scholarly journals Pharmacological evaluation of wound healing activity of herbal based formulation “Amree Plus®” in diabetic rats

2017 ◽  
Vol 6 (1) ◽  
pp. 1-10
Author(s):  
Sunita Thakur ◽  
◽  
Isha Dhamija ◽  
Sandeep Kumar ◽  
Anil Kumar Sharma ◽  
...  
Keyword(s):  
Wound Healing ◽  
Diabetic Rats ◽  
Proline Content ◽  
Wound Contraction ◽  
Total Protein Content ◽  
Delayed Wound Healing ◽  
Polyherbal Formulation ◽  
Wound Model ◽  
Herbal Formulation ◽  
Wound Healing Activity

Delayed wound healing is producing high economic burden on the society and is generally associated with Diabetes mellitus or related complications thus needs safe treatment like herbal formulation. „Amree Plus® (an anti-diabetic ayurvedic formulation)‟ was evaluated for wound healing potential in normal and diabetic rats. The effect of polyherbal formulation Amree plus ® on wound healing has been studied in diabetic and normal animals and compared with standard (Chymoral Forte) using excision wound model. Diabetes was induced by administration of single dose of Streptozotocin 55 mg/kg i.p. The biochemical parameters i.e. hydroxyl proline content, total protein content, glutathione and superoxide dismutase (SOD) were also estimated. Rats treated with Amree Plus® at both doses i.e. 1200 and 5400 mg/kg dose per day had significantly high wound contraction both in diabetic and normal rats (88.91 & 95.05 %, respectively) in comparison to respective control. Hydroxyproline content of the granulation tissue and anti-oxidants (glutathione and SOD) level were also showing same pattern in Amree Plus® 5400 mg/kg treatment group with respect to controls. Wound contraction and other biochemical indicators results with animals Amree Plus® (1200 mg/kg) treated were found to be statistically equivalent to standard chymoral forte dose (36 kAU/kg) while Amree Plus® 5400 mg/kg dose showed statistically higher results than standard. Amree Plus®, an established antidiabetic formulation, was also showing healing of the wound in normal animals along with diabetic animals. So this finding should be confirmed further for more precision with large number of animals and small progression of doses.

scholarly journals EVALUATION OF WOUND HEALING ACTIVITY OF METHANOLIC EXTRACT OF SMILAX WIGHTII (A. DC.) IN WISTAR ALBINO RATS

2014 ◽  
Vol 1 (1) ◽  
pp. 73-77
Author(s):  
Uma Maheswari P ◽  
Shalimol A ◽  
Arumugasamy A ◽  
Udhaya Sankar M.R
Keyword(s):  
Wound Healing ◽  
Dry Weight ◽  
Wound Contraction ◽  
Albino Rats ◽  
Standard Drug ◽  
Excision Wound ◽  
Wound Model ◽  
Wistar Albino Rats ◽  
Incision Wound ◽  
Wound Healing Activity

The present experimental study was designed to evaluate the wound healing activity of methanolic extract of Smilax wightii A. DC. on incision and excision wound models in Wistar albino rats. The parameters studied were wound breaking strength, wound contraction area, epithelialization period, granulation tissuewet, dry weight and hydroxyproline content in incision wound model, percentage of wound contraction and period of epithelialization in excision wound model. The rats were administered topically with 100mg/kg b.wt. (low dosage), 200mg/kg b.wt. (moderate dosage) and 500mg/kg b.wt. (high dosage) of methanolicextract of Smilax wightii (MESW). The activity of the extract treated groups were compared with that of the control 1% Spirit. Framycetin sulphate 0.2% w/w was used as the standard drug. In incision wound model, there was a significant increase in the wound breaking strength in all the experimental groups treated withMESW than that of the control. Similarly, significant (P<0.001) decrease in wound contraction area and period of epithelialization were also observed in the test group animals treated with MESW and the standard drug treated groups when compared to that of the control. , a significant increase was observed in granulation tissue wet and dry weight and hydroxyproline content in the test groups treated with MESW compared to the control. In exicision wound model, there was a significant increase (P<0.01) in the percentage of wound contraction and decrease in period of epithelialization in the experimental groups treated with 200mg/kgb.wt. (moderate dosage) and 500mg/kgb.wt (high dosage) of MESW. The extract treated groups showed significant improvement in all the wound healing parameters of incision, and excision wound models.

EVALUATION OF EXCISIONAL AND INCISIONAL WOUND HEALING ACTIVITY OF ELECTROHOMEOPATHIC DRUG (SPAGYRIC ESSENCE) GREEN ELECTRICITY IN RATS

2020 ◽  
pp. 72-75
Author(s):  
PRASANT KUMAR SABAT ◽  
SWETA PRIYADARSINI PRADHAN ◽  
RANDEEP PATRO
Keyword(s):  
Wound Healing ◽  
Tensile Strength ◽  
Wound Contraction ◽  
Control Group ◽  
Albino Rats ◽  
Green Electricity ◽  
Wound Model ◽  
Wound Protection ◽  
Incision Wound ◽  
Wound Healing Activity

Objective: The current research is an attempt to evaluate the wound healing activity of Electrohomeopathic drug Green electricity (GE) in Wistar rats. No data have been reported till date on the wound healing effect of GE. Hence the present research focuses on the scientific investigation of the wound healing activity of GE in rats. Methods: Excision and incision wounds were inflicted upon albino rats. In the excision wound model, the wound contraction, Scar residue, area and time of complete epithelization were measured subsequently on day 0, 3,6,9,12,15, 18 and 21 after inflicting the wound. Wound contraction was calculated as a percentage change in the initial wound size. In the incision wound model, the tensile strength was measured on the 10th post wounding day. Results: It was noted that the effect produced by the GE Showed significant wound healing (p<0.01) in all wound models when compared to the control group. In the case of the excision model, GE statistically increases the wound contraction rate and in the case of the incision model GE showed high tensile strength as compared to the control group. In both cases the epithelization period was significantly decreased as compared to control. Almost 100% wound protection was achieved by GE in 21 d as compared to control. Conclusion: GE (ointment) showed significant wound healing activity (p<0.01) in both the excised wound model and incision wound model in rats.

Wound healing activity of Terminalia bellerica Roxb. and gallic acid in experimentally induced diabetic animals

2019 ◽  
Vol 17 (2) ◽  
Author(s):  
Manish Pal Singh ◽  
Avneet Gupta ◽  
Siddhraj Singh Sisodia
Keyword(s):  
Wound Healing ◽  
Gallic Acid ◽  
Dead Space ◽  
Diabetic Rats ◽  
Fruit Extract ◽  
Standard Drug ◽  
Terminalia Bellerica ◽  
Wound Model ◽  
Wound Healing Activity ◽  
Experimentally Induced

AbstractBackgroundDiabetic wound are the major problem of society because of its delay healing of wound in hyperglycemia patient. The target of this study need to know the gallic acid compelling synergistically wound healing in diabetic animals as it is available in fruit extract or is more effective in given pure form in diabetic animals.MethodsThe wound healing effects of fruit extract Terminalia bellerica Roxb. and gallic acid were evaluated in experimentally induced hyperglycemic animals. In this investigation, excision and dead space wound models were used. The contraction wound %, re-epithelialization days, area of scar, histopathological examination of tissue, hydroxyproline level and weight of tissue granuloma were evaluated in this study.ResultsTerminalia bellerica fruit extract (400 mg/Kg) and gallic acid (200 mg/Kg) have been ingested by orally in streptozotocin induced diabetic rats. They were founded significantly (p<0.05) improved percentage wound contraction and decreased the size of scar area and days of re-epithelialization in excision wound model. Also, they were improved the level of hydroxyproline level in diabetic rats as compared to diabetic control group in dead space wound model. Standard drug vitamin C was also founded significantly improved wound healing activity in rats. These results suggested that treated groups accelerated wound healing activity in diabetic rat.ConclusionsThe observations and results obtained in the present study indicated the wound healing efficacy of fruit ethanolic extract and gallic acid in diabetic animals. Yet, the wound healing effect was increasingly reported with gallic acid.

WOUND HEALING ACTIVITY OF TRIPHALA IN EXPERIMENTALLY INDUCED DIABETIC RATS

INDIAN DRUGS ◽  
2017 ◽  
Vol 54 (04) ◽  
pp. 70-75
Author(s):  
M. P. Singh ◽  
S Vashisht ◽  
V. Chawla ◽  
P. Kumari ◽  
Keyword(s):  
Wound Healing ◽  
Oral Administration ◽  
Dead Space ◽  
Diabetic Rats ◽  
Wound Contraction ◽  
Control Group ◽  
Excision Wound ◽  
Wound Model ◽  
Diabetic Wound Healing ◽  
Healing Property

Aim of the present study was to explore the wound healing potential of Triphala extract. Triphala ointment (15% w/w) was used in the study for excision wound model. For dead space wound model Triphala extract (200 mg/kg) was administered orally. Extract of Triphala topically applied in excision wound model was found to possess wound healing property. On day 11th, the extract treated animals showed wound contraction to the extent of 87.63% and 94.77%, compared with control group of animals. In dead space wound model, oral administration of the extract was found to increase the wet and dry granuloma weight of tissue. The extract treated animals showed high quantity of hydroxyproline content when compared with the control group animals. The topical and oral administration of Triphala extract promotes diabetic wound healing in streptozotocin induced diabetic rats with increased rate of wound contraction and collagen turnover.

scholarly journals EVALUATION OF WOUND HEALING ACTIVITY OF POLYHERBAL FORMULATION

2017 ◽  
Vol 9 (6) ◽  
pp. 12
Author(s):  
Madhuri A. Theng ◽  
G. R. Sitaphale ◽  
K. R. Biyani
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Soxhlet Extraction ◽  
Wound Healing Process ◽  
Burn Wound ◽  
Butea Monosperma ◽  
Polyherbal Formulation ◽  
Wound Model ◽  
Acacia Arabica ◽  
Wound Healing Activity

Objective: The present study describes the anti-microbial acivity of Acacia arabica and Butea monosperma bark extract.Methods: For this purpose aqueous extract of bark were prepared by “Soxhlet extraction method”. The experimentally induced burn wound model in rats by “Excision method”.Results: As a result of this study it was found that the extract of bark generally revealed antimicrobial and wound healing activity.Conclusion: The result of the study suggest that the Acacia arabica and Butea monosperma bark of polyherbal gel effective in accelerating wound healing process.

The Extract of Lycium depressum Stocks Enhances Wound Healing in Streptozotocin-Induced Diabetic Rats

2017 ◽  
Vol 16 (2) ◽  
pp. 85-93 ◽  
Author(s):  
Siamak Naji ◽  
Leila Zarei ◽  
Masoumeh Pourjabali ◽  
Rahim Mohammadi
Keyword(s):  
Wound Healing ◽  
Treatment Group ◽  
Plant Material ◽  
Diabetic Rats ◽  
Male Rats ◽  
Impaired Wound Healing ◽  
Wound Model ◽  
Methanolic Extracts ◽  
Powder Extract ◽  
Wound Healing Activity

In diabetes, impaired wound healing and other tissue abnormalities are considered major concerns. The aim of the present study was to assess the wound-healing activity of methanolic extracts of the extract of Lycium depressum leaves. A total of 60 healthy male Wistar diabetic rats weighing approximately 160 to 180 g and 7 weeks of age were randomized into 10 groups for incision and excision wound models: sham surgery group (SHAM), including creation of wounds and no treatment; base formulation group (FG) with creation of wounds and application of base formulation ointment; treatment group 1 (TG1) with 1 g of powder extract of the plant material in ointment; treatment group 2 (TG2) with 2 g; and treatment group 4 (TG3) with 4 g of powder extract of the plant material in ointment. A wound was induced by an excision- and incision-based wound model in male rats. The mature green leaves of L depressum were collected and authenticated. Extractions of dried leaves were carried out. For wound-healing activity, the extracts were applied topically in the form of ointment and compared with control groups. The healing of the wound was assessed based on excision, incision, hydroxyproline estimation, and biomechanical and biochemical studies. The extract of L depressum leaves enhanced wound contraction, decreased epithelialization time, increased hydroxyproline content, and improved mechanical indices and histological characteristics in treatment groups compared with SHAM and FG ( P < .05). These findings permit the conclusion the extract of L depressum benefits parameters of wound healing in a diabetes induced model

scholarly journals Phytosomes of Naphthoquinone enriched Extract of Root bark of Onosma echioides exhibit wound healing activity in Rats

2021 ◽  
pp. 474-483
Author(s):  
JEEJA PANANCHERY ◽  
Chhaya Gadgoli
Keyword(s):  
Wound Healing ◽  
Tensile Strength ◽  
Granulation Tissue ◽  
Petroleum Ether Extract ◽  
Treated Group ◽  
Wound Contraction ◽  
Root Bark ◽  
Wound Model ◽  
Wound Healing Effect ◽  
Wound Healing Activity

The present study is aimed at evaluation of phytosomal gel of the petroleum ether extract of root bark of Onosma echiodes for wound healing activity in rats. Extract of root bark of O. echioides was standardized by isolated naphthoquinone dimer using HPTLC. Phytosomes (equivalent to 2% w/w of naphthoquinones) of the standardized extract were prepared by thin film hydration technique. The wound healing efficacy of the formulation was evaluated in rats by inflicting excision and incision wounds followed by treatment of the wounds topically. The parameters evaluated for healing included determination of breaking strength and tensile strength of healed skin for incision model and percentage wound contraction, hydroxyproline content, granulation tissue free radicals and catalase in excision wound model. The formulation treated group showed a significant healing (p<0.005) of both the excision and incision wounds with respect to wound contraction and tensile strength respectively, as compared to vehicle treated group. The oxidative stress of the granulation tissue was also found to be reduced as indicated by reduced lipid peroxidation and increase in catalase activity. The phytosomal gel of O. echioides effectively exhibited wound healing effect.

scholarly journals Evaluation of polyherbal ointment for wound healing activity in Wistar rats

2018 ◽  
Vol 8 (6-s) ◽  
pp. 26-31
Author(s):  
Shilpa S. Kolhe
Keyword(s):  
Wound Healing ◽  
Wistar Rats ◽  
Breaking Strength ◽  
Skin Irritation ◽  
Wound Contraction ◽  
Alcoholic Extract ◽  
Polyherbal Formulation ◽  
Wound Healing Activity

The aim of present study to formulate, evaluate and to check the wound healing activity of newly prepared polyherbal formulation (ointment) .Herbal ointment containing hydrochloric extracts of plants Psorolea corylifalia, Achryanthes aspera ,was formulated as ointment and the hydro alcoholic extract was prepared by maceration method. These Formulations were evaluated for the following parameters: pH, Spread ability, grittiness, skin irritation study, stability. The wound healing activity is assessed by the rate of wound contraction, Period of epithelisation and skin breaking strength. Keywords: Herbal Ointment, Wound Healing Activity.

scholarly journals Wound healing potentials of herbal ointment containing Calendula officinalis Linn. on the alteration of immunological markers and biochemical parameters in excision wounded animals

2020 ◽  
Vol 6 (1) ◽  
Author(s):  
Shobana Gunasekaran ◽  
Agnel Arul John Nayagam ◽  
Rameshkannan Natarajan
Keyword(s):  
Wound Healing ◽  
Biochemical Parameters ◽  
Lipid Peroxide ◽  
Wound Contraction ◽  
Tnf Alpha ◽  
Albino Rats ◽  
Calendula Officinalis ◽  
Wound Model ◽  
Immunological Markers ◽  
Wound Healing Activity

Abstract Objective The present study was designed to investigate the in vivo wound healing activity of herbal ointment prepared from Calendula officinalis Linn. on excision wounded rats. Materials and methods The excision wound model was employed for wound healing activity in albino rats. Healthy albino rats (150–200 g) of either sex were taken for excision wound model. Animals were divided into five groups of six animals in each. Group I served as normal control, Group II served as excision wounded control without treatment and Group III, IV served as excision wounded rats were treated with herbal ointment of two different doses (10% and 20%) applied topically for 14 days and group V served as excision wounded animals treated with reference ointment soframycin. Healing potential was evaluated by the rate of wound contraction, immunological markers like IL-6(Interleukin 6), TNF-alpha (Tumor necrosis factor -α), PDGF (Platelet Derived Growth Factor) and EGF (Epidermal Growth Factor), lipid peroxide (LPO), superoxide dismutase (SOD), and biochemical parameters like hydroxyproline, hesosamine, and tissue protein. Results The topical application of herbal ointment treated groups showed increase in the levels of growth factors such as PDGF and EGF hydroxy proline, hesosamine, tissue protein, SOD and wound contraction and the ointment normalized the levels of lipid peroxide, IL-6, TNF-alpha compared than that of excision wounded animals. Conclusion From the above results, it was concluded that the topical application of herbal ointment exhibited significant wound healing activity in excision wounded rats as evidenced by increased wound contraction and collagen synthesis.

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