scholarly journals Exogenous RNA Segments Detected Specifically in the Brain of Kuchijirosho (snout ulcer disease)-infected Fugu Takifugu rubripes: Molecular Diagnosis Tool for Kuchijirosho

2021 ◽  
Vol 56 (1) ◽  
pp. 6-13
Author(s):  
Tsuyoshi Katou ◽  
Mayuka Kitamura ◽  
Tomoki Maeda ◽  
Tomoyuki Odaka ◽  
Fumio Takizawa ◽  
...  
Keyword(s):  
Molecular Diagnosis ◽  
Takifugu Rubripes ◽  
Ulcer Disease ◽  
Exogenous Rna ◽  
Diagnosis Tool ◽  
The Brain

scholarly journals Comparative transcriptomic analysis of the brain in Takifugu rubripes shows its tolerance to acute hypoxia

2021 ◽  
Author(s):  
Mingxiu Bao ◽  
Fengqin Shang ◽  
Fujun Liu ◽  
Ziwen Hu ◽  
Shengnan Wang ◽  
...  
Keyword(s):  
Acute Hypoxia ◽  
Transcriptomic Analysis ◽  
Takifugu Rubripes ◽  
The Brain ◽  
Comparative Transcriptomic Analysis

scholarly journals Primary Whipple's disease of the brain: characterization of the clinical syndrome and molecular diagnosis

QJM ◽  
2006 ◽  
Vol 99 (9) ◽  
pp. 609-623 ◽  
Author(s):  
P.K. Panegyres
Keyword(s):  
Molecular Diagnosis ◽  
Clinical Syndrome ◽  
Whipple’S Disease ◽  
Whipple's Disease ◽  
The Brain

scholarly journals Evaluation of a gp63–PCR Based Assay as a Molecular Diagnosis Tool in Canine Leishmaniasis in Tunisia

PLoS ONE ◽  
2014 ◽  
Vol 9 (8) ◽  
pp. e105419 ◽  
Author(s):  
Souheila Guerbouj ◽  
Fattouma Djilani ◽  
Jihene Bettaieb ◽  
Bronwen Lambson ◽  
Mohamed Fethi Diouani ◽  
...  
Keyword(s):  
Molecular Diagnosis ◽  
Canine Leishmaniasis ◽  
Diagnosis Tool

scholarly journals Comparative Transcriptomic Analysis of the Brain in Takifugu Rubripes Shows Its Tolerance to Acute Hypoxia

2021 ◽  
Author(s):  
Mingxiu Bao ◽  
Fengqin Shang ◽  
Fujun Liu ◽  
Ziwen Hu ◽  
Shengnan Wang ◽  
...  
Keyword(s):  
Acute Hypoxia ◽  
Enrichment Analysis ◽  
Takifugu Rubripes ◽  
Low Oxygen ◽  
Short Term ◽  
Protection Mechanism ◽  
Complete Set ◽  
Self Protection ◽  
The Brain ◽  
Comparative Transcriptomic Analysis

Abstract Hypoxia is reduced levels of oxygen. Especially in water, due to the complex environment, hypoxic situations often occur. Although fish can survive in low-oxygen waters, this survival ability depends on a complete set of coping mechanisms such as oxygen perception and gene-protein interaction regulation. The research on this mechanism is very meaningful. The present study was undertaken to examine the short-term effects of hypoxia on the brain in Takifugu rubripes. We sequenced the transcriptomes of the brain in T. rubripes to studied their response mechanism to acute hypoxia. Total 167 genes with adjusted P values<0.05 were differentially expressed in the brain of T. rubripes exposed to acute hypoxia. However, hif1a, the master transcriptional regulator of the adaptive response to hypoxia, was not significantly regulated, which indicated that the T. rubripes brain might prevent the HIF-1 signaling pathway. Then Gene Ontology and KEGG Enrichment Analysis were carried out. The results indicated that hypoxia could cause metabolic and neurological changes, showing the clues of their adaptation to acute hypoxia. Overall, the sequenced transcriptomes of the brain in T. rubripes showed small changes under acute hypoxia. As the most complex and important organ, the brain of T. rubripes might be able to create a self-protection mechanism to resist or reduce damage caused by acute hypoxia stress.

THE METABOLISM OF EXOGENOUS RIBONUCLEIC ACIDS INJECTED INTO MICE

1965 ◽  
Vol 43 (7) ◽  
pp. 949-958 ◽  
Author(s):  
S. Sved
Keyword(s):  
Protein Synthesis ◽  
Amino Acid ◽  
Orotic Acid ◽  
The Body ◽  
Ribonucleic Acids ◽  
Exogenous Rna ◽  
Free Nucleotides ◽  
Liver And Kidney ◽  
Labeled Rna ◽  
The Brain

The fate of RNA administered intravenously has been investigated in mice and rats, using yeast RNA uniformly labeled with C14. Much of the administered radioactivity was found in the CO2 exhaled within 5 hours, the rest being distributed mostly between the free nucleotides and carbohydrates of the nine tissues tested. Brain showed consistently low values in all chemical fractions. Administration of large amounts of RNA over a period of 5 days caused an increase in the incorporation of radioactivity into the nucleic acids of liver and kidney and into the amino acid pool of the body. A slight increase in protein synthesis in the brain was also noted. From results obtained by studying the rate of appearance of the radioactivity in the CO2 after injections of labeled RNA, ribose, orotic acid, and RNA mixed with unlabeled ribose, it was concluded that most of the exogenous RNA, when injected, is rapidly degraded before re-utilization of some of its components.

scholarly journals Dynamic Changes in the Levels of Amyloid-β42 Species in the Brain and Periphery of APP/PS1 Mice and Their Significance for Alzheimer’s Disease

2021 ◽  
Vol 14 ◽  
Author(s):  
Liding Zhang ◽  
Changwen Yang ◽  
Yanqing Li ◽  
Shiqi Niu ◽  
Xiaohan Liang ◽  
...  
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Sandwich Elisa ◽  
Amyloid Β ◽  
Dynamic Changes ◽  
Microbiome Composition ◽  
The Core ◽  
Diagnosis Tool ◽  
The Brain ◽  
Gut Microbiota Composition

Although amyloid-β42 (Aβ42) has been used as one of the core biomarkers for Alzheimer’s disease (AD) diagnosis, the dynamic changes of its different forms in the brain, blood, and even intestines and its correlation with the progression of AD disease remain obscure. Herein, we screened Aβ42-specific preferred antibody pairs 1F12/1F12 and 1F12/2C6 to accurately detect Aβ42 types using sandwich ELISA, including total Aβ42, Aβ42 oligomers (Aβ42Os), and Aβ42 monomers (Aβ42Ms). The levels of Aβ42 species in the brain, blood, and intestines of different aged APP/PS1 mice were quantified to study their correlation with AD progression. Total Aβ42 levels in the blood were not correlated with AD progression, but Aβ42Ms level in the blood of 9-month-old APP/PS1 mice was significantly reduced, and Aβ42Os level in the brain was significantly elevated compared to 3-month-old APP/PS1, demonstrating that the levels of Aβ42Ms and Aβ42Os in the blood and brain were correlated with AD progression. Interestingly, in 9-month-old APP/PS1 mice, the level of Aβ42 in the intestine was higher than that in 3-month-old APP/PS1 mice, indicating that the increased level of Aβ42 in the gastrointestinal organs may also be related to the progression of AD. Meanwhile, changes in the gut microbiota composition of APP/PS1 mice with age were also observed. Therefore, the increase in Aβ derived from intestinal tissues and changes in microbiome composition can be used as a potential early diagnosis tool for AD, and further used as an indicator of drug intervention to reduce brain amyloid.

scholarly journals Profile of Gene Expression Changes During Estrodiol-17β-Induced Feminization in the Takifugu Rubripes Brain

2021 ◽  
Author(s):  
Xufang Shen ◽  
Hongwei Yan ◽  
Jieming Jiang ◽  
Weiyuan Li ◽  
Yuyu Xiong ◽  
...  
Keyword(s):  
Arachidonic Acid ◽  
Acid Metabolism ◽  
Sex Differentiation ◽  
Arachidonic Acid Metabolism ◽  
Differentially Expressed ◽  
Receptor Interaction ◽  
Takifugu Rubripes ◽  
Molecular Control ◽  
And Control ◽  
The Brain

Abstract Background: As the critical tissue of the central nervous system, the brain has been found to be involved in gonad development. Previous studies have suggested that gonadal fate may be affected by the brain. Identifying brain-specific molecular changes that occur during estrodiol-17β (E2) ‑induced feminization is crucial to our understanding of the molecular control of sex differentiation by the brains of fish. Results: In this study, the differential transcriptomic responses of the Takifugu rubripes larvae brain were compared after E2 treatment for 55 days. Our results showed that 514 genes were differentially expressed between E2-treated-XX (E-XX) and Control-XX (C-XX) T. rubripes, while 362 genes were differentially expressed between E2-treated-XY (E-XY) and Control-XY (C-XY). For example, the expression of cyp19a1b, gnrh1 and pgr was significantly up-regulated, while st, sl, tshβ, prl and pit-1, which belong to the growth hormone/prolactin family, were significantly down-regulated after E2 treatment, in both sexes. The arntl1, bhlbe, nr1d2, per1b, per3, cry1, cipc and ciart genes, which are involved in the circadian rhythm, were also found to be altered. Differentially expressed genes (DEGs), which were identified between E-XX and C-XX, were significantly enriched in neuroactive ligand-receptor interaction, arachidonic acid metabolism, cytokine-cytokine receptor interaction and the calcium signaling pathway. The DEGs that were identified between E‑XY and C-XY were significantly enriched in tyrosine metabolism, phenylalanine metabolism, arachidonic acid metabolism and linoleic acid metabolism. Conclusion: By using transcriptome sequencing of XX and XY brains of torafugu larvae showed that many genes and pathways were altered by E2 exposure. They provide the opportunity to further study the possible involvement of networks in the brain-pituitary-gonadal axis in sex differentiation in T. rubripes.

scholarly journals Isolation of Causative Virus from Cultured Tiger Puffer(Takifugu rubripes)Affected by Kuchijirosho(Snout Ulcer Disease).

1992 ◽  
Vol 27 (2) ◽  
pp. 97-102 ◽  
Author(s):  
Kiyoshi Inouye ◽  
Kazuma Yoshikoshi ◽  
Ikuo Takami
Keyword(s):  
Takifugu Rubripes ◽  
Ulcer Disease ◽  
Tiger Puffer
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