scholarly journals Spectrophotometric Method for the Determination of Paracetamol

1970 ◽  
Vol 24 ◽  
pp. 39-44 ◽  
Author(s):  
Buddha Ratna Shrestha ◽  
Raja Ram Pradhananga
Keyword(s):  
Standard Deviation ◽  
Spectrophotometric Method ◽  
Trace Amount ◽  
Pharmaceutical Preparation ◽  
Chemical Society ◽  
Molar Absorptivity ◽  
Coupling Agents ◽  
Clinical Samples ◽  
Relative Standard

Paracetamol with 1-napthol or resorcinol gave azodye and the concentration of paracetamol was investigated spectrophotometrically. The azodyes formed with both 1-napthol and resorcinol as coupling agents follow Lambert Beer's law in the range of 0 to 10 µgmL-1 of paracetamol. The molar absorptivity and Sandell's sensitivity for azodye coupled with 1-napthol were found to be 1.68×104 Lmol-1cm-1 and 9.0 ngmL-1cm-2, respectively. The molar absorptivity and Sandell's sensitivity for azodye coupled with resorcinol were found to be 2.86×104 Lmol-1cm-1 and 5.3 ngmL-1cm-2, respectively. Both coupling agents had been applied successfully in the analysis of paracetamol in pharmaceutical preparation. The relative standard deviation for all five samples ranged from 2.2-6.4% at 95% confidence. The percentage recoveries were found to range from 97.8 to 103.4. Both methods used in the present study may be applied to the determination of trace amount of paracetamol in different clinical samples. Keyword: Paracetamol; Spectrophotometric; 1-napthol; Resorcinol DOI: 10.3126/jncs.v24i0.2389 Journal of Nepal Chemical Society Vol. 24, 2009 Page: 39-44

scholarly journals Estimation of Furosemide in Pharmaceutical Preparation Samples

2020 ◽  
Vol 1 (1) ◽  
Keyword(s):  
Standard Deviation ◽  
Spectrophotometric Method ◽  
Pharmaceutical Preparation ◽  
Pharmaceutical Preparations ◽  
Pharmaceutical Formulations ◽  
Molar Absorptivity ◽  
Oral Solution ◽  
Average Recovery ◽  
Relative Standard

A simple, accurate, precise, rapid, economical and sensitive ultraviolet spectrophotometric method has been developed for the determination of Furosemide in pharmaceutical preparations, which shows maximum absorbance at 228 nm in. Beer’s law was obeyed in the range of 1 -10 μg/ ml, with molar absorptivity of 3.5×104 L.mol-1.cm-1, relative standard deviation of the method was less than 1.4%, and accuracy (average recovery %) was 100 ± 1.0. The method was successfully applied to the determination of Furosemide in some pharmaceutical formulations (tablets, Oral solution and injection) samples. The proposed method was validated by sensitivity and precision which proves suitability for the routine analysis of Furosemide in true samples.

scholarly journals Development and validation of spectrophotometric method for phenylephrine hydrochloride estimation in nasal drops formulations

2008 ◽  
Vol 27 (2) ◽  
pp. 149 ◽  
Author(s):  
Ivana Savić ◽  
Goran Nikolić ◽  
Vladimir Banković
Keyword(s):  
Standard Deviation ◽  
Sodium Hydroxide ◽  
Relative Standard Deviation ◽  
Spectrophotometric Method ◽  
Molar Absorptivity ◽  
Dosage Forms ◽  
Phenylephrine Hydrochloride ◽  
Relative Standard ◽  
Development And Validation

Simple, accurate and reproducible UV-spectrophotometric method was developed and validated for the estimation of phenylephrine hydrochloride in pharmaceutical nasal drops formulations. Phenylephrine hydrochloride was estimated at 291 nm in 1 mol⋅dm-3 sodium hydroxide (pH 13.5). Beer’s law was obeyed in the concentration range of 10–100 μg⋅cm−3 (r2 = 0.9990) in the sodium hydroxide medium. The apparent molar absorptivity was found to be 1.63×103 dm3⋅mol−1⋅cm−1. The method was tested and validated for various parameters according to the ICH (International Conference on Harmonization) guidelines. The detection and quantitation limits were found to be 0.892 and 2.969 μg⋅cm−3, respectively. The proposed method was successfully applied for the determination of phenylephrine hydrochloride in pharmaceutical nasal drops formulations. The results demonstrated that the procedure is accurate, precise and reproducible (relative standard deviation < 1 %), while being simple, cheap and less time consuming, and hence can be suitably applied for the estimation of phenylephrine hydrochloride in different dosage forms.

scholarly journals Spectrophotometric Method for Determination of Paraquat in Food and Biological Samples

1997 ◽  
Vol 80 (2) ◽  
pp. 388-391 ◽  
Author(s):  
Ritu Kesari ◽  
Manish Rai ◽  
Vinay Kumar Gupta
Keyword(s):  
Standard Deviation ◽  
Metal Ions ◽  
Relative Standard Deviation ◽  
Alkaline Medium ◽  
Spectrophotometric Method ◽  
Biological Samples ◽  
Molar Absorptivity ◽  
Relative Standard ◽  
Sensitive Spectrophotometric Method

Abstract A sensitive spectrophotometric method was developed for determination of paraquat, a widely used herbicide. Paraquat was reduced with glucose in an alkaline medium, and the blue radical ion obtained was measured at 600 nm. Beer’s law was obeyed at 0.1–1.2 ppm paraquat. The molar absorptivity was 1.26 × 105 L mol-1 cm-1. The standard deviation and relative standard deviation were ± 0.007 and 2.0%, respectively, for 5 μg paraquat/10 mL analyzed over 7 days. The method was free from interference by other commonly used pesticides and metal ions. The method may be used to the determine paraquat in plants, fruits, grains, water, blood, and urine.

scholarly journals Flotation-Spectrophotometric Method for the Determination of Aluminium ion Using Xylenol Orange

2011 ◽  
Vol 8 (4) ◽  
pp. 1528-1535 ◽  
Author(s):  
F. Nekouei ◽  
Sh. Nekouei
Keyword(s):  
Standard Deviation ◽  
Spectrophotometric Method ◽  
Calibration Curve ◽  
Limit Of Detection ◽  
Xylenol Orange ◽  
Molar Absorptivity ◽  
Relative Standard ◽  
Ion Associate ◽  
Sensitive Method

A simple, fast, reproducible and sensitive method for the flotation- spectrophotometric determination of Al3+is reported. The apparent molar absorptivity (ε) of the ion associate was determined to be 8.35×104L mol-1cm-1. The calibration curve was linear in the concentration range of 1.0-50 ng mL-1of Al3+with a correlation coefficient of 0.9997. The limit of detection (LOD) was 0.621 ng mL. The relative standard deviation (RSD) at 10 and 30 ng mL-1of aluminium were 1.580 and 2.410% (n=7) respectively. The method was applied for measuring the amount of aluminium in water samples.

scholarly journals SPECTROPHOTOMETRIC DETERMINATION OF CEFPIROME IN PHARMACEUTICAL PREPARATION

2020 ◽  
pp. 124-127
Author(s):  
DILIP M CHAFLE
Keyword(s):  
Standard Deviation ◽  
Spectrophotometric Method ◽  
Limit Of Detection ◽  
Pharmaceutical Preparation ◽  
Effect Of Temperature ◽  
Percentage Error ◽  
Ferric Nitrate ◽  
Red Color ◽  
Relative Standard

Objective: A simple, sensitive and precise visible spectrophotometric method has been proposed for the determination of cefpirome (CFM) in pure and oral injectable dosage form. Methods: A spectrophotometric method is based on the formation of stable red color product by oxidation of drugs by ferric nitrate and subsequent complexation with 1, 10 – phenanthroline with maximum absorption at 515 nm. Result: The red color complex was formed between Fe (II) and 1, 10 – phenanthroline after reduction of Fe (III) to Fe (II) in the presence of CFM drug. The phosphoric acid solution was used only for quenching the complex formation reaction. Several parameters such as the maximum wavelength of absorption, the volume of reagents, sequence of addition and effect of temperature and time of heating were optimized to achieve high sensitivity, stability and reproducible results. Under the optimum conditions, linear relationship with good correlation coefficient (0.994) was found over the concentration range from 0.20 to 6.00 μg/mL with a molar extinction coefficient 7.7813 × 104 L/mol/cm, limit of detection 0.2026 and limit of quantification 0.6141 μg/mL, respectively. Conclusion: The proposed method was evaluated statistically for linearity, accuracy, and precision in terms of standard deviation, percentage recovery, percentage error and relative standard deviation. The proposed method can be applied for the routine estimation of CFM in the laboratory.

Spectrophotometric Determination of Boron in Dates of Some Cultivars Grown in Saudi Arabia

1993 ◽  
Vol 76 (3) ◽  
pp. 601-603 ◽  
Author(s):  
A A Al-Warthan ◽  
S S Al-Showiman ◽  
S A Al-Tamrah ◽  
A A BaOsman
Keyword(s):  
Saudi Arabia ◽  
Standard Deviation ◽  
Relative Standard Deviation ◽  
Spectrophotometric Determination ◽  
Spectrophotometric Method ◽  
Molar Absorptivity ◽  
Relative Standard ◽  
Sensitive Spectrophotometric Method

Abstract The formation of a red complex between boron and the quinalizarin reagent was investigated and used as the basis for a simple and sensitive spectrophotometric method for boron in date cultivars. At 620 nm, the absorbance was linear (r= 0.999) over the 0.25-2.5 μg/mL concentration range. The molar absorptivity was found to be 2.23 x 103mol-1cm-1 and the relative standard deviation for 10 replicates (1.0 μg/mL) was 0.97%.

scholarly journals Spectrophotometric method for determination of ranitidine hydrochloride in bulk and in pharmaceutical preparation using ninhydrin

2020 ◽  
Vol 11 (4) ◽  
pp. 291-297
Author(s):  
Hutaf Mustafa Baker ◽  
Hussam Ahmad Alsaoud ◽  
Hamzeh Mohamad Abdel-Halim
Keyword(s):  
Spectrophotometric Method ◽  
Limit Of Detection ◽  
Pharmaceutical Preparation ◽  
Pharmaceutical Preparations ◽  
Molar Absorptivity ◽  
Limit Of Quantification ◽  
Ranitidine Hydrochloride ◽  
Relative Standard ◽  
Reproducible Method

A simple, sensitive and reproducible method for the determination of ranitidine hydrochloride in pharmaceutical preparations was investigated. This spectrophotometric method was based on the formation of a deep red color product with ninhydrin in basic media and the absorbance measured at λmax = 480 nm. The reaction occurs at 45 °C with pH = 10 having a contact time of 38 minutes. Under the optimum conditions, Beer’s Law is obeyed in the concentration range of 8.98×103 - 9.90×104 µg/L. The coefficient of correlation was found to be 0.999 for the obtained method with molar absorptivity of 3.05×103 L/mol.cm. The calculated Sandell’s sensitivity is 0.108 μg/cm2. The limit of detection and limit of quantification are 0.0997 and 0.3023 µg/mL, respectively. The low values of the percentage relative standard deviation and percentage relative error indicate the high precision and the good accuracy of the proposed method. The stoichiometry of the reaction is determined and found to be 1:4 (Ranitidine hydrochloride:Ninhydrin). The initial rate method confirmed that this reaction is first order one.

Spectrophotometric Method for Microdetermination of Nitrogen in Kjeldahl Digest

1989 ◽  
Vol 72 (6) ◽  
pp. 953-956 ◽  
Author(s):  
Muljibhai B Devani ◽  
Chamanlal J Shishoo ◽  
Shailesh A Shah ◽  
Bhanubhai N Suhagia
Keyword(s):  
Standard Deviation ◽  
Nitrogen Content ◽  
Spectrophotometric Method ◽  
Acidic Medium ◽  
Molar Absorptivity ◽  
Nitrogen Compounds ◽  
Relative Standard ◽  
Kjeldahl Method ◽  
Aoac Method

Abstract A new spectrophotometric method for the determination of nitrogen in Kjeldahl digest has been developed. The method is based on the reaction of ammonia with acetylacetone-formaldehyde reagent in aqueous medium to yield yellow 3,5-diacetyl- 1,4-dihydrolutidine with a characteristic absorption maxima at 412 nm. The effect of several experimental variables on the determination of nitrogen was studied. The method was suitable for determination of nitrogen in acidic medium without interference from the usual catalysts employed for the digestion in Kjeldahl method. Lambert-Beer’s law is obeyed in the concentration range of 0.5-6.0 ng nitrogen/mL in the reaction mixture. The molar absorptivity in terms of nitrogen was 1.4 X 103L mol-1 cm-1. The standard deviation and relative standard deviation were ±0.0447 and ±0.896% (n = 10), respectively. The method is simple, rapid, and precise. A variety of carbocyclic and heterocyclic nitrogen compounds have been analyzed for nitrogen content. The results are comparable with those obtained by AOAC method, 47.021.

scholarly journals A New Spectrophotometric Method for Hydrogen Sulphide Through Electrolytically Generated Mn(III) WithO-Tolidine

2008 ◽  
Vol 5 (1) ◽  
pp. 93-99 ◽  
Author(s):  
M. S. Suresha ◽  
Mansour S. Abdul Galil ◽  
Mahadevaiah ◽  
M. A. Sathish ◽  
M. S. Yogendra Kumar ◽  
...  
Keyword(s):  
Standard Deviation ◽  
Hydrogen Sulphide ◽  
Spectrophotometric Method ◽  
Water Samples ◽  
Molar Absorptivity ◽  
Official Method ◽  
Color Intensity ◽  
Orange Yellow ◽  
Relative Standard

A simple, sensitive and economical spectrophotometric method for the determination of hydrogen sulphide is developed. The method is based on a redox reaction in that Mn(III) generated electrolytically is taken in excess, which is oxidizing a known but a less quantity of hydrogen sulphide and the unreacted oxidant will oxidize furthero-tolidine to produce an orange yellow quinonediimine absorbing cation (λ max. 455 nm). Therefore, in principle, the decrease in color intensity of the absorbing system is proportional to the concentration of hydrogen sulphide. The stoichiometry between Mn(III) ando-tolidine and stability constant of the complex were determined by Job's continuous method, the corresponding values were found to be 2:1 and 1.42X105Lmol-1. The system was obeying Lambert-Beer's law in the range 0.2-1.4 μg mL-1of hydrogen sulphide. Molar absorptivity, correlation coefficient and Sandell's sensitivity values were also calculated and found to be 4.2062X103L mol-1cm-1, 0.999 and 0.0012 μg cm-2respectively. The method was employed for the determination of hydrogen sulphide in water samples. The results obtained were reproducible with acceptable standard deviation 0.01-0.068 and relative standard deviation, less than 3.21%. For a comparison, hydrogen sulphide present in water samples were also determined separately following the methylene blue official method. The results of the proposed method compare well with the official method.

scholarly journals Extractive Spectrophotometric Method for Determination of Molybdenum in Steels and Environmental Samples

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Rameshwar Dass ◽  
Jitander K. Kapoor ◽  
Sunita Gambhir
Keyword(s):  
Ascorbic Acid ◽  
Standard Deviation ◽  
Spectrophotometric Method ◽  
Environmental Samples ◽  
Room Temperature ◽  
Molar Absorptivity ◽  
Accuracy And Precision ◽  
Relative Standard ◽  
Improved Accuracy

An extractive spectrophotometric method has been developed for microdetermination of molybdenum. At room temperature Mo(VI) was reduced to Mo(V) in 2 M H2SO4using ascorbic acid as a reductant. The Mo(V) formed a yellow-coloured (1 : 2) complex with 6-chloro-3-hydroxy-2-(3′-hydroxyphenyl)-4-oxo-4H-1-benzopyran (CHHB). The complex was quantitatively extracted into toluene and absorbed maximum atλmax404 nm. Beer’s law was obeyed over the concentration range of 2.31 μg Mo ml−1with a molar absorptivity and Sandell's sensitivity of5.62×104 L mol−1 cm−1and 0.0016 μg Mo cm−2, respectively. The relative standard deviation was 0.0025 (in absorbance units) for 10 replicate determinations of 1 μg Mo ml−1. The method was free from the interference of large number of analytically important elements. Molybdenum has been determined satisfactorily in different technical, synthetic, and environmental samples with improved accuracy and precision.

Sign in / Sign up

Export Citation Format

Share Document