Medium Optimization for the Production of Antioxidants from Aspergillus candidus

1999 ◽  
Vol 62 (6) ◽  
pp. 657-661 ◽  
Author(s):  
GOW-CHIN YEN ◽  
YUNG-CHI CHANG
Keyword(s):  
Antioxidant Activity ◽  
Organic Nitrogen ◽  
Strong Influence ◽  
Medium Optimization ◽  
Inorganic Nitrogen ◽  
Nitrogen Sources ◽  
Activity Inhibition ◽  
Aspergillus Candidus ◽  
Better Than ◽  
Ethyl Acetate Extracts

The objective of this study was to optimize the factors for the production of antioxidant from Aspergillus candidus CCRC 31543. Extracts of broth filtrate had higher antioxidant activity (inhibition of peroxidation [IP] >98%) when sucrose or lactose was used as a carbon source. Sucrose in the medium also resulted in a higher yield of extracts. Ethyl acetate extracts had the highest yield and antioxidant activity compared with the other two solvents. For the production of antioxidant, inorganic nitrogen sources were found to be more suitable than organic nitrogen sources, and ammonium sulfate was better than sodium nitrate. Yeast extract had a strong influence on the yield of antioxidant extracts. Both mycelium and broth filtrate of A. candidus CCRC 31543 showed similar antioxidant activity (IP = 95%), and they also had similar extraction yields.

scholarly journals Factors influencing mycelial growth of Sclerotium rolfsii

2013 ◽  
Vol 1 ◽  
pp. 26-31 ◽  
Author(s):  
Imdramani Bhagat
Keyword(s):  
Mycelial Growth ◽  
Organic Nitrogen ◽  
Inorganic Nitrogen ◽  
Sclerotium Rolfsii ◽  
Nitrogen Sources ◽  
Maximum Growth ◽  
Optimum Conditions ◽  
Factors Influencing ◽  
The World ◽  
Better Than

Sclerotial blight of tea (Camellia sinensis L.) caused by Sclerotium rolfsii Sacc. is one of the destructive diseases in tea growing areas of the world. In the present investigation, an attempt was made to know the optimum conditions for the mycelial growth of S. rolfsii. Factors influencing mycelial growth of S. rolfsii were studied with special reference to their growth in different media, variable pH and variable sources of carbon (viz., 6 types) as well as organic (viz., 4 types) and inorganic (viz., 4 types) nitrogen sources. Maximum growth of pathogen occurred after 8 days of inoculation at pH 6. Dextrose was the most effective carbon source and yeast extract (organic source) was found most optimum for growth of S. rolfsii. Organic nitrogen sources were found to be better than inorganic nitrogen sources. DOI: http://dx.doi.org/10.3126/njbs.v1i0.7466 Nepalese Journal of Biosciences 1: 26-31 (2011)

Effects of Additional Stimulants on Phenol Degradation by a Halotolerant Rhodococcus sp. JDD1H

2012 ◽  
Vol 253-255 ◽  
pp. 914-920 ◽  
Author(s):  
Fang Yan Chen ◽  
Duo Duo Jiang ◽  
Yu Bin Tang
Keyword(s):  
Energy Source ◽  
Organic Nitrogen ◽  
High Salinity ◽  
Inorganic Nitrogen ◽  
Phenol Degradation ◽  
Nitrogen Sources ◽  
Tween 80 ◽  
Cane Sugar ◽  
Wide Range ◽  
Rhodococcus Sp

A halotolerant and highly effective phenol-degrading strain, named as JDD1H, was isolated from soil contaminated by petroleum and identified as Rhodococcus sp. Phenol degradation by strain JDD1H in the presence of high salinity, especially the effect of exotic stimulants such as carbon source, nitrogen source and surfactant on phenol degradation was investigated. The results show that strain JDD1H could utilize phenol as sole carbon and energy source to grow in the presence of 5% NaCl with 16 h adaptive phase. The degradation efficiency of 150 mg/L phenol was up to 99.5% within 40 h. Lactose, cane sugar, glucose and mannitol in wide range of 100~2000 mg/L enhanced phenol degradation significantly. Organic nitrogen sources, as peptone and yeast extract, and inorganic nitrogen sources, as NH4Cl and (NH4)2SO4, accelerated phenol degradation. Tween-80 with concentration of 50-1000 mg/L restrained phenol degradation and Tween-80 of 1500-2000 mg/L could promote degradation.

Interactions between nitrogen sources and xylose affecting growth, sporulation, and polyphenoloxidase activity in Bipolaris maydis race T

1981 ◽  
Vol 59 (11) ◽  
pp. 2102-2107 ◽  
Author(s):  
Robert C. Evans ◽  
Candace L. Black
Keyword(s):  
Amino Acids ◽  
Organic Nitrogen ◽  
Inorganic Nitrogen ◽  
Nitrogen Concentration ◽  
Relative Effectiveness ◽  
Nitrogen Sources ◽  
Dry Weight ◽  
The Other ◽  
Growth Media ◽  
Bipolaris Maydis

The effect of 23 organic and 3 inorganic nitrogen sources on growth, sporulation, and polyphenoloxidase activity was measured in Bipolaris maydis race T incubated with or without a xylose supplement. Mycelial dry weight tends to be highest on acidic and other polar amino acids and least on nonpolar ones; organic nitrogen sources generally result in greater dry weight than inorganic ones. Changes in nitrogen concentration and pH of media influence growth and sporulation, but only pH alters the relative effectiveness of the nitrogen sources on these processes. The addition of xylose to the growth media has little effect on growth. However, in the presence of lysine, serine, asparagine, glycine, γ-alanine, alanine, and α-aminobutyrate, xylose causes an increase in sporulation and a concomitant decrease in polyphenoloxidase activity. There is no consistent pattern resulting from xylose addition in the presence of the other nitrogen sources.

A NUTRITIONAL STUDY OF THREE STRAINS OF CLAVICEPS PURPUREA (FR). TUL.

1957 ◽  
Vol 3 (1) ◽  
pp. 1-12 ◽  
Author(s):  
W. A. Taber ◽  
L. C. Vining
Keyword(s):  
Organic Nitrogen ◽  
Inorganic Nitrogen ◽  
Methyl Cellulose ◽  
Nitrogen Sources ◽  
Tween 80 ◽  
Early Growth ◽  
Claviceps Purpurea ◽  
Nutritional Study ◽  
Ammonium Succinate ◽  
Exogenous Supply

Organic nitrogen sources were more readily utilized than inorganic nitrogen sources by three strains of Claviceps purpurea. Glucose, fructose, mannose, cellobiose, and sucrose were readily utilized, but not xylose, ribose, mannitol, maltose, starch, powdered cellulose, carboxymethyl cellulose, or methyl cellulose. Carbon dioxide was required for initiation of growth. An exogenous supply of biotin was required for growth in a medium containing glucose, ammonium succinate, and minerals. This requirement could be nearly replaced by equimolar amounts of biotin L-sulphoxide and biocytin. Desthiobiotin slightly replaced biotin but homobiotin, norbiotin, oleate, aspartate, pimelate, and Tween 80 were inactive. Oleate and aspartate stimulated early growth.

scholarly journals Effect of nitrogen supplementation on aerobic degradation of linear alkylbenzene sulfonate by consortia of bacteria

2012 ◽  
Vol 2 (1) ◽  
pp. 5
Author(s):  
Kehinde I. Temitope Eniola
Keyword(s):  
Organic Nitrogen ◽  
Linear Alkylbenzene Sulfonate ◽  
Micrococcus Luteus ◽  
Inorganic Nitrogen ◽  
Nitrogen Sources ◽  
Aerobic Degradation ◽  
Nitrogen Supplementation ◽  
Inorganic Nitrogen Source ◽  
Linear Alkylbenzene ◽  
Alkylbenzene Sulfonate

Untreated detergent bearing wastes discharged into the environment are sources of linear alkylbenzene sulfonate (LAS). Detergent wastes usually do not contain nitrogen or contain very low amounts. Biostimulation by introducing limiting nutrient element can be useful in biotreatment of such waste. The effect of inorganic and organic nitrogen supplements on aerobic degradation of LAS by LAS-utilizing bacteria was examined. Phosphate-buffered LAS mineral media were prepared and supplemented with different nitrogen sources: NPK fertilizer (inorganic) and urea fertilizer (organic). Individual and various consortia of pure cultures of <em>Alcaligenes odorans</em>, <em>Citrobacter diversus</em>, <em>Micrococcus luteus</em> and <em>Pseudomonas putida</em>, previously isolated from a detergent effluent polluted stream, were used. Biodegradation of LAS was monitored in terms of half-life (t&frac12;) of the surfactant. The rates of biodegradation by the consortia can be ranked as: 4-membered (t&frac12;=8-12 days) &gt;3-membered (t&frac12;=8-13 days) &gt;2-membered consortia (t&frac12;=10-15 days) &gt;individuals (t&frac12;=9-16 days). The inorganic nitrogen source enhanced utilization of the surfactant, while organic nitrogen supplementation generally slowed degradation of the surfactant. In undertaking biotreatment of detergent bearing effluent, inorganic nitrogen should be used as biostimulant.

Antioxidant Activity of Extracts from Molds

1996 ◽  
Vol 59 (12) ◽  
pp. 1327-1330 ◽  
Author(s):  
GOW-CHIN YEN ◽  
CHE-AN LEE
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Incubation Temperature ◽  
Maximum Level ◽  
Penicillium Oxalicum ◽  
Broth Culture ◽  
Aqueous Extracts ◽  
Aspergillus Sp ◽  
Aspergillus Candidus ◽  
Ethyl Acetate Extracts

The objective of this work was to screen molds for antioxidant activity. The results of screening 10 molds showed that three, Aspergillus candidus CCRC 31543, Aspergillus sp. CCRC 31742, and Penicillium oxalicum CCRC 31670, produce antioxidants that can be extracted from broth culture filtrates by ethyl acetate. The most antioxidant activity was found in the ethyl acetate extracts of A. candidus CCRC 31543. The antioxidant activity of A. candidus CCRC 31543 was as strong as that of rosemary extracts and butylated hydroxyanisole and was greater than that of α-tocopherol at the same concentrations. No antioxidant activity was found in aqueous extracts of A. candidus broth filtrate. A. candidus was cultured in a 500-ml Hiton flask containing 100 ml of growth broth, which consisted of 3% sucrose, 0.1 % yeast extract, 0.1 % polypeptone, 0.3% NaNO3, 0.1% K2HPO4, 0.05% MgSO4·· 7H2O, 0.05% KCl, and 0.001 % FeSO4 · 7H2O, at an incubation temperature of 25°C for 15 days, and a shaking rate of 130 rpm. The antioxidant activity of A. candidus reached a maximum level after 13 days of culture.

scholarly journals Effects of nitrogen and carbon sources on the production of inulinase from strain Bacillus sp. SG113

2016 ◽  
Vol 3 (1) ◽  
pp. 69-74
Author(s):  
Simeon Gavrailov ◽  
Viara Ivanova
Keyword(s):  
Nitrogen Source ◽  
Organic Nitrogen ◽  
Inorganic Nitrogen ◽  
Carbon Sources ◽  
Casein Hydrolysate ◽  
Jerusalem Artichoke ◽  
Nitrogen Sources ◽  
Cellular Growth ◽  
Bacillus Sp ◽  
Carbon And Nitrogen

Abstract The effects of the carbon and nitrogen substrates on the growth of Bacillus sp. SG113 strain were studied. The use of organic nitrogen sources (peptone, beef extract, yeast extract, casein) leads to rapid cellular growth and the best results for the Bacillus strain were obtained with casein hydrolysate. From the inorganic nitrogen sources studied, the (NH4) 2SO4 proved to be the best nitrogen source. Casein hydrolysate and (NH4) 2SO4 stimulated the invertase synthesis. In the presence of Jerusalem artichoke, onion and garlic extracts as carbon sources the strain synthesized from 6 to 10 times more inulinase.

Nitrogen requirements of the genus Linderina

1970 ◽  
Vol 48 (4) ◽  
pp. 695-698 ◽  
Author(s):  
R. C. Stephen ◽  
Christina Chan
Keyword(s):  
Glutamic Acid ◽  
Organic Nitrogen ◽  
Liquid Culture ◽  
Growth Response ◽  
Nitrate Nitrogen ◽  
Nitrogen Sources ◽  
Ammonium Nitrogen ◽  
Good Growth ◽  
Poor Growth ◽  
Better Than

The influence of different nitrogen sources on the growth of Linderina was examined in liquid culture. Both species of Linderina were unable to assimilate nitrate nitrogen and nitrite seemed to be toxic. Ammonium nitrogen was used but the growth response was considerably lower than that with some organic nitrogen materials. Inclusion in the growth medium of succinic acid as a carbon source failed to improve the assimilation of ammonium.Amino nitrogen as aspartic acid and asparagine gave good growth though not as good as with L- or DL-glutamic acid. The response to DL-glutamic acid was markedly better than to the L-isomer whereas the D-isomer gave relatively poor growth.

scholarly journals Pemanfaatan sumber nitrogen organik dalam pembuatan nata de coco

2021 ◽  
Vol 12 (1) ◽  
pp. 52-60
Author(s):  
Budi Santosa ◽  
Rozana Rozana ◽  
Astutik Astutik
Keyword(s):  
Nitrogen Source ◽  
Organic Nitrogen ◽  
Extracellular Enzymes ◽  
Inorganic Nitrogen ◽  
Liquid Waste ◽  
Nitrogen Sources ◽  
Acetobacter Xylinum ◽  
Crude Fiber ◽  
Azolla Microphylla ◽  
Crude Fiber Content

Nata de coco is made from raw coconut water which is fermented using the Acetobacter xylinum bacteria. This product has the characteristics of a white color, a thickness of approximately 1-2 cm and a chewy texture like a gel. The largest content in nata de coco is fiber known as bacterial cellulose. In addition to requiring sugar as a carbon source, making nata de coco also requires a source of nitrogen to activate the extracellular enzymes of the Acetobacter xylinum bacteria in the manufacture of nata cellulose. Sources of nitogen used in the manufacture of nata de coco generally use inorganic nitrogen sources, which in their development have caused many pros and cons, especially with regard to food safety issues when this product is consumed as a beverage. This study aims to obtain the best alternative nitrogen source and concentration in making nata de coco. The experimental design used in this study was nested randomized design with 2 factors as the first factor, the type of nitrogen source, while the second factor was the concentration of the nitrogen source in the first factor. The types of nitrogen sources used as the first factor were tofu industrial wastewater, green bean sprouts and Azolla microphylla, while the second factor was the concentration of nitrogen sources consisting of 4 levels, namely 0.5%, 1%, 1.5% and 2%. The parameters observed in this study were thickness of nata de coco, weight of nata de coco, crude fiber of nata de coco, number of Acetobacter xylinum cells in the nata de coco layer. The results showed that organic nitrogen sources (tofu, sprouts and Azolla microphylla liquid waste can be an alternative substitute for inorganic nitrogen sources (urea, ZA and ammonium sulfate) which have been commonly used in making nata. The highest quality nata de coco is produced from the treatment of organic nitrogen sources. sprouts with a concentration of 1.5% with a thickness of 2.83 cm, a weight of 279.33 grams, a crude fiber content of 4.14% and the number of Acetobacter xylinum cells in the cellulose layer 0.4 x 107 cells / ml.

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