Antioxidant status of blood in patients with vibration disease

2019 ◽  
pp. 978-982 ◽  
Author(s):  
N. N. Malyutina ◽  
A. F. Bolotova ◽  
R. B. Eremeev ◽  
A. Zh. Gilmanov ◽  
D. Yu. Sosnin
Keyword(s):  
Antioxidant Activity ◽  
Blood Serum ◽  
Antioxidant Status ◽  
Total Antioxidant Status ◽  
Main Group ◽  
Biologically Active ◽  
Antioxidant Systems ◽  
Control Group ◽  
Vibration Disease ◽  
Total Antioxidant

Introduction. The overwhelming number of publications contains only data on the content of individual antioxidants, but not on the overall antioxidant activity of the blood in patients with vibration disease.The aim of the study was to determine the total antioxidant activity of blood serum in patients with vibration disease.Materials and methods. Th e main group consisted of 30 people diagnosed with “Vibration disease” of 1 degree (n=21) and 2 degrees (n=9). Th e control group consisted of 30 clinically healthy men, comparable in age with the main group (p=0.66). Th e total activity of antioxidant systems of blood plasma was evaluated photometrically using the test system “Total antioxidant status-Novo” (“Vector-best”, Russia).Results. The indicator of the total antioxidant status (TAS) was 1,038±0.232 mmol/l in the examined main group, against 1,456±0.225 mmol/l in the examined control group (p<0.000001). Th e coefficient of variation (CV) in patients with vibration disease was 22.35%, 1.45 times higher than in the control group (15.45%). In the main group there was a positive correlation between age and TAS (R=0.525), in the control group there was no such relationship (R=0.095). Th e degree of decrease depended on the severity of vibration disease.Conclusions. 1. The development of vibration disease is accompanied by a decrease in the antioxidant status of blood serum. 2. Th e degree of decrease in the antioxidant status of blood serum correlates with the severity of vibration disease. 3. Reduction of TAS can serve as a pathogenetic justification of the need to include drugs and/or biologically active additives with antioxidant activity in therapy

scholarly journals Dietary Plant Extracts Improve the Antioxidant Reserves in Weaned Piglets

Antioxidants ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 702
Author(s):  
Carlo Corino ◽  
Michel Prost ◽  
Barbara Pizzi ◽  
Raffaella Rossi
Keyword(s):  
Antioxidant Activity ◽  
Plant Extracts ◽  
Whole Blood ◽  
Antioxidant Status ◽  
Biological Activities ◽  
Total Antioxidant Status ◽  
Dietary Treatment ◽  
Control Group ◽  
Commercial Diet ◽  
Total Antioxidant

Reducing the use of antibiotics in livestock in order to contain antibiotic resistance and studying natural substance additives are key to sustainability. Among the various biological activities of plant extracts, antioxidant activity plays an important role. The present study assesses the total antioxidant activity and antioxidant reserves using the Kit Radicaux Libres test (KRL™ Kirial International, Couternon, France). One hundred and sixty piglets (Topics × Tempo) weaned at 28 days of age were divided into four dietary treatment groups that were fed a commercial diet (the control group, C); 500 mg/kg Boswellia extract (BOS); 200 and 50 mg/kg Uncaria and Tanacetum extracts (UT) respectively; and 225 mg/kg of an antioxidant plant extract mixture (AOX). The blood antioxidant activity of the piglets was measured using the KRL test and the reserves were analyzed on whole blood samples after hydrolysis with glucosidase, sulfatase and glucuronidase. No significant differences were observed in growth performance. The delta KRL values of the whole blood showed a significantly higher total antioxidant status of the piglets from the BOS and AOX groups than the UT and C groups (+30.7 BOS; +27.7 AOX vs. +17.81 UT +13.30 C; p = 0.002) between 18 and 28 days post-weaning. The delta KRL values of red blood cells (RBCs) showed a significantly higher total antioxidant status of the piglets from the AOX groups than the UT and BOS groups (+22.2 AOX; vs. +9.90 UT +9.4 BOS; p = 0.016) between the two sampling times. Reserves of UT and AOX were higher than C and BOS for all enzymes, glucosides, sulphates, and glucuronides. The biological KRL test proved to be an extremely sensitive tool to evaluate the piglets’ antioxidant status. Determining the antioxidant reserve also provides a better understanding of the real antioxidant status of pigs.

scholarly journals Relationship between Estradiol and Antioxidant Enzymes Activity of Ischemic Stroke

2009 ◽  
Vol 2009 ◽  
pp. 1-5 ◽  
Author(s):  
Nasrin Sheikh ◽  
Heidar Tavilani ◽  
Aliakbar Rezaie ◽  
Asad Vaisi-raygani ◽  
Saeedeh Salimi
Keyword(s):  
Antioxidant Activity ◽  
Superoxide Dismutase ◽  
Antioxidant Enzymes ◽  
Ischemic Stroke ◽  
Antioxidant Status ◽  
Total Antioxidant Status ◽  
Control Group ◽  
Significant Difference ◽  
Total Antioxidant ◽  
And Control

Some evidence suggests the neuroprotection of estrogen provided by the antioxidant activity of this compound. The main objective of this study was to determine the level of estradiol and its correlation with the activity of antioxidant enzymes, total antioxidant status and ferritin from ischemic stroke subjects. The study population consisted of 30 patients with acute ischemic stroke and 30 controls. There was no significant difference between estradiol in stroke and control group. The activity of superoxide dismutase and level of ferritin was higher in stroke compared with control group (P<.05,P<.001, resp.). There was no significant correlation between estradiol and glutathione peroxidase, glutathione reductase, catalase, total antioxidant status, and ferritin in stroke and control groups. We observed inverse correlation between estradiol with superoxide dismutase in males of stroke patients (r=−0.54,P=.029). Our results supported that endogenous estradiol of elderly men and women of stroke or control group has no antioxidant activity.

scholarly journals Serum Homocysteine and Reactive Species Levels in Fragility Fractures of the Pelvis

2019 ◽  
Vol 70 (9) ◽  
pp. 3216-3219 ◽  
Author(s):  
Alexandru Filip ◽  
Oana Viola Badulescu ◽  
Paul Dan Sirbu ◽  
Elena Cojocaru ◽  
Nina Filip ◽  
...  
Keyword(s):  
Bone Quality ◽  
Antioxidant Status ◽  
Total Antioxidant Status ◽  
Fragility Fractures ◽  
Reactive Species ◽  
Control Group ◽  
Serum Homocysteine ◽  
Total Antioxidant ◽  
The Relationship

Although there are studies showing the link between homocysteine and bone quality, the relationship remains controversial. The purpose of the study was to investigate homocysteine levels in patients with fragility fractures of the pelvis and their association with serum Total Antioxidant Status.The study included a group of 60 patients, aged ]65years, with pelvic fragility fractures and osteoporosis admitted in our trauma department between January 2015 and January 2018 and a control group of 30 patients, aged ]65years without fragility fractures or osteoporosis.The total antioxidant status (TAS) and homocysteine (Hcy) concentrations were determined in serum of all the patients. TAS levels had a significantly lower values in the group with pelvic fragility fractures compared with the control group (p[0.001).We noticed a significant higher mean of Hcy levels (p[0.001) in patients with pelvic fragility fractures compared with the control group. As conclusion this study shows that patients with fragility fractures of the pelvis have moderately elevated levels of homocysteine and reduced serum Total Antioxidant Status.

scholarly journals Rutin Content in Buckwheat Enriched Bread and Influence of its Consumption on Plasma Total Antioxidant Status

2009 ◽  
Vol 27 (Special Issue 1) ◽  
pp. S236-S240 ◽  
Author(s):  
T. Bojňanská ◽  
H. Frančáková ◽  
P. Chlebo ◽  
A. Vollmannová
Keyword(s):  
Antioxidant Status ◽  
Total Antioxidant Status ◽  
Biologically Active ◽  
Active Components ◽  
Overall Acceptability ◽  
Total Antioxidant ◽  
Acceptability Rating ◽  
Baked Products ◽  
Plasma Total Antioxidant Status

The goal of this study is the evaluation of enriched bread prepared with an addition of buckwheat as a source of biologically active components in nutrition. Experimental pastry was baked using buckwheat wholegrain flour blended with wheat flour in different portions (10%, 20%, 30%, 40% and 50%) in order to observe the influence of buckwheat addition on dough processing and the quality of the final baked products. Taking into account the overall acceptability rating, it was concluded that bread with an addition of up to 30% of buckwheat could be baked with satisfactory evaluation results. The content of rutin in loaves in a consumable form prepared with a buckwheat addition varied between 7.76 mg/kg and 26.90 mg/kg. The clinical study which followed the baking experiment was based on the consumption of enriched bread (30% buckwheat, between 34.7 mg/kg and 38.2 mg/kg rutin content in dry matter) by a group of volunteers during a period of four weeks. The results approved the increase of the total antioxidant status thanks to the buckwheat enriched bread consumption from the value of 1.135 ± 0.066 mmol/dm<sup>3</sup> at the beginning of the study to 1.46 ± 0.083 mmol/dm<sup>3</sup> at the end of the study, i.e. significant differences.

Oxidative stress increases in carbon monoxide poisoning patients

2010 ◽  
Vol 30 (2) ◽  
pp. 160-164 ◽  
Author(s):  
Havva Sahin Kavakli ◽  
Ozcan Erel ◽  
Orhan Delice ◽  
Gizem Gormez ◽  
Semra Isikoglu ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Carbon Monoxide ◽  
Antioxidant Status ◽  
Carbon Monoxide Poisoning ◽  
Total Antioxidant Status ◽  
Control Group ◽  
Total Oxidant Status ◽  
Oxidative Stress Parameters ◽  
Total Antioxidant ◽  
Oxidant Status

Objective: Carbon monoxide poisoning (COP) is one of the important causes of morbidity and mortality in toxicological cases. In this study, we aimed to find out more about the pathophysiology of COP by investigating the effects of COP on oxidative stress parameters such as total oxidant status (TOS) and total antioxidant status (TAS). Methods: Eighty-eight patients admitted to the emergency department of our hospital with acute COP and 35 healthy adults as control group were included in this study. Blood samples were collected from all COP patients at the time of initial emergency department evaluation to determine the oxidative stress parameters. Then, serum levels of total antioxidant status and total oxidant status levels were measured. Results: A total of 88 patients poisoned by carbon monoxide (CO; mean age 37.1 ± 18.2 years; 54’% women) were enrolled. TOS and carboxyhemoglobin (COHb) levels in COP patients were increased when compared to control group (p = 0.001). TOS, oxidative stress index (OSI) and COHb levels in COP patients were significantly lower after the treatment. (respectively, p = 0.016; p = 0.023; p = 0.001). On the other hand, no statistical differences were observed in TAS levels of study and control group as well as there were no changes with treatment. Conclusion: Measurements of TOS, TAS and OSI levels may be useful markers to find out the pathophsiology of COP.

scholarly journals Effects of Acute Administration of D,L-Homocysteine Thiolactone on the Antioxidative Status of Rat Intestine and Liver

2016 ◽  
Vol 66 (1) ◽  
pp. 26-36 ◽  
Author(s):  
Marija Stojanović ◽  
Ljiljana Šćepanović ◽  
Olivera Bosnić ◽  
Dušan Mitrović ◽  
Olga Jozanov-Stankov ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Status ◽  
Defense Mechanism ◽  
Total Antioxidant Status ◽  
Male Rats ◽  
Control Group ◽  
Acute Administration ◽  
Rat Intestine ◽  
Homocysteine Thiolactone ◽  
Total Antioxidant

AbstractOxidative stress appears to play a role in the pathogenesis of several inflammatory gastrointestinal diseases. Increased homocysteine levels may play a role in the pathogenesis of Chron’s disease and ulcerative colitis. The aim of this study was to examine the influence of homocysteine on the antioxidant status of rat intestine and liver. The levels of thiobarbituric acid reactive substances (TBARS), activity of catalase (CAT) and total antioxidant status (TAS) were investigated in the isolated gut and liver of young male rats in the control group (8 rats) and after 3-hоur incubation in high doses of D, L-homocysteine thionolactone (Hcy) (10 μmol/L) (8 rats). Samples of duodenum, ileum, colon and liver were homogenized in sodium phosphate buffer (1:10). Homogenates were centrifuged at 10000 for 10 min at 4° C and the supernatant was taken for biochemical assays. Our results showed that high D, L-homocysteine thionolactone concentration reduced enzymatic catalase activity in homogenates of the isolated segments of duodenum (27.04%) p<0.01; ileum (37.27%), colon (34.17%) and liver (67.46%) p<0.001. Exposition to high D,L-homocysteine thiolactone concentration significantly increased TBARS levels in the duodenum (106.05%), ileum (47.24%), colon (112.75%) and liver (32.07%) (p<0.01). Homocysteine also modifi ed the total antioxidant status of homogenates from the duodenum, ileum, colon and liver, increasing by 20.68% (duodenum), 24.74% (ileum), 14.88% (colon) and 19.35% (liver) (p<0.001). Homocysteine induced a consistent oxidative stress in rat’s intestine and liver (reduced activity of catalase and increased level of TBARS), but the elevated activity of TAS in our experiments could be explained as an adaptive response to the generated free radicals which indicates the failure of the total antioxidant defense mechanism to protect the tissues from damage caused by homocysteine.

scholarly journals Antioxidant system state in patients with multiple sclerosis current in therapy

2011 ◽  
Vol 57 (6) ◽  
pp. 661-670 ◽  
Author(s):  
L.P. Smirnova ◽  
N.V. Krotenko ◽  
E.V. Grishko ◽  
N.M. Krotenko ◽  
V.M. Alifirova ◽  
...  
Keyword(s):  
Multiple Sclerosis ◽  
Antioxidant Activity ◽  
Blood Serum ◽  
Antioxidant System ◽  
Malonic Dialdehyde ◽  
Control Group ◽  
Total Antioxidant Activity ◽  
Different Types ◽  
Total Antioxidant ◽  
High Level

Activity of erytrocyte glutationperoxidase (GP), glutationreductase (GR), glutationtransferase (GT), glucose-6-phosphatdehydrogenase (G6PDH), catalase and superoxiddysmutase (SOD), and also, the level of malonic dialdehyde (MDA) and total antioxidant activity of blood serum were studied in patients with different types of multiple sclerosis. Investigation of peripherical blood was carried out on first day of treatment and after standard therapy of copaxone. All MS patients had high level of MDA and activity of GP in erythrocytes in comparison with a control group. Other antioxidant enzymes of erythrocytes and total antioxidant activity of blood serum exhibited weak positive dynamics in patients with a relapsing remittance of multiple sclerosis (RRMS). Decrease of activity of antioxidant system in patients with secondary progression multiple sclerosis (SPMS) was more pronounced and remained unchanged after the treatment. This is consistent with the more severe clinical course of thise disease.

Effect of Piperine on the Pharmacokinetics and Pharmacodynamics of Glimepiride in Normal and Streptozotocin - Induced Diabetic Rats

2012 ◽  
Vol 7 (10) ◽  
pp. 1934578X1200701 ◽  
Author(s):  
Ciddi Veeresham ◽  
Samala Sujatha ◽  
Thatipamula Sandya Rani
Keyword(s):  
Biochemical Parameters ◽  
Antioxidant Status ◽  
Total Antioxidant Status ◽  
Diabetic Rats ◽  
Pharmacokinetic Parameters ◽  
Control Group ◽  
Diabetic Patients ◽  
Significant Protection ◽  
Pharmacokinetics And Pharmacodynamics ◽  
Total Antioxidant

The effect of piperine on the pharmacokinetics and pharmacodynamics of glimepiride in normal as well as diabetic rats was studied. In normal and streptozotocin induced diabetic rats the combination of glimepiride with piperine increased all the pharmacokinetic parameters, such as Cmax, AUC0-n, AUCtotal, t½, and MRT, and decreased the clearance, Vd, markedly as compared with the control group. In pharmacodynamic studies, the combination of glimepiride with piperine provided significant protection against the diabetes induced alterations in the biochemical parameters. In addition, the combination of glimepiride with piperine also improved the total antioxidant status significantly in diabetic rats compared with piperine and glimepiride treated groups. The results revealed that a combination of glimepiride with piperine led to the enhancement of the bioavailability of glimepiride by inhibiting the CYP2C9 enzyme, which suggested that piperine might be beneficial as an adjuvant to glimepiride in a proper dose, in diabetic patients.

scholarly journals Assessment of Lipid Peroxidation Levels and Total Antioxidant Status in Chronic and Aggressive Periodontitis Patients: An in vivo Study

2018 ◽  
Vol 19 (3) ◽  
pp. 287-291 ◽  
Author(s):  
Ashish Verma ◽  
Vivek Tripathi ◽  
Sahib T Singh ◽  
Chetan D Singh ◽  
Jaspreet S Gill
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Antioxidant Status ◽  
Total Antioxidant Status ◽  
Study Groups ◽  
Aggressive Periodontitis ◽  
Stress Index ◽  
Control Group ◽  
Total Antioxidant

ABSTRACT Introduction Periodontitis is a common problem affecting a significant population of the world. For the assessment of oxidative stress of an individual, total oxidation status (TOS) and total antioxidant capacity (TAOC) are the significant biomarkers. Hence, we planned the present study to assess malondialdehyde (MDA), TOS, TAOC levels, and oxidative stress index (OSI) in generalized aggressive periodontitis (GP) and chronic periodontitis (CP) patients. Materials and methods The present study included assessment of 40 CP patients, 40 GP patients, and 40 healthy controls. Clinical assessment of all the subjects was done by measuring the probing depth (PD), clinical attachment (CL), gingival index (GI), gingival bleeding index (GBI), and plaque index (PI). Salivary and serum samples were taken and assessed by standard procedures as described previously in the literature. All the values were assessed and compared. Results Significant results were obtained while comparing all the periodontal parameters in between various study groups. Mean serum MDA levels in the CP, GP, and control group were found to be 0.68, 0.65, and 0.61 µM respectively. Statistically nonsignificant results were obtained while comparing the serum MDA levels in between the three study groups. Significant results were obtained while comparing the mean serum and salivary TOS values, TAOC values, and OSI in between various study groups. Conclusion In periodontitis patients, oxidative stress was significantly higher in comparison with healthy subjects. Clinical significance Oxidative parameters do play a significant role in the pathologic profile of periodontitis. How to cite this article Tripathi V, Singh ST, Sharma V, Verma A, Singh CD, Gill JS. Assessment of Lipid Peroxidation Levels and Total Antioxidant Status in Chronic and Aggressive Periodontitis Patients: An in vivo Study. J Contemp Dent Pract 2018;19(3):287-291.

Serum malondialdehyde level and activity of total antioxidant status of dogs with age-related cataract

2016 ◽  
Vol 19 (2) ◽  
pp. 429-431 ◽  
Author(s):  
J. Madany
Keyword(s):  
Antioxidant Status ◽  
Colorimetric Method ◽  
Total Antioxidant Status ◽  
Thiobarbituric Acid ◽  
Control Group ◽  
Study Objective ◽  
Age Related ◽  
Significant Difference ◽  
Total Antioxidant ◽  
Cataract Development

Abstract Study objective: determination of malondialdehyde (MDA) level and total antioxidant status (TAS) in the serum of dogs with age-related cataract. Material and methods: Forty dogs, 10-16 years of age, which were diagnosed with age-related cataract were examined. The control group consisted of 12 dogs, 8-13 years of age, without cataract. MDA was determined using a Perkin-Elmer LS 30 luminescence spectrometer using the method with thiobarbituric acid (TBA). TAS was determined using the colorimetric method with a set of Randox Total Antioxidant Status reagents. Results: The results show a higher level of MDA with statistically significant difference and lower activity of TAS in the serum of dogs with age-related cataract. Conclusions: The results obtained indicate higher systemic lipid peroxidation and weakening of the general defense system in dogs with age-related cataract. These results suggest that such conditions may have an effect on the development of age-related cataract and are consistent with the theory of free radical age-related cataract development.

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