with llama seminal plasma (9/9). There were no ovulations detected in llamas treated with saline (0/8). Although differences between equine, porcine, and negative control groups did not reach significance, results provide some evidence for the presence of OIF in equine and porcine seminal plasma. The effect of dose of equine and porcine seminal plasma is equivocal, suggesting that the concentration of OIF in the seminal plasma of these species may be very low and the optimal dose for inducing ovulation in test animals had not been reached. Research supported by the Natural Sciences and Engineering Council of Canada.

57 DIETARY CLA SUPPLEMENTATION AFFECTS LUTEAL GENE EXPRESSION AND PERIPHERAL BLOOD PROGESTERONE CONCENTRATION IN CATTLE

2012 ◽  
Vol 24 (1) ◽  
pp. 140
Author(s):  
H. Stinshoff ◽  
E. Onnen-Lübben ◽  
S. Wilkening ◽  
A. Hanstedt ◽  
H. Bollwein ◽  
...  
Keyword(s):  
Gene Expression ◽  
Corpus Luteum ◽  
Control Group ◽  
Progesterone Concentration ◽  
Blood Samples ◽  
Oral Supplementation ◽  
Group I ◽  
Pregnancy Status ◽  
Gene Transcripts ◽  
Group Ii

Shortly after parturition the metabolic situation of high-yielding dairy cows is often dominated by a negative energy balance. These effects affect the whole animal and may especially be detected in the reproductive tract, where they result in reduced fertility. An oral supplementation with dietary fats is often used to counteract by reducing milk fat content and, thus, supplying the individual animal with an increased amount of energy. The focus of the present study was to analyse the effects of an oral supplementation with conjugated linoleic acids (CLA) on corpus luteum (CL) function. Healthy Holstein-Friesian cows and heifers were randomly allocated to 2 treatment groups (Group 1: 50 g of CLA day–1 per animal, 2 heifers, 6 cows; Group 2: 100 g of CLA day–1 per animal, 2 heifers, 6 cows) and 1 control group (Ctl; 0 g of CLA day–1 per animal, 3 heifers, 4 cows). Feeding of the supplement began shortly after calving. After calving, all animals were subjected to a standard synchronisation protocol and experienced AI on Day 59 ± 3. Following AI, transvaginal biopsies of the corpus luteum were obtained of pregnant (Group I: n = 4; Group II: n = 4; Ctl: n = 4) and nonpregnant (Group I: n = 4; Group II: n = 4; Ctl: n = 3) animals on Days 6, 13 and 20 post-AI. Animals deemed pregnant on Day 28 were again biopsied on Day 42. Additionally, blood samples were taken from the vena sacralis mediana at the time of each biopsy. The biopsies were analysed regarding the relative abundance of 8 gene transcripts (VEGF, ECE1, PLA2G4A, PTGS2, PTGFR, PPARG, STAR and HSD3B1) via RT-qPCR. Blood samples were analysed for their concentration of progesterone through a radioimmunoassay (RIA). Statistical analysis for both datasets was performed via a 3-way ANOVA with adjoining Tukey test. The expression of 7 of these genes was affected by 1, 2, or all 3 of the following factors: day of cycle (VEGF, ECE1, PLA2G4A, PTGFR, STAR and HSD3B1), pregnancy status (ECE1, PTGFR and HSD3B1) and CLA supplementation (ECE1, PTGS2, PTGFR, STAR and HSD3B1). The effects of the CLA supplementation could be seen as a down-regulation in the mentioned gene transcripts. Progesterone concentrations differed significantly in dependency of the pregnancy status (significantly higher in pregnant vs nonpregnant individuals) of the animals, as well as during the days of the oestrous cycle (physiological progesterone curve with highest values on Day 13 of these samples). An effect of the oral supplementation with CLA could be detected during the early luteal phase (Day 6) where animals that had received 100 g of CLA day–1 had a significantly lower blood progesterone concentration than those receiving 50 g of CLA day–1 or no CLA. In conclusion, dietary CLA supplementation has an effect on luteal gene expression and functionality. The authors thank the DFG (German Research Foundation) for their financial support (PAK286/1; WR154/1-1).

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