scholarly journals Calendula officinalis L. bitkisinin morfolojik özellikleri, ekstre içeren topikal ilaç formülasyonu geliştirilmesi ve in vitro değerlendirilmesi

2022 ◽  
Author(s):  
Umay Merve GÜVEN ◽  
Selen ARSLAN ◽  
Melike Betül ÇIRACI ◽  
Serpil DEMİRCİ KAYIRAN
Keyword(s):  
Calendula Officinalis ◽  
Calendula Officinalis L

scholarly journals From the Field to the Pot: Phytochemical and Functional Analyses of Calendula officinalis L. Flower for Incorporation in an Organic Yogurt

Antioxidants ◽  
2019 ◽  
Vol 8 (11) ◽  
pp. 559 ◽  
Author(s):  
Bragueto Escher ◽  
Cardoso Borges ◽  
Sousa Santos ◽  
Mendanha Cruz ◽  
Boscacci Marques ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Biological Activities ◽  
Total Phenolic ◽  
Antioxidant Compounds ◽  
Calendula Officinalis ◽  
Antioxidant Power ◽  
Ferric Reducing Antioxidant Power ◽  
Functional Analyses ◽  
Calendula Officinalis L

Edible flowers have been used as ingredients because of their biological activities, taste, and overall appearance. This research was aimed to characterize the chemical composition and in vitro antioxidant activity of the marigold flower (Calendula officinalis L.) extracted with different proportions of water and ethyl alcohol, and the lyophilized extract with higher content of antioxidant compounds was incorporated into an organic yogurt. Results showed that the hydroalcoholic extract (50:50 v/v) presented the highest total phenolic content (TPC), flavonoids, and antioxidant activity (ferric reducing antioxidant power (FRAP), total reducing capacity (TRC), and Cu2+/Fe2+ chelating ability). Phenolic acids and flavonoids were quantified in the extract by LC-DAD, while 19 compounds were tentatively identified by ESI-MS/MS. The lyophilized marigold extract (LME) also inhibited 12% of Wistar rat’s brain lipid oxidation in vitro, inhibited α-amylase, and α-glucosidase activities, but showed no cytotoxicity towards cancerous cells (HCT8 and A549). However, marigold flower extract protected human erythrocytes against mechanical stress. When added into an organic yogurt model (0 to 1.5%), LME increased TPC and antioxidant activity (2,2-diphenyl-1-picrylhydrazyl (DPPH) and TRC), and the sensory analysis showed that the organic yogurt had an acceptance of 80.4%. Our results show that the use of LME may be a technological strategy to increase the content of bioactive compounds in yogurts.

scholarly journals Efeito cicatrizante e atividade antibacteriana da Calendula officinalis L. cultivada no Brasil

2009 ◽  
Vol 11 (4) ◽  
pp. 383-391 ◽  
Author(s):  
L.M.L. Parente ◽  
M.S.B. Silva ◽  
L.A.B. Brito ◽  
R.S. Lino-Júnior ◽  
J.R. Paula ◽  
...  
Keyword(s):  
Calendula Officinalis ◽  
Calendula Officinalis L

Desde a antiguidade propriedades medicinais são atribuídas às flores da Calendula officinalis L. (Asteraceae) destacando-se a atividade cicatrizante. Estudos sobre a atividade geral de plantas medicinais na cicatrização vêm sendo realizados, sem especificar sobre qual das fases da cicatrização a planta atua. Neste trabalho a atividade cicatrizante e antiinflamatória do extrato etanólico das flores da C. officinalis cultivada no Brasil foi avaliada em feridas cutâneas de ratos Wistar, por meio de avaliação macroscópica e histológica. A atividade antimicrobiana do extrato e das frações hexânica e diclorometano também foi avaliada. A atividade antiinflamatória do extrato etanólico da calêndula foi atribuída à diminuição da exsudação serosa, da hiperemia, da deposição de fibrina e da hiperplasia epidermal, além de resultar em crostas mais delgadas e umedecidas. Observaram-se também aumento de colágeno no tecido de granulação e efeito antibacteriano. Assim, o extrato etanólico da calêndula atuou de forma positiva sobre a atividade cicatricial em feridas cutâneas de ratos, bem como apresentou atividade antibacteriana in vitro.

scholarly journals Inibição do crescimento micelial de Cercospora calendulae Sacc. por extratos de plantas medicinais

2013 ◽  
Vol 15 (4 suppl 1) ◽  
pp. 751-756 ◽  
Author(s):  
J.M. Nascimento ◽  
A.P. Serra ◽  
L.M. Bacchi ◽  
W.L. Gavassoni ◽  
M.C. Vieira
Keyword(s):  
Momordica Charantia ◽  
Ruta Graveolens ◽  
Calendula Officinalis ◽  
Ageratum Conyzoides ◽  
Mato Grosso ◽  
Symphytum Officinale ◽  
Calendula Officinalis L ◽  
Mato Grosso Do Sul

O uso de extratos e óleos essenciais de plantas medicinais tem sido amplamente estudado no controle de doenças de plantas. O objetivo da realização do presente trabalho foi avaliar o efeito de extratos de Ruta graveolens L., Mentha x villosa, Calendula officinalis L., Momordica charantia L., Symphytum officinale L., Ageratum conyzoides L. e Ricinus comunis L., nas concentrações de 0, 250, 500, 1000, 2000, 5000 e 10000 mg L-1, sobre a inibição do crescimento micelial de Cercospora calendulae Sacc. in vitro. Os extratos foram obtidos por infusão. O experimento foi desenvolvido no Laboratório de Fitopatologia da FCA/UFGD, estado de Mato Grosso do Sul, em delineamento inteiramente casualizado, esquema fatorial 7 extratos x 7 concentrações, com seis repetições. Foi detectado efeito dos extratos e suas concentrações sobre o crescimento do fungo, sendo a interação significativa. Os extratos de calêndula, arruda, hortelã e melão de São Caetano, nas maiores concentrações resultaram em maiores porcentagens de inibição, próximas de 100%, 30%, 35% e 40%, respectivamente, a 10000 mg L-1.

scholarly journals Cytotoxicological evaluation of semi-purified extracts of some dye yielding plants of the Kashmir Valley on Normal Intestinal Cell Line (IEC-6) by MTT assay

2018 ◽  
Vol 7 (1) ◽  
pp. 5-9
Author(s):  
Qazi Gazala ◽  
◽  
Shoukat Ara ◽  
KM Ansari ◽  
Imtiyaz Murtaza ◽  
...  
Keyword(s):  
Cell Line ◽  
Plant Extracts ◽  
Mtt Assay ◽  
Cytotoxic Effect ◽  
Intestinal Cell ◽  
Calendula Officinalis ◽  
Rubia Cordifolia ◽  
Intestinal Cell Line ◽  
Calendula Officinalis L

Plant extracts are widely used in many fields and there is a need to evaluate their cytotoxic effect to determine their non-cytotoxic concentration at which they can be used in a safe manner. Keeping this in view, the present study was designed to evaluate the in vitro toxicity of Celosia argentia L. var plumosa (Cockscomb), Calendula officinalis L. (Pot Marigold), Indigofera heterantha Wall. (Himalayan Indigo) and Rubia cordifolia L. (Indian Madder) on Normal Intestinal Cell Line (IEC-6) by MTT assay to test their feasibility for natural edible dye extraction. The experimental material, comprised of inflorescence of Celosia argentia L. var plumose, petals of the two varieties of Calendula officinalis L., leaves of Indigofera heterantha Wall. and leaves and roots of the Rubia cordifolia L. Cell line was exposed to 1, 4, 16, 64 and 256µg/ml concentrations of plant extracts for 24, 48, and 72hr at 37oC. Results revealed that both the varieties of Calendula officinalis L. var. Gitana Orange and Gitana Yellow did not show any cytotoxic effect on IEC-6 cell line while as Celosia argentia L. var plumose, Indigofera heterantha Wall. and Rubia cordifolia L. showed cytotoxicity. From the present study it was concluded that the extracts of the both varieties of Calendula officinalis L. var. Gitana Orange and Gitana Yellow extracts are non-toxic in nature, thus can be utilized for the extraction of natural edible dye while as the extracts of Celosia argentia L. var plumose, Indigofera heterantha Wall. and Rubia cordifolia L. had potent in vitro cytotoxic activity thus they cannot be used for extraction of natural edible food colour. However, to better evaluate the cytotoxic effect of these plant extracts, in vivo experiments on laboratory animal followed by histological analysis should be done

scholarly journals The effect of Apium Graveolens L., Levisticum Officinale and Calendula Officinalis L. on cell viability, membrane integrity, steroidogenesis, and intercellular communication in mice Leydig cells in vitro

2021 ◽  
pp. 615-625
Author(s):  
Tomas Jambor ◽  
Julius Arvay ◽  
Eva Tvrda ◽  
Anton Kovacik ◽  
Hana Greifova ◽  
...  
Keyword(s):  
Cell Membrane ◽  
Steroid Hormones ◽  
Leydig Cell ◽  
Leydig Cells ◽  
Membrane Integrity ◽  
Apium Graveolens ◽  
Calendula Officinalis ◽  
Cell Membrane Integrity ◽  
Calendula Officinalis L

Several plants have the potential to protect essential reproductive processes such as spermatogenesis or steroidogenesis, however, effective concentrations and main mechanisms of action are still unknown. This in vitro study was aimed to assess the effects of Apium graveolens L., Levisticum officinale, and Calendula officinalis L. extracts on the structural integrity, functional activity and gap junctional intercellular communication (GJIC) in mice Leydig cells. TM3 cells were grown in the presence of experimental extracts (37.5, 75, 150 and 300 µg/ml) for 24 h. For the present study, high-performance liquid chromatography analysis was used to quantify flavonoids or phenolic acids. Subsequently, Leydig cell viability was assessed by alamarBlue assay, while the cell membrane integrity was detected by 5 carboxyfluorescein diacetate-acetoxymethyl ester. The level of steroid hormones production was determined by enzyme-linked immunosorbent assay. Additionally, GJIC was assessed by scalpel loading/dye transfer assay. According to our results, Apium graveolens L. significantly increased the viability and cell membrane integrity at 75 µg/ml (109.0±4.3 %) followed by a decline at 300 µg/ml (89.4±2.3 %). In case of Levisticum officinale and Calendula officinalis L. was observed significant decrease at 150 µg/ml (88.8±11.66 %, 87.4±6.0 %) and 300 µg/ml (86.2±9.3 %, 84.1±4.6 %). Furthermore, Apium graveolens L. significantly increased the progesterone and testosterone production (75 and 150 µg/ml) however, Levisticum officinale and Calendula officinalis L. significantly reduced steroid hormones synthesis at 150 and 300 µg/ml. Finally, the disturbance of GJIC was significantly affected at 300 µg/ml of Levisticum officinale (82.5±7.7 %) and Calendula officinalis L. (79.8±7.0 %). The balanced concentration ratio may support the Leydig cell function, steroidogenesis as well as all essential parameters that may significantly improve reproductive functions.

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