scholarly journals ULTRASTRUCTURE OF THE ABDOMINAL PLEXUS OF RATS DURING HERBICIDE INTOXICATION AND TREATMENT

Author(s):  
S.M. Shakirova ◽  
◽  
D.M. Shakirova ◽  
◽  

As a result of the study, it was found that poisoning of rats with the herbicide 2,4 - DA for 4 weeks causes a decrease in the number of neurocytes in the celiac plexus, changes the protein synthesizing and energy systems of the cell, and accumulates residual structures in the form of lipofuscin. In the neuroplasm, the number of neurofilaments and neurotubules decreases, the organization of the myelin layer of fibers is disrupted. Changes affect the structure of the intima and media of blood vessels, leading to a violation of their transport function. When rats are treated with Taktivin, they show positive dynamics in the structure of the celiac plexus; the protein-synthesizing system is restored in the nuclei and neuroplasm of a number of cells. In the connective tissue stroma, the ultrastructure of collagen fibers improves, and the number of blood vessels increases.

Author(s):  
U.P. Mainde ◽  
S.B. Banubakode ◽  
N.C. Nandeshwar ◽  
R.Y. Charjan ◽  
S. Sathapathy ◽  
...  

Background: Skin is a versatile organ, which is extremely important for protection, perception, water regulation and wound healing. The skin of goat is considered extremely durable used by the tannery Industry to make rugs and carpet binding.Methods: The present study was conducted on the skin of goats of either sex to study the age wise changes in the histomorphology, histochemistry and histoenzymology of dermis. The skin samples were collected from Nagpur Muncipal Corporation Slaughter house immediately after natural death and subsequently processed and stained for histological, histochemical and histoenzymic studies. The total of 220 samples, 20 each from different body regions namely dorsal neck, lateral neck, ventral neck, dorsal thorax, lateral thorax, ventral thorax, dorsal abdomen, lateral abdomen, ventral abdomen, lateral thigh and medial thigh were collected. The animals were divided into two groups, viz. six to twelve month of age (Gr I) and above one year age (Gr II).Result: It was observed that the dermis was composed of collagen, elastic, reticular fibers, cells of connective tissue, blood vessels, nerves, hair follicles, sweat glands, sebaceous glands and arrector pili muscles in all body regions in Gr I as well as in Gr II. The dermis was divided into thin superficial papillary layer and a thick deep reticular layer. The dermal papillae were the upward projections of superficial papillary layer between epidermal pegs. In reticular layer, fibroblasts were larger, while in papillary layer, fibroblasts were thin long, compressed and spindle shaped. The collagen fibers were more in number as compared to other fibers in dermis and they were densely arranged in papillary layer and parallel with the skin surface below the epidermis. The elastic fibers were present in between the collagen fibers around the blood vessels and hair follicles. The arteriovenous shunt was found surrounded by capsule of connective tissue consisting of smooth muscle cells. The more number of hair follicles at deep part of papillary layer and few follicles were present in reticular layer. The hair follicle composed of outer root sheath, inner root sheath, cortex and medulla. The depth of primary hair follicle increased with the advancement of age. Moderate PAS positive activity was noted in the papillary layer, while it was weak to moderate in reticular layer. The reticular layer of the dermis showed moderate Sudan black B activity for the presence of lipid than the papillary layer. The mild acid phosphatase activity was seen in the papillary and reticular layer of dermis, while moderate acid phosphatase activity was noticed in the blood vessels. A mild alkaline phosphatase activity was present in dermis in Gr I and Gr II. Conclusion: The present study would form a baseline data on the histomorphology, histochemistry and histoenzymology of skin of goat which would be helpful in future research prospective.


1973 ◽  
Vol 10 (3) ◽  
pp. 232-237 ◽  
Author(s):  
F. A. Zaki ◽  
S.-K. Liu

Chromophobe adenoma was diagnosed in the pituitary gland of an 11-year-old domestic shorthaired cat. Histologically, the tumor consisted of a mass of cells with scant sinusoidal arrangement and connective tissue stroma and few blood vessels. In areas the neoplastic cells contained large vacuoles that occupied the entire cytoplasm and pushed the nuclei aside.


2017 ◽  
Vol 19 (77) ◽  
pp. 117-120
Author(s):  
Zh.H. Stegney

Tissue components, intraorganic blood vessels and indicators of correlation between the area of these structures in the thymus of 1, 5, 10 and 20 day-old calves were investigated. Newborns calves at birth had different morphofunctional status. Thymic blood vessels are represented as interlobular and intralobular blood vessels as confirmed by other authors. In the cortical portion blood vessels were branching radially, in the medullary portion they formed a polygonal plexus. In 1-day-old calves of average morphofunctional status the area of organ’s parenchyma was less and the area of connective tissue stroma was bigger than in the calves of high status. This indicates the delay of thymus formation as an organ and suppression of its functional activity in calves of average morphofunctional status. In this case, the area of thymic blood vessels of 1-day-old calves of high morphofunctional status was bigger than of average morphofunctional status calves. The area of thymic parenchyma in calves up to 5-days old was increasing, and the area of connective tissue stroma was reducing. Characteristically, the area of parenchyma and stroma in the calves of this age reached the values that calves of high morphofunctional status had. In older calves the area of parenchyma was slightly decreasing and the area of stroma was increasing. Our studies showed that the correlative relationships between the area of blood vessels and tissue components of thoracic part of thymus of calves with different morphofunctional status were variable in the firmness and character. In thoracic part of thymus of 1-day-old calves with high morphofunctional status of the body the correlative relationships between the area of blood vessels and tissue components were tight and reversible. Their firmness was slightly less in calves of medium status. With increasing of calves’ age the correlative relationships changed asynchronously in firmness and character: in 5- and 10-days-old calves they were tight, weak and reversible, and in 20-days-old calves they were tight, positive and reversible.


Author(s):  
E. J. Kollar

The differentiation and maintenance of many specialized epithelial structures are dependent on the underlying connective tissue stroma and on an intact basal lamina. These requirements are especially stringent in the development and maintenance of the skin and oral mucosa. The keratinization patterns of thin or thick cornified layers as well as the appearance of specialized functional derivatives such as hair and teeth can be correlated with the specific source of stroma which supports these differentiated expressions.


Symmetry ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 1126
Author(s):  
Giovanna Iezzi ◽  
Francesca Di Lillo ◽  
Michele Furlani ◽  
Marco Degidi ◽  
Adriano Piattelli ◽  
...  

Symmetric and well-organized connective tissues around the longitudinal implant axis were hypothesized to decrease early bone resorption by reducing inflammatory cell infiltration. Previous studies that referred to the connective tissue around implant and abutments were based on two-dimensional investigations; however, only advanced three-dimensional characterizations could evidence the organization of connective tissue microarchitecture in the attempt of finding new strategies to reduce inflammatory cell infiltration. We retrieved three implants with a cone morse implant–abutment connection from patients; they were investigated by high-resolution X-ray phase-contrast microtomography, cross-linking the obtained information with histologic results. We observed transverse and longitudinal orientated collagen bundles intertwining with each other. In the longitudinal planes, it was observed that the closer the fiber bundles were to the implant, the more symmetric and regular their course was. The transverse bundles of collagen fibers were observed as semicircular, intersecting in the lamina propria of the mucosa and ending in the oral epithelium. No collagen fibers were found radial to the implant surface. This intertwining three-dimensional pattern seems to favor the stabilization of the soft tissues around the implants, preventing inflammatory cell apical migration and, consequently, preventing bone resorption and implant failure. This fact, according to the authors’ best knowledge, has never been reported in the literature and might be due to the physical forces acting on fibroblasts and on the collagen produced by the fibroblasts themselves, in areas close to the implant and to the symmetric geometry of the implant itself.


2014 ◽  
Vol 2014 ◽  
pp. 1-4
Author(s):  
Ramalingam Suganya ◽  
Narasimhan Malathi ◽  
Subramani Vijaya Nirmala ◽  
Chinnaswami Ravindran ◽  
Harikrishnan Thamizhchelvan

Choristomas are rare entities which are aggregates of microscopically normal cells or tissues in aberrant locations. They are a “heterotopic” rest of cells, as they appear as a tumor-like mass. Herein we report a case of cartilaginous choristoma in a 54-year-old male who presented with a swelling on right lower gingiva. The histopathological examination revealed features of a well circumscribed mass of mature cartilage in a dense fibrous connective tissue stroma.


In Vivo ◽  
2021 ◽  
Vol 35 (1) ◽  
pp. 313-317
Author(s):  
SATOSHI YOKOSE ◽  
YUKA KATO ◽  
KATSUTOSHI MATSUMOTO ◽  
PERRY R. KLOKKEVOLD ◽  
HENRY H. TAKEI ◽  
...  

2020 ◽  
Vol 9 (3) ◽  
pp. 64-71
Author(s):  
N. N. Shevlyuk ◽  
L. V. Khalikova ◽  
A. A. Khalikov

The aim of the study was to establish morphofunctional and immunohistochemical characteristics of large omentum in women with ovarian cancer.Material and methods. The large omenta of 48 women with ovarian cancer (low-grade differentiated seropapillary adenocarcinoma of high-grade malignancy) of II stage (n=20) and III stage (n=28) were studied. Histological sections were stained with overview histological and immunohistochemical methods (to reveal ki67, P53, CD34, CD7, CD4, CD8, CD61 proteins expression). Results. In patients, the size of the large omentum was characterized by high individual variability; in the presence of metastasis, the size of the omentum was reduced. Intensive development of blood vessels in the organ was noted, but in the presence of metastases stasis of blood corpuscles, leucocytic infiltration, and moderate edema of connective tissue were observed in the organ’s vessels. Areas of lymphoid tissue, both small lymphatic follicles and diffusely located lymphoid tissue, were revealed in the omentum. In most follicles, reactive centers were not marked, and the number of follicles was reduced in the presence of metastases in the omentum. The analysis of CD34+ cells distribution showed that they were identified both in the tumor and in the areas of the omentum adjacent to the tumor, which indicates a pronounced angiogenesis. An irregular distribution of CD7+ and CD8+ and CD4+ cells was revealed in the tumor tissues, as well as in the surroundings. Simultaneously with the expression of P53 protein, ki67 protein expression is revealed in the significant number of tumor cells (including endothelial cells of tumor blood vessels). The proportion of ki67+ cells in the tumor cell population was 60.1±3.3%. The presence of a large number of ki67+cells in the presence of P53 protein expression in them indicates the aggressiveness of the tumor, as well as a disturbance of apoptosis regulatory mechanisms in the cells. Ki67 expression was low in the omentum areas unaffected by metastases, and it was revealed in the certain areas of connective tissue in fibroblastic programmed differentiation cells. Conclusion. The results obtained indicate significant plasticity and reactivity of great omentum in the presence of tumor process in the body and confirm the important role of great omentum in protective reactions.


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