scholarly journals Bacterial Load Reduction in Guheswori Sewage Treatment Plant, Kathmandu, Nepal

2019 ◽  
Vol 15 (1) ◽  
pp. 40-44 ◽  
Author(s):  
Bikram Gautam ◽  
Anjita Rajbhanshi ◽  
Rameshwar Adhikari

Background: Water sources such as lakes, ponds, river etc. have been continuously contaminated by the   micro organisms and chemicals. The former can pose a significant threat to human health. This work aims at detecting the bacterial load before and after the sewage treatment and hence isolating pathogens from the sewage before primary treatment and secondary treated effluent. Methods: Grab sampling (50mL sewage before primary treatment and secondary treated effluent) was performed for 20 days in the Guheswori  sewage treatment plant. The reduction in microbial load was determined through heterotrophic plate count. Pathogens were screened from the effluent obtained from the secondary treatment plant. Results: Bacterial load reduction was found to be about 48.02% on average. The observed bacterial load reduction might have been caused by bacteriophage flocculation and sedimentation. Pathogens isolated from the treated effluent were Escherichia coli, Salmonella Typhi, Enterococcus faecalis, Staphylococcus aureus, Coagulase negative Staphylococcus (CONS), Citrobacter fruendii, Enterobacter aerogenes, Proteus mirabilis, P. vulgaris, Pseudomonas  aeruginosa. Conclusions: It has been found that the sewage treatment plant helps to reduce the bacterial load which is, however, not capable of effluent polishing where all pathogens are killed. 

2018 ◽  
Vol 4 (2) ◽  
pp. 465-470 ◽  
Author(s):  
Bikram Gautam ◽  
Lomas Aryal ◽  
Sachana Adhikari ◽  
Manoj Rana ◽  
Anjita Rajbhanshi ◽  
...  

Background: Waste water contains microorganisms which are continuously shed in the feces. These microorganisms especially bacteria might acquire antibiotic resistance and pose a significant threat to human health. Therefore, this work aims at isolating bacteriophage capable of infecting the isolated bacteria. Methodology: For this purpose, the grab sampling was performed at the Guheswori sewage treatment plant from the inlet in the primary treatment plant and from the outlet of the secondary treatment plant. For the isolation of bacteriophage, bacteriophage in the sewage was first enriched in an isolated pathogen, then filtered and then subjected to the isolates in the nutrient agar. Results: Pathogens like Escherichia coli, Salmonella Typhi, Enterococcus faecalis, Staphylococcus aureus, Coagulase negative Staphylococcus (CONS), Citrobacter fruendii, Enterobacter aerogenes, Proteus mirabilis, P. vulgaris, Pseudomonas aeruginosa were screened. Bacteriophage was able to infect E. coli (p < 0.001), S. Typhi (p < 0.001), E. faecalis (p = 0.182); and unable to infect S. aureus, CONS, C. fruendii, E. aerogenes, P. mirabilis, P. vulgaris, P. aeruginosa. Conclusion: Bacteriophage are able to infect and kill pathogens like E. coli, S. Typhi, E. faecalis and unable to infect S. aureus, CONS, C. fruendii, E. aerogenes, P. mirabilis, P. vulgaris, P. aeruginosa. Among all other reasons of lowering bacterial load, bacteriophages could also be one of the confounding factor. Such bacteriophage able to infect and undergo lytic cycle could be used in phage typing.


2012 ◽  
Vol 72 (4) ◽  
pp. 839-846 ◽  
Author(s):  
AD. Vecchia ◽  
JD. Fleck ◽  
M. Kluge ◽  
J. Comerlato ◽  
B. Bergamaschi ◽  
...  

In order to verify the microbial quality of the influents and effluents of one STP from southern Brazil, an eight-month survey was conducted to examine the presence of total and fecal coliforms and of adenovirus (HAdV), enterovirus (EV), genogroup A rotaviruses (GARV) and Torque teno virus (TTV), in treated effluent samples from São João/Navegantes STP, Porto Alegre (Brazil). A total of 16 samples were collected, eight of influent (raw sewage, prior to treatment), and the other eight of the effluent (post-treatment sewage). Total and fecal coliform levels ranging from 3.6 × 10(4) to 4.4 × 10(7) MPN/100 mL and 2.9 × 10³ to 1.7 × 10(7) MPN/100 mL, were detected in all samples. In raw sewage, HAdV (25%) and GARV (28.6%) viral genomes were detected. The analysis of effluent samples revealed the presence of HAdV (50%), EV (37.5%), and TTV (12.5%) genomic fragments. All samples, regardless of the month analysed, presented detection of a least one virus genus, except for in April. Higher virus detection rates were observed in treated sewage samples (62.5%), and in 80% of them (effluent positive samples) HAdV was detected. Results showed that improvements in sewage monitoring and treatment processes are necessary to reduce the viral and bacterial load on the environment in southern Brazil. To the knowledge of the authors, this is the first study showing the monitoring of viral genomes in influent and effluent samples from a STP located in Porto Alegre (Rio Grande do Sul, Brazil), southern Brazil.


1998 ◽  
Vol 38 (2) ◽  
pp. 25-32 ◽  
Author(s):  
C. W. Chu ◽  
C. S. Poon ◽  
R. Y. H. Cheung

Chemically Enhanced Primary Treatment (CEPT) or Chemically Assisted Primary Sedimentation (CAPS) is being employed at the new sewage work on Stonecutters Island as part of the Strategic Sewage Disposal Scheme (SSDS) in Hong Kong. CAPS involves the use of chemical coagulants (such as lime or ferric chloride) to induce coagulation or flocculation and let these finely-divided particles form large aggregates (floc) so that they can settle out within a reasonable period of time. In this study, five sludge samples collected from different sewage treatment plants in Hong Kong were physically and chemically characterized. They were chemically modified sludge from Stonecutters Island (CAPS) raw sludge from Tai Po and Yuen Long Sewage Treatment Plant (STP) (rTP & rYL) and anaerobically digested sludge from Tai Po and Yuen Long STP (dTP & dYL). It was found that CAPS sludge was better than other 4 sludge samples in terms of settleability and dewaterability. CAPS sludge contained significant higher amounts (p&lt;0.01) of extractable compounds than other sludges (except NO3− for dTP, NH4+ and PO43− for dYL). The concentration of total N and P in CAPS sludge were significantly higher (p&lt;0.01) than other sludges (except dYL). The concentrations of total Cu, Pb, Ni, Cd, Cr and K in the CAPS sludge were also significantly higher (p&lt;0.01) than other sludge samples. Most of the metals (Cr, Pb, Cr and Zn) in CAPS sludge were associated with the organically-bounded phase. It is concluded that there are significant differences in both physical and chemical properties between the chemically modified sludge and biological treated sludges.


2021 ◽  
Author(s):  
Yanyan Fang

Abstract Microplastics (MPs) have been found in all environment matrices and have become an issue of concern worldwide. In this study, Baiyangdian Lake in Northern China was investigated for the presence of MPs (0.45 µm–5 mm) in sediment and at different water depths. MPs were found at 1,000–20,000 pieces/m3 (average 9,595) in water and at 400–2,200 pieces/kg (average 1,023) in sediment. Since the implementation of pollution abatement measures, visible MPs have been nearly eliminated; the MPs found in this study were mainly in the micrometer range, with no more than 3–5 pieces greater than 1 mm per sample. The main forms of MPs were fibrous and fragmented, and the main components were polyamide, polyethylene, and polypropylene. MPs found in water near a garbage transfer station showed the following abundance of MPs: surface water < middle water < bottom water. The sediment contained a higher amount of MP fragments, indicating that the historical transfer and disposal of garbage was a main source of plastic deposition in this area. There was a high content of fibrous MPs in surface water, while the abundance of fragmented MPs increased with the depth of water. The main sources of MPs in the study area were residential activities, local plastic factories, and the treated effluent from a sewage treatment plant.


2017 ◽  
Vol 43 (4) ◽  
pp. 58-65 ◽  
Author(s):  
Marta Małecka-Adamowicz ◽  
Łukasz Kubera ◽  
Wojciech Donderski ◽  
Katarzyna Kolet

AbstractThe study was aimed at evaluating microbial contamination on the premises of the sewage treatment plant by determining the concentrations of selected groups of airborne microorganisms. Another objective was to determine the antibiotic sensitivity patterns of isolated strains of staphylococci. The research was conducted in a seasonal cycle, by the impaction method using Merck MAS-100 air sampler. Samples were collected at six sites, each representing a different stage of sewage treatment. The susceptibility of isolated staphylococci was assessed with the disc-diffusion method, following the recommendations of the EUCAST. The results indicate that the microbial population in the air of the investigated area was dominated by mold fungi, whose highest average concentration was recorded at site IV located near the final clarifier (7672 CFU•m-3). Heterotrophic bacteria and mannitol-positive staphylococci were the most numerous at locations where sewage undergoes primary treatment. In each subseuqent stage the number of microorganisms emitted into the air from the sewage was lower. Antibiograms show that more than 50% of Staphylococcus spp. exhibited resistance to penicillin and 20% to rifampicin. In addition, 90% of the analyzed strains were sensitive to other antibiotics. The fungal community included the following genera: Cladosporium, Fusarium, Alternaria, Penicillium, Aspergillus, Aureobasidium, and Acremonium.The highest air contamination with all studied groups of microorganisms was recorded at the locations where mechanical sewage treatment was performed. During the subsequent stages lower numbers of heterotrophic bacteria were emitted into the air. The air in the investigated sewage treatment plant did not contain multidrug-resistant staphylococci.


Irriga ◽  
2021 ◽  
Vol 1 (4) ◽  
pp. 671-677
Author(s):  
LIBIANE MARINHO BERNARDINO ◽  
PATRÍCIA DA SILVA COSTA ◽  
VERA LÚCIA ANTUNES DE LIMA ◽  
RENER LUCIANO DE SOUZA FERRAZ

POTENCIAL DE REÚSO DE EFLUENTES TRATADOS PARA IRRIGAÇÃO PERIURBANA NO MUNICÍPIO DE GUARABIRA/PB     LIBIANE MARINHO BERNARDINO1; PATRÍCIA DA SILVA COSTA2; VERA LÚCIA ANTUNES DE LIMA3 E RENER LUCIANO DE SOUZA FERRAZ4.   1 Mestranda em Gestão e Regulação em Recursos Hídricos, Unidade Acadêmica de Tecnologia do Desenvolvimento, UFCG, Rua Luiz Grande, s/n, Frei Damião, CEP 58540-000, Sumé, PB, Brasil, [email protected] 2 Doutoranda em Engenharia Agrícola, Unidade Acadêmica de Engenharia Agrícola, UFCG, Rua Aprigio Veloso, 882, Universitário, CEP 58429-900, Campina Grande, PB, Brasil, [email protected] 3 Profa. Doutora em Engenharia Agrícola, Unidade Acadêmica de Engenharia Agrícola, UFCG, Rua Aprigio Veloso, 882, Universitário, CEP 58429-900, Campina Grande, PB, Brasil, [email protected] 4 Prof. Doutor em Engenharia Agrícola, Unidade Acadêmica de Desenvolvimento Sustentável do Semiárido, UFCG, Rua Luiz Grande, s/n, Frei Damião, CEP 58540-000, Sumé, PB, Brasil, [email protected]     1 RESUMO   A água é um recurso finito que se encontra escasso, o que justifica a busca por gestão e inovação de práticas que a preserve. O objetivo da pesquisa é avaliar o potencial de reúso dos efluentes tratados para irrigação periurbana. Foram levantados dados de uma Estação de Tratamento de Esgotos (ETE), localizada no município de Guarabira, PB, e operada pela Companhia de Água e Esgotos do Estado da Paraíba (CAGEPA), durante o período de janeiro a dezembro de 2019. Analisou-se os seguintes parâmetros físico-químicos e bacteriológicos: Potencial Hidrogeniônico (pH), Condutividade Elétrica (CE), Demanda Bioquímica de Oxigênio (DBO), Demanda Química de Oxigênio (DQO), Sólidos Totais (ST), Oxigênio Dissolvido (OD), Fósforo Total, e Coliformes Termotolerantes. Os dados foram submetidos à análise descritiva e expresso em valores mínimos, máximos e médios. Os indicadores foram satisfatórios para irrigação restrita, porém com a necessidade de tratamento complementar para determinados cultivos. O potencial de reúso   dos efluentes tratados na ETE pode beneficiar uma área de 118,7 ha considerando uma demanda de irrigação de 18.000 m³ ha-1 ano-1, o que demonstra ser um recurso sustentável e que precisa ser regulamentado no Brasil.   Palavras-chave: recursos hídricos, resíduos líquidos, tratamento de água, fertirrigação.     BERNARDINO, L. M.; COSTA, P. S.; LIMA, V. L. A.; FERRAZ, L. R. S. REUSE POTENTIAL OF TREATED EFFLUENTS FOR PERIURBAN IRRIGATION IN THE MUNICIPALITY OF GUARABIRA/PB     2 ABSTRACT   Water is at the center of sustainable development and a finite resource that is in short supply, which justifies the search for management and innovation of practices that preserve it. This research aims to evaluate the potential for reuse of treated effluent for periurban irrigation. Data were collected from a Sewage Treatment Plant (STP), located in the municipality of Guarabira, PB, and operated by the Water and Sewage Company of the State of Paraíba (CAGEPA), during the period from January to December 2019, with the analysis of the following physicochemical and bacteriological parameters: Hipogenic Potential (pH), Electrical Conductivity (CE), Biochemical Oxygen Demand (DBO), Chemical Oxygen Demand (DQO), Total Solids (ST), Dissolved Oxygen (OD), Total Phosphorus, and Thermotolerant Coliforms. The data were submitted to the descriptive analysis and expressed as minimum, maximum and average values. The indicators were satisfactory for restricted irrigation, but with the need for complementary treatment for certain crops. The potential for reuse of the effluents treated in the ETE can benefit an area of 118.7 ha considering an irrigation demand of 18,000 m³ ha-1 year-1, which demonstrates to be a sustainable resource that needs to be regulated in Brazil.   Keywords: Water resources, liquid waste, water treatment, fertigation.


PeerJ ◽  
2018 ◽  
Vol 6 ◽  
pp. e5368 ◽  
Author(s):  
Anna Cristina P. Lima ◽  
Magali C. Cammarota ◽  
Melissa L.E. Gutarra

A residue from the primary treatment of a Wastewater Treatment Plant (WWTP) was used to isolate filamentous fungi with lipase production potential. Two of the 27 isolated fungi presented high hydrolysis index and were selected for lipase production by solid-state fermentation (SSF). The fermentations were conducted at 30 °C for 48 h, with moist air circulation, using 20% (w/w) of the residue mixture with a basal medium (agroindustrial residue—babassu cake), obtaining a solid enzymatic preparation (SEP) with lipase activity of 19 U/g with the fungus identified as Aspergillus terreus. Scum, collected in an anaerobic reactor operating in a WWTP, was hydrolyzed with SEP and subjected to anaerobic biodegradability tests at 30 °C. Different dilutions of crude (Control) or hydrolyzed scum in raw sewage were evaluated. The dilution of 5% (v/v) of hydrolyzed scum in raw sewage proved the most adequate, as it resulted in higher methane yield compared to the raw sewage (196 and 133 mL CH4/g CODadded, respectively), without increasing the chemical oxygen demand (COD) of the treated sewage (138 and 134 mg/L). The enzymatic hydrolysis of the scum, followed by dilution in the influent sewage, is technically feasible and increases methane production in anaerobic reactors.


2004 ◽  
Vol 50 (12) ◽  
pp. 79-86 ◽  
Author(s):  
D. Marani ◽  
V. Renzi ◽  
R. Ramadori ◽  
C.M. Braguglia

The objective of this work was to determine the partitioning of the pollutant load in urban wastewater in order to improve the conventional sewage treatment. In addition to settling tests, physical fractionation of COD in the degritted influent of Roma-Nord sewage treatment plant was performed via sequential filtration through sieves and membrane filters of the following pore size: 150-100-50-25-1-0.2 μm, and 100 kD (about 0.02 μm). Biodegradability studies were also performed on the different size fractions. Size fractionation showed that COD in Roma-Nord sewage is predominantly associated with settleable and supracolloidal (&gt; 1 μm) particles, each size range including about 40% of total COD. Biodegradability tests indicated that the large fraction of COD associated with supracolloidal particles, which are not removed in the primary treatment, is characterised by slow degradability. This suggests that removal of these particles prior to biological treatment may greatly improve the overall treatment scheme. Preliminary pilot plant coagulation tests with lime at pH 9 showed that lime-enhanced primary treatment may increase COD removal efficiencies from typical 30-35% up to 65-70%, by inducing almost complete removal of the COD fraction associated with supracolloidal particles.


2020 ◽  
Vol 5 (2) ◽  
pp. 53-57
Author(s):  
Nandini Moondra ◽  
Namrata D Jariwala ◽  
Robin A Christian

Conventional domestic wastewater treatment in most developing countries is confined to secondary treatments, mainly focusing on solids and organics removal, which results in eutrophication when the effluents are discharged into receiving bodies. Thus, to resolve the issues associated with the conventional treatment system, in the present study, microalgae was introduced in the primary treated effluent collected from a sewage treatment plant to study the efficiency of the system in reducing eutrophication and other challenges of secondary treatment. Phycoremediation is an effective and eco-friendly treatment alternative that reduced the primary-treated effluent’s PO4-P, NH3-N and COD concentration to 97.89%, 98.81%, and 88.24%, respectively at the identical HRT practiced for secondary treatment. One-way ANOVA was also conducted to determine the effectiveness of the system statistically. The experimental and statistical analysis proved that microalgal treatment could resolve the challenges of conventional secondary treatments if adopted for domestic wastewater.


Author(s):  
Vinay Khewale

A sewage water treatment plant is necessary to receive and treat waste water (Domestic, Commercial, and Industrial). Its objective is to be convert harmful waste water to safe water environmentally and treated effluent and treated sludge suitable for reuse and disposal such as farm fertilizer. The characteristics of waste water have been performed followed by design of sewage treatment plant. The present study includes design of sewage treatment plant and analysis of waste water – PH value, Total Dissolved Solids (TDS), Total Suspended Solids (TSS), Biochemical Oxygen Demand (BOD), Chemical Oxygen Demand (COD), Total Solids (TS), Hardness, Chloride, Acidity, Oil, Fats and grease etc. The sample collection of waste water has been done in many times in a day to obtain an average value of major parameter. Followed by values of this parameter, calculations are done for designing the units of sewage treatment plant and layout is prepared for the same


Sign in / Sign up

Export Citation Format

Share Document