SELECTION OF AN EXTRACTANT FOR ISOLATION OF BIOLOGICALLY ACTIVE COMPOUNDS FROM A HEDYSARUM NEGLECTUM
Global technological progress is one of the main problems of ecosystem deterioration. The high concentration of industrial enterprises contributes to the development of the region's environmental crisis and the decline in public health. An increase in the environmental pollutant contributes to the activation of oxidative stress in the human body, which plays a key role in the pathogenesis of a number of chronic diseases (cancer, diabetes, cardiovascular diseases, etc.), leading to a decrease in life expectancy and early aging of the body. To increase the adaptive abilities of the human body, it is important to use plant raw materials. A promising source of natural nutrients is the Hedysarum neglectum Ledeb. In order to obtain the extract of the forgotten kopek, the method of extraction was used at room temperature with a reverse refrigerator for 2 hours. In this study, to determine the working parameters of extraction (type of extractant, concentration of extractant), a selection of various extractive substances was selected. The efficiency of the extraction process was determined by two parameters: extractivity and yield of biologically active substances. The maximum extractivity was observed when using 50 % ethyl alcohol. To determine the working concentration of the water-ethanol mixture, the prepared extracts were analyzed for the content of flavonoids, tannins, and ascorbic acid. The maximum yield of biologically active substances (flavonoids 419.02±3.58 mg%, tannins 8.10±1.03 %, ascorbic acid 691.04±3.62 mg/kg) was observed during extraction with 50% water-ethanol mixture. The obtained results suggest that the water-ethanol extract of Hedysarum neglectum Ledeb. it is a potential source of natural biologically active substances for creating functional food products.