The indices enzyme activity of the antioxidant system and content of malonic dialdehyde and diene conjugates in the blood and lacrimal fluid experimental uveitis

Modern environmental conditions dramatically increased the level of radical forming processes in the body. Heat exposure stimulates the generation of reactive oxygen species inducing peroxidation of lipids as a result of the development of hypoxia. In experimental conditions the possibility to correct free radical lipid oxidation of rats’ organism membranes was studied with the oral introduction of the phytoadaptogens that contain the complex of natural antioxidants. The animals were divided into 5 groups and each of them had 30 rats: intact animals (1) which were held in standard conditions of vivarium; the control group (2) in which rats were exposed to heat during forty-five minutes daily; the experimental groups (3, 4, 5) in which before the effects of heat animals had a daily oral intake of the extracts of Eleutherococcus, Rhodiola, liquorice in a dose of 1 ml/kg. It was found out that in the liver tissue of experimental animals a daily heat exposure during forty-five minutes contributes to the increase of lipid hydroperoxides level (by 34-41%), of diene conjugate (by 45-50%), and of malonic dialdehyde (by 62-74%) against the decrease of antioxidant system activity in the liver of intact animals. The introduction of phytoadaptogens to rats in the conditions of heat exposure contributes to the reliable decrease in the liver of lipid hydroperoxides by 22-28%, of diene conjugates by 22-32%, and of malonic dialdehyde by 30-37% in comparison with the rats of the control group. While analyzing the effect of the phytoadaptogens on the activity of the components of antioxidant system it was shown that the level of ceruloplasmin in the liver of animals was reliably higher by 68-82%, of vitamin E by 42-65% in comparison with the same parameters of the rats of the control group. So, the application of phytoadaptogens in the conditions of long heat exposure of the organism of animals under experiment leads to the stabilization of the processes of peroxidation against the increase of antioxidant system activity.

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COMPARATIVE EFFECTIVENESS OF SYNTHETIC AND NATURAL ANTIOXIDANTS IN TOXIC LIVER DAMAGE BY CARBON TETRACHLORIDE

Bulletin physiology and pathology of respiration ◽

10.12737/article_5a9f269bb59ec6.60222914 ◽

2018 ◽

Vol 1 (67) ◽

pp. 64-69 ◽

Cited By ~ 1

Author(s):

Наталья Симонова ◽

Natalya Simonova ◽

Владимир Доровских ◽

Vladimir Dorovskikh ◽

Михаил Штарберг ◽

...

Keyword(s):

Carbon Tetrachloride ◽

Antioxidant System ◽

Liver Damage ◽

Malonic Dialdehyde ◽

Natural Antioxidants ◽

Control Group ◽

Lipid Hydroperoxides ◽

Diene Conjugates ◽

Toxic Liver Damage ◽

Experimental Group

Currently there is an active search for medicines increasing the stability of liver to pathological effects, reinforcing its neutralizing function, promoting its functional recovery after various injuries, including poisoning by industrial poisons. Therefore, the study of opportunities to reduce toxic liver damage by carbon tetrachloride introduction of synthetic and natural antioxidants is of interest. In experimental conditions the possibility to correct free radical lipid oxidation of rats’ organism membranes was studied with the introduction of the succinate containing drug called Reamberin (Polysan, St.Petersburg) and licorice syrup. The animals were divided into 4 groups and each of them had 10 rats: the group with intact animals which were held in standard conditions of vivarium; the control group in which rats were given carbon tetrachloride during 3 days daily; the experimental group in which before the introduction of carbon tetrachloride animals had a daily intra-abdominal intake of the Reamberin in a dose of 100 mg/kg (20 ml/kg); the experimental group in which before the introduction of carbon tetrachloride animals had a daily oral intake of the licorice syrup in a dose of 5 ml/kg. It was found out that in the blood and in the liver of experimental animals an introduction of carbon tetrachloride during 3 days contributes to the increase of lipid hydroperoxides level (by 24-43%), of diene conjugate (by 19-47%), and of malonic dialdehyde (by 61-81%) against the decrease of antioxidant system activity in the blood of intact animals. The introduction of the succinate containing drug to rats in the conditions of oxidative stress contributes to the decrease in the blood and in the liver of lipid hydroperoxides by 13-26%, of diene conjugates by 11-28%, and of malonic dialdehyde by 30-34% in comparison with the rats of the control group. Adding of the licorice syrup to rats contributes to the reliable decrease in the blood and in the liver of lipid hydroperoxides by 6-23%, of diene conjugates by 9-24%, and of malonic dialdehyde by 25-30% in comparison with the rats of the control group. While analyzing the effect of the antioxidants on the activity of the components of antioxidant system it was shown that the level of ceruloplasmin in the blood and in the liver of animals was reliably higher by 10-47%, of vitamin E by 13-42% in comparison with the same parameters of the rats of the control group. So, the application of the synthetic and natural antioxidants in the conditions of introduction of carbon tetrachloride of the organism of animals under experiment leads to the stabilization of the processes of peroxidation against the increase of antioxidant system activity.

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COMPARATIVE EFFECTIVENESS OF SUCCINIC ACID AND REAMBERIN IN THE OXIDATIVE STRESS IN EXPERIMENT

Bulletin physiology and pathology of respiration ◽

10.12737/article_5c126def73b749.24896834 ◽

2018 ◽

Vol 1 (70) ◽

pp. 78-82 ◽

Cited By ~ 1

Author(s):

Наталья Симонова ◽

Natalya Simonova ◽

Владимир Доровских ◽

Vladimir Dorovskikh ◽

А Кропотов ◽

...

Keyword(s):

Oxidative Stress ◽

Ultraviolet Radiation ◽

Succinic Acid ◽

Antioxidant System ◽

Malonic Dialdehyde ◽

Control Group ◽

Lipid Hydroperoxides ◽

Diene Conjugates ◽

System Activity ◽

Experimental Group

The search and development of methods for correction of oxidative stress in conditions of exposure to adverse environmental factors is an actual problem of modern medicine. In experimental conditions the possibility to correct free radical lipid oxidation of rats’ organism membranes was studied with the introduction of the succinic acid and of the succinate containing medication called Reamberin (Polysan, St. Petersburg). The animals were divided into 4 groups and each of them had 20 rats: the group with intact animals which were held in standard conditions of vivarium; the control group in which rats were exposed to ultraviolet radiation during three minutes daily; the experimental group in which before ultraviolet radiation animals had a daily intraabdominal intake of the succinic acid in a dose of 100 mg/kg; the experimental group in which before ultraviolet radiation animals had a daily intraabdominal intake of Reamberin in a dose of 100 mg/kg (20 ml/kg). It was found out that in the blood of experimental animals a daily ultraviolet radiation during three minutes contributes to the increase of lipid hydroperoxides level (by 48-53%), of diene conjugate (by 43-48%), and of malonic dialdehyde (by 48-61%) against the decrease of antioxidant system activity in the blood of intact animals. The introduction of the succinic acid to rats contributes to the significant decrease in the blood of lipid hydroperoxides by 15-16%, of diene conjugates by 9-16%, and of malonic dialdehyde by 15% in comparison with the rats of the control group. The introduction of the succinate containing Reamberin to rats in the conditions of oxidative stress contributes to the significant decrease of lipid hydroperoxides in the blood by 27-28%, of diene conjugates by 23-28%, and of malonic dialdehyde by 26-29% in comparison with the rats of the control group. While analyzing the effect of the succinate containing drugs on the activity of the components of antioxidant system it was shown that the level of ceruloplasmin in the blood of animals was significantly higher by 25-32%, of vitamin E by 28-33% in comparison with the rats of the control group. So, the application of the succinate containing antioxidants in the conditions of ultraviolet radiation under experiment leads to the stabilization of the processes of peroxidation against the increase of antioxidant system activity. The intraabdominal introduction in laboratory animals of Reamberin in a dose of 100 mg/kg of succinate prevents the accumulation of lipoperoxidation products and increases the activity of main components of the antioxidant system in rats’ blood plasma, which indirectly exceeds similar effect of succinic acid in a dose of 100 mg/kg in the conditions of oxidative stress.

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Clinical experience of evaluation of intoxication level and endogenous antioxidant system dynamics in patients with acute peritonitis

Medical and Clinical Chemistry ◽

10.11603/mcch.2410-681x.2015.v17.i3.5062 ◽

2015 ◽

Vol 17 (3) ◽

Author(s):

B. O. Mihenko

Keyword(s):

Enzyme Activity ◽

Glutathione Peroxidase ◽

System Dynamics ◽

Glutathione Reductase ◽

Clinical Experience ◽

Antioxidant System ◽

Diene Conjugates ◽

Acute Peritonitis ◽

Endogenous Antioxidant ◽

Glucose 6 Phosphate Dehydrogenase

The results of 123 patients with acute peritonitis were analyzed. All patients were operated in toxic and terminal phases. The indicators of intoxication and enzyme activity of antioxidant system were estimated within 6 hours, 1 and 3 days after surgery treatment. As result of this investigation was confirmation that one of the factors homeostasis violations in peritonitis is activation of peroxide lipid – diene conjugates, TBA-active products in the blood and reduce the activity of antioxidant system – glutathione peroxidase, catalase, glucose-6-phosphate dehydrogenase and glutathione reductase as main branch of antiradikal and antiperoxidative protection.

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Assessment of the state of lipid peroxidation and antioxidant system in children with nephrotic form of glomerulonephritis

Kazan medical journal ◽

10.17750/kmj2017-370 ◽

2017 ◽

Vol 98 (3) ◽

pp. 370-375

Author(s):

R O Beglyarov

Keyword(s):

Lipid Peroxidation ◽

Nephrotic Syndrome ◽

Blood Plasma ◽

Antioxidant System ◽

Malonic Dialdehyde ◽

Control Group ◽

Chronic Glomerulonephritis ◽

Diene Conjugates ◽

Control Value ◽

In Blood Plasma

Aim. Study of the activity of lipid peroxidation and state of antioxidant system in children with a nephrotic form of chronic glomerulonephritis. Methods. 104 children with a nephrotic form of glomerulonephritis were examined. The average age of children was 10.18±4.03 years. 46.2% of children were in remission, 32.7% of children had 1st degree of activity, 14.4% had 2nd degree, and 6.7% had 3d degree of nephrotic syndrome. Control group included 30 children without chronic glomerulonephritis. The levels of diene conjugates, malonic dialdehyde, catalase, reduced glutathione, glutathione peroxidase, and glutathione reductase in blood plasma and erythrocytes were determined. Results. In children with chronic glomerulonephritis the concentration of diene conjugates and malonic dialdehyde was elevated in blood plasma and erythrocytes. In erythrocytes their concentration was statistically significantly higher at all degrees of activity of nephrotic syndrome than in control group. The level of catalase in patients’ blood plasma in comparison with the control value was reduced by 16.6%, in erythrocyte mass - by 25.9% (p

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Зміни функціонального стану прооксидантної та антиоксидантної систем у надниркових залозах морських свинок за умов формування експериментального алергічного альвеоліту

Medical and Clinical Chemistry ◽

10.11603/mcch.2410-681x.2017.v0.i4.8304 ◽

2018 ◽

Author(s):

M. Ye. Kovalska

Keyword(s):

Lipid Peroxidation ◽

Antioxidant System ◽

Cell Damage ◽

Malonic Dialdehyde ◽

Statistical Processing ◽

The State ◽

Antioxidant Systems ◽

Allergic Alveolitis ◽

Diene Conjugates ◽

Experimental Allergic

Introduction. Important role in understanding the pathogenesis of allergic alveolitis is played by processes of lipid peroxidation (LPO) and antioxidant system (AOS). Under physiological conditions, the level of LPO is maintained due to the equilibrium of pro- and antioxidant systems. LPO is considered as a universal mechanism of cell damage at the membrane level in conditions of different pathological conditions. In particular, hypoxia, inflammation, stress, allergy activate lipoperoxidation processes and suppress both an enzymatic and non-enzymatic activity of antioxidant defense. Products of lipoperoxidation negatively affect cell membranes and have a direct damaging effect on the adrenal gland tissue.The aim of the study – to evaluate the influence of lipid peroxidation processes and the state of the antioxidant system in the adrenal glands in the conditions of the formation of experimental allergic alveolitis.Research Methods. The experiments were conducted on 36 guinea pigs, body weight 0.18-0.20 kg. They were divided into four groups (n = 9). Intact – the first group (control). Animals with experimental allergic alveolitis, respectively, on the 1st, 2nd, and 34th day of the experiment. Experimental allergic alveolitis was modeled by O. O. Oriekhov, Yu. A. Kyrylov method. Activity of catalase was studied by B.Holmes, C. Masters method; superoxide dismutase – by R. Fried method; diene conjugates – by V. B. Havrylov, M. I. Mishkorudna method; malonic dialdehyde – by E. N. Korobeinikov method. Statistical processing is standard.Results and Discussion. The results of our studies revealed that the activity of the antioxidant system enzymes varied unidirectionally, depending on the periods of formation of the experimental allergic alveolitis. There is an increase in lipid peroxidation products both in its initial and final stages. At the same time, the activity of individual enzymes of antioxidant system underwent opposite changes.Conclusion. The obtained results indicate significant changes in the state of pro- and antioxidant systems in the thymus of animals with experimental allergic alveolitis and are important for a better understanding of the pathogenesis of experimental allergic alveolitis. These studies provide an opportunity to find more effective and useful ways to correct the detected changes in experimental allergic alveolitis.

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Research of enzyme activity changes in the antioxidant system and the state of NO-system in patients with colon cancer

Annales UMCS Pharmacia ◽

10.2478/v10080-008-0128-4 ◽

2008 ◽

Vol 21 (2) ◽

pp. 255-258

Author(s):

Sophia Kovalchuc ◽

Andriy Revura ◽

Bohdan Melekh ◽

Oksana Khavrona ◽

Yuriy Fedevych ◽

...

Keyword(s):

Colon Cancer ◽

Enzyme Activity ◽

Antioxidant System ◽

The State

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Features of ozone effect on oxygen-dependent processes in the blood under hypoxic conditions

Regional blood circulation and microcirculation ◽

10.24884/1682-6655-2021-20-3-70-76 ◽

2021 ◽

Vol 20 (3) ◽

pp. 70-76

Author(s):

V. V. Zinchuk ◽

E. S. Biletskaya

Keyword(s):

Malonic Dialdehyde ◽

Oxygen Affinity ◽

Diene Conjugates ◽

Physiological Factor ◽

Sodium Hydrosulfide ◽

Hypoxic Conditions ◽

Hemoglobin Oxygen Affinity ◽

Gaseous Transmitters ◽

Dependent Processes

Introduction. Ozone is a physiological factor that can change hemoglobin oxygen affinity and the formation of gaseous transmitters (NO, H2S). The aim is to study the effect of ozone with gaseous transmitters donors on oxygen-dependent processes in the blood under hypoxic conditions in vitro. Materials and methods. Blood samples were divided into 6 groups of 3 ml each. Groups 2, 4, 5, 6 were pretreated with a deoxygenating gas mixture (5.5 % CO2; 94.5 % N2). In groups 3, 4, 5, 6, ozonized isotonic sodium chloride solution (with an ozone concentration of 6 mg/l) was added, and in groups 5 and 6, the donors of gas transmitters nitroglycerin and sodium hydrosulfide, respectively, were additionally introduced. Results. Pre-deoxygenation reduces the effect of ozone on oxygen transport in the blood. Nitroglycerin prevents this effect. The action of ozone under hypoxic conditions leads to an increase of content of NO3-/NO2- and H2S, and combination with nitroglycerin and sodium hydrosulfide increase these parameters. Deoxygenation due to ozone reduces parameters of lipid peroxidation (malonic dialdehyde, diene conjugates), retinol and α-tocopherol, and the same result in the nitroglycerin group. Conclusion. Under hypoxic conditions, a decrease in the effect of ozone on oxygen-dependent processes is reported. Nitroglycerin reduces its manifestation, while sodium hydrosulfide does not have a similar effect.

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Analysis of the relationship between hemoglobin-oxygen affinity and lipid peroxidation during fever.

Acta Biochimica Polonica ◽

10.18388/abp.1995_4670 ◽

1995 ◽

Vol 42 (1) ◽

pp. 69-74 ◽

Cited By ~ 6

Author(s):

M V Borisiuk ◽

V V Zinchuk

Keyword(s):

Lipid Peroxidation ◽

Malonic Dialdehyde ◽

Oxygen Affinity ◽

Alpha Tocopherol ◽

Diene Conjugates ◽

Tocopherol Concentration ◽

Hemoglobin Oxygen Affinity ◽

The Relationship ◽

Free Radical Lipid Oxidation ◽

Oxyhemoglobin Dissociation

Endogenous hyperthermia was induced in rabbits by i.v. pyrogenal administration. Hemoglobin-oxygen affinity and parameters of free radical lipid oxidation in plasma and red blood cells were measured. The content of diene conjugates, malonic dialdehyde and Schiff bases were determined at a pyrogenal dose of 4 minimal pyrogenic doses/kg, and iron-initiated chemiluminescence, catalase activity and alpha-tocopherol concentration were determined at 6 minimal pyrogenic doses/kg. A rightward shift of the real oxyhemoglobin dissociation curve and activation of lipid peroxidation were observed. Relationships between the parameters measured were analyzed. Decreased hemoglobin-oxygen affinity is considered to be a possible mechanism of activation of free radicals during fever.

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Features of therapy of alcoholic liver disease in persons of young age

Experimental and Clinical Gastroenterology ◽

10.31146/1682-8658-ecg-174-2-80-85 ◽

2020 ◽

Vol 174 (2) ◽

pp. 80-85

Author(s):

R. G. Myazin ◽

D. N. Emel’yanov

Keyword(s):

Lipid Peroxidation ◽

Liver Disease ◽

Glutathione Peroxidase ◽

Antioxidant System ◽

Alcoholic Liver Disease ◽

Laboratory Data ◽

Young Patients ◽

Well Being ◽

Diene Conjugates ◽

Treatment Regimens

The aim of this study was to study the eff ects of using the infusion drug Remaxol in young patients with alcoholic liver disease.Materials and methods: the clinical and laboratory data of 80 young patients with alcoholic liver disease were analyzed, divided depending on the treatment regimen into 2 groups: the main (n = 44) who received Remaxol according to the scheme: 400 ml iv, drip, at a rate 40–60 drops per minute daily, course No. 5–10, and control (n = 36) — received basic therapy. In all patients, the dynamics in blood serum was studied: lipid peroxidation indices (malondialdehyde, diene conjugates), antioxidant protection enzymes (catalase, SOD, glutathione peroxidase), markers of cytolysis, cholestasis and lipid metabolism.Results: initially there was a significant increase in lipid peroxidation processes, accompanied by slight stimulation of the antioxidant system of the liver and pronounced signs of cytolysis and cholestasis. The inclusion of Remaxol in treatment regimens leads to a significant decrease in the level of malondialdehyde and diene conjugates, and the restoration of the antioxidant system (increase in catalase, SOD, and glutathione peroxidase). At the same time, a marked decrease in the syndromes of cytolysis and cholestasis was noted, which was accompanied by an improvement in the condition of the patients: cessation of nausea, restoration of appetite and sleep, and improvement of well-being.

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