scholarly journals SELECTION OF AN EXTRACTANT FOR ISOLATION OF BIOLOGICALLY ACTIVE COMPOUNDS FROM A HEDYSARUM NEGLECTUM

2020 ◽  
Author(s):  
Anastasia Dmitrieva ◽  
Dyshlyuk Lubov ◽  
Fotina Natalia

Global technological progress is one of the main problems of ecosystem deterioration. The high concentration of industrial enterprises contributes to the development of the region's environmental crisis and the decline in public health. An increase in the environmental pollutant contributes to the activation of oxidative stress in the human body, which plays a key role in the pathogenesis of a number of chronic diseases (cancer, diabetes, cardiovascular diseases, etc.), leading to a decrease in life expectancy and early aging of the body. To increase the adaptive abilities of the human body, it is important to use plant raw materials. A promising source of natural nutrients is the Hedysarum neglectum Ledeb. In order to obtain the extract of the forgotten kopek, the method of extraction was used at room temperature with a reverse refrigerator for 2 hours. In this study, to determine the working parameters of extraction (type of extractant, concentration of extractant), a selection of various extractive substances was selected. The efficiency of the extraction process was determined by two parameters: extractivity and yield of biologically active substances. The maximum extractivity was observed when using 50 % ethyl alcohol. To determine the working concentration of the water-ethanol mixture, the prepared extracts were analyzed for the content of flavonoids, tannins, and ascorbic acid. The maximum yield of biologically active substances (flavonoids 419.02±3.58 mg%, tannins 8.10±1.03 %, ascorbic acid 691.04±3.62 mg/kg) was observed during extraction with 50% water-ethanol mixture. The obtained results suggest that the water-ethanol extract of Hedysarum neglectum Ledeb. it is a potential source of natural biologically active substances for creating functional food products.

2020 ◽  
pp. 66-72
Author(s):  
Viktoriya Pogarskaya ◽  
Raisa Pavlyuk ◽  
Tatyana Kotuyk ◽  
Olga Yurieva ◽  
Nadiya Maksymova

The aim of research is to develop a new method for the production of protein plant nanoadditives from dry chickpea in the form of nanopowders and nanopastes using the processes of steam thermo-mechanical destruction, leading to a high degree of mechanical destruction of biopolymers in separate constituent monomers, without destroying the latter. The new method is based on the use as an innovation of a complex effect on raw materials of steam-thermal treatment in modern steam-thermal devices (at a temperature of +70 °C) and non-enzymatic catalysis, which occurs when using fine grinding. The proposed method makes it possible to more fully reveal the biological potential of plant raw materials and transform hardly soluble, indigestible by the human body components of raw materials (in particular, cellulose, pectin substances, proteins and their nanocomplex and nanoassociates) into components that are easily absorbed by the body. The method makes it possible to more completely remove from the raw materials inactive bound forms of individual monomers of biopolymers, to reduce the molecular weight of biopolymers. At the same time, the efficiency of the resulting product increases significantly when consumed by the human body. New additives from chickpea are fundamentally different from traditional ones in properties and physicochemical state. The developed additives act simultaneously five in one: a source of essential amino acids and other biologically active substances, structure formers, gelling agents, thickeners and yellow dyes. The obtained protein supplements from chickpeas in combination with herbal Nanoadditives (from pumpkin, carrots, lemons, garlic, celery roots, ginger) were used as recipe components in the development of a new generation of confectionery products. The resulting confectionery products differ from the traditional ones in their low sugar and fat content (up to 5 %), high content of complete protein (from 13 to 20 %). In addition, 100 g of new products are able to satisfy the daily requirement for biologically active substances (β-carotene, phenolic compounds) and 0.5 daily requirement for vitamin C. The resulting products are natural, do not contain harmful impurities and exceed the quality of world analogues


2021 ◽  
Vol 845 (1) ◽  
pp. 012096
Author(s):  
E I Popova ◽  
K V Bryksina

Abstract Fruits and vegetables are the most popular raw ingredients that act as a source of dietary fiber and other biologically active substances that benefit the human body. The vast majority of nutrients enter the human body with food, so the daily ration of an average person should include those useful products that can provide the body with the necessary amount of biologically active substances. Vegetable-based raw materials containing vitamins, minerals, antioxidants, dietary fibers used in the production of food products are considered a source of functional properties. A priority area of product technologies is the search for technological methods for the production of functional food products with a given biological value (high BAS and antioxidant content). The paper presents recipes and innovative technology for the production of functional syrups based on Viburnum fruits, as well as the evaluation of consumer characteristics, nutritional value and functional orientation of the corresponding products.


2021 ◽  
Vol 10 (3) ◽  
pp. 95-99
Author(s):  
N. A. Salnikova ◽  
Yu. V. Shur ◽  
A. A. Tsibizova

Introduction. Currently, close attention in the field of pharmacy and medicine is directed to the search for new sources of biologically active substances of various origins, including vegetable. The woody plant Elaeagnus argentea is considered as a promising source. Despite its widespread use in the food industry, folk medicine as an anti-inflammatory, restorative, antimicrobial agent, the chemical composition of this plant has not been sufficiently studied.Aim. Qualitative and quantitative determination of the main groups of biologically active substances (BAS) in the raw material (leaves) of Elaeagnus argentea growing on the territory of the Astrakhan region for further development of a methodology for standardizing the raw material of this plant.Materials and methods. The leaves of Elaeagnus argentea were harvested in the spring in the eastern part of the delta of the Astrakhan region (Volga region). Drying of raw materials was carried out in natural conditions. In the study, unified methods were used: to determine the amount of flavonoids and saponins, the spectrophotometric method was used, ascorbic acid - the titrimetric method. For the analysis of flavonoids in the leaves of Elaeagnus argentea, an aqueous-alcoholic solution of 70 % concentration was used as an extractant. The quantitative content of flavonoids was determined in the obtained raw material extract in terms of luteolin-7-glucoside. The amount of saponins in the leaves of Elaeagnus argentea was determined in terms of oleanolic acid. 96 % ethanol was used as an extractant. The quantitative determination of ascorbic acid in the aqueous extract of crushed raw materials was carried out by titrimetry based on the ability to reduce 2,6-dichlorophenolindophenol.Results and discussion. In the pharmacognostic study of biologically active substances in the leaves of Elaeagnus argentea, the content of ascorbic acid was found to be at least 0.32 %, the amount of flavonoids in terms of luteolin-7-glucoside was at least 1.92 %; saponins - 2.38 %; which indicates the need for a more detailed study of the phytochemical composition of other morphological groups of the plant Elaeagnus argentea growing in the Astrakhan region.Conclusion. The data obtained during the study can be used to confirm the quality of raw materials (leaves) of Elaeagnus argentea. A more detailed study of leaves for the presence of other groups of biologically active substances will make it possible to use the obtained data for the development of regulatory documents for medicinal plant raw materials «Elaeagnus argentea leaves».


Author(s):  
Алла Георгиевна Куклина ◽  
Наталья Степановна Цыбулько

Проведен фитохимический анализ цветков, плодов и листьев по содержанию биологически активных веществ на двух видах клена: инвазионном Acer negundo L. и аборигенном A. platanoides L. (Sapindaceae Juss). У 40 образцов, собранных в 5 популяциях Московского региона, найдены сумма флавоноидов, содержание аскорбиновой кислоты и суммы органических кислот по стандартным методикам Государственной фармакопеи РФ. В данном исследовании впервые обнаружено, что максимальное накопление флавоноидов (1,75-1,94 мг%) фиксируется в мужских цветках A. negundo. Содержание витамина С в цветках (до 53 мг%), плодах и листьях A. negundo на 25-30 % больше, чем в аналогичных органах у A. platanoides. По мере созревания плодов содержание флавоноидов, аскорбиновой кислоты и органических кислот (до 8 %) у инвазионного и аборигенного видов клена снижается почти в 1,5 раза. Листья мужских и женских особей A. negundo по насыщенности флавоноидами, витамином С и органическими кислотами не отличаются. Выявлено, что цветки A. negundo и A. platanoides, являются наиболее перспективным источником биологически активных веществ - флавоноидов, включая катехины, антоцианы, лейкоантоцианы и флавонолы, а также аскорбиновой кислоты. Сбор растительного сырья рекомендуется проводить в экологически чистых фитоценозах, поскольку известно о способности растений A. negundo аккумулировать из почвы и воздуха тяжелые металлы. A phytochemical analysis of flowers, fruits, and leaves revealing the biologically active substances in two types of maple: invasive Acer negundo L. and native A. platanoides L. (Sapindaceae Juss) was carried out. In 40 samples collected in 5 populations of the Moscow Region, a sum of flavonoids, the presence of ascorbic acid, and organic acids were found according to the standard methods of the State Pharmacopoeia of the Russian Federation. We discovered for the first time that the maximum accumulation of flavonoids (1.75-1.94 mg%) is in male flowers of A. negundo. The content of vitamin C in flowers (up to 53 mg%), fruits, and leaves of A. negundo is 25-30% higher than in similar organs in A. platanoides. As the fruit ripens, the content of flavonoids, vitamin C, and organic acids (up to 8%) in invasive and native species of maple decreases by almost 1.5 times. The leaves of male and female trees of A. negundo do not differ in saturation with flavonoids, vitamin C, and organic acids. Flowers of A. negundo and A. platanoides are the most promising source of biologically active substances - flavonoids, including catechins, anthocyanins, leukoanthocyanins, and flavonols, as well as ascorbic acid. The collection of plant substances is recommended in ecologically clean phytocenoses since A. negundo can accumulate heavy metals from soil and air.


Author(s):  
T. Lebedenko ◽  
G. Krusir ◽  
H. Shunko ◽  
H. Korkach

Currently, the creation of a range of new products that improve human nutritional status is a timely and urgent problem facing scientists in the food industry. In food technology it is effective to use functional ingredients that have enhanced protective and improved technological properties. It is possible to confidently attribute the components of plant origin, namely, dietary fiber and plant phospholipids, to such substances. Phospholipids are a group of essential substances that are irreplaceable and enter the human body only with food. Phospholipids cannot be formed independently in the human body, but perform important functions that have a positive effect on the body: they restore cell walls; they are involved in lipid metabolism, as well as reducing the risk of cholesterol formation; they actively participate in the delivery processes of biologically active substances to cells, etc. A rich source of biologically active substances are flax seeds, its medicinal properties have been known for centuries and are recognized by official medicine. The influence of flax seed mucilage and phospholipids in the formulation of red main sauce on rheological and physical parameters was investigated. Samples of sauces with replacement of 50 and 100% of flour for flax polysaccharides and 30 % of animal fat for vegetable phospholipids were developed. The change of effective viscosity and density of the developed sauces is investigated. It was found that at a shear rate of 0.3333 s-1 the viscosity of the control sample is 14.32 Pa·s, of the same one with the replacement of 50 % flour and 10 g of fat and 100 % flour and 10 g of fat, respectively, is 16.49 and 19.6 Pa·s. This replacement in the experimental samples is accompanied by a slight increase in density. In order to substantiate the functional orientation of sauces in the introduction of herbal additives, studies were conducted to determine the antioxidant activity (AOA) of the developed sauces and their components. It was found that the maximum AOA a sauce with herbal additives has, which for 90 minutes is able to “quench” 89 % of the radicals formed in these conditions in the model system. Adding sauces to ready meals, even in small quantities, help to increase the nutritional value and attractiveness of the consumed food. Therefore, the effect of additives on the consumer characteristics of sauces was investigated. It is established that the developed types of sauces have a characteristic structure, creamy and homogeneous consistency, pronounced taste and aroma. The conducted complex of researches allows to state with confidence that use of available vegetable raw materials instead of the ingredients possessing the raised food value, allows to reduce energy value and to expand the range of sauces of functional orientation for restaurant enterprises.


2018 ◽  
pp. 59-62
Author(s):  
Z.S. Zobkova ◽  
◽  
T.P. Fursova ◽  
D.V. Zenina ◽  
A.D. Gavrilina ◽  
...  

Author(s):  
SHAPOVALOVA Nataliia ◽  
VEZHLIVTSEVA Svitlana ◽  
ANTIUSHKO Dmytro

Background. The deficiency of essential nutrients in the human body is the root cause of reduced efficiency, general resistance of the body to various diseases. That is an urgent problem in the context of the spread of coronavirus infection. In this regard, the search for opportunities to meet the needs of the population in food products that contain biologically active substances (BAS) through the use of high-value and at the same time available plant raw materials is extremely important. Marigolds attract special attention among such raw materials. The aim of the work is to study the chemical composition and evaluate the consumer properties of Tagetes L. varieties, zoned in the Kiev region, to prove the possibility of increasing the biological value of pasta by adding to their recipe powder from marigold inflorescences. Materials and methods. The object of research is pasta of B group (noodles). Inflorescences of marigolds of different varieties gathered during the flowering period in July-September 2019–2020 in the territory of Vasylkiv and Obukhiv districts of Kyiv region, Ukraine were used as enriching plant raw materials. The quality of finished pasta was assessed by organoleptic and physicochemical indexes (moisture content and titratable acidity). The content of mineral elements in the fine powder of marigold inflorescences was determined on the EXHERT-3L device, the quantitative composition and BAS identification – by spectrophotometric analysis on the device Specord-200 Analytic Jena UV-vis. The integrated quality index of pasta products was calculated taking into account the importance factors of individual indexes. Results. Comparing the obtained results, we can state that all the studied varieties – Gold Kopfen, Orange Flame, Hawaii, Equinox are characterized by high biological value, regardless of the area of collection. As a result of calculated and experimental studies it was determined that the addition of 7 % of the supplement does not provide the required supply of vitamins and minerals. Replacing the flour in the recipe by 20 % significantly improves the mineral composition, but deteriorates the taste and aromatic properties of pasta. The most optimal and rational amount of added additive is 15 %, as it improves the organoleptic properties of pasta and enriches their BAS. Conclusion. The usage of non-traditional vegetable raw materials, in particular powder from marigold inflorescences, which is rich in biologically active substances, allows not only to expand the range, improve the quality of finished products, but also increase the biological value of the product. The optimal amount of additive –powder from marigold inflorescences, was defined. It is 15 % by weight of flour in the production of noodles. This concentration allows not only to improve the organoleptic properties, but also to increase the content of biologically active substances – vitamins, micro- and macronutrients. The usage of non-traditional natural raw materials (powder from marigold inflorescences) allows to abandon artificial colorsand give the finished pasta a pleasant colorand aroma.


2018 ◽  
Vol 26 (2) ◽  
pp. 296-304
Author(s):  
Elena A. Laksaeva

An interest in plants of Amelanchier genus is stimulated by the necessity of search for plant sources rich in biologically active substances and possessing the ability to influence different physiological processes in a human organism. The article gives information about places of growth of different species of Amelanchier (Amelanchier Medic) and about the fact that its fruits contain high concentration of free sugars, relatively low level of organic acids, sufficient amounts of various vitamins and provitamins and a complex of mineral substances. Nutritive and biological value of Amelanchier fruits in complex may produce a beneficial effect on different biochemical and physiological processes of normal vital activity of a human organism. The data are given about a positive influence of enteral introduction of polysaccharides isolated from Amelanchier fruits, on different physiological processes that enhance adaptational reserves of an organism of experimental animals. In particular, it was shown that watersoluble polysaccharide complex (WSPC) of Amelanchier fruits activates erythropoiesis increasing the amount of erythrocytes and hemoglobin in blood of experimental animals and raises concentration of iron. WSPC improves physical working capacity and increases the body mass of experimental animals. Addition of watersoluble polysaccharide complex of Amelanchier fruits to blood of a healthy donor increases thermal, osmotic and peroxide resistance of erythrocyte membranes thus protecting cells against damage under action of adverse factors in the experiment. Fruits of plants of Amelanchier genus may be recommended to be used as a food additive or a product in dietary and prophylactic therapy.


2021 ◽  
Vol 38 ◽  
pp. 00033
Author(s):  
Tatiana Fomina ◽  
Tatiana Kukushkina

The content of the major groups of biologically active substances in flowers of 7 Campanula species, cultivated in the forest-steppe of Western Siberia, were investigated for the first time. Freshly collected flowers were found to contain 0.06-0.15 % of catechins, 0.31-3.17 % of flavonols, 3.31-6.47 % of tannins, 3.10-15.57 % of pectic substances, and 0.66-16.4 mg% of carotenoids calculated per absolute dre weight of raw material. The amount of ascorbic acid was 36.9-114.0 mg% per wet weight. The data indicate that Campanula flowers are promising for the production of phenolic compounds, the development of food additives and fresh consumptions a seasoning.


Author(s):  
Н.В. БОГОЛЮБОВА ◽  
Р.А. РЫКОВ

Изучен способ нормализации обменных процессов и повышения продуктивности молочных коров при использовании комплекса биологически активных веществ. Эксперимент проведен на коровах голштинской породы (n=12) с продуктивностью за предыдущую лактацию 9000 кг молока. За 20 дней до отела и в течение 65 дней после коровы опытной группы получали комплексную кормовую добавку (ККД), включающую минерал шунгит, холин в «защищенной» форме, пробиотик «Целлобактерин+» и жмых льняной, в количестве 200 г на голову в сутки в смеси с концентратами. Для изучения обменных процессов в организме коров (n=5) в конце опыта отобраны пробы крови и определены основные биохимические показатели. Для оценки продуктивных качеств проводили контрольные дойки. Отмечено повышение в крови коров опытной группы концентрации общего белка на 2,8%, глюкозы — на 26,8% (P≤0,01), фосфолипидов — на 15,2% (P≤0,05), снижение мочевины на 23,6% (P≤0,05), билирубина — на 23,8% (P≤0,01), активности АЛТ на 13,11 (P≤0,05), АСТ — на 14,6% (P≤0,05). Использование ККД позволило снизить и привести в норму активность ферментов: лактатдегидрогеназы (ЛДГ), гаммаглутаминтрансферазы (ГГТ), креатинкиназы. Повышение интенсивности обменных процессов и нормализация показателей, характеризующих работу печени с использованием в питании комплекса биологически активных веществ ферментно-пробиотического, антиоксидантного и липотропно-гепатопротекторного действия, привело к повышению молочной продуктивности. A method of normalizing metabolic processes and increasing the productivity of dairy cows using a complex of biologically active substances has been studied. The experiment was carried out on Holstein cows (n = 12) with a productivity for the previous lactation of 9000 kg of milk. 20 days before calving and within 65 days after the cows of the experimental group received a complex feed supplement (CFS), including the mineral shungite, choline in a "protected" form, the probiotic "Cellobacterin +" and flaxseed cake, in an amount of 200 g per head per day mixed with concentrates. To study metabolic processes in the body of cows (n = 5), at the end of the experiment, blood samples were taken and the main biochemical parameters were determined. To assess the productive qualities, control milking was carried out. An increase in the blood of cows of the experimental group was noted in the concentration of total protein by 2.8%, glucose - by 26.8% (P≤0.01), phospholipids — by 15.2% (P≤0.05), a decrease in urea by 23,6% (P≤0.05), bilirubin - by 23.8% (P≤0.01), ALT activity by 13.11 (P≤0.05), AST — by 14.6% (P≤ 0.05). The use of CFS made it possible to reduce and normalize the activity of enzymes: lactate dehydrogenase (LDH), gamma glutamine transferase (GGT), creatine kinase. An increase in the intensity of metabolic processes and the normalization of indicators characterizing the work of the liver with the use of a complex of biologically active substances of enzymatic-probiotic, antioxidant and lipotropic-hepatoprotective action in the diet led to an increase in milk productivity.


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