scholarly journals THE COMBINATION OF MURASHIGE AND SKOOG (MS) MEDIA AND ACTIVATED CHARCOAL ON THE GROWTH OF THE Vanda helvola ORCHID PLANT IN VITRO

2021 ◽  
Vol 3 (1) ◽  
Author(s):  
Ata Rofita Wasiati ◽  
Ika Afifah Nugraheni ◽  
Yuli Setiawati
Keyword(s):  
Tissue Culture ◽  
Activated Charcoal ◽  
Ornamental Plants ◽  
Negative Control ◽  
Culture Techniques ◽  
Plant Seeds ◽  
Randomized Design ◽  
Short Period ◽  
The Cost

Orchids of the Vanda helvola genus are extensively grown as ornamental plants. The method of propagation of plant seeds in tissue culture is an alternative that may be employed in the provision of a short period. Murashige and Skoog (MS) media and activated charcoal can optimize orchid cultivation utilizing tissue culture techniques. One of the keys to success in tissue culture is the application of natural types of growing regulatory substances (ZPT) at the proper concentration, which can lower the cost of orchid tissue culture.The objective of this study was to see how a combination of Murashige and Skoog (MS) media and activated charcoal influenced the Vanda helvola orchid subculture in vitro. The researchers employed a Complete Randomized Design (RAL) with four treatments and three repeats: negative control (K), ¼ MS (K1), ½ MS (K2), and 100% MS (K3) (K3). The observed parameters include the percentage of live explant, number of roots, and height of orchid plants.   The results showed that the explant had a 100% life percentage of over 21 HST and had no noticeable influence on the number of roots and the plant's height.

scholarly journals STUDI PENGARUH MEDIA ALTERNATIF UNTUK PERBANYAKAN PISANG BARANGAN (Musa acuminata L.) SECARA IN-VITRO

2021 ◽  
Vol 12 (1) ◽  
pp. 33
Author(s):  
Rosmaina Rosmaina ◽  
Ragil Endika ◽  
Zulfahmi Zulfahmi
Keyword(s):  
Tissue Culture ◽  
Culture Media ◽  
Low Cost ◽  
Ms Medium ◽  
Liquid Fertilizer ◽  
Culture Techniques ◽  
Randomized Design ◽  
Media Component ◽  
The Cost

The main obstacles in the commercialization of seedlings mass propagation through tissue culture techniques is the high cost of the culture media component. therefore, the production of lowcost tissue culture is required. This study aims to develop low cost in-vitro media for the production of the seedlings of Barangan banana. The research was composed following Factorial Completely Randomized Design, the first factor was Terra Novalgro liquid fertilizer with three concentrations, namely 1, 2, and 3 ml L-1, while the second factor was Gandasil with 3 concentrations namely 1, 2, and 3 mg L-1, so obtained nine treatments, each treatment was repeated 10 times, so that there were 90 experimental units. MS media was used as control. The parameter observed was number of shoots, number of leaves and number of roots. The results of this study exhibited that the treatment of 1 ml L-1 liquid fertilizer + 2 mg L-1 foliar fertilizer produced 9.30 shoots/explant and 1.90 leaves/explant, that no significantly different from MS medium (control) which produced 9.0 shoots/explant and 0.3 leaves/explants. Therefore, the using completed liquid fertilizers and foliar fertilizers as medium in vitro propagation of barangan bananas can become an alternative replace MS medium, as well as reduced the cost of culture media as 91% -93% compared to MS media.

scholarly journals Multiplikasi Eksplan Tunas Anggrek Hitam (Coelogyne pandurata Lindl.) dengan Penambahan NAA (Naphthalene Acetic Acid) dan Ekstrak Biji Jagung (Zea mays) Secara In Vitro

2021 ◽  
Vol 11 (2) ◽  
pp. 114
Author(s):  
Nurkapita Nurkapita ◽  
Riza Linda ◽  
Zulfa Zakiah
Keyword(s):  
Tissue Culture ◽  
Zea Mays ◽  
Acetic Acid ◽  
Seed Extract ◽  
Naphthalene Acetic Acid ◽  
Shoot Height ◽  
Culture Techniques ◽  
Corn Seed ◽  
Randomized Design

(Article History: Received February 18, 2021; Revised April 27, 2021; Accepted May 19, 2021) ABSTRAKPerkembangbiakan anggrek secara generatif alami membutuhkan bantuan jamur mikoriza untuk perkecambahan biji, sedangkan usaha perbanyakan konvensional memerlukan waktu lama untuk memperoleh tanaman dalam jumlah banyak. Salah satu alternatif untuk perbanyakan anggrek hitam (Coelogyne pandurata Lindl.) adalah melalui multiplikasi tunas anggrek secara in vitro. Tujuan penelitian adalah untuk membuktikan pengaruh pemberian NAA (Naphthalene Acetic Acid) dan ekstrak biji jagung (Zea mays) terhadap multiplikasi tunas anggrek hitam. Penelitian dilakukan di Laboratorium Kultur Jaringan Jurusan Biologi Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Tanjungpura Pontianak. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) pola faktorial dengan dua faktor perlakuan. Faktor pertama adalah NAA terdiri dari 5 taraf konsentrasi yaitu A0 (0 M/ kontrol) A1 (10-7 M), A2 (10-6 M), A3 (5x10-7 M) dan A4 (5x10-6 M ) dan faktor ekstrak biji jagung (B) dengan 5 taraf konsentrasi yaitu B0 (0%), B1 (2,5%), B2 (5%); B3 (7,5%) dan B4 (10%). Pemberian kombinasi NAA dan ekstrak biji jagung berpengaruh nyata terhadap semua parameter yaitu jumlah tunas, jumlah daun, dan tinggi tunas. Hasil terbaik rerata jumlah tunas pada perlakuan A4+B4 yaitu 5x10-6M NAA+10% ekstrak biji jagung. Hasil terbaik pada rerata jumlah daun pada perlakuan A2+B2 yaitu 5x10-7M NAA+5% ekstrak biji jagung dan hasil terbaik pada rerata tinggi tunas pada perlakuan A1+B1 yaitu 10-7M NAA+2,5% ekstrak biji jagung.Kata Kunci: multiplikasi; tunas anggrek hitam; ekstrak biji jagung; NAA. ABSTRACTGenerative reproduction of orchid plants it takes a requires the help of mycorriza mushrooms for seed germination, whereas conventional propagation business takes a long time to obtain large quantities of plants. One alternative to the propagation black orchids (Coelogyne pandurata Lindl.) is required through tissue culture techniques. The purpose of this study is to find the influence and concentration corn seed extract (Zea mays) and NAA (Naphthalene Acetic Acid) on the multiplication black orchids. This research was conducted in the tissue culture laboratory Biology Department Faculty of Mathematics and Natural Sciences Tanjungpura University Pontianak. The study used a Complete Randomized Design (RAL) of factorial patterns with two treatment factors. The first factor is that the NAA consists of 5 concentration levels  A0 (0 M) A1 (10-7 M), A2 (10-6 M), A3 (5x10-7 M) and A4 (5x10-6 M ) and the second factor is that corn seed extract of 5 levels concentratio B0(0%), B1 (2,5%), B2 (5%); B3 (7,5%) and B4 (10%). The administration NAA and corn seed extract in combination has a real effect on all parameters namely the number shoots, the number leaves, and the height shoots. The best results where the average number of shoots in the treatment of A2+B2 namely 5x10-6M NAA + 10% corn seed extract. The best results average number of leaves in the treatment  A2+B2 namely 5x10-7M NAA + 5% corn seed extract and in the best results for shoot height in the treatment of A1+B1 namely 10-7M NAA + 2.5% corn seed extract.Keywords: Multiplication; black orchid’s shoot; corn  seed extract; NAA

scholarly journals Study of Seaweed Kappaphycus alvarezii Explants Growth in the Different Salinity Concentrations

2021 ◽  
Vol 13 (1) ◽  
pp. 97
Author(s):  
Muhammad Aris ◽  
Fatma Muchdar ◽  
Rusmawati Labenua
Keyword(s):  
Tissue Culture ◽  
Kappaphycus Alvarezii ◽  
Environmental Parameters ◽  
Culture Method ◽  
Salinity Treatment ◽  
Culture Methods ◽  
Culture Techniques ◽  
Randomized Design ◽  
Salinity Levels

HighlightThe best salinity for the thallus growth of K. alvarezii is 32 mg/LThe salinity indicates the osmotic balance of K. alvarezii seaweedThis osmoregulation process affects the nutrient absorption of K. alvarezii seaweedThis study aims to develop the availibility of superior seeds of K. alvareziiAbstract Kappaphycus alvarezii is one of the leading commodities in Indonesian waters. Demand for this commodity is quite high, as reflected in the increasing volume of exports each year. Fulfillment of these demands is obtained from the production of cultivation. Generally farmers get natural seaweed seedlings, namely cuttings from existing seaweed. The continuous use of seeds from nature can cause deterioration in the quality and quantity. Handling the problem of quality deterioration from seaweed seeds originating from nature, can be overcome by multiplying the seeds through tissue culture methods in vitro. In term of tissue culture techniques method, the most important thing to note is environmental parameters. An environment parameter that changes suddenly such as salinity can inhibit the growth of seaweed. Thus, this work is attampting the different salinity treatment on seaweed explants K. alvarezii. This study aims at determining (weight) the explants of K. alvarezii with a comparison of the different salinity levels in the in vitro tissue culture method. The method used in this study was a completely randomized design (CRD) with the different salinity treatments namely 30, 31, 32, 33, and 34 ppt. The results showed that the different salinities influenced the growth rate of K. alvarezii seaweed explants with the best explant growth at the salinity of 31 ppt, while the lowest growth value was obtained at 34 ppt

scholarly journals Optimasi Konsentrasi Kinetin dan Benzyl Amino Purine Pada Kultur Tunas Vanili (Vanilla planifolia)

2021 ◽  
Vol 21 (1) ◽  
pp. 54-57
Author(s):  
Dyah Nuning Erawati ◽  
Yusriatul Mawaddah ◽  
Siti Humaida ◽  
Irma Wardati
Keyword(s):  
Tissue Culture ◽  
Shoot Multiplication ◽  
Culture Techniques ◽  
Planting Material ◽  
Randomized Design ◽  
Wet Weight ◽  
The Mean ◽  
Completely Randomized Design ◽  
Tissue Culture Techniques

Vanilla has a potential to be developed through tissue culture techniques to anticipate the limitations of the parent plant as a source of planting material. The in vitro propagation ability of vanilla shoots needs to be controlled with the regulation of Kinetin and Benzyl Amino Purines. The interests of this study are 1) analysis of the response of vanilla explants at several Kinetin concentrations; 2) analysis of the response of vanilla explants at several concentrations of BAP and 3) analysis of the interaction of Kinetin and BAP on the response of vanilla explants to form shoot multiplication. The research was conducted at the Tissue Culture Laboratory Politeknik Negeri Jember from June to December 2020 using a factorial Completely Randomized Design (CRD). Factor 1 was the Kinetin concentration of 0.0, 1.0, 2.0 mg.L-1 and the second factor was the concentration of BAP 0.5, 1.5, 2.5 mg.L-1. The results proved that the fastest shoot multiplication occurred on MS medium + Kinetin 2 mg.L-1 with a mean of 8.7 days after inoculation. The mean number of shoots was 7.6 shoots/explant with the highest average wet weight of 0.9 grams/explant at the addition of BAP 1.5 mg. L-1 at measurement 70 days after inoculation.

scholarly journals Pengaruh Kombinasi Sitokinin dan Gibberelin terhadap Pemanjangan Tunas Jambu Mete (Anacardium Occidentale L.) secara In Vitro

Jurnal BIOMA ◽  
2014 ◽  
Vol 10 (1) ◽  
pp. 34
Author(s):  
Febrina Ariyanti ◽  
Christiani Tumilisar ◽  
Rossa Yunita
Keyword(s):  
Tissue Culture ◽  
Economic Value ◽  
Shoot Elongation ◽  
Anacardium Occidentale ◽  
Culture Techniques ◽  
Randomized Design ◽  
Alternative Techniques ◽  
Completely Randomized Design ◽  
Tissue Culture Techniques

Abstract Cashew (Anacardium occidentale L.) is a plant with high economic value. Conventional propagation of this plant still has obstacles, so an alternative techniques using tissue culture could be tried. One of the factors that determine the success of tissue culture techniques is the type and concentration of growth regulators was used. Growth regulator which have effect on shoot elongation is a cytokinin and gibberellin, this research tried to investigate the influence of combination cytokinin and gibberelin on in vitro shoot elongation of cashew. This research was conducted at BB-Biogen, Bogor on June-November 2010. The method in this research was to design experimental method with completely randomized design. The result was cytokinin could increase the length of shoots and quantity of shoots very well until 4 cm and quantity of shoot for 5. With the most effective cytokinin is zeatin of 5 mg/l.   Key words: Anacardium occidentale L., cytokinin, elongation of shoots, gibberelin

scholarly journals Plant Tissue Culture- A New Tool for Vegetable Improvement (Indian scenario): A Review

2021 ◽  
Author(s):  
Priyanka Bijalwan ◽  
Shilpa .
Keyword(s):  
Tissue Culture ◽  
Embryo Culture ◽  
Plant Tissue Culture ◽  
Plant Tissue ◽  
Plant Cells ◽  
Meristem Culture ◽  
Culture Techniques ◽  
Short Period ◽  
Plant Sciences

In vitro culture of plant cells/tissues is now routine using a range of explant types from many of the important vegetable and fruit crops. Successful technologies include isolation, culture of tissues, cells, protoplasts, organs, embryos, ovules, anthers and microspores and regeneration from them of complete plantlets. The development of plant tissue culture technology represents one of the most exciting advances in plant sciences. For example, the prospect of being able to introduce, develop, produce, transfer and conserve the existing gene pool of plant sciences by using tissue culture methods opens up new opportunities for researches and entrepreneurs. The term plant tissue culture should denote in vitro cultivation of plant cells or tissues in an unorganized mass, i.e., callus culture. Plant tissue culture techniques, in combination with recombinant DNA technology, are the essential requirements for the development of transgenic plants. However, culture techniques like anther/pollen/ovule culture, meristem culture can themselves be utilized for crop improvement or may serve as an aid to conventional breeding. In recent, isolated microspore culture has developed as a breeding tool and an experimental system for various genetic manipulations. The inherent potentiality of a plant cell to give rise to a whole plant, a capacity which is often retained even after a cell has undergone final differentiation in the plant body, is described as ‘cellular totipotency’. On the other hand, production of virus-free plants via meristem culture can reduce losses caused by phyto-pathogens. Embryo culture has many potential uses ranging from overcoming seed dormancy to facilitation of inter-specific hybridization. Protoplast fusion technique can be used for the transfer of cytoplasmic male sterility from one species to another in a short period of time. In cabbage, male sterile cybrids are being utilized by seed companies to produce hybrid seeds on commercial scale and at competitive rates. Plant tissue culture and cell culture are providing useful methods for germplasm storage either by low temperature storage of organized tissue, or cryopreservation of cell or embryo culture.

scholarly journals IN VITRO MULTIPLICATION OF JABON (ANTHOCEPHALUS CADAMBA (ROXB)) ON VARIOUS CONCENTRATION OF BAP AND IAA

2018 ◽  
Vol 4 (2) ◽  
pp. 60
Author(s):  
Asgar Taiyeb ◽  
Baharuddin Baharuddi
Keyword(s):  
Tissue Culture ◽  
Culture Techniques ◽  
In Vitro Multiplication ◽  
Supportive Environment ◽  
Randomized Design ◽  
The Media ◽  
Completely Randomized Design ◽  
The Difference ◽  
Tissue Culture Techniques

One of problems in the Jabon propagation is the availability of seeds both quality and quantity. Tissue culture technology is one of the alternatives that can be used for the supply of  Jabonseeds to produce organs of plants (buds, leaves, roots). The success of plant tissue culture techniques is determined by the condition of explants, a supportive environment and the addition of growth regulators are expected to provide a response to the cultured explants. This study aimed to know the effect of Benzyl Amino Purine (BAP) and Indole Acetic Acid (IAA) to  in vitro multiplication of Jabon. This research conducted at the Laboratory of Forestry Sciences, Faculty of Forestry, University of Tadulako from March to May 2015. Using a completely randomized design with treatments: 0.1 mg / l IAA + 1 mg / l BAP (JB1), 0.1 mg / l IAA + 1.5 mg / l BAP (JB2), 0.1 mg / l IAA + 2 mg / l BAP ( JB3) and 0.1 mg / l IAA + 2.5 mg / l BAP (JB4). Each treatment was repeated three times to obtain 12 experimental units. The results showed that the difference in response Jabon of treatment tested was the highest number of buds and leaves were in the media added 0.1 mg / l IAA + 1.5 mg / l BAP. Furthermore, the formation of callus obtained in media which added 0.1 mg / l IAA + 1 mg / l BAP.

scholarly journals Pertumbuhan Tunas Pisang Barangan (Musa Acuminata Colla.) akibat Pemberian Benzyl Amino Purin dan Arang Aktif secara In Vitro.

2019 ◽  
Vol 4 (1) ◽  
pp. 73-90
Author(s):  
Mulia Saputri ◽  
Marai Rahmawati ◽  
Elly Kesumawati
Keyword(s):  
Tissue Culture ◽  
Activated Charcoal ◽  
Shoot Growth ◽  
Growth Time ◽  
Shoot Height ◽  
High Productivity ◽  
Horticultural Crops ◽  
Randomized Design ◽  
And Control

Pertumbuhan Tunas Pisang Barangan Akibat Pemberian Benzyl AminoPurin dan Arang Aktif secara In Vitro Accretion of Barangan Banana Shoot Effect of  (BAP)  and Activated Charcoal Explant by In VitroAbstrak. Banana as a superior product of horticultural crops, has not achieved high productivity and has several obstacles in its multiplication. Tissue culture is one solution to overcome this problem. This study aims to determine the composition of PGRBenzil Amino Purine (BAP) and activated charcoal that are appropriate in the multiplication of barangan banana shoots. This research was conducted at the Tissue Culture Laboratory Faculty of Agriculture Syiah Kuala University, Darussalam Banda Aceh. The design used was a Completely Randomized Design (CRD) with two treatment factors. The first factor is BAP concentration consisting of 3 levels, namely 4 mg/L, 6 mg /L, and 8 mg/L. The second factor is activated charcoal concentration consisting of 3 levels, namely control, 1 g/L and 2 g/L. In this study, from 9 treatment combinations, only 4 treatment combinations were not contaminated. Of the 4 treatment combinations the combination of BAP concentration of 6 mg/L and control (without activated charcoal) showed the fastest shoot growth time of 29 days after multiplication, the most shoot growth was 6 shoots and the average shoot height was 15.9 mm

scholarly journals PENGARUH ARANG AKTIF, BENZILADENIN, DAN KINETIN TERHADAP PERTUMBUHAN TUNAS JATI SOLOMON (Tectona grandis Linn. f) IN VITRO

2019 ◽  
Vol 5 (2) ◽  
pp. 21-30
Author(s):  
Husen Hariadi ◽  
Yusnita Yusnita ◽  
Melya Riniarti ◽  
Dwi Hapsoro
Keyword(s):  
Growth Regulator ◽  
Activated Charcoal ◽  
Experimental Treatment ◽  
Tectona Grandis ◽  
Ms Medium ◽  
Culture Techniques ◽  
Shoot Bud ◽  
Randomized Design ◽  
Number Of Leaves

Tissue culture techniques can be used for propagation of uniformaly large teak solomon seeds.The purpose of this research was knowing the effect of activated charcoal, the addition of benzyladenine (BA) and combination of BA with 6-furfurylaminopurine (kinetin) to the growth of shoots of solomon teak in vitro. The solomon teak explants used were singlestem cuttings from aseptic shoots obtained from in vitro cultures. This research was conducted in laboratory with complete randomized design with 3 replications. The experimental treatment was a single factor consisting of basic MS medium (Murashige and Skoog, 1962), with 6 treatments: MS without growth regulator (control), MS without growth regulator + 2 g/l activated charcoal, MS + 0,1 m/l BA , MS + 0,2 m/l BA, MS + 0,1 m/l BA + 0,1 m/l kinetin and MS + 0,2 m/l BA + 0,1 m/l kinetin. Observation on the number of books/ shoots, number of leaves/ shoots, shoot/ bud height and visual apperance of culture was taken at 8 weeks after planting. The data were analyzed for variety and continue the separation of the LSD at 5% level. The results showed that in general, all six treatments could be used for propagation of in vitro teak solomon (Tectona grandis Linn. f) and produced at least 6,22 books/ shoots every 8 weeks. The best media were MS medium + 0,1 m/l BA and MS + 0,1 m/1 BA + 0,1 m/l kinetin, because it able to produce 7,78 books/ shoots. The highest number of leaves was obtained at the treatment of MS + 0,1 m/l BA, while the average shoots/ shoots produced were not different for all.

scholarly journals PROPAGASI IN VITRO ANGGREK (Dendrobium phalaenopsis Fitzg) TERHADAP PEMBERIAN HORMON IBA DAN KINETIN

2017 ◽  
Vol 7 (1) ◽  
pp. 15
Author(s):  
IMAM MAHADI
Keyword(s):  
Tissue Culture ◽  
Plant Growth ◽  
Growth Regulator ◽  
Plant Growth Regulator ◽  
Ornamental Plants ◽  
Special Treatment ◽  
Culture Techniques ◽  
Tissue Culture Techniques ◽  
Number Of Shoots

Orchids are a very popular ornamental plants, but orchids have a very slow vegetative growth, thus requiring special treatment in the grown.To  spur grow than development can be used through tissue culture techniques of multiplication. But the success of the technique of tissue culture is inseparable with give a substance on the plant growth regulator. This study aims to determine the growth  an influence  D.phalaenopsis Fitzg orchids of giving a gaint swith IBA administration and Kinetin according to in vitro  and as well as to obtain a concentration combination of  IBA and Kinetin on the best orchid D.Phalaenops is Fitzg explants.  Base don’t he results of the study indicate that the combination of IBA and Kinetin concentration giving a influence percentage of live explants,   number of shoots and number of roots orchid D. phalaenopsis Fitzg explants.Combination treatment of IBA 1.0 ppm and 0.1ppm Kinetin is a combination of the best treatment to produce a number of shoots orchids.The giving of IBA 0.5ppm in the medium without the addition of kinetin concentration can increase the number of roots of the orchid D.phalaenopsis Fitzg.

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