scholarly journals Effect of Amoxicillin/Aqueous Extract of Cussonia arborea Hochst. (Araliaceae) on the Survival of Escherichia coli and Staphylococcus aureus Bacteria Isolated from Groundwater in Yaoundé (Central Region of Cameroon)

2022 ◽  
Vol 5 (1) ◽  
pp. 116-127
Author(s):  
Luciane Marlyse Moungang ◽  
Christophe Colombe Fotso Simo ◽  
Antoine Arfao Tamsa ◽  
Sophia Loeticia Ngoumnaï ◽  
Olive Vivien Ewoti Noah ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Aqueous Extract ◽  
Central Region

scholarly journals Presence and characterization of Escherichia coli virulence genes isolated from diseased pigs in the central region of Argentina

2017 ◽  
Vol 10 (8) ◽  
pp. 939-945 ◽  
Author(s):  
Fernando A. Bessone ◽  
Gabriela Bessone ◽  
Sebastian Marini ◽  
Maria B. Conde ◽  
Fabrisio E. Alustiza ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Central Region ◽  
Virulence Genes

scholarly journals Evaluation du potentiel antimicrobien et de la toxicité des extraits de Jatropha multifida Linn, (Euphorbiaceae)

2020 ◽  
Vol 151 ◽  
pp. 15550-15558
Author(s):  
Amégninou Agban ◽  
Yao Hoekou ◽  
Passimna Pissang ◽  
Tchadjobo Tchacondo ◽  
Komlan Batawila
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Aqueous Extract ◽  
E Coli ◽  
Toxicological Studies ◽  
Jatropha Multifida

Objectif : L’objectif de ce travail était d’évaluer in vitro l’activité antimicrobienne des extraits de feuilles et tige de Jatropha multifida sur la croissance de Candida albicans, Escherichia coli et Staphylococcus aureus, puis d’évaluer in vivo la toxicité de cette plante. Méthodologie et résultats : Les méthodes de diffusion en milieu gélosé et de microdilution en milieu liquide ont été utilisées pour évaluer l’effet antimicrobien. Une étude en subaigüe était réalisée afin d’explorer les effets toxiques de l’extrait aqueux des feuilles. Les résultats des tests antimicrobiens montrent une activité des extraits de feuilles et tige de J. multifida sur la croissance des souches utilisées avec des diamètres de zones d’inhibition allant de 8 à 25 mm et des concentrations minimales inhibitrices (CMI) variant de 0,039 mg/mL à 1,25 mg/mL à l’exception des souches de E. coli qui sont résistantes aux extraits de la tige. L’administration en subaigüe de l’extrait aqueux des feuilles de J. multifida à la dose de 600 mg/kg entraîne une perte significative de poids chez les souris. Conclusion et applications des résultats : Les extraits aqueux, éthanolique et hydroéthanolique des feuilles et tige de J. multifida possèdent d’activité antimicrobienne et pourraient être utilisés dans le traitement des Candidoses à C. albicans et des infections à S. aureus. Mais l’essai de toxicité subaigüe montre que l’extrait aqueux de la plante serait toxique. Des études toxicologiques approfondies restent donc nécessaires sur ces extraits afin de mieux élucider leur inocuité. Mots-clés : Jatropha multifida, extraits de feuilles et de tige, activités antifongique et antibactérienne, toxicité. Agban et al., J. Appl. Biosci. 2020 Evaluation du potentiel antimicrobien et de la toxicité des extraits de Jatropha multifida Linn, (Euphorbiaceae) 15551 Evaluation of antimicrobial potential and toxicity of Jatropha multifida Linn, (Euphorbiaceae) extracts ABSTRACT Objective: The objective of this study was to evaluate in vitro the antimicrobial activity of leaves and stem of Jatropha multifida extracts against Candida albicans, Escherichia coli and Staphylococcus aureus, and then to evaluate in vivo the toxicity of this plant. Methodology and Results: The agar well-diffusion and the NCCLS broth microdilution methods were used to assess the antimicrobial effect. A subacute study was carried out to explore the toxic effects of the aqueous extract of the leaves. The results of the antimicrobial tests show an activity of the extracts of leaves and stems of J. multifida on the growth of the strains used with diameters of inhibitory zones ranging from 8 to 25 mm and minimum inhibitory concentrations (MIC) varying from 0.039 mg/mL to 1.25 mg/mL exception E. coli strains which are resistant to extracts from the stem. Subacute administration of the aqueous extract of the leaves of J. multifida at a dose of 600 mg/kg leads to a significant loss of weight in the mice. Conclusion and application of findings : The aqueous, ethanolic and hydroethanolic extracts of the leaves and stem of J. multifida have antimicrobial activity and could be used in the treatment of Candidiasis and bacterial infections due respectively to C. albicans and S. aureus. But the subacute toxicity test shows that the aqueous extract of the plant would be toxic. Extensive toxicological studies therefore remain necessary on these extracts in order to better elucidate their safety. Keywords: Jatropha multifida extracts of leaves and stem, antifungal and antibacterial activities, toxicity

Phytochemical Content, Radical Scavenging and Antibacterial Properties of Aqueous Extract of Jatropha curcas Linn Leaves

2012 ◽  
Vol 1 (1) ◽  
Author(s):  
O B Oloyede
Keyword(s):  
Escherichia Coli ◽  
Hydrogen Peroxide ◽  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Aqueous Extract ◽  
Jatropha Curcas ◽  
Radical Scavenging ◽  
Klebsiella Pneumonia ◽  
Phytochemical Content ◽  
Proteus Species

This study investigated phytochemical content, radical scavenging and antibacterial activities of aqueous extract of leaves of Jatropha curcas Linn. Quantitative phytochemical analyses of alkaloids, phenols, tannins and flavonoids contents were carried out; radical scavenging activity was assessed using 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical and hydrogen peroxide inhibition assays while Disc diffusion and Agar well (ditch) diffusion methods were used for antibacterial activity against Klebsiella pneumonia, Escherichia coli, Pseudomonas aeruginosa, Staphilococcus aureus and Proteus species at 62.5, 125, 250 and 500 mg/mL. The extract contained alkaloids (1.600± 0.58 %), tannins (0.121 ± 0.00 mg/mL), phenols (0.463 ± 0.06 mg/mL) and flavonoids (0.672 ± 0.00 mg/mL), and showed radical scavenging activities against DPPH (IC50 = 21.24) and hydrogen peroxide (15.67 mg/mL) which were less than that of Butylated hydroxyanisole (BHA); IC50 = 3.92 (DPPH) and 6.19 mg/mL (hydrogen peroxide) respectively. It also showed antibacterial activity against Klebsiella pneumonia, Escherichia coli, and Pseudomonas aeruginosa at 250 and 500 mg/ml (MIC = 125 mg/mL), which were resistant to ampicillin, chloxacillin and erythromycin, and the extract was inactive against Staphilococcus aureus and Proteus species at these concentrations, though all were sensitive to gentamycin. This shows that the aqueous extract of leaves of Jatropha curcas Linn may possess some of the folkloric properties claimed.

scholarly journals Characterization of an Exo-β-d-Glucosaminidase Involved in a Novel Chitinolytic Pathway from the Hyperthermophilic Archaeon Thermococcus kodakaraensis KOD1

2003 ◽  
Vol 185 (17) ◽  
pp. 5175-5181 ◽  
Author(s):  
Takeshi Tanaka ◽  
Toshiaki Fukui ◽  
Haruyuki Atomi ◽  
Tadayuki Imanaka
Keyword(s):  
Escherichia Coli ◽  
Central Region ◽  
Primary Structure ◽  
Glycosyl Hydrolase ◽  
Chitinase Gene ◽  
Glycosyl Hydrolases ◽  
Galactosidase Activity ◽  
Hyperthermophilic Archaeon ◽  
Candidate Protein

ABSTRACT We previously clarified that the chitinase from the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1 produces diacetylchitobiose (GlcNAc2) as an end product from chitin. Here we sought to identify enzymes in T. kodakaraensis that were involved in the further degradation of GlcNAc2. Through a search of the T. kodakaraensis genome, one candidate gene identified as a putative β-glycosyl hydrolase was found in the near vicinity of the chitinase gene. The primary structure of the candidate protein was homologous to the β-galactosidases in family 35 of glycosyl hydrolases at the N-terminal region, whereas the central region was homologous to β-galactosidases in family 42. The purified protein from recombinant Escherichia coli clearly showed an exo-β-d-glucosaminidase (GlcNase) activity but not β-galactosidase activity. This GlcNase (GlmA Tk ), a homodimer of 90-kDa subunits, exhibited highest activity toward reduced chitobiose at pH 6.0 and 80°C and specifically cleaved the nonreducing terminal glycosidic bond of chitooligosaccharides. The GlcNase activity was also detected in T. kodakaraensis cells, and the expression of GlmA Tk was induced by GlcNAc2 and chitin, strongly suggesting that GlmA Tk is involved in chitin catabolism in T. kodakaraensis. These results suggest that T. kodakaraensis, unlike other organisms, possesses a novel chitinolytic pathway where GlcNAc2 from chitin is first deacetylated and successively hydrolyzed to glucosamine. This is the first report that reveals the primary structure of GlcNase not only from an archaeon but also from any organism.

scholarly journals Antibacterial Activity of Aqueous Extract of Momordica charantia and Terminalia catappa on Multidrug Resistant Invasive Escherichia coli isolated in Ready-to-Eat (RTE) Foods from Ekiti State

2019 ◽  
Vol 62 (3) ◽  
pp. 183-187
Author(s):  
Ajenifuja Oluwafemi Adeyemi ◽  
Ariyo David Oluwasegun
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Aqueous Extract ◽  
Multidrug Resistant ◽  
Momordica Charantia ◽  
Microbial Load ◽  
Dilution Technique ◽  
Smoked Fish ◽  
Terminalia Catappa ◽  
Agar Dilution

  Antibacterial activity of aqueous extract of Momordica charantia and Terminalia catappa was investigated against multidrug resistant invasive Escherichia coli isolated from ready-to-eat foods such as sausage rolls, meat pie, egg roll, doughnut and smoked fish. From the samples tested, smoked fish was found to have the highest microbial load of 1.05x106cfu/mL (55%) followed by meat pie 7.5x105cfu/mL (37%); Sausage roll 7.0x105cfu/mL (36%); and egg roll with 6.5x105cfu/mL (31%). Doughnut was found to have the lowest microbial load of 58x105cfu/mL (20%). The minimum inhibitory concentration (MIC) of the extract determined by agar dilution technique showed that M. charantia and T. catappa had an MIC of 0.5 g/mL and the degree of antibacterial potency of the extracts determined using broth dilution technique showed that at concentration of 0.5 mg/mL, M. charantia and T. catappa had diameter of zone of inhibition of 10.4mm and 11.2 mm, respectively. The observed antibacterial effect of the T. catappa was more pronounced. This investigation depicts positive results by the use of T. catappa extract in drug development for the treatment of infections caused by E. coli isolated from ready-to-eat foods.  

scholarly journals ANTI-DIARRHEAL ACTIVITY OF AQUEOUS EXTRACT OF NAGASARI FLOWERS (Mesua ferrea L.) IN BALB/c MICE INDUCED BY Escherichia coli

2020 ◽  
Vol 16 (2) ◽  
pp. 50-55
Author(s):  
Putu Monik Ananta Puspitarini ◽  
Iman Surya Pratama ◽  
Bambang Fajar Suryadi
Keyword(s):  
Escherichia Coli ◽  
Aqueous Extract
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