scholarly journals COMPOSITION OF LEUKOCYTES IN BLOOD OF GOLDFISH INFECTED BY LIGULA (DIGRAMMA) INTERRUPTA (CESTODA: PSEUDOPHYLLIDEA)

2020 ◽  
pp. 207-212
Author(s):  
Mazur ◽  
Kutyrev ◽  
Dugarov

The tapeworm Ligula interrupta of the genus Ligula (Digramma) (Cestoda: Pseudophyllidea) is a specific parasite of freshwater cyprinid fish; its plerocyrcoids are localized in the body cavity causing heavy parasitic pathology. With parasitosis, an important role in the regulation of the pathological process is assigned to the participants of the organism’s protective reactions, among which a special pool of immunocompetent cells – leukocytes – is distinguished. In the literature, there are single data on hemo- and immunopoiesis goldfish with digrammosis. In our work, new data were obtained on the leukocyte composition of the blood of goldfish Carassius auratus (Linnaeus, 1758), infected with Ligula interrupta in conditions of the native range (Shilen Lake and Cheremukhovoe Lake, Lake Baikal basin). All fish specimens caught with seine nets, both infested and noninfested with L. interrupta, were identical by the size and age composition (age +3…+5, body length 145–237 and 150–180 mm, respectively, weight 100–184 and 120–174 g). Hematological studies were carried out according to the Sbornik [2]. To quantify the individual types of leukocytes, the leukocyte profile, expressed in absolute terms, was calculated. Three groups of cells were revealed in the blood of all goldfish species: blast forms, granulocytes (neutrophils, pseudobasophils) and agranulocytes (lymphocytes, monocytes). The decrease in the blood of infected fish of the total number of leukocytes and their various types at all stages of development, the violation of the proliferation and differentiation of lymphoid and granulocytic elements in the studied organs, the formation of vacuolated and atypical cells, the absence of circulating basophils and eosinophils were established. It has been shown that in this parasitic system, partner relations are reduced to the launch of L. interrupta mechanisms that regulate the host's protective immunological processes by the type of inhibition of the acute inflammatory response and the development of mild chronic inflammation.

Behaviour ◽  
1995 ◽  
Vol 132 (15-16) ◽  
pp. 1223-1240 ◽  
Author(s):  
Iain Barber ◽  
Felicity A. Huntingford

AbstractIn this paper we review recent experimental work on the effects of the parasite Schistocephalus solidus (Cestoda: Pseudophyllidea) on the feeding behaviour of three-spined sticklebacks (Gasterosteus aculeatus L.). We also discuss how increased feeding motivation and subsequent altered foraging behaviour may be a mechanism for parasite-associated changes in the shoaling behaviour of infected sticklebacks. The presence of S. solidus plerocercoids in the body cavity constricts the stomach, increases the handling time for large prey and consequently reduces the profitability of such prey for infected fish. This is reflected in a switch in dietary preference from large to small prey in the laboratory and in altered stomach contents and impaired nutrient reserves in the wild. By altering their hosts' nutritional state by direct competition for nutrients from digested food (and possibly indirectly by altering diet and reducing competitive ability) and also by altering the fishes' appearance, such parasites have the potential to alter the costs and benefits involved in joining a shoal of conspecifics. Experimental work on the shoaling decisions of S. solidus-infected sticklebacks supports this hypothesis, and such behavioural modification is discussed in the context of the manipulation hypothesis of parasite transmission.


1971 ◽  
Vol 36 (2) ◽  
pp. 200-206 ◽  
Author(s):  
Louise M. Robbins

AbstractA mummy, or rather, a dessicated body, from Salts Cave, Kentucky, has been subjected to a comprehensive analysis. Radiocarbon analysis of internal body tissue placed the individual in the Woodland cultural period. A careful dissection of the body cavity revealed the presence of all internal organs but, due to dehydration, in a collapsed form. The diet of the individual, and indirectly of the population, was determined from the contents of the fecal material in the lower intestine and colon. Examination of the external side of the body revealed the individual to be a male, and radiographic and dental evidence indicated that he was around nine years of age. Blood group studies are still somewhat inconclusive, but tests are being conducted to clarify that problem. This paper was originally presented at the 35th Annual Meeting of the Society for American Archaeology, Museo Nacional de Antropologia, Mexico, D.F.


Reproduction ◽  
2005 ◽  
Vol 130 (6) ◽  
pp. 939-945 ◽  
Author(s):  
V Carter ◽  
R Pierce ◽  
S Dufour ◽  
C Arme ◽  
D Hoole

The tapewormLigula intestinalisoccurs in the body cavity of its cyprinid second intermediate host, in this study the roachRutilus rutilus, and inhibits host gonadal development. The mechanism by which infected fish are prevented from reproducing is unknown. Comparison of parameters, such as body length and weight, and condition factor and age, between infected and uninfected individuals, indicated only minor effects of parasitism on growth and condition. In contrast, seasonal gonadal development, as observed in uninfected fish, did not occur in infected fish, and gonads remained small and blocked at the primary oocyte stage in female roach. As immature ovaries and testes are still present, the parasite is presumed to act upon the brain–pituitary–gonadal axis of the fish to inhibit further development of reproductive organs. We investigated theLigula/fish interaction at the level of the pituitary gland by determination of gonadotrophin (LH) content using a heterologous RIA for carp (Cyprinus carpio) LHβ subunit. The results indicated that the pituitary glands of infected roach contained approximately 50% less LH than non-infected fish. After the cloning and sequencing of roach LHβ subunit, we measured roach LHβ mRNA levels by real-time RT-PCR. A corresponding 50% reduction in LHβ mRNA pituitary levels was determined. These results reflect a significant and measurable effect of parasitism on the pituitary gland, and lend support to the hypothesis that excretory/secretory products released from the parasite interact with the brain–pituitary–gonadal axis of the fish host and thus inhibit gonadal development.


2021 ◽  
pp. 3-18
Author(s):  
Andrii Antonov ◽  
Mykola Klymenko ◽  
Serhii Tatarko ◽  
Oleksandr Herasymenko ◽  
Natalia Surhai ◽  
...  

The aim of the study is detailed analysis of the literature on the existing methods for determining the lifetime and inflicting of mechanical damage. It was found that the existing methods have very large discrepancies in assessing the infliction of damage. The use of the same research methods by different researchers has lead to results that may differ. This can lead to obtaining insufficiently substantiated or even erroneous conclusions about the infliction of mechanical damage. The authors explain this by the fact that the body reacts to damage by the development of a typical pathological process – inflammation. Forensic methods that exist for determining the age of wound are aimed at obtaining an expert assessment of individual manifestations of the pathogenesis of inflammation by morphological, biochemical and immunological methods. Determination of the age of wound is actually the determination of a specific stage in the pathogenesis of inflammation, which is observed at the time of the examination and the timing of the development, which is known from the literature. Taking into account the stages of the pathogenesis of inflammation as a typical pathological process, in the area of tissue damage makes it possible to more accurately determine the duration of the infliction of mechanical damage. The individual reactivity of the organism, which determines the features of the course of any typical pathological process, will determine the individual timing of the development of certain morphological, biochemical and immunological changes in the area of damage.  


2001 ◽  
Vol 40 (01) ◽  
pp. 31-37 ◽  
Author(s):  
U. Wellner ◽  
E. Voth ◽  
H. Schicha ◽  
K. Weber

Summary Aim: The influence of physiological and pharmacological amounts of iodine on the uptake of radioiodine in the thyroid was examined in a 4-compartment model. This model allows equations to be derived describing the distribution of tracer iodine as a function of time. The aim of the study was to compare the predictions of the model with experimental data. Methods: Five euthyroid persons received stable iodine (200 μg, 10 mg). 1-123-uptake into the thyroid was measured with the Nal (Tl)-detector of a body counter under physiological conditions and after application of each dose of additional iodine. Actual measurements and predicted values were compared, taking into account the individual iodine supply as estimated from the thyroid uptake under physiological conditions and data from the literature. Results: Thyroid iodine uptake decreased from 80% under physiological conditions to 50% in individuals with very low iodine supply (15 μg/d) (n = 2). The uptake calculated from the model was 36%. Iodine uptake into the thyroid did not decrease in individuals with typical iodine supply, i.e. for Cologne 65-85 μg/d (n = 3). After application of 10 mg of stable iodine, uptake into the thyroid decreased in all individuals to about 5%, in accordance with the model calculations. Conclusion: Comparison of theoretical predictions with the measured values demonstrated that the model tested is well suited for describing the time course of iodine distribution and uptake within the body. It can now be used to study aspects of iodine metabolism relevant to the pharmacological administration of iodine which cannot be investigated experimentally in humans for ethical and technical reasons.


2020 ◽  
Vol 11 (SPL1) ◽  
pp. 1477-1481
Author(s):  
Ishwari Gaikwad ◽  
Priyanka Shelotkar

The current world situation is both frightening and alarming due to the massive disruption caused by the Covid-19 pandemic. The next few days are censorious as we need to be very precautious in our daily regimen as well as dietary habits. Ayurveda offers knowledge about food based on certain reasoning. Indecent food custom is the chief cause for the rising development of health disorders in the current era. In classical texts of Ayurveda, the concept of diet explained well, ranging from their natural sources, properties and specific utility in pathological as well as physiological manner. In this work, the review of the relevant literature of Ahara (Diet) was carried out from Charak Samhita and other texts, newspapers, articles, web page related to the same.  Every human being is unique with respect to his Prakriti (Physical and mental temperament), Agni (Digestive capacity), Koshtha  (Nature of bowel) etc. For that reason, the specificity of the individual should be kept in mind. Ahara, when consumed in the appropriate amount at the right moment following all Niyamas (Guidelines) given in Ayurveda texts, gives immunity and keeps the body in a healthy state during pandemics such as Covid-19. Ultimately, this will help the human body to maintain its strength for life. This article reviews the concept of diet viz. combination of foods, their quantity and quality, methods of preparation and processing, which are to be followed during pandemics and are essential in maintenance and endorsement of health and preclusion of diseases.


2020 ◽  
Vol 5 (5) ◽  
pp. 386-393
Author(s):  
L. M. Gunina ◽  
◽  
Kazys Mylashyus ◽  
Voitenko V. L. ◽  
◽  
...  

Under high-intensity loads, the athlete's bodies take place a number of biochemical reactions and physiological processes that can lead to hyperbilirubinemia. The factors that can initiate the onset of this phenomenon include the syndrome of micro-damage muscle, violation of the integrity of erythrocyte membranes, decreased blood pH, malnutrition and increase oxygen demand of the body. Degree of expression of manifestations of physiological bilirubinemia depends on the level of adaptation of the athlete to the physical activities offered. Hyperbilirubinemia in athletes can be one of the components of the deterioration of the functional state, forming the symptoms of endogenous intoxication. The relevance of this problem in sport lies in the relatively low detection rate of hyperbilirubinemia due to the lack of regular screening studies. However, in drawing up a plan of nutritional- metabolic support for training and competitive activity and recovery measures, must not only the individual reaction of the athlete body to physical activity, but also the severity of shifts in the indicators of bilirubin metabolism and their ratio. The article describes the reasons for the increase in bilirubin levels, which can be caused by both the effect of physical activity and by the presence of pathological processes in athletes. The factors influencing the blood serum’s bilirubin content are also highlighted, which include the state of erythrocyte cell membranes and the rate of hemoglobin destruction, the functional state of the liver, the specifics of physical loads and the use of ergogenic pharmacological agents by athletes. Particular accent has been placed on the illumination of hereditary hyperbilirubinemias, which may have been detected at the stage of selection of athletes. The most common phenomenon is Gilbert's syndrome, which occurs in 2-5% of cases in the general population, is characterized in the clinic by a benign flow and is manifested by episodes of jaundice and an increase in total bilirubin content to moderate values due to indirect. The frequency of detection of hyperbilirubinemias in the population of athletes is 4.68%, among which Gilbert's disease accounts for almost half (48.7%). Conclusion. The work highlighted the pathogenesis and diagnostic algorithm of Gilbert's disease, and also emphasized that its drug prevention and correction in athletes to maintain functional and physical fitness should be carried out taking into account anti-doping rules, which requires upon diagnosis timely receipt of a therapeutic exclusion


Author(s):  
Rajendra Pai N. ◽  
U. Govindaraju

Ayurveda in its principle has given importance to individualistic approach rather than generalize. Application of this examination can be clearly seem like even though two patients suffering from same disease, the treatment modality may change depending upon the results of Dashvidha Pariksha. Prakruti and Pramana both used in Dashvidha Pariksha. Both determine the health of the individual and Bala (strength) of Rogi (Patient). Ayurveda followed Swa-angula Pramana as the unit of measurement for measuring the different parts of the body which is prime step assessing patient before treatment. Sushruta and Charaka had stated different Angula Pramana of each Pratyanga (body parts). Specificity is the characteristic property of Swa-angula Pramana. This can be applicable in present era for example artificial limbs. A scientific research includes collection, compilation, analysis and lastly scrutiny of entire findings to arrive at a conclusion. Study of Pramana and its relation with Prakruti was conducted in 1000 volunteers using Prakruti Parkishan proforma with an objective of evaluation of Anguli Pramana in various Prakriti. It was observed co-relating Pramana in each Prakruti and Granthokta Pramana that there is no vast difference in measurement of head, upper limb and lower limb. The observational study shows closer relation of features with classical texts.


Author(s):  
Joshua S. Walden

The book’s epilogue explores the place of musical portraiture in the context of posthumous depictions of the deceased, and in relation to the so-called posthuman condition, which describes contemporary changes in the relationship of the individual with such aspects of life as technology and the body. It first examines Alfred Hitchcock’s Vertigo to view how Bernard Herrmann’s score relates to issues of portraiture and the depiction of the identity of the deceased. It then considers the work of cyborg composer-artist Neil Harbisson, who has aimed, through the use of new capabilities of hybridity between the body and technology, to convey something akin to visual likeness in his series of Sound Portraits. The epilogue shows how an examination of contemporary views of posthumous and posthuman identities helps to illuminate the ways music represents the self throughout the genre of musical portraiture.


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