scholarly journals Antibacterial Activity of Aqueous Extract of Momordica charantia and Terminalia catappa on Multidrug Resistant Invasive Escherichia coli isolated in Ready-to-Eat (RTE) Foods from Ekiti State

2019 ◽  
Vol 62 (3) ◽  
pp. 183-187
Author(s):  
Ajenifuja Oluwafemi Adeyemi ◽  
Ariyo David Oluwasegun
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Aqueous Extract ◽  
Multidrug Resistant ◽  
Momordica Charantia ◽  
Microbial Load ◽  
Dilution Technique ◽  
Smoked Fish ◽  
Terminalia Catappa ◽  
Agar Dilution

  Antibacterial activity of aqueous extract of Momordica charantia and Terminalia catappa was investigated against multidrug resistant invasive Escherichia coli isolated from ready-to-eat foods such as sausage rolls, meat pie, egg roll, doughnut and smoked fish. From the samples tested, smoked fish was found to have the highest microbial load of 1.05x106cfu/mL (55%) followed by meat pie 7.5x105cfu/mL (37%); Sausage roll 7.0x105cfu/mL (36%); and egg roll with 6.5x105cfu/mL (31%). Doughnut was found to have the lowest microbial load of 58x105cfu/mL (20%). The minimum inhibitory concentration (MIC) of the extract determined by agar dilution technique showed that M. charantia and T. catappa had an MIC of 0.5 g/mL and the degree of antibacterial potency of the extracts determined using broth dilution technique showed that at concentration of 0.5 mg/mL, M. charantia and T. catappa had diameter of zone of inhibition of 10.4mm and 11.2 mm, respectively. The observed antibacterial effect of the T. catappa was more pronounced. This investigation depicts positive results by the use of T. catappa extract in drug development for the treatment of infections caused by E. coli isolated from ready-to-eat foods.  

scholarly journals Antibacterial Activities of Aqueous Extracts of Terminalia catappa, Momordica charantia and Acalypha wilkesiana on Escherichia coli Isolated from Pediatrics

2015 ◽  
Vol 58 (2) ◽  
pp. 72-76
Author(s):  
Ajenifuja Oluwafemi Adeyemi ◽  
Ajibade Victor Adeyinka ◽  
Fajilade Temilade Olawande
Keyword(s):  
Escherichia Coli ◽  
Inhibition Zone ◽  
Antibacterial Activities ◽  
Momordica Charantia ◽  
Dilution Technique ◽  
E Coli ◽  
Terminalia Catappa ◽  
Agar Dilution ◽  
Acalypha Wilkesiana ◽  
Broth Dilution

Antibacterial activity of aqueous extract of Terminalia catappa, Momordica charantia and  Acalypha wilkesiana was investigated against Escherichia coli isolated from pediatrics with the minimum inhibitory concentration (MIC) of 0.5mg/mL by agar dilution technique. The antibacterial potency of the extracts as evaluated by broth dilution technique, showed diameter of inhibition zone of 22.80 mm, 14.20 mm and 21.00 mm at a concentration of 0.5 mg/mL for T. catappa, M. charantia and A. wilkesiana, respectively. The antibacterial effect of T. catappa was found to be more pronounced with its plausible use for the treatment of infections caused by E. coli.

Phytochemical Content, Radical Scavenging and Antibacterial Properties of Aqueous Extract of Jatropha curcas Linn Leaves

2012 ◽  
Vol 1 (1) ◽  
Author(s):  
O B Oloyede
Keyword(s):  
Escherichia Coli ◽  
Hydrogen Peroxide ◽  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Aqueous Extract ◽  
Jatropha Curcas ◽  
Radical Scavenging ◽  
Klebsiella Pneumonia ◽  
Phytochemical Content ◽  
Proteus Species

This study investigated phytochemical content, radical scavenging and antibacterial activities of aqueous extract of leaves of Jatropha curcas Linn. Quantitative phytochemical analyses of alkaloids, phenols, tannins and flavonoids contents were carried out; radical scavenging activity was assessed using 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical and hydrogen peroxide inhibition assays while Disc diffusion and Agar well (ditch) diffusion methods were used for antibacterial activity against Klebsiella pneumonia, Escherichia coli, Pseudomonas aeruginosa, Staphilococcus aureus and Proteus species at 62.5, 125, 250 and 500 mg/mL. The extract contained alkaloids (1.600± 0.58 %), tannins (0.121 ± 0.00 mg/mL), phenols (0.463 ± 0.06 mg/mL) and flavonoids (0.672 ± 0.00 mg/mL), and showed radical scavenging activities against DPPH (IC50 = 21.24) and hydrogen peroxide (15.67 mg/mL) which were less than that of Butylated hydroxyanisole (BHA); IC50 = 3.92 (DPPH) and 6.19 mg/mL (hydrogen peroxide) respectively. It also showed antibacterial activity against Klebsiella pneumonia, Escherichia coli, and Pseudomonas aeruginosa at 250 and 500 mg/ml (MIC = 125 mg/mL), which were resistant to ampicillin, chloxacillin and erythromycin, and the extract was inactive against Staphilococcus aureus and Proteus species at these concentrations, though all were sensitive to gentamycin. This shows that the aqueous extract of leaves of Jatropha curcas Linn may possess some of the folkloric properties claimed.

scholarly journals Antibacterial Activity of Defatted and Nondefatted Methanolic Extracts of Aframomum melegueta K. Schum. against Multidrug-Resistant Bacteria of Clinical Importance

2020 ◽  
Vol 2020 ◽  
pp. 1-8
Author(s):  
Olufunmiso Olusola Olajuyigbe ◽  
Otunola Adedayo ◽  
Roger Murugas Coopoosamy
Keyword(s):  
Antibacterial Activity ◽  
Bacterial Infections ◽  
Methanol Extract ◽  
Clinical Importance ◽  
Multidrug Resistant ◽  
Resistant Bacteria ◽  
Plant Materials ◽  
Methanolic Extracts ◽  
Agar Dilution ◽  
Inhibition Zones

The antibacterial activity of the extracts of Aframomum melegueta including n-hexane extract (NHE), nondefatted methanol extract (NDME), and defatted methanol extract (DME) was investigated in this study. The NHE exhibited no antibacterial activity. The DME showed higher antibacterial activity than the NDME against the different isolates. At the highest concentration of 10 mg/mL in agar diffusion, NDME produced inhibition zones ranging from 11 to 29 mm against the microorganisms while DME produced inhibition zones ranging from 20 to 40 mm with the concentration of 10 mg/mL against the microorganisms. 0.1 mg/mL of the DME produced inhibition zones ranging between 12 and 14 mm in Aeromonas hydrophila ATCC 35654 and Pseudomonas aeruginosa ATCC 15442, respectively, while none of the isolates were inhibited by the NDME at a concentration of 1 mg/mL or less. In the agar dilution assay, the MICs of the NDME and DME ranged between 0.31 and 10 mg/mL, but more isolates were inhibited at 0.31 mg/mL of DME than those in NDME. In macrobroth assay, the MICs of the NDME ranged between 0.15 and 5.0 mg/mL and the MBCs ranged between 0.63 and 5.0 mg/mL, and the MICs of the DME ranged between 0.08 and 5.0 mg/mL and the MBCs were between 0.31 and 5.0 mg/mL. This study indicated that DME was more active with higher antibacterial activity than the NDME of this plant, and extracting the fatty portion of plant materials prior susceptibility testing would allow plant extracts to be more effective as well as justifying the use of Aframomum melegueta in traditional medicine for the treatment of bacterial infections.

scholarly journals Antibacterial Diterpenes from the Roots of Ceriops tagal

2008 ◽  
Vol 3 (1) ◽  
pp. 1934578X0800300 ◽  
Author(s):  
Musa Chacha ◽  
Renameditswe Mapitse ◽  
Anthony J. Afolayan ◽  
Runner R. T. Majinda
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Bacillus Cereus ◽  
Spectroscopic Data ◽  
Dilution Method ◽  
Agar Dilution Method ◽  
Bacterial Strains ◽  
Agar Dilution ◽  
Ceriops Tagal

Investigation of the roots of Ceriops tagal led to the isolation of a new isopimarane, together with the known diterpenes isopimar-8(14)-en-15,16-diol and erythroxyl-4(17),15(16)-dien-3-one. The structure of the new compound was identified as isopimar-8(14)-en-16-hydroxy-15-one. These structures were determined from extensive spectroscopic data analysis. The isolates were screened for antibacterial activity using the agar dilution method against ten test bacterial strains ( Bacillus cereus, Escherichia coli, Klebsiella pneumoniae, Micrococcus kristinae, Pseudomonas aeruginosa, Salmonella pooni, Serratia marcescens, Staphylococcus aureus, S. epidermidis and Streptococcus pyrogens). Isopimar-8(14)-en-16-hydroxy-15-one exhibited activity, with MIC values of 0.5 mg/mL against Streptococcus pyrogens; 0.25 mg/mL against Salmonella pooni and 0.1 mg/mL against Bacillus cereus, Staphylococcus aureus and Micrococcus kristinae.

scholarly journals Antibacterial Activity of Essential Oil of <i>Aeollanthus pubescens</i> on Multidrug Resistant Strains of <i>Salmonella</i> and <i>Escherichia coli</i> Isolated from Laying Hens Farming in Benin

2019 ◽  
Vol 09 (09) ◽  
pp. 804-823
Author(s):  
Aguidissou Oscar Nestor ◽  
Boko Kadoeito Cyrille ◽  
Sessou Philippe ◽  
Yovo Mahudro ◽  
Komagbe S. Gwladys ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Essential Oil ◽  
Laying Hens ◽  
Multidrug Resistant ◽  
Resistant Strains

scholarly journals Antibacterial Activity of Eight Medicinal Plants against Multidrug Resistant Escherichia coli and Salmonella spp. isolated from Broiler Meat

2020 ◽  
Vol 3 (4) ◽  
pp. 28-48
Author(s):  
Md. Rayhan Ali ◽  
Md. Omar Faruque ◽  
Md. Tarek Molla ◽  
Roksana Khanam ◽  
Shahin Mahmud ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Multidrug Resistant ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Zone Of Inhibition ◽  
Disc Diffusion Method ◽  
Salmonella Spp ◽  
Broiler Meat ◽  
Standard Disc

The excess use of antimicrobial agents in the poultry industry is a significant reason for the gradual spread and increasing level of multidrug resistance bacteria. This article is based on a study in which the antibacterial activity of aqueous, methanolic, ethanolic and acetonic extracts of eight medicinal plants were evaluated by standard disc diffusion method against multidrug resistant Escherichia coli and Salmonella spp. isolated from broiler meat. The multidrug resistance was checked by commercially available antibiotics using standard disc diffusion method. The results have indicated that the ethanolic extract of Boerhaavia diffusa showed maximum zone of inhibition against Escherichia coli, while Asparagus racemosus showed maximum zone of inhibition against Salmonella spp. Other experimental plant extracts had showed moderate activity against these multidrug-resistant bacteria, which can also be considered as potential source of active beneficial phytochemicals. Proper management and application of these plant extracts may be a wonderful alternative of commercially available antibiotic to minimize the risk.

scholarly journals Effect of Citrus aurantifolia Seed Extracts In Some Bacteria Isolated From Burns Infections

2014 ◽  
Vol 11 (2) ◽  
pp. 773-780
Author(s):  
Baghdad Science Journal
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Aqueous Extract ◽  
Antibiotic Sensitivity ◽  
Inhibition Zone ◽  
Acetone Extract ◽  
Hot Water ◽  
Alcoholic Extract

The present study was carried to evaluate antibacterial activity of Acetone, Alcholic (cold and hot) and Aqueaus(water) extracts of Citrus aurantifoliaseeds,against growth of some bacteria isolated from burns infections(Pseudomonas aeruginosa,Escherichia coli, Klebsiellapneumonia,Staphylococcusaureus)fromKindy HospitalIn Baghdad from March to June 2012.Antibiotic Sensitivity was done for all isolated bacteria used in this study.Results showed variation in antibacterial activity of different extracts against all tested bacteria by well diffusion technique in agar and measuring the diameter of inhibition zone, at concentration 250Mg-ml. Acetone extract had the greatest inhibitory effect followed by hot alcoholci extract, and then cold alcoholic extract,while the aqueous extract slightly inhibited bacteria. Minimum inhibitory concentration(MIC)were determined for all extracts against studying bacteria and found(12.5-50)mg-ml for acetone and alcoholic extracts, MIC for aqueous extract was 50mg-ml forPseudomonasaeruginosa and Escherichia coli,while was no effect onKlebsiellapneumonia and Staphylococcusaureus. Minimum Bacterial Concentration(MBC)were determined and was found25-50mg-ml for acetone extract,hot water was25mg-ml, cold alcoholic extract was 50mg-ml forPseudomonasaeruginosa,Escherichia coli andKlebsiella pneumonia but showed no effect on Staphylococcus aureus, aqueous extract showed negative effect on alltested bacteria. The antimicrobial activity of hot alcoholic extract of seeds was investigated practically (in vivo) by treating burns mices infected with tested bacteria(Pseudomonas aeruginosa,Escherichia coli and Klebsiellapneumonia),the results revealed good recovery at short time comparing with antibiotic(Flamazine) used at the same time.

scholarly journals ANTIMICROBIAL ACTIVITY OF METHANOL EXTRACT FROM STEM BARK OF Cinnamomum sintoc

2017 ◽  
Vol 17 (2) ◽  
pp. 77
Author(s):  
Nurdin Saidi ◽  
Hira Helwati ◽  
Lailatul Qhadariah Lubis ◽  
Muhammad Bahi
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Methanol Extract ◽  
Stem Bark ◽  
Dilution Method ◽  
Agar Dilution Method ◽  
E Coli ◽  
Paper Disk ◽  
Agar Dilution

Antimicrobial activity of methanol extract from stem bark of Cinnamomum sintoc has been evaluated against Candida albicans, Staphylococcus aureus and Escherichia coli. The extraction of compound was carried out by maceration, then isolation by column chromatograph, which yielded five (5) subfractions (A-E). Activity against fungus C. albicans, S. aureus bacteria dan E. coli using agar dilution method in paper disk. Methanol extract was not potent against antifungal activity but shows antibacterial activity with medium category. Subfraction C showed that antibacterial activity against S. aureus and E. coli with weak category, but subfractions D and E did not show any activity.

scholarly journals High prevalence of multidrug-resistant Escherichia coli isolates from children with and without diarrhoea and their susceptibility to the antibacterial activity of extracts/fractions of fruits native to Mexico

2017 ◽  
Vol 66 (7) ◽  
pp. 972-980 ◽  
Author(s):  
Magdalena de Jesús Uribe-Beltrán ◽  
Yesmi Patricia Ahumada-Santos ◽  
Sylvia Páz Díaz-Camacho ◽  
Carlos Alberto Eslava-Campos ◽  
Jesús Ernesto Reyes-Valenzuela ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Multidrug Resistant ◽  
High Prevalence
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