scholarly journals Phospholipid biomarkers analysis as a tool for microbial community assessment on radionuclides contaminated territories

2020 ◽  
Vol 11 (3) ◽  
Author(s):  
Y. Ruban ◽  
◽  
K. Shavanova ◽  
V. Illienko ◽  
K. Korepanova ◽  
...  
Keyword(s):  
Plant Communities ◽  
Phospholipid Fatty Acid ◽  
Soil Microflora ◽  
Soil Samples ◽  
Gram Negative Bacteria ◽  
Cooling Pond ◽  
Radionuclide Contamination ◽  
Total Plfa ◽  
Taxonomic Groups ◽  
Plfa Analysis

The presence of specific components in the environment can change the state of cenoses. The effect of ionizing radiation on plant communities, animals and humans have been well studied, while the effect on soil microflora has not been insufficiently studied. In this paper investigated the effect of radionuclide contamination on microflora of soil samples from the territory of PTLRW "Red Forest 1" (trench), "Red Forest 2" (outside the trench), " Cooling Pond " and " Zalissia ". Phospholipid fatty acid (PLFA) content was obtained by PLFA analysis. The highest level of total PLFA was observed in the territory of "Zalissia" which was 17.40 ± 10.59 μg / h. From the territory of PTLRW "Red Forest 1" (trench) and "Red Forest 2" (outside the trench) the level of total PLFA was 16.29 ± 3.43 μg / g and 16.40 ± 2.90 μg / h, respectively. The PLFA content of the "Cooling Pond" was significantly different from the "Red Forest" and " Zalissia ". The taxonomic groups assessment of the samples, a fungus, gram-positive and gram-negative bacteria content increased in PTLRW "Red Forest" relative to the point "Zalissia ".

scholarly journals Phospholipid fatty acid (PLFA) analysis as a tool to estimate absolute abundances from compositional 16S rRNA bacterial metabarcoding data

2021 ◽  
pp. 106271
Author(s):  
Natascha Lewe ◽  
Syrie Hermans ◽  
Gavin Lear ◽  
Laura T. Kelly ◽  
Georgia Thomson-Laing ◽  
...  
Keyword(s):  
Fatty Acid ◽  
16S Rrna ◽  
Phospholipid Fatty Acid ◽  
Plfa Analysis

scholarly journals Microbial Population Changes during Bioremediation of an Experimental Oil Spill

1999 ◽  
Vol 65 (8) ◽  
pp. 3566-3574 ◽  
Author(s):  
Sarah J. MacNaughton ◽  
John R. Stephen ◽  
Albert D. Venosa ◽  
Gregory A. Davis ◽  
Yun-Juan Chang ◽  
...  
Keyword(s):  
Microbial Community ◽  
Bacterial Community ◽  
Oil Spill ◽  
Phospholipid Fatty Acid ◽  
Community Structures ◽  
Gram Negative ◽  
Dgge Analysis ◽  
16S Rdna Pcr ◽  
Microbial Community Structures ◽  
Plfa Analysis

ABSTRACT Three crude oil bioremediation techniques were applied in a randomized block field experiment simulating a coastal oil spill. Four treatments (no oil control, oil alone, oil plus nutrients, and oil plus nutrients plus an indigenous inoculum) were applied. In situ microbial community structures were monitored by phospholipid fatty acid (PLFA) analysis and 16S rDNA PCR-denaturing gradient gel electrophoresis (DGGE) to (i) identify the bacterial community members responsible for the decontamination of the site and (ii) define an end point for the removal of the hydrocarbon substrate. The results of PLFA analysis demonstrated a community shift in all plots from primarily eukaryotic biomass to gram-negative bacterial biomass with time. PLFA profiles from the oiled plots suggested increased gram-negative biomass and adaptation to metabolic stress compared to unoiled controls. DGGE analysis of untreated control plots revealed a simple, dynamic dominant population structure throughout the experiment. This banding pattern disappeared in all oiled plots, indicating that the structure and diversity of the dominant bacterial community changed substantially. No consistent differences were detected between nutrient-amended and indigenous inoculum-treated plots, but both differed from the oil-only plots. Prominent bands were excised for sequence analysis and indicated that oil treatment encouraged the growth of gram-negative microorganisms within the α-proteobacteria andFlexibacter-Cytophaga-Bacteroides phylum. α-Proteobacteria were never detected in unoiled controls. PLFA analysis indicated that by week 14 the microbial community structures of the oiled plots were becoming similar to those of the unoiled controls from the same time point, but DGGE analysis suggested that major differences in the bacterial communities remained.

scholarly journals Linking Toluene Degradation with Specific Microbial Populations in Soil

1999 ◽  
Vol 65 (12) ◽  
pp. 5403-5408 ◽  
Author(s):  
Jessica R. Hanson ◽  
Jennifer L. Macalady ◽  
David Harris ◽  
Kate M. Scow
Keyword(s):  
Microbial Community ◽  
Microbial Population ◽  
Phospholipid Fatty Acid ◽  
Microbial Populations ◽  
Complex Environments ◽  
Toluene Degradation ◽  
Effective Technique ◽  
Soil Microbial ◽  
Isotope Tracer ◽  
Plfa Analysis

ABSTRACT Phospholipid fatty acid (PLFA) analysis of a soil microbial community was coupled with 13C isotope tracer analysis to measure the community’s response to addition of 35 μg of [13C]toluene ml of soil solution−1. After 119 h of incubation with toluene, 96% of the incorporated13C was detected in only 16 of the total 59 PLFAs (27%) extracted from the soil. Of the total 13C-enriched PLFAs, 85% were identical to the PLFAs contained in a toluene-metabolizing bacterium isolated from the same soil. In contrast, the majority of the soil PLFAs (91%) became labeled when the same soil was incubated with [13C]glucose. Our study showed that coupling13C tracer analysis with PLFA analysis is an effective technique for distinguishing a specific microbial population involved in metabolism of a labeled substrate in complex environments such as soil.

scholarly journals Assessing the Microbial Communities in Four Different Daqusby Using PCR-DGGE, PLFA, and Biolog Analyses

2020 ◽  
Vol 69 (1) ◽  
pp. 27-37
Author(s):  
YUXI LING ◽  
WENYING LI ◽  
TONG TONG ◽  
ZUMING LI ◽  
QIAN LI ◽  
...  
Keyword(s):  
Microbial Communities ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Phospholipid Fatty Acid ◽  
Bacterial Species ◽  
Carbon Sources ◽  
Bacterial Biomass ◽  
Biolog Ecoplates ◽  
Different Types ◽  
Gradient Gel Electrophoresis ◽  
Plfa Analysis

Daqu made from raw wheat, barley or pea is used as an inoculum for the fermentation of Chinese Baijiu. In this study, the microbial communities of four different types of Daqus (sauce-flavor Wuling Daqu, sauce and strong-flavor Baisha Daqu, strong-flavor Deshan Daqu, and light-flavor Niulanshan Daqu) were analyzed by polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE), phospholipid fatty acid (PLFA) analysis, and Biolog EcoPlates analysis (Biolog). Clear differences were seen between the microbial communities of the four Daqus. PCR-DGGE showed differences in the number and brightness of bands between the Daqus, indicating the presence of unique bacterial species in Deshan Daqu, Wuling Daqu, and Niulanshan Daqu. Lactobacillus sanfranciscensis, Bacillus thermoamylovorans, and some unclassified bacteria were unique to Wuling Daqu, Deshan Daqu, and Niulanshan Daqu, respectively. Moreover, some bacterial species were observed in all four Daqus. A total of 26 PLFAs between C12 to C20 were detected from the four Daqus by PLFA analysis. Wuling Daqu had the highest total and fungal biomasses, Baisha Daqu had the highest bacterial biomass, and Niulanshan Daqu had the highest ratio of fungal biomass to bacterial biomass. The Biolog results indicated differences in the carbon source use and mode of the four Daqus, and also demonstrated that each Daqu had varying abilities to utilize different types of carbon sources. The cluster analysis of the three methods showed that the microbial communities of the four Daqus were different. This study also demonstrates the applicability of the three analytical methods in the evaluating of the microbial communities of Daqus.

scholarly journals Phospholipid Fatty Acid (PLFA) Analysis of Rhizosphere Bacterial Communities in a Peat Soil

2002 ◽  
Vol 51 (1-2) ◽  
pp. 123-128 ◽  
Author(s):  
András Halbritter ◽  
T. Mogyoróssy
Keyword(s):  
Fatty Acid ◽  
Bacterial Communities ◽  
Phospholipid Fatty Acid ◽  
Plant Root ◽  
Peat Soil ◽  
Total Lipid Content ◽  
Gas Chromatography Mass Spectrometry ◽  
Root Cells ◽  
Plfa Analysis ◽  
Rhizosphere Bacterial Communities

To analyze the rhizosphere bacterial communities in wetlands, the total lipid content was extracted from a peat soil and 4 abundant wetland plant roots ( Typha angustifolia L., Salix cinerea L., Carex pseudocyperus L., Thelypteris palustris Salisb.). The separated phospholipid fraction was further fractionated and deriva­tized prior to gas chromatography-mass spectrometry (GC-MS) measurement. In the evaluation only the bacteria-specific fatty acids were used in order to neglect fatty acid information derived from plant root cells. Based on these analyses, a high level bacterial concentration was demonstrated in the rhizosphere, and the relative occurrence of aerobe and anaerobe, Gram positive and negative bacteria, methanotrophs, sulphate reducers and Actinobacteria was determined. Through the PLFA analysis the study of bacteria regardless of culturability was possible.

The Use of Fluorogenic Substrates To Measure Fungal Presence and Activity in Soil

1998 ◽  
Vol 64 (2) ◽  
pp. 613-617 ◽  
Author(s):  
Morten Miller ◽  
Ansa Palojärvi ◽  
Andrea Rangger ◽  
Morten Reeslev ◽  
Annelise Kjøller
Keyword(s):  
Fungal Biomass ◽  
Phospholipid Fatty Acid ◽  
Soil Samples ◽  
Fluorogenic Substrates ◽  
Link Type ◽  
Solid Media ◽  
Enzyme Substrates ◽  
Agar Media ◽  
Number Of Bacteria ◽  
Selection Of

ABSTRACT Our objective was to determine if 4-methylumbelliferyl-labelled enzyme substrates could be used to detect and quantify specific components of chitinase and cellulase activities as specific indicators of the presence and activity of fungal biomass. The fluorogenic substrates 4-methylumbelliferyl (MUF)N-acetyl-β-d-glucosaminide and MUF β-d-lactoside were used for the detection and quantification of β-N-acetylglucosaminidase (EC 3.2.1.30 ) (NAGase) and endo 1,4-β-glucanase (EC 3.2.1.4 )/cellobiohydrolase (EC3.2.1.91 ) (CELase), respectively. Culture screenings on solid media showed a widespread ability to produce NAGase among a taxonomically diverse selection of fungi on media with and without added chitin. NAGase activity was expressed only in a limited number of bacteria and on media supplemented with chitin. The CELase activity was observed only in a limited number of fungi and bacteria. Bacterial CELase activity was expressed on agar media containing a cellulose-derived substrate. In soil samples, NAGase activity was significantly correlated with estimates of fungal biomass, based on the content of two fungus-specific indicator molecules, 18:2ω6 phospholipid fatty acid (PLFA) and ergosterol. CELase activity was significantly correlated with the PLFA-based estimate of fungal biomass in the soil, but no correlation was found with ergosterol-based estimates of fungal biomass.

Determination of phospholipid ester-linked fatty acids and poly β-hydroxybutyrate for the estimation of bacterial biomass and activity in the rhizosphere of the rape plant Brassica napus (L.)

1985 ◽  
Vol 31 (12) ◽  
pp. 1113-1119 ◽  
Author(s):  
Anders Tunlid ◽  
Bruce H. Baird ◽  
Melanie B. Trexler ◽  
Stefan Olsson ◽  
Robert H. Findlay ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Growth Response ◽  
Phospholipid Fatty Acid ◽  
Bacterial Biomass ◽  
Gram Negative Bacteria ◽  
Brassica Napus L ◽  
Rape Plant ◽  
Acid Ratio ◽  
Definition Of

Examination of seven strains of gram-negative bacteria isolated from the rhizosphere of the rape plant showed profiles of fatty acids, ester-linked to their phospholipids that were distinctly different from sterile roots. The bacteria were enriched in short and branched saturated, cyclopropane and monoenoic fatty acids in which the unsaturation was formed by the anerobic desaturase pathway when growth was on a medium simulating root exudates. This suite of fatty acids was significantly increased in sand and in roots of rape plants grown from seeds in sands inoculated with these organisms at the start of the experiments. Some of the bacteria formed the endogenous storage polymer poly β-hydroxybutyrate in monocultures. Poly β-hydroxybutyrate was formed by the bacteria in the inoculated sand in large amounts but not by the bacteria that were recovered from the roots. This suggests that bacteria associated with the roots were in balanced growth, whereas the bacteria in the sand were lacking essential nutrients and showed an unbalanced growth response. These chemical analyses provided estimates of the bacterial biomass (from the sum of bacterial ester-linked phospholipid fatty acids) and the metabolic status (poly β-hydroxybutyrate to phospholipid fatty acid ratio) in this experiment. With further definition of lipid biomarkers of soil microorganisms these experimental techniques may be extended to the rhizosphere.

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