scholarly journals Phytochemistry and antioxidant activity of Amaranthus viridis L (Green leaf)

2021 ◽  
Vol 12 (2) ◽  
pp. 306-314
Author(s):  
Ezekwe Ahamefula Sunday ◽  
Wokocha Peter Gift ◽  
Woha James Boobondah
Keyword(s):  
Antioxidant Activity ◽  
Erucic Acid ◽  
Leaf Extract ◽  
Green Leaf ◽  
Analytic Method ◽  
Hexadecanoic Acid ◽  
Leaf Sample ◽  
Standard Methods ◽  
Food Industries ◽  
Body Care

Phytochemistry and antioxidant activity of Amaranthus viridis L (Green leaf) were undertaken with standard methods. The leaf sample after being screening for phytochemicals, contained tannins, saponins, flavonoids, alkaloids, steroids, phenolic compounds, proteins and anthraquinones at different concentrations. A further study using Gas Chromatography-Mass Spectrophotometry (GC-MS) analytic method on the leaf sample revealed a total of twenty-two detailed compounds among which are 3-Hydroxy-N-methylphenethylamine, Erucic acid, n-Hexadecanoic acid, 1,2-Propanediol,3-chloro-, and Cystamine. 3-Hydroxy-N-methylphenethy lamine had the highest retention time; Erucic acid had the highest molecular weight while n-Hexadecanoic acid had the highest peal area. Most of the compounds have important applications in body care products and cosmetics, pharmaceutical or food industries. A. viridis leaf extract exhibited a better antioxidant activity against the ascorbic acid used as control in the present study. The phytochemicals present in the leaf sample could be behind its antioxidant activity. The study has shown the phytochemistry and antioxidant activity of A. viridis leaf sample.

scholarly journals Phytochemical Screening, GC-MS Analysis and Antioxidant Activity of CurcurbitapepoL. usingIts Leaf Sample

2021 ◽  
pp. 33-45
Author(s):  
Ezekwe Ahamefula Sunday ◽  
Nwadike Constance Nnedimma ◽  
Wokocha Gift Peter ◽  
George Boma Orlando
Keyword(s):  
Mass Spectrometry ◽  
Molecular Weight ◽  
Antioxidant Activity ◽  
Gas Chromatography Mass Spectrometry ◽  
State University ◽  
Phytochemical Screening ◽  
Leaf Sample ◽  
Standard Methods ◽  
Ms Analysis ◽  
Ethylhexyl Phthalate

This study evaluated the phytochemical screening, gas chromatography-mass spectrometry (GC-MS) analysis and antioxidant activity of Curcurbitapepo L. using its leaf sample with standard methods. The sample used for the study was procured from Imo State University school farm and was properly identified. Result of phytochemical screening revealed the presence of  saponins, flavonoids, alkaloids, steroids, phlobactannins, proteins, and anthraquinnones, while the GC-MS analysis revealed a total of 78 compounds, out which Bis(2-ethylhexyl) phthalate (C24H38O4) had the highest molecular weight, 2,4,6-Octatriene, 2,6-dimethyl- (C10H16) had the highest peak area of 10.21% while Morphinan-6-ol, 4,5-epoxy-N-methyl-, (5α 6α- (C17H21NO2) had the highest retention time. The antioxidant activity of the studied sample was enhanced against the control. Some of the compounds as revealed by GC-MS analysis could be of healthcare or industrial importance.  There is need for further studies on the leaf sample to ascertain further the observations of the present study. This study has evaluated the phytochemical screening, GC-MS analysis and antioxidant activity of C.pepo L. using its leaf sample.

scholarly journals Characterization of Phytoconstituents from Alcoholic Extracts of Four Woody Species and Their Potential Uses for Management of Six Fusarium oxysporum Isolates Identified from Some Plant Hosts

Plants ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1325
Author(s):  
Mohamed Z. M. Salem ◽  
Abeer A. Mohamed ◽  
Hayssam M. Ali ◽  
Dunia A. Al Al Farraj
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Benzoic Acid ◽  
Fusarium Oxysporum ◽  
Leaf Extract ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Bark Extract ◽  
Leaf Extracts ◽  
Conium Maculatum

Background: Trees are good sources of bioactive compounds as antifungal and antioxidant activities. Methods: Management of six molecularly identified Fusarium oxysporum isolates (F. oxy 1, F. oxy 2, F. oxy 3, F. oxy 4, F. oxy 5 and F. oxy 6, under the accession numbers MW854648, MW854649, MW854650, MW854651, and MW854652, respectively) was assayed using four extracts from Conium maculatum leaves, Acacia saligna bark, Schinus terebinthifolius wood and Ficus eriobotryoides leaves. All the extracts were analyzed using HPLC-VWD for phenolic and flavonoid compounds and the antioxidant activity was evaluated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging and β-carotene-linoleic acid (BCB) bleaching assays. Results: In mg/kg extract, the highest amounts of polyphenolic compounds p-hydroxy benzoic, benzoic, gallic, and rosmarinic acids, with 444.37, 342.16, 311.32 and 117.87, respectively, were observed in C. maculatum leaf extract; gallic and benzoic acids with 2551.02, 1580.32, respectively, in A. saligna bark extract; quinol, naringenin, rutin, catechol, and benzoic acid with 2530.22, 1224.904, 798.29, 732.28, and 697.73, respectively, in S. terebinthifolius wood extract; and rutin, o-coumaric acid, p-hydroxy benzoic acid, resveratrol, and rosmarinic acid with 9168.03, 2016.93, 1009.20, 1156.99, and 574.907, respectively, in F. eriobotryoides leaf extract. At the extract concentration of 1250 mg/L, the antifungal activity against the growth of F. oxysporum strains showed that A. saligna bark followed by C. maculatum leaf extracts had the highest inhibition percentage of fungal growth (IPFG%) against F. oxy 1 with 80% and 79.5%, F. oxy 2 with 86.44% and 78.9%, F. oxy 3 with 86.4% and 84.2%, F. oxy 4 with 84.2, and 82.1%, F. oxy 5 with 88.4% and 86.9%, and F. oxy 6 with 88.9, and 87.1%, respectively. For the antioxidant activity, ethanolic extract from C. maculatum leaves showed the lowest concentration that inhibited 50% of DPPH free radical (3.4 μg/mL). Additionally, the same extract observed the lowest concentration (4.5 μg/mL) that inhibited BCB bleaching. Conclusions: Extracts from A. saligna bark and C. maculatum leaves are considered potential candidates against the growth of F. oxysporum isolates—a wilt pathogen—and C. maculatum leaf as a potent antioxidant agent.

scholarly journals Supercritical Impregnation of PLA Filaments with Mango Leaf Extract to Manufacture Functionalized Biomedical Devices by 3D Printing

Polymers ◽  
2021 ◽  
Vol 13 (13) ◽  
pp. 2125
Author(s):  
José María Rosales ◽  
Cristina Cejudo ◽  
Lidia Verano ◽  
Lourdes Casas ◽  
Casimiro Mantell ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
3D Printing ◽  
Polylactic Acid ◽  
Leaf Extract ◽  
Three Dimensional ◽  
3D Printer ◽  
Pharmacological Properties ◽  
Bioactive Properties ◽  
Supercritical Impregnation ◽  
Mango Leaves

Polylactic Acid (PLA) filaments impregnated with ethanolic mango leaves extract (MLE) with pharmacological properties were obtained by supercritical impregnation. The effects of pressure, temperature and amount of extract on the response variables, i.e., swelling, extract loading and bioactivity of the PLA filaments, were determined. The analysis of the filaments biocapacities revealed that impregnated PLA filaments showed 11.07% antidenaturant capacity and 88.13% antioxidant activity, which after a 9-day incubation shifted to 30.10% and 9.90%, respectively. Subsequently, the same tests were conducted on printed samples. Before their incubation, the printed samples showed 79.09% antioxidant activity and no antidenaturant capacity was detected. However, after their incubation, the antioxidant activity went down to only 2.50%, while the antidenaturant capacity raised up to 23.50%. The persistence of the bioactive properties after printing opens the possibility of using the functionalized PLA filaments as the feed for a three-dimensional (3D) printer.

scholarly journals Antioxidant activity, xanthine oxidase inhibitory activity, and compounds determination of Cipadessa baccifera leaf extract

2021 ◽  
Author(s):  
Syarifah Ramadhani Lubis ◽  
Subandi Subandi ◽  
Muntholib Muntholib ◽  
Jamilah Abbas ◽  
Tjandrawati Mozef
Keyword(s):  
Antioxidant Activity ◽  
Xanthine Oxidase ◽  
Inhibitory Activity ◽  
Leaf Extract

scholarly journals In-vitro antimicrobial and antioxidant activity of Argyreia cuneata (Willd.) Ker Gawl. (Convolvulaceae)

2018 ◽  
Vol 8 (6) ◽  
pp. 22-27
Author(s):  
TR Prashith Kekuda ◽  
Nitish A. Bharadwaj ◽  
MB Sachin ◽  
BK Sahana ◽  
GS Priyanka
Keyword(s):  
Antioxidant Activity ◽  
Antifungal Activity ◽  
Leaf Extract ◽  
Shigella Flexneri ◽  
Radical Scavenging ◽  
Flower Extract ◽  
Stem Extract ◽  
Poisoned Food Technique ◽  
Antimicrobial And Antioxidant Activity

Objectives: Argyreia cuneata (Willd.) Ker Gawl. belongs to the family Convolvulaceae. The present study was performed to screen the potential of crude extract of various parts of A. cuneata to exhibit antimicrobial activity. Methods: Extraction of shade dried and powdered leaf, stem and flower of A. cuneata was carried out by maceration technique. Antibacterial and antifungal activity of extracts was evaluated by Agar well diffusion and Poisoned food technique respectively. Antioxidant activity was determined by DPPH radical scavenging, ABTS radical scavenging and ferric reducing assays. Results:  All extracts were effective in inhibiting test bacteria and the susceptibility of bacteria to extracts was in the order: Bacillus cereus > Shigella flexneri > Escherichia coli > Salmonella typhimurium. Leaf extract and stem extract exhibited highest and least antibacterial activity, respectively. Extracts were effective in causing inhibition of seed-borne fungi viz. Aspergillus niger and Bipolaris sp to >50%. Leaf extract exhibited marked antifungal activity followed by flower extract and stem extract. All extracts were shown to exhibit concentration dependent scavenging and reducing activity. Antioxidant activity of extracts observed was in the order: leaf extract > flower extract > stem extract.  Conclusion: Among various parts of A. cuneata, leaf extract exhibited marked antimicrobial and antioxidant activity. The plant can be employed as an effective antimicrobial and antioxidant agent in suitable form. Further studies may be undertaken to recover phytochemicals from the plant and to investigate the antimicrobial and antioxidant activity of isolated components. Keywords: Argyreia cuneata, Maceration, Antimicrobial, Agar well diffusion, Poisoned food technique, Antioxidant

scholarly journals Evaluation of Antioxidant Potential of Stem and Leaf Extracts of Himalayan Tinospora cordifolia Hook.f. & Thomson

2021 ◽  
Vol 9 (1) ◽  
pp. 2-8
Author(s):  
Geetanjali Upadhyay ◽  
Lalit M. Tewari ◽  
Geeta Tewari ◽  
Neha Chopra ◽  
Naveen C. Pandey ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Leaf Extract ◽  
Radical Scavenging ◽  
Aluminium Chloride ◽  
Tinospora Cordifolia ◽  
Total Phenolic ◽  
Flavonoid Content ◽  
Leaf Extracts ◽  
The Family ◽  
Stem Extract

Background: Medicinal plants are considered a rich source of ingredients, which can be used in drug development and synthesis. Tinospora cordifolia (Wild.) Hook.f. & Thomson, commonly known as guduchi, heart-leaved moonseed and giloya is a herbaceous vine of the family Menispermaceae, has several beneficial properties including antioxidant activity. Aim: The present study was carried out to analyze the antioxidant activity of leaf and stem extracts of Tinospora cordifolia by using DPPH (1,1-Diphenyl-2-picrylhydrazyl) and ABTS (2,2´-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid)) free radical scavenging assays. Materials and Methods: Dried and powdered leaves and stem of T. cordifolia were extracted with methanol. Ascorbic acid was taken as standard. Total phenolic content was estimated by using Folin-ciocalteu's reagent while total flavonoid content by aluminium chloride reagent to find the correlation of polyphenols with antioxidant activity. ABTS assay of methanolic leaf and stem extracts showed the highest scavenging activity as compared to the DPPH assay. Results: Methanolic stem extract showed higher phenolic and flavonoid content along with antioxidant activity as compared to the methanolic leaf extract. Conclusion: The stem extract exhibited more antioxidant activity than the leaf extract with regards to the all parameters analyzed.

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