scholarly journals Isolation of Vibrio vulnificus Biotype I from Disease Outbreaks on Cultured Tiger Grouper Epinephelus fuscoguttatus Forsskal, 1775

2016 ◽  
Vol 51 (Special-issue) ◽  
pp. S39-S45 ◽  
Author(s):  
Jumroensri Thawonsuwan ◽  
Jiraporn Kasornchandra ◽  
Patcharee Soonsan ◽  
Chantana Keawtapee
2018 ◽  
Vol 6 (2) ◽  
pp. 705 ◽  
Author(s):  
Glenn Valentino ◽  
Abdullah Aman Damai ◽  
Herman Yulianto

Tiger grouper is a marine aquaculture commodities that has good prospects to be developed. One of the areas that could potentially be used as a place of tiger grouper fish farming is the Tegal Island, Pesawaran. The purpose of this research was to analyze the level of water quality for fish farming tiger grouper (Epinephelus fuscoguttatus). This research was carried out in March-May 2017 descriptive analytic nature by doing the observations on the quality of waters which include physical and chemical parameters. The data that used in this research sourced from primary data about the suitability waters of the island while the secondary data is form in research location maps. There were 4 sampling stations which determined with global positioning system (GPS). The results showed that the water quality which is owned by the island's waters on station 1, 2, 3 and 4 were in the quite appropriate category for the cultivation of tiger grouper.


Cryobiology ◽  
2020 ◽  
Vol 95 ◽  
pp. 84-89
Author(s):  
Bin Fan ◽  
Sen Yang ◽  
Le Wang ◽  
Xinghan Chen ◽  
Xiaochun Liu ◽  
...  

Biology ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. 37 ◽  
Author(s):  
Bo Wu ◽  
Qi Wang ◽  
Jie Cao ◽  
Jun Mei ◽  
Jing Xie

Transport in water is the most common method for achieving high survival rates when transporting cultured fish in China; yet, transport success relies on proper water quality and conditions. This research was designed to explore the effects of ascorbic acid and β-1,3-glucan on survival, physiological responses, and flesh quality of farmed tiger grouper (Epinephelus fuscoguttatus) during simulated transport. The transport water temperature for live tiger grouper was 15 °C, which had the highest survival rate, the lowest stress response, and metabolic rate, and this will reduce the susceptibility to diseases. It is stated that β-1,3-glucan influences the changes of cortisol content, heat shock protein 70, IL-1β, and IgM transcription levels during simulated transport. Rather than using ascorbic acid alone (the A-group), β-1,3-glucan (3.2 mg/L) in the presence of ascorbic acid (25 mg/L) can effectively reduce the increase of transport-induced serum cortisol content, heat shock protein 70, and IL-1β, but stimulated IgM. 25 mg/L ascorbic acid and 3.2 mg/L β-1,3-glucan had no obvious effect on the nutritional indexes and flavor of live tiger grouper; however, these can effectively reduce the stress response, improve the innate immune activity, and ensure a higher survival rate.


1998 ◽  
Vol 64 (12) ◽  
pp. 4676-4682 ◽  
Author(s):  
Lise Høi ◽  
Inger Dalsgaard ◽  
Angelo DePaola ◽  
Ronald J. Siebeling ◽  
Anders Dalsgaard

ABSTRACT The findings of this study demonstrate that Vibrio vulnificus isolates recovered from diseased eels in Denmark are heterogeneous as shown by O serovars, capsule types, ribotyping, phage typing, and plasmid profiling. The study includes 85 V. vulnificus isolates isolated from the gills, intestinal contents, mucus, spleen, and kidneys of eels during five disease outbreaks on two Danish eel farms from 1995 to 1997, along with a collection of 12V. vulnificus reference strains. The results showed that more than one serovar may be capable of causing disease in eels and that these isolates are genetically heterogenous as shown by ribotyping. Ribotyping also showed that the same isolates may persist in an eel farm and cause recurrent outbreaks. Phage typing did not correlate with ribotyping or serotyping. However, we observed that 26 of 28 isolates, which were not susceptible to any of the phages, showed the same ribotype, O serovar, and capsule type. This suggests that these isolates may possess features that make them resistant to lysis by the phages used in this study. Ninety-three of 97 isolates harbored between one and three high-molecular-weight plasmids which previously had been suggested to be associated with eel virulence. The subdivision of V. vulnificus into two biotypes based on the indole reaction can no longer be supported, since 82 of 97 isolates in this study were indole positive, and a subdivision into serovars appears to be more correct.


Author(s):  
Albet Surya Kembara ◽  
Putri Desi Wulan Sari

Cantang grouper is a grouper species produced from the hybridization of Kertang grouper and Tiger grouper.  The hybridization,besides increasing species diversification, also has the prospect of cultivation that has the opportunity to increase fishery production in the future. The technique of maintaining grouper larvae needs to be known to be able to increase the production value of the grouper larvae and create technological opportunities in the production process. The embryonic development phase of cantang grouper egg consists of multicellular phase, blastula, gastrula, separation of the tail from the egg sac,  the movement of embryo, internal organ formation and the hatching eggs. During the two months maintenance period, the Hatching Rate (HR) larvae were found to be 80%.


2013 ◽  
Vol 11 (2) ◽  
pp. 109
Author(s):  
. Ilmiah ◽  
. Sukenda ◽  
. Widanarni ◽  
Enang Harris

<p>The use of probiotic bacteria is one of the methods in aquaculture to control infectious diseases. This study aims to obtain candidates probiotic bacteria from the coral reef and tiger grouper aquaculture environment (<em>Epinephelus fuscoguttatus</em>). Steps of the study included of: 1) isolation of candidates probiotic bacteria, 2) selection of candidates probiotic bacteria: inhibition test and co-culture methods, 3) enzymatic test, 4) the growth of bacteria test 5) pathogenicity test and 6) the molecular identification of probiotic bacteria. The results showed that of 124 isolates in test zone of inhibition, the four isolates with the widest zone of inhibition of isolate K7, K8, K21, and T4, a total of 51 isolates on co-culture methods, the four isolates were able to suppress the growth of pathogenic <em>Vibrio</em> bacteria, isolates K15, K68, K55, and T36. The results of hydrolyzing the highest protease amylase and lipase enzyme, respectively isolates K15 and T36; isolates T41 and K8; isolates K55 and K68. Candidates probiotic bacteria reached the exponential phase at 16‒18 h. The results of pathogenicity test isolate K7, K8, T36, and T41 provided the highest survival that can be used as candidates probiotics in the tiger grouper.</p> <p>Keywords: selection, probiotics, tiger grouper</p>


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