scholarly journals PENGARUH PENAMBAHAN SERBUK DAUN TANAMAN KAYU MANIS (Cinnamomum burmannii) PADA PAKAN TERHADAP PROFIL DARAH (KADAR HEMATOKRIT, KADAR HEMOGLOBIN, TOTAL LEUKOSIT DAN TOTAL ERITROSIT) IKAN NILA (Oreochromis niloticus) YANG DIINFEKSI Streptococcus agalactiae

2018 ◽  
Vol 1 (1) ◽  
pp. 24
Author(s):  
Sri Lestari ◽  
Firma Fika Rahmawati ◽  
Rahmad Jumadi

Bacterial disease like Streptococcosis which is caused by Streptoccoccusagalactiae becomesthe most important problem that often arise in the aquaculture business. The disease can be100% cause of death. One of the prevention efforts is using phytopharmaca in cinnamon leaf(Cinnamomunburmanni). This traditional herb is eco-friendly, safe for consumers and it does nothave any residue. Chemical compounds contained in cinnamon leaves have function asantioxidants, to increase metabolism, growth and non-specific immune response in fish. Thisstudy is to determine the effect and find the best dose of the addition of cinnamon leaf powder infood given to Tilapia that infected by Streptococcus agalactaiae. The tested dose is 0%, 0.25%,0.5%, 1%. Observed parameters were blood profile (hematocrit level, hemoglobin level, totalerythrocytes and total leukocytes) and water quality. The results were analyzed by ANOVA usingRAL with 95% credibility level. The results obtained in the study by adding cinnamon leafpowder to the given food to tilapia that was infected by Streptococcus agalactiae affect the bloodprofile (hematocrit level, hemoglobin level, total erythrocytes and total leukocytes). A dose of0.25% is the best dose for hematocrit level, hemogobline level and erythrocytes total. While thedose of 1% is the best dose for the total value of leukocytes. Doses of 0.25% and 1% can beapplied to tilapia culture to prevent Streptococcosis disease caused by Streptococcus agalactiaebacteria.

2018 ◽  
Vol 1 (1) ◽  
pp. 40
Author(s):  
Sri Wulandari ◽  
Rahmad Jumadi ◽  
Firma Fika Rahmawati

The main problem in the cultivation of tilapia is the attack of bacterial disease Streptococcosiscaused by Streptococcus agalactiae bacteria. Alternative measures to prevent the disease ofStreptocococis can be through the use of phytopharmaca materials. One of the ingredients ofphytopharmaca can be used is cinnamon plant. Cinnamon leaves contain several types of activesubstances such as tannins, eugenol, safrole, calcium oxalate, resin, saponins, tanners, andsinamaldehid. The objective of the study of effectiveness of leaf powder of cinnamon plant toleukocyte differential and phagocytic activity in tilapia (Oreochromis niloticus) infected byStreptococcus agalactiae is to know the influence and dosage of cinnamon leaf powder in feedtoLeukocyte Differentiation and Phagocytosis Activity Postcainfection Streptococcus agalactiaein tilapia (Oreochromis niloticus). This research used 3treatment 4 replication and control withdose K- = challenge test, K+ = without test challenge, A = dose 0,25%, B = dose 0,5%, C = 1%.The parameters observed are Leukocyte Differential and Phagocytosis Activity. Dosage 0.5%addition of leaf powder cinnamon plant on feed is the best dose. Giving of cinnamon leaf powderin feed influenced to increase of leukocyte difference especially on monocyte cell and neutrophilcell was significantly different (P>0,05) than K+ without addition of cinnamon leaf powderwhile phagocytic activity had an effect on Streptococcus agacatiae.


2019 ◽  
Vol 4 (2) ◽  
pp. 22-28
Author(s):  
Wahyu Dwi Putranto ◽  
Denny Syaputra ◽  
Eva Prasetiyono

Tilapia (Oreochromis niloticus) is populer as resistant fish to environmental changes. Cultivating fish with a high stocking density can cause a decrease in water quality. It has an impact on susceptibility to disease due to decreased water quality. Immunostimulants from Phyto-pharmacy are needed to improve the non-specific immune system of fish from natural ingredients. Bay leaves (Syzigium polyanthum) have the potential to be used as an immunostimulant in fish. This study aimed to evaluate the extract of Salam leaves fortified in feed against the blood profile of Tilapia. The research was carried out in April - May 2018 using a completely randomized design (CRD) with 4 treatments and 3 replications. Salam leaf extract through feed affects the increase of leucocytes and blood erythrocytes of tilapia but does not affect hemoglobin levels and hematocrit levels. Doses of 2x10-2 g mL-1 increased fish blood leukocytes on day 21st to be the best treatment in this study.


2021 ◽  
Vol 7 (2) ◽  
pp. 153-163
Author(s):  
Zubyda Mushtari Nadia ◽  
Prosun Roy ◽  
Sayed Mashequl Bari ◽  
Md Abdus Salam

Management of fish health is one of the main considerations in aquaculture and different plant compounds are being used for supporting fish health to minimize the negative impacts of synthetic aqua drugs. In the present experiment, potentiality of moringa (Moringa oleifera; Lamarck, 1785) leaf as a nutritious dietary source for tilapia (Oreochromis niloticus; Linnaeus, 1758) fish was tested and the duration was two months from 30th September to 30th November, 2020. The moringa leaves were brought, cleaned, dried, and finally crushed into powder. Three experimental diets were formulated using the processed moringa leaf powder (MLP) at the rate of 0% (MLP0%) as control, 10% (MLP10%) and 20% (MLP20%) as treatment mixing with rice bran, wheat bran, mustard oil cake, fish meal, soya oil and vitamin-mineral premix. Fifteen tilapia fingerlings having average initial length 10.88±0.11 cm and initial weight 29.06±0.50 g was stocked in each tank with 90 L water. Sixty days feeding trial was performed with three replications of each treatment. The fishes were fed with formulated feeds twice daily at 9 am and 4 pm at a rate of 3% of their body mass. Sampling of fish and water quality parameters were carried out at twelve days interval. Moreover, the blood glucose and cholesterol of tilapia were measured monthly. Final length, final weight, weight gain, percent weight gain, feed conversion ratio (FCR), specific growth rate (SGR) and production of tilapia were significantly different among the treatments. The highest FCR (3.17±0.25) and SGR (1.33±0.12 %) values were in MLP20% and MLP10%, respectively. In the experiment, the highest and the lowest tilapia production were 9.21±0.39 and 7.39±0.35 kg m-3 in MLP10% and MLP20%, respectively. The blood glucose values were significantly different among the treatments (p< 0.05) and the highest value was in MLP0% (48.00±2.00 mg dl-1). Moreover, the highest and the lowest blood cholesterol was found in MLP0% (177.67±2.52 mg dl-1) and MLP20% (148.33±1.53 mg dl-1), respectively whereas the values were highly significantly different among the treatments (p≤ 0.01). Water quality parameters were statistically similar among the treatments (p> 0.05) and the values were within acceptable range for tilapia culture. Asian J. Med. Biol. Res. 2021, 7 (2), 153-163


Author(s):  
Arne A Ratulangi ◽  
Reiny Tumbol ◽  
Hengky Manoppo ◽  
Henneke Pangkey

This study aims to apply vaccination against bacterial disease. The purpose of vaccination is to trigger the immune respone both non-specific and specific of fish against bacteria Motile Aeromonas Septicaemia (MAS) caused by Aeromonas hydrophila. The vaccination for fish with different ages: 2-3 weeks and 5 weeks were done using immersion method. The fish were re-vaccinated (booster) after two weeks of the first vaccination. The survival rate was < 50 % for juveniles 2-3 weeks and > 50% for juveniles 5 weeks. Survival rate for juveniles 5 weeks was higher than juveniles of 2-3 weeks. This shows that organs of juveniles of 5 weeks were more complete than the 2-3 weeks juveniles. The age of fish is one of the important factors for successfully vaccination. Penelitian ini bertujuan untuk melakukan penerapan vaksinasi terhadap penyakit bakterial. Vaksinasi ditujukan untuk merangsang respon kekebalan non- spesifik dan spesifik pada tubuh ikan terhadap penyakit Motile Aeromonas Septicaemia (MAS) yang disebabkan oleh bakteri Aeromonas hydrophila. Vaksin diberikan pada benih ikan nila yang berbeda umur yaitu 2-3 minggu dan 5 minggu dengan menggunakan metode perendaman. Ikan divaksinasi ulang (booster) setelah 2 (dua) minggu dari vaksinasi yang pertama. Prosentase kelangsungan hidup < 50 % untuk benih umur 2-3 minggu dan > 50% untuk benih umur 5 minggu. Jumlah kematian benih umur 2-3 minggu lebih tinggi dari 5 minggu. Hal ini menunjukkan bahwa fungsi organ benih umur 5 minggu telah lebih lengkap dari pada benih umur 2-3 minggu. Umur ikan merupakan salah satu faktor penting penentu keberhasilan suatu kegiatan vaksinasi.


2019 ◽  
Vol 15 ◽  
pp. 100232 ◽  
Author(s):  
Mengmeng Yi ◽  
Miao Wang ◽  
Zhonghui Li ◽  
Zhigang Liu ◽  
Changbin Song ◽  
...  

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