scholarly journals Features of hemostasis before and long-term periods in patients after ovarian apoplexy

2021 ◽  
Vol 3 ◽  
pp. 71-74
Author(s):  
O.M. Ishak
Keyword(s):  
Abdominal Cavity ◽  
Fibrinolytic System ◽  
Coagulation System ◽  
Laboratory Research ◽  
Control Group ◽  
Thrombin Time ◽  
Pathological Changes ◽  
Acute Period ◽  
Hemostasis System

The objective: to reveal the features of the parameters of the blood coagulation system in women in dynamics – from the acute period of ovarian apoplexy to the long-term postoperative period.Materials and methods. The study involved 82 women (main group, n=82), operated on for ovarian apoplexy, and 30 patients in the control group (n=30), who had no history of this pathology. All patients underwent a comprehensive examination using clinical, instrumental and laboratory research methods. The groups were homogeneous in age (average age was 26,1±2,3 years) and statistically comparable.Results. During the study, it was found that in the acute period of ovarian apoplexy, pathological changes were found in the plasma, vascular-platelet components and the fibrinolytic system of hemostasis, as evidenced by the lengthening of the thrombin time to 22,3±1,32 seconds, an increase in the concentration of fibrinogen to 6,73±0,64 g/l, SFMC up to 0,7±0,03 g/l and platelet hyperaggregation, stimulated by ADF, ristocetin and collagen, and reaching 80%. The extremely high content of D-dimer in the abdominal cavity in patients with ovarian apoplexy indicated increased thrombus formation in the damaged ovary, followed by activation of the fibrinolytic system and lysis of fibrin clots. Genetic thrombogenicity was found in general in 80,4% of patients, including those with a predominant lesion of the folate cycle enzymes - in every second patient.Positive dynamics with a tendency to normocoagulation was observed in the distant postoperative period. In the reference range were SFMC indices in 86,6% of cases, fibrinogen and thrombin time in 89,1% of cases, ristocetin-stimulated aggregatogram in 95,1% of cases (p<0,05).Conclusions. In women who underwent ovarian apoplexy, in the acute period of the disease, pathological changes in the vascular-platelet link of the hemostasis system were observed. The diagnosed thrombophilic disorders could lead to a deterioration in the rheological properties of blood, a violation of the processes of oxygenation and tissue trophism. The dynamic study of the hemostasis system in the acute and late postoperative periods of patients with ovarian apoplexy, as well as the data obtained on the prevalence of genetic thrombogenicity in the examined patients, should be taken into account when managing women from this risk group.

scholarly journals Evaluation Procoagulant Activity and Mechanism of Astragalin

Molecules ◽  
2020 ◽  
Vol 25 (1) ◽  
pp. 177 ◽  
Author(s):  
Changqin Li ◽  
Miyun Hu ◽  
Shengjun Jiang ◽  
Zhenhua Liang ◽  
Jinmei Wang ◽  
...  
Keyword(s):  
Plasma Viscosity ◽  
Procoagulant Activity ◽  
Coagulation System ◽  
Control Group ◽  
Thrombin Time ◽  
Coagulation Time ◽  
Erythrocyte Sedimentation ◽  
Procoagulant Effect

Astragalin, isolated from flowers of Rosa chinensis Jacq., is a kind of flavonoid, with anti-inflammatory, antioxidant, antiviral, analgesic, antibacterial, antiallergic, and antihepatotoxic effects. However, no studieson the procoagulant effect of astragalin have been reported. This study aimed to investigate the procoagulant activity of astragalin and its mechanism. Its procoagulant effect was investigated by activated partial thromboplastin time (APTT), thrombin time (TT), prothrombin time (PT), and fibrinogen (FIB) in vitro, and a rat model established by heparin sodium was used to evaluate the mechanism for the procoagulant effect in vivo. The results showed that astragalin had good procoagulant effects compared with the control group in vitro. Compared with the model group in vivo, astragalin could shorten the coagulation time and significantly increase the number of platelets. Meanwhile, astragalin could significantly reduce the effectual time of PT and APTT and increase the content of FIB. The contents of 6-keto-PGF1α and eNOS significantly decreased. Astragalin could increase whole blood viscosity (WBV), plasma viscosity (PV), erythrocyte sedimentation rate (ESR) and packedcell volume (PCV). All of the above revealed that astragalin had good procoagulant effects by promoting the intrinsic and extrinsic coagulation system.

scholarly journals The influence of hyperprolactinemia on coagulation parameters in females with prolactinomas

2014 ◽  
Vol 142 (5-6) ◽  
pp. 314-319 ◽  
Author(s):  
Milica Medic-Stojanoska ◽  
Gorana Mitic ◽  
Igor Mitic ◽  
Dragan Spasic ◽  
Nikola Curic ◽  
...  
Keyword(s):  
Young Female ◽  
Coagulation System ◽  
Control Group ◽  
Thrombin Time ◽  
Contributing Factors ◽  
Normal Range ◽  
Healthy Female ◽  
Before And After ◽  
Von Willebrand ◽  
D Dimer

Introduction. Currently there is little information on the effects of prolactin (PRL) on the coagulation and fibrinolytic systems. Objective. The aim of this study was to evaluate the effects of hypeprolactinemia on the parameters of the hemostatic system and activation of the coagulation system. Methods. We studied PRL levels, body mass index (BMI), values of activated partial thromboplastin time (aPTT), prothrombin time (PT), thrombin time (TT), D-dimer level, von Willebrand factor antigen (vWFAg) and fibrinogen in 15 young female patients with microprolactinomas before and after therapy and in 15 healthy female controls. Results. As expected, pretreatment PRL levels were significantly higher in patients than in controls (140.90?42.87 vs. 12.53?4.05 ng/ml; p<0.001). PT, although still in the normal range, was prolonged in patients with hyperprolactinemia as compared to the control group (13.53?1.39 vs. 12.65?0.53 s; p=0.03) and normalized after therapy (12.69?0.65 vs. 12.65?0.53 s; p=0.88). TT, although in normal range, was significantly shorter in the hypeprolactinemic patients than in the controls (14.34?4.52 vs. 17.21?1.35 s; p<0.025) and after treatment remained significantly shorter than in the controls (15.17?1.55 vs. 17.21?1.35 s; p<0.0001). D-dimer values before treatment in the patients with hyperproplactinemia were above the normal range (239.47?107.93 vs. 131.27?50.64 ng/ml, p=0.002) and decreased to normal values after therapy (239.47?107.93 vs. 146.60?39.15 ng/ml; p<0.001). D-dimer levels correlated with PRL (r=0.30) and the change in serum D-dimer values significantly correlated with the change in PRL levels during therapy (r=0.62). aPTT, vWFAg and fibrinogen were similar in patients and controls. Conclusion. In our study, increased thrombin generation that resulted in elevated D-dimer levels may be one of the contributing factors to the prethrombotic state in patients with hyperprolactinemia.

Comparative analysis of the results of non-activated thromboelastometry and thromboelastography of venous blood in pregnant women in health and disease

2021 ◽  
Author(s):  
Л.А. Пестряева ◽  
С.В. Кинжалова ◽  
Н.В. Путилова ◽  
С.В. Борисова
Keyword(s):  
Comparative Analysis ◽  
Pregnant Women ◽  
Blood Coagulation ◽  
Main Group ◽  
Coagulation System ◽  
Control Group ◽  
Blood Coagulation Disorders ◽  
Fibrinogen Concentration ◽  
Reference Ranges ◽  
Thrombin Time

Цель исследования: сравнительный анализ неактивированной тромбоэластографии (ТЭГ) и тромбоэластометрии (ТЭМ) в диагностике нарушений свертывающей системы крови при беременности. Материалы и методы. Обследованы 2 группы женщин в III триместре беременности: 44 женщины с привычным невынашиванием беременности в анамнезе (основная группа) и 35 условно здоровых беременных (контрольная группа). Пациентки основной группы получали профилактику тромботических осложнений низкомолекулярными гепаринами (НМГ). Выполнено стандартное исследование свертывающей системы крови: подсчет тромбоцитов, определение концентрации фибриногена, протромбинового времени (ПТ) по Квику, активированного частичного тромбопластинового времени (АЧТВ), тромбинового времени (ТВ), неактивированная ТЭГ и ТЭМ. Результаты. Значимых различий в значениях фибриногена, АЧТВ, ПТ между группами выявлено не было. В обеих группах при ТЭГ/ТЭМ исследовании установлено укорочение интервалов времени свертывания и времени образования сгустка, увеличение плотности сгустка, что соответствует протромботическим изменениям системы гемостаза при беременности. Определены значения показателей неактивированной ТЭГ/ТЭМ в III триместре физиологически протекающей беременности. Заключение. Методы неактивированной ТЭГ и ТЭМ имеют высокую информативность в диагностике нарушений системы гемостаза и могут быть использованы для контроля состояния свертывающей системы крови при беременности наряду с активированными стандартизованными тестами. Objectives: to conduct a comparative analysis of non-activated thromboelastography (TEG) and thromboelastometry (TEM) in the diagnosis of blood coagulation disorders during pregnancy. Patients/Methods. We examined 2 groups of women in III trimester of pregnancy: 44 women with a history of habitual miscarriage (the main group) and 35 relatively healthy pregnant women (the control group). Patients of the main group received prophylaxis of thrombotic complications with low molecular weight heparins (LMWH). A standard study of the blood coagulation system with platelet count, determination of fibrinogen concentration, prothrombin time (PT) by Quick, activated partial thromboplastin time (APTT), thrombin time (TT), non-activated thromboelastography (TЕG) and thromboelastometry (TEM) was performed. Results. There were no signifi cant differences in fi brinogen level, APTT, PT between the groups. In both groups, TEG/TEM study found shortening of the clotting time intervals and clot formation time, an increase in clot density, which corresponds to hemostasis prothrombotic changes during pregnancy. Reference ranges of nonactivated TEG/TEM parameters in III trimester of physiological pregnancy were determined. Conclusions. Methods of non-activated TEG/TEM are highly informative in the diagnosis of hemostasis disorders and can be used, together with activated standardized tests, to monitor blood coagulation during pregnancy.

scholarly journals VEGETATIVE REACTIVITY OF CHILDREN WITH STREPTOCOCCAL TONSILITIS ON THE BACKGROUND OF HERPES VIRAL INFECTION

2021 ◽  
Vol 3 (17) ◽  
pp. 28-35
Author(s):  
V.M. Olkhovska
Keyword(s):  
Heart Rate ◽  
Herpes Virus ◽  
Complete Recovery ◽  
Control Group ◽  
Healthy Children ◽  
Autonomic Reactivity ◽  
Acute Period ◽  
Herpès Virus ◽  
Streptococcal Tonsillitis

Streptococcal infection in children, its complications and adverse long-term consequences remain an urgent problem in pediatrics. Scientists continue to search for factors that shape the course of the disease. One of them is the timely and adequate response of the autonomic nervous system, which provides both short-term and long-term strategic adaptation of the organism. Violations of this adaptation can lead to the presence of comorbid conditions, which are increasingly common. The share of children is growing. infected with the herpes virus group, including human herpes virus type 6 (HHV-6).   The aim of the work was to study the state of vegetative reactivity (VR) of children with streptococcal tonsillitis on the background of HHV-6 infection. In 68 children aged 4 - 15 years with tonsillitis of streptococcal etiology of moderate severity, vegetative reactivity was determined by cardiointervalography (CIG) followed by analysis of heart rate variability (CP) depending on the presence or absence of concomitant HHV-6 type of infection. The studies were performed in the acute period of the disease and in the period of early convalescence. The control group consisted of 20 healthy children, similar in age and sex. Static processing of the obtained results was performed using Excel and Statistica 6.0 using parametric and non-parametric research methods.   Results. In the acute period of streptococcal tonsillitis in children of both groups there was a decrease in the humoral component in the regulation of the system and the level of activity of the parasympathetic ANS, as evidenced by a decrease in Mo and ΔX, independent of background, p> 0.05. There was an activation of the influence of the sympathetic link of the VNS, increased the degree of centralization of heart rate control in all patients, and this was confirmed by an increase in AMO and IN. Prior to the convalescence period, CIG parameters were restored only in patients without concomitant VGL-6 infection, and the interrelationships of the sympathetic and parasympathetic links of the VNS were restructured with increasing role of the latter. In patients with concomitant VGL-6 infection, the values ​​of ΔX and IN differed significantly from the control group, p˂0.05. Thus, in children with streptococcal tonsillitis on the background of HHV-6 infection, there was a decrease in the level of functioning of the sympathetic VNS, but complete recovery did not occur, indicating instability of adaptation processes, depletion of the parasympathetic VNS, which hides the possibility of failure of adaptation, recurrence of the disease and / or chronicity, the development of secondary disorders of the cardiovascular system. Conclusions. The acute period of streptococcal tonsillitis is accompanied by stress of the sympathetic VNS, the severity of which does not depend on the presence of concomitant herpes virus infection. In the period of convalescence of streptococcal tonsillitis in the presence of children with HHV-6 infection there is no complete recovery of autonomic reactivity, among which the most informative are ΔX and IN.

Experimental study on disorders in the activity of macrophages in the acute period of different severities of cerebral stroke

2021 ◽  
Vol 29 (2) ◽  
pp. 201-212
Author(s):  
Andrey E. Kulchikov ◽  
Sergey G. Morozov ◽  
Rashit S. Musin ◽  
Elena A. Grinenko
Keyword(s):  
Experimental Study ◽  
Metabolic Activity ◽  
Functional Activity ◽  
Autologous Blood ◽  
Abdominal Cavity ◽  
Peritoneal Macrophages ◽  
Control Group ◽  
Cerebral Stroke ◽  
Pia Mater ◽  
Acute Period

BACKGROUND: The development of immunosuppression in the acute phase of cerebral stroke may lead to infectious and inflammatory complications. However, data on the functional activity of macrophages are insufficient to understand the pathogenesis of stroke. AIM: This study aimed to investigate the functional activity of macrophages in the acute period of experimental cerebral stroke (ECS) of different severities. MATERIALS AND METHODS: In an experimental study on 45 Wistar rats, the functional and spontaneous metabolic activity of macrophages in the spleen and abdominal cavity and the induced metabolic activity of peritoneal macrophages were evaluated through the reduction of nitro blue tetrazolium via spectrophotometry. The spontaneous and induced adhesive activity and the inhibition of the modified adhesive activity of peritoneal macrophages were examined via crystal violet staining by using a colorimetric method. ECS was modeled by the rotational movements of mandrel-knife inserted into the region of capsula interna (c.i.) on the left by guiding a needlecannula with the subsequent damage of vessels and the formation of mild (local incision of vessels in the region of c.i.), moderate (additional introduction of autologous blood in the area of c.i.), and severe (cutting of vessels from c.i. to pia mater with the subsequent introduction of autologous blood into the damaged area) hemorrhage. Data were evaluated in 72 h after different severities of ECS were modeled. RESULTS: In 72 h after ECS with different severities was modeled, the functional and spontaneous metabolic activity of splenic macrophages significantly decreased compared with those in the control group. The functional, spontaneous, and induced metabolic activity of peritoneal macrophages significantly reduced compared with those in the control group. The adhesive activity of peritoneal macrophages significantly increased compared with that of the control group. These disorders exacerbated in animal models with a more severe degree of stroke. CONCLUSION: In the acute period of ECS, the functional activity of macrophages decreased, whereas their adhesive activity increased. These phenomena enhanced in more severe models of stroke.

scholarly journals Hemostasis system condition and endothelium functions during various pathogenesis types of ischemic strokes in the acute and regenerative periods

2009 ◽  
Vol 8 (1(2)) ◽  
pp. 72-77 ◽  
Author(s):  
Ye. G. Smertina ◽  
S. V. Prokopenko ◽  
V. G. Ionova ◽  
M. M. Tanashyan ◽  
V. V. Potylitsina
Keyword(s):  
Vascular Wall ◽  
Lacunar Stroke ◽  
Pathological Changes ◽  
Acute Period ◽  
Endothelium Dysfunction ◽  
Hemostasis System ◽  
The Given

It is revealed, that pathological changes in the plasmatic and platelets-vascular part of a hemostasis are expressed to a lesser degree in the acute period of the lacunar stroke in comparison with a nonlacunar stroke. Safety of antithrombotic properties of a vascular wall in the given subgroup is confirmed. Dis - tinctions in the hemostasis profile of various pathogenesis types of strokes disappear in the early regenerative period of stroke, lasting haemostatic activation with hyperfirinogenemia, and increase in markers of thrombosis, strengthening of spontaneous platelet’s aggregation, as well as increase of level of a marker endothelium dysfunction remains with the expressed decrease in reactance endothelium.

Low Prevalence of Coagulation and Fibrinolytic Activation in Patients with Primary Untreated Cancer

1990 ◽  
Vol 64 (03) ◽  
pp. 361-364 ◽  
Author(s):  
P B Nanninga ◽  
A van Teunenbroek ◽  
C H N Veenhof ◽  
H R Büller ◽  
J W ten Cate
Keyword(s):  
Cancer Patients ◽  
Fibrinolytic System ◽  
Coagulation System ◽  
Control Group ◽  
Tumor Type ◽  
Anti Cancer ◽  
Coagulation Tests ◽  
Low Prevalence ◽  
Thrombin Antithrombin Iii Complex

SummaryThe prevalence of subclinical coagulation abnormalities greatly differs in the various studies due to selection of patients and differences in study design. We performed coagulation studies in 69 consecutive patients with primary untreated cancer of various origin. The control group consisted of 42 sex and age matched healthy volunteers. Plasma coagulation tests included thrombin-antithrombin-III-complex (TAT), plasmin-alpha2-antiplasmin-complex (PAP) and tissue-plasminogen-activator-antigen (t-PA-ag). These tests were performed once, prior to any anti-cancer treatment. We evaluated if activation of the coagulation system (elevated TAT-complexes) and the fibrinolytic system (elevated PAP-complexes and t-PA-ag) correlated with the tumor-type or the extent of the tumor. To document clinical manifest haemorrhage or thromboembolic disease (TED) we performed a 6 months follow-up study.In 8 patients (12%) and in 3 control subjects (7%) an elevated TAT-complex level was observed (this difference is not significant). An increased plasma level of PAP-complex was seen in 8 patients (12%) versus none in the control group (p <0.05). In one patient both TAT and PAP-complex concentrations were elevated. Consequently, 15 of the 69 patients (22%) showed activation of the coagulation and/or fibrinolytic system.Fibrinolysis seems to be enhanced in a subset of cancer patients in contrast to blood coagulation.In 10 patients (14%) we found raised t-PA-ag levels. Three patients had both elevated levels of PAP-complex and t-PA-ag. These findings suggest that in a minority of patients increased PAP-complex levels may be a result of t-PA induced plasminogen activation. No preferential association was found between tumortype and activation of these systems.During follow-up we encountered in only 4 patients (6%) clinical evidence of haemorrhage or TED.We conclude that a subclinical coagulopathy is present in approximately a fifth (22%, 95 percent confidence limits 13-33%) of untreated primary cancer patients.

The response of skin perfusion and of rheological and immunological variables to intravenous prostanoid administration in Raynaud’s phenomenon secondary to collagenosis

VASA ◽  
2005 ◽  
Vol 34 (4) ◽  
pp. 243-249 ◽  
Author(s):  
Drinda ◽  
Neumann ◽  
Pöhlmann ◽  
Vogelsang ◽  
Stein ◽  
...  
Keyword(s):  
Laser Doppler Flowmetry ◽  
Raynaud’S Phenomenon ◽  
Raynaud's Phenomenon ◽  
Plasma Viscosity ◽  
Laser Doppler ◽  
Erythrocyte Aggregation ◽  
Control Group ◽  
Long Term Effects ◽  
Doppler Flowmetry

Background: Prostanoids are used in the treatment of Raynaud’s phenomenon and acral perfusion disorders secondary to collagenosis. In subjective terms, intravenous administration of these agents produces success in more than 50% of patients. The therapeutic outcome of clinical administration of alprostadil or iloprost may vary from individual to individual. Patients and methods: The following variables were analysed in a cross-over study in 27 patients with collagenosis and Raynaud’s phenomenon: plasma viscosity and erythrocyte aggregation (rheological variables), partial pressure of oxygen and laser Doppler flowmetry in the finger region, and lymphocyte phenotyping and interleukin (IL) determinations (immunological variables). Results: Laser Doppler flowmetry revealed significant differences between patients with secondary Raynaud’s phenomenon and a control group of 25 healthy subjects. Laser Doppler readings did not change significantly as a result of the treatments. Therapy with iloprost produced a reduction in IL-1beta, L-selectin (CD 62 L) and IL-6. Conclusion: The change in immunological variables due to iloprost may explain the long-term effects of prostaglandins in the treatment of Raynaud’s phenomenon. From our results it is not possible to infer any preference for iloprost or alprostadil.

Acute and Chronic Changes in Platelet Volume and Count After Cardiopulmonary Bypass Induced Thrombocytopenia in Man

1987 ◽  
Vol 57 (01) ◽  
pp. 55-58 ◽  
Author(s):  
J F Martin ◽  
T D Daniel ◽  
E A Trowbridge
Keyword(s):  
Platelet Count ◽  
Mean Platelet Volume ◽  
Artery Bypass ◽  
Bypass Graft ◽  
Control Group ◽  
Artery Bypass Graft ◽  
Platelet Volume ◽  
Young Males ◽  
The Mean

SummaryPatients undergoing surgery for coronary artery bypass graft or heart valve replacement had their platelet count and mean volume measured pre-operatively, immediately post-operatively and serially for up to 48 days after the surgical procedure. The mean pre-operative platelet count of 1.95 ± 0.11 × 1011/1 (n = 26) fell significantly to 1.35 ± 0.09 × 1011/1 immediately post-operatively (p <0.001) (n = 22), without a significant alteration in the mean platelet volume. The average platelet count rose to a maximum of 5.07 ± 0.66 × 1011/1 between days 14 and 17 after surgery while the average mean platelet volume fell from preparative and post-operative values of 7.25 ± 0.14 and 7.20 ± 0.14 fl respectively to a minimum of 6.16 ± 0.16 fl by day 20. Seven patients were followed for 32 days or longer after the operation. By this time they had achieved steady state thrombopoiesis and their average platelet count was 2.44 ± 0.33 × 1011/1, significantly higher than the pre-operative value (p <0.05), while their average mean platelet volume was 6.63 ± 0.21 fl, significantly lower than before surgery (p <0.001). The pre-operative values for the platelet volume and counts of these patients were significantly different from a control group of 32 young males, while the chronic post-operative values were not. These long term changes in platelet volume and count may reflect changes in the thrombopoietic control system secondary to the corrective surgery.

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