New Multiplex PCR Method for Identification of East European Green Frog Species and Their Hybrids

2019 ◽  
Vol 26 (6) ◽  
pp. 367-370 ◽  
Author(s):  
O. Ermakov ◽  
A. Ivanov ◽  
S. Titov ◽  
A. Svinin ◽  
S. N. Litvinchuk
Keyword(s):  
Multiplex Pcr ◽  
Nuclear Dna ◽  
East European Plain ◽  
Green Frog ◽  
Oxidase Gene ◽  
East European ◽  
Frog Species ◽  
Identification Of Species ◽  
Pcr Method ◽  
Species Specific

A molecular multiplex PCR method for identification of East European green frog species (Pelophylax ridibundus, P. cf. bedriagae and P. lessonae) and their hybrids was developed. This simple and rapid method can be used for identification of species-specific mitochondrial and nuclear DNA. The method is based on species-specific differences in primary structure of the subunit 1 of the mitochondrial cytochrome C oxidase gene (COI) and the intron-1 of the nuclear serum albumin gene (SAI-1). Based on the method, we analyzed numerous individuals of these species and their hybrids from East European Plain, the Crimea, the Caucasus, the Ural, as well as introduced populations from Western Siberia and the Kamchatka. In all cases, identification of species performed by use of the multiplex PCR method coincided with results of study of primary nucleotide sequences.

scholarly journals Rapid Method for Species-Specific Identification and Determination of Toxigenicity of Vibrio Cholerae from Natural Aquatic Environment

1970 ◽  
Vol 1 (1) ◽  
pp. 69-75
Author(s):  
Bidhan Chakraborty ◽  
Khadiza Zaman ◽  
Md Majibur Rahman
Keyword(s):  
Multiplex Pcr ◽  
Vibrio Cholerae ◽  
Serological Test ◽  
Seasonal Pattern ◽  
Public Health Problem ◽  
Major Public Health Problem ◽  
Pcr Multiplex ◽  
Pcr Method ◽  
Species Specific

Cholera caused by toxigenic Vibrio cholerae is a major public health problem confronting developing countries, where outbreaks occur in a regular seasonal pattern and are particularly associated with poverty and poor sanitation. It is generally accepted that seven distinct pandemics of cholera have occurred since the onset of the first pandemic in 1817. Again Vibrio cholerae is capable of surviving in aquatic environments for extended periods and is considered as autochthonous species in estuarine and brackish waters. Therefore, the present study was designed to isolate V. cholerae from natural environmental samples subsequently identified by conventional and molecular biological techniques. A total number of 10 isolates were included randomly in this study based on their initial identification. The serotypes of the isolates were determined by serological test (slide agglutination) and the number of serotypes O1, O139 and non-O1/O139 were 3, 2 and 5 respectively which were reconfirmed by PCR method. Finally, the toxigenicity of the isolates was analyzed by multiplex PCR method and five (5) isolates were found to contain the ctx gene, the major virulence factor of V. cholerae. Key Words: Vibrio cholerae, Simplex PCR, Multiplex PCR, Serotypes, Toxigenicity.   doi:10.3329/sjps.v1i1.1811 S. J. Pharm. Sci. 1(1&2): 69-75

Detection of species specific DNA fragments of tiger and leopard source materials by multiplex PCR method

2010 ◽  
Vol 150 ◽  
pp. 127-127
Author(s):  
Jijuan Cao ◽  
Junyi Xu ◽  
Qiuyan Wang ◽  
Ran Liu ◽  
Chuanwei Hu ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Dna Fragments ◽  
Pcr Method ◽  
Species Specific ◽  
Source Materials

Development of rapid PCR method for simultaneous identification of species, specific capsular type, and toxigenicity of Pasteurella sp. isolates

2011 ◽  
Vol 21 (6) ◽  
pp. 1333-1336 ◽  
Author(s):  
Ilnaz Sahragard ◽  
Yahya Tahamtan ◽  
Majid Valadan ◽  
Masoumeh Hyati ◽  
Farid Moazeni ◽  
...  
Keyword(s):  
Capsular Type ◽  
Rapid Pcr ◽  
Identification Of Species ◽  
Pcr Method ◽  
Species Specific ◽  
Simultaneous Identification

scholarly journals Detection and Differentiation ofListeria spp. by a Single Reaction Based on Multiplex PCR

1999 ◽  
Vol 65 (10) ◽  
pp. 4688-4692 ◽  
Author(s):  
Andreas Bubert ◽  
Inge Hein ◽  
Marcus Rauch ◽  
Angelika Lehner ◽  
ByoungSu Yoon ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Dna Sequences ◽  
Simultaneous Detection ◽  
Specific Gene ◽  
Food Hygiene ◽  
Specific Detection ◽  
Pcr Method ◽  
Species Specific ◽  
Different Sources ◽  
Single Reaction

ABSTRACT The iap gene encodes the protein p60, which is common to all Listeria species. A previous comparison of the DNA sequences indicated conserved and species-specific gene portions. Based on these comparisons, a combination consisting of only five different primers that allows the specific detection and differentiation ofListeria species with a single multiplex PCR and subsequent gel analysis was selected. One primer was derived from the conserved 3′ end and is specific for all Listeria species; the other four primers are specific for Listeria monocytogenes,L. innocua, L. grayi, or the three grouped species L. ivanovii, L. seeligeri, and L. welshimeri, respectively. The PCR method, which also enables the simultaneous detection of L. monocytogenes and L. innocua, was evaluated against conventional biotyping with 200 food hygiene-relevant Listeria strains. The results indicated the superiority of this technique. Thus, this novel type of multiplex PCR may be useful for rapid Listeria species confirmation and for identification of Listeria species for strains isolated from different sources.

Geographical features of forest vegetation reflected at the level of the lower-rank syntaxa (evidence from the Russian part of the Upper Dnieper basin)

2017 ◽  
pp. 94-108 ◽  
Author(s):  
Yu. A. Semenishchenkov
Keyword(s):  
Forest Vegetation ◽  
East European Plain ◽  
Early 20Th Century ◽  
Suggested Approach ◽  
Central European ◽  
Classification Schemes ◽  
East European ◽  
Lower Rank ◽  
Geographical Variations ◽  
European Part Of Russia

Phytogeographical features of forest vegetation at the level of lower-rank syntaxa were being discussed in literature since the early 20th century (Cajander, 1903; Sukachev, 1926; Braun-Blanquet, 1964; Kral et al., 1975; Kleopov, 1990; Bulokhov, 2003; Ellenberg, 2009), however, phytocoenologists still have no uniform interpretation and geographical maintenance of lower classification units. Forest vegetation of the European part of Russia is well studied according to Braun-Blanquet approach with association as a system of geographical subassociations. The paper offers the approaches to the reflection of geographical variations of the natural forest vegetation in the basin of the Upper Dnieper (central part of the East European Plain) at the level of lower-rank syntaxa The xeromesophytic oak woods in the basin of the Upper Dnieper belong to the East European ass. Lathyro nigri–Quercetum roboris Bulokhov et Solomeshch 2003. Floristic differentiation of this association from the similar Central European ass. Potentillo-Quercetum is given. These two associations have large blocks of geographically significant differential species that does not allow to consider them as a part of one association. The suggested approach allows to define the chorological content of units of lower syntaxonomical ranks and make regional classification schemes comparable to each other.

scholarly journals Eastern Europe’s “Transitional Industry”?: Deconstructing the Early Streletskian

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
R. Dinnis ◽  
A. Bessudnov ◽  
N. Reynolds ◽  
T. Devièse ◽  
A. Dudin ◽  
...  
Keyword(s):  
Modern Human ◽  
East European Plain ◽  
Upper Palaeolithic ◽  
Radiocarbon Dates ◽  
East European ◽  
Archaeological Material ◽  
Younger Age ◽  
Layer V ◽  
Precise Relationship ◽  
Early Upper

AbstractThe Streletskian is central to understanding the onset of the Upper Palaeolithic on the East European Plain. Early Streletskian assemblages are frequently seen as marking the Neanderthal-anatomically modern human (AMH) anthropological transition, as well as the Middle-to-Upper Palaeolithic archaeological transition. The age of key Streletskian assemblages, however, remains unclear, and there are outstanding questions over how they relate to Middle and Early Upper Palaeolithic facies. The three oldest Streletskian layers—Kostenki 1 Layer V, Kostenki 6 and Kostenki 12 Layer III—were excavated by A. N. Rogachev in the mid-20th century. Here, we re-examine these layers in light of problems noted during Rogachev’s campaigns and later excavations. Layer V in the northern part of Kostenki 1 is the most likely assemblage to be unmixed. A new radiocarbon date of 35,100 ± 500 BP (OxA- X-2717-21) for this assemblage agrees with Rogachev’s stratigraphic interpretation and contradicts later claims of a younger age. More ancient radiocarbon dates for Kostenki 1 Layer V are from areas lacking diagnostic Streletskian points. The Kostenki 6 assemblage’s stratigraphic context is extremely poor, but new radiocarbon dates are consistent with Rogachev’s view that the archaeological material was deposited prior to the CI tephra (i.e. >34.3 ka BP). Multiple lines of evidence indicate that Kostenki 12 Layer III contains material of different ages. Despite some uncertainty over the precise relationship between the dated sample and diagnostic lithic material, Kostenki 1 Layer V (North) therefore currently provides the best age estimate for an early Streletskian context. This age is younger than fully Upper Palaeolithic assemblages elsewhere at Kostenki. Other “Streletskian” assemblages and Streletskian points from younger contexts at Kostenki are briefly reviewed, with possible explanations for their chronostratigraphic distribution considered. We caution that the cultural taxon Streletskian should not be applied to assemblages based simply on the presence of bifacially worked artefacts.

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