Effect of 20-Hydroxyecdysone and S-adenosylmethionine on the ecdysone receptor gene EcR transcriptional activity in housefly Musca domestica L.

Steroid hormone 20-hydroxyecdysone (20E) initiates larval molting start and metamorphosis and regulates reproduction. Its basic receptor is heterodimer including proteins EcR and USP. Ecdysone receptor gene EcR coding protein EcR is a key regulatory element of gene circuits cover considerable part of genes, implicated in growth and development as well as in reproduction of progeny and reactions of organisms to unfavorable factors of environment. The source of methyl groups S-adenosylmethionine (SAM) is in use for biosynthesis of juvenile hormone (JH), methylation of histone proteins and DNA. The main aim of our investigation was evaluation of transcriptional activity of housefly Musca domestica ecdysone receptor gene EcR under adding into ration of 20E and SAM in non-lethal concentrations. Experiments were carried out with larvae and adults of housefly from laboratory strains Shgen and Lgen differ in life span of adults. Change of gene EcR transcripts content in common pool of mRNA in the cells of muscles and gonads, as well as DNA methylation level in 5’-terminal site registered by quantitative real time PCR (RT-PCR). The results of our investigations allow us to suggest existence of mechanism for regulating expression of the EcR gene in M. domestica which is sensitive to exogenic20E and heat stress action as well as to presence of SAM in food. Variations in the mRNA quantitative ratios of EcR gene 5’-and 3’-terminal regions depending on tissue type, gender and age support the hypothesis that this gene can encode several isoforms of the protein EcR. The detected changes in the status of DNA methylation in the 5'-terminal region of the gene and fluctuations in the representation of different mRNA sites after SAM processing suggest the involvement of DNA methylation/demethylation processes in the regulation of EcR gene expression in M. domestica.

Download Full-text

Effect of Regulatory Element DNA Methylation on Tissue-Type Plasminogen Activator Gene Expression

PLoS ONE ◽

10.1371/journal.pone.0167588 ◽

2016 ◽

Vol 11 (12) ◽

pp. e0167588 ◽

Cited By ~ 7

Author(s):

Sylvie Dunoyer-Geindre ◽

Anne-Sophie Rivier-Cordey ◽

Carlos Caetano ◽

Richard J. Fish ◽

Egbert K. O. Kruithof

Keyword(s):

Gene Expression ◽

Dna Methylation ◽

Plasminogen Activator ◽

Regulatory Element ◽

Tissue Type ◽

Tissue Type Plasminogen Activator ◽

Type Plasminogen Activator

Download Full-text

Faculty Opinions recommendation of Tissue-specific imprinting of the mouse insulin-like growth factor II receptor gene correlates with differential allele-specific DNA methylation.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.718529393.793498524 ◽

2014 ◽

Author(s):

Christopher Gregg

Keyword(s):

Dna Methylation ◽

Growth Factor ◽

Receptor Gene ◽

Insulin Like Growth Factor ◽

Tissue Specific ◽

Factor Ii ◽

Allele Specific

Download Full-text

Exploring Children’s Values Questionnaire: Measurement, Gender, and Age Issues

Education Sciences ◽

10.3390/educsci11070309 ◽

2021 ◽

Vol 11 (7) ◽

pp. 309

Author(s):

Loyd Richard Fyffe ◽

Ian Hay

Keyword(s):

World View ◽

Theoretical Construct ◽

School Based ◽

Social Settings ◽

Gender And Age ◽

The Status ◽

Good Internal Consistency ◽

Student Values ◽

Fit Index ◽

Relationship Of

Values are conceptualized as the standards individuals use to determine the status of events and actions and are considered to influence individuals’ behaviours, reasoning, and perceptions. Based on a synthesis of six school-based student values enhancement programs, this paper reports on the development of the Children’s Values Questionnaire (CVQ). This Questionnaire was conceptualized as composing of seven dimensions: Self-Concept; Behaviour; Healthy Life; Social; School Climate; Emotional Intelligence; World View and 26 related sub-dimensions. A total of 848 co-educational students (52% male, 48% female) from Years (Grades) 4 to 7, ages 9 to 13+ years, across 11 Australian schools completed the 95-item CVQ Questionnaire. The Cronbach alpha coefficient of the instrument was 0.94, indicating that the questionnaire had good internal consistency. The inter-correlation between its seven dimensions clustered at Pearson r = 0.55. An exploratory factor analysis was supportive of the CVQ’s theoretical construct (Norm Fit Index of the data to the theoretical construct, 0.09). Girls rated themselves higher than boys (p < 0.001) on items related to Playing by the Rules, Responsibility, Creativity, Empathy, and Communication, and boys rated themselves higher than girls on Physical Activities items (p < 0.001). Older students (Years 6 and 7) compared to younger students (Years 4 and 5) demonstrated greater discernment and differentiation of context (p < 0.05), the growing influence of peer friendship in their value beliefs and an increase in confidence in social settings (p < 0.001). The relationship of the CVQ to Schwartz’s Universal Valued Goals is reported in the paper, along with examples of the application of the CVQ in schools.

Download Full-text

Estrogen Activates the High-Density Lipoprotein Receptor Gene via Binding to Estrogen Response Elements and Interaction with Sterol Regulatory Element Binding Protein-1A

Endocrinology ◽

10.1210/endo.143.6.8855 ◽

2002 ◽

Vol 143 (6) ◽

pp. 2155-2168 ◽

Cited By ~ 47

Author(s):

Dayami Lopez ◽

Mark D. Sanchez ◽

Wendy Shea-Eaton ◽

Mark P. McLean

Keyword(s):

Regulatory Element ◽

Receptor Gene ◽

Density Lipoprotein ◽

Lipoprotein Receptor ◽

Response Elements ◽

Estrogen Response ◽

Density Lipoprotein Receptor ◽

Element Binding Protein ◽

Sterol Regulatory Element ◽

Estrogen Response Elements

Download Full-text

Modulation in the mRNA expression of ecdysone receptor gene in aquatic midge, Chironomus riparius upon exposure to nonylphenol and silver nanoparticles

Environmental Toxicology and Pharmacology ◽

10.1016/j.etap.2011.09.006 ◽

2012 ◽

Vol 33 (1) ◽

pp. 98-106 ◽

Cited By ~ 31

Author(s):

Prakash M. Gopalakrishnan Nair ◽

Jinhee Choi

Keyword(s):

Silver Nanoparticles ◽

Mrna Expression ◽

Receptor Gene ◽

Chironomus Riparius ◽

Ecdysone Receptor

Download Full-text

Retrovirus-induced interference with collagen I gene expression in Mov13 fibroblasts is maintained in the absence of DNA methylation

Molecular and Cellular Biology ◽

10.1128/mcb.11.1.47-54.1991 ◽

1991 ◽

Vol 11 (1) ◽

pp. 47-54

Author(s):

H Chan ◽

S Hartung ◽

M Breindl

Keyword(s):

Gene Expression ◽

Dna Methylation ◽

Chromatin Structure ◽

Transcriptional Activity ◽

Xenopus Laevis Oocytes ◽

Regulatory Sequences ◽

Type I ◽

Wild Type ◽

Col1a1 Gene

We have studied the role of DNA methylation in repression of the murine alpha 1 type I collagen (COL1A1) gene in Mov13 fibroblasts. In Mov13 mice, a retroviral provirus has inserted into the first intron of the COL1A1 gene and blocks its expression at the level of transcriptional initiation. We found that regulatory sequences in the COL1A1 promoter region that are involved in the tissue-specific regulation of the gene are unmethylated in collagen-expressing wild-type fibroblasts and methylated in Mov13 fibroblasts, confirming and extending earlier observations. To directly assess the role of DNA methylation in the repression of COL1A1 gene transcription, we treated Mov13 fibroblasts with the demethylating agent 5-azacytidine. This treatment resulted in a demethylation of the COL1A1 regulatory sequences but failed to activate transcription of the COL1A1 gene. Moreover, the 5-azacytidine treatment induced a transcription-competent chromatin structure in the retroviral sequences but not in the COL1A1 promoter. In DNA transfection and microinjection experiments, we found that the provirus interfered with transcriptional activity of the COL1A1 promoter in Mov13 fibroblasts but not in Xenopus laevis oocytes. In contrast, the wild-type COL1A1 promoter was transcriptionally active in Mov13 fibroblasts. These experiments showed that the COL1A1 promoter is potentially transcriptionally active in the presence of proviral sequences and that Mov13 fibroblasts contain the trans-acting factors required for efficient COL1A1 gene expression. Our results indicate that the provirus insertion in Mov13 can inactivate COL1A1 gene expression at several levels. It prevents the developmentally regulated establishment of a transcription-competent methylation pattern and chromatin structure of the COL1A1 domain and, in the absence of DNA methylation, appears to suppress the COL1A1 promoter in a cell-specific manner, presumably by assuming a dominant chromatin structure that may be incompatible with transcriptional activity of flanking cellular sequences.

Download Full-text

Characterization of a Cis-Regulatory Element and Trans-Acting Protein that Regulates Transcription of the Angiotensin II Type 1A Receptor Gene

Recent Advances in Cellular and Molecular Aspects of Angiotensin Receptors - Advances in Experimental Medicine and Biology ◽

10.1007/978-1-4899-1376-0_1 ◽

1996 ◽

pp. 1-10 ◽

Cited By ~ 1

Author(s):

Satoshi Murasawa ◽

Hiroaki Matsubara ◽

Yasukiyo Mori ◽

Kazuhisa Kijima ◽

Katsuya Maruyama ◽

...

Keyword(s):

Angiotensin Ii ◽

Regulatory Element ◽

Receptor Gene

Download Full-text

Transcriptional Activity of Human Endogenous Retroviruses in Response to Prenatal Exposure of Maternal Cigarette Smoking

American Journal of Perinatology ◽

10.1055/s-0038-1675768 ◽

2018 ◽

Vol 36 (10) ◽

pp. 1060-1065 ◽

Cited By ~ 4

Author(s):

Massimiliano Bergallo ◽

Ilaria Galliano ◽

Valentina Daprà ◽

Alice Pirra ◽

Paola Montanari ◽

...

Keyword(s):

Dna Methylation ◽

Cord Blood ◽

Umbilical Cord ◽

Umbilical Cord Blood ◽

Tobacco Smoke ◽

Transcriptional Activity ◽

In Utero ◽

Endogenous Retroviruses ◽

Human Endogenous Retrovirus ◽

Human Umbilical Cord

Objective Transcription of human endogenous retrovirus (HERV) elements is usually suppressed by epigenetic factors such as DNA methylation and heterochromatin silencing by histone modifications. There is an association between maternal smoking during pregnancy and DNA methylation levels in placental tissue and in DNA from cord blood. Study Design We assessed the transcriptional activity of HERV-H, HERV-K, and HERV-W in umbilical cord blood from 47 term babies unexposed to tobacco smoke in utero and 23 term babies exposed to tobacco smoke in utero. Results In our population, the HERV-H, HERV-K, and HERV-W families were always transcriptionally active, and the levels of all HERVs (H, K, W) were significantly higher in unexposed than smoke-exposed babies. Conclusion This study provides preliminary information about the transcriptional activity of HERV-H, HERV-K, and HERV-W families in human umbilical cord blood.

Download Full-text