Near-infrared calibration models for estimating volatile fatty acids and methane production from in vitro rumen fermentation of different total mixed rations

Nutritional strategies can be employed to mitigate greenhouse emissions from ruminants. This article investigates the effects of polyphenols extracted from the involucres of Castanea mollissima Blume (PICB) on in vitro rumen fermentation. Three healthy Angus bulls (350 ± 50 kg), with permanent rumen fistula, were used as the donors of rumen fluids. A basic diet was supplemented with five doses of PICB (0%–0.5% dry matter (DM)), replicated thrice for each dose. Volatile fatty acids (VFAs), ammonia nitrogen concentration (NH3-N), and methane (CH4) yield were measured after 24 h of in vitro fermentation, and gas production was monitored for 96 h. The trial was carried out over three runs. The results showed that the addition of PICB significantly reduced NH3-N (p < 0.05) compared to control. The 0.1%–0.4% PICB significantly decreased acetic acid content (p < 0.05). Addition of 0.2% and 0.3% PICB significantly increased the propionic acid content (p < 0.05) and reduced the acetic acid/propionic acid ratio, CH4 content, and yield (p < 0.05). A highly significant quadratic response was shown, with increasing PICB levels for all the parameters abovementioned (p < 0.01). The increases in PICB concentration resulted in a highly significant linear and quadratic response by 96-h dynamic fermentation parameters (p < 0.01). Our results indicate that 0.2% PICB had the best effect on in-vitro rumen fermentation efficiency and reduced greenhouse gas production.

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Inoculum for In Vitro Rumen Fermentation and Composition of Volatile Fatty Acids

Journal of Dairy Science ◽

10.3168/jds.s0022-0302(80)82931-6 ◽

1980 ◽

Vol 63 (2) ◽

pp. 305-312 ◽

Cited By ~ 14

Author(s):

T. Senshu ◽

K. Nakamura ◽

A. Sawa ◽

H. Miura ◽

T. Matsumoto

Keyword(s):

Fatty Acids ◽

Volatile Fatty Acids ◽

Rumen Fermentation ◽

In Vitro Rumen Fermentation

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Evaluation of ensiled soy sauce by-product combined with several additives as an animal feed

Veterinary World ◽

10.14202/vetworld.2020.940-946 ◽

2020 ◽

Vol 13 (5) ◽

pp. 940-946

Author(s):

Sadarman Sadarman ◽

Muhammad Ridla ◽

Nahrowi Nahrowi ◽

Roni Ridwan ◽

Anuraga Jayanegara

Keyword(s):

Chemical Composition ◽

Propionic Acid ◽

Methane Production ◽

Volatile Fatty Acids ◽

Animal Feed ◽

Rumen Fermentation ◽

Soy Sauce ◽

Ruminal Fermentation ◽

In Vitro Rumen Fermentation

Aim: The present experiment aimed to evaluate the use of different additives, i.e., lactic acid bacteria (LAB) inoculant, tannin extract, and propionic acid, on the chemical composition, fermentative characteristics, and in vitro ruminal fermentation of soy sauce by-product (SSB) silage. Materials and Methods: SSB was subjected to seven silage additive treatments: Fresh SSB, ensiled SSB, ensiled SSB+LAB, ensiled SSB+2% acacia tannin, ensiled SSB+2% chestnut tannin, ensiled SSB+0.5% propionic acid, and ensiled SSB+1% acacia tannin+1% chestnut tannin+0.5% propionic acid. Ensiling was performed for 30 days in three replicates, and each replicate was made in duplicate. The samples were evaluated for their chemical composition and silage fermentation characteristics and were tested in an in vitro rumen fermentation system. Results: In general, the nutrient compositions did not differ among the tested SSBs in response to the different additives used. The addition of tannins, either acacia or chestnut, and propionic acid significantly decreased the pH of the ensiled SSB (p<0.05). The addition of several additives (except LAB) decreased the ammonia concentration in SSB silage (p<0.05). The total volatile fatty acids in the in vitro rumen fermentation profile of the ensiled SSB were not significantly altered by the various additives applied. The addition of some additives, i.e., ensiled SSB+LAB and ensiled SSB+2% acacia tannin, reduced the digestibility values of the SSB (p<0.05). Different silage additives did not significantly affect methane production, although the addition of acacia tannins tended to result in the lowest methane production among treatments. Conclusion: The use of additives, particularly 2% acacia tannins, can reduce proteolysis in SSB silage.

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In vitro methane and gas production characteristics of Eragrostis trichopophora substrate supplemented with different browse foliage

Animal Production Science ◽

10.1071/an15612 ◽

2016 ◽

Vol 56 (3) ◽

pp. 634 ◽

Cited By ~ 5

Author(s):

Abubeker Hassen ◽

Jacobus Johannes Francois Theart ◽

Willem Adriaan van Niekerk ◽

Festus Adeyemi Adejoro ◽

Belete Shenkute Gemeda

Keyword(s):

Fatty Acids ◽

Methane Production ◽

Volatile Fatty Acids ◽

Rumen Fluid ◽

Condensed Tannin ◽

Rumen Fermentation ◽

Gas Production ◽

Optimum Level ◽

Browse Species

An in vitro gas production study was conducted to evaluate the potential of six browse species (high, medium and low condensed tannin concentrations) collected from the Kalahari Desert as antimethanogenic additives to an Eragrostis trichopophora-based substrate. The browse species studied were Acacia luederitzii, Monechma incanum, Acacia erioloba, Acacia haematoxylon, Olea europaea and Acacia mellifera. The edible forage dry matter of the browse species were incubated with Eragrostis trichopophora in a 30 : 70 (w/w) ratio by adding 40 mL of a buffered rumen fluid at 39°C for 48 h. Gas and methane production at different time intervals after incubation were determined whereas the volatile fatty acids concentration was evaluated after 48 h. Acacia luederitzii and M. incanum foliage decreased methane production by more than 50%, but simultaneously decreased digestibility, and rumen fermentation parameters such as volatile fatty acids concentration. Tannin extracts from A. luederitzii could possibly be used as a dietary alternative to reduce methane production; however, there is a need to determine an optimum level of inclusion that may not compromise the efficiency of rumen fermentation and overall digestibility of the diet.

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Reducing enteric methane production from buffalo (Bubalus bubalis) by garlic oil supplementation in in vitro rumen fermentation system

SN Applied Sciences ◽

10.1007/s42452-021-04264-6 ◽

2021 ◽

Vol 3 (2) ◽

Author(s):

Avijit Dey ◽

Shyam Sundar Paul ◽

Puran Chand Lailer ◽

Satbir Singh Dahiya

Keyword(s):

Environmental Pollution ◽

Methane Production ◽

Rumen Fluid ◽

Bubalus Bubalis ◽

Rumen Fermentation ◽

Acetyl Esterase ◽

Garlic Oil ◽

Enteric Methane ◽

In Vitro Rumen Fermentation

AbstractEnteric methane production contributes significantly to the greenhouse gas emission globally. Although, buffaloes are integral part of livestock production in Asian countries, contributing milk, meat and draft power, the contribution of enteric methane to environmental pollution attracts attention. The present study investigated the efficacy of garlic (Allium sativum) oil in reducing enteric methane production from buffaloes (Bubalus bubalis) by in vitro rumen fermentation. Garlic oil (GOL) was tested at four concentrations [0 (Control), 33.33 µl (GOL-1), 83.33 µl (GOL-2) and 166.66 µl (GOL-3) per litre of buffered rumen fluid] in 100-ml graduated glass syringes and incubated at 39℃ for 24 h for in vitro rumen fermentation study. Supplementation of GOL-1 increased (p < 0.05) total gas production in comparison with GOL-3; however, it remained comparable (p > 0.05) with control and GOL-2. Graded doses of garlic oil inclusions reduced (p < 0.001) methane concentration (%) in total gas and total methane production (ml/g DM), irrespective of concentrations. The feed degradability, volatile fatty acids and microbial biomass production (MBP) were not affected (p > 0.05) by GOL-1, but these tended to decrease in GOL-2 with marked reduction (p < 0.01) in GOL-3. The decrease (p < 0.01) in NH3–N concentration in fermentation fluid in the presence of garlic oil, irrespective of concentration, suggests reduced deamination by inhibiting rumen proteolytic bacterial population. The activities of ruminal fibrolytic enzymes (CMCase, xylanase, β-glucosidase, acetyl esterase) were not affected by lower dose (GOL-1) of garlic oil; however, reduction (p < 0.05) of these enzymes activity in rumen liquor was evident at higher doses (GOL-2 and GOL-3) of supplementation. This study shows positive impact of garlic oil supplementation at low dose (33.33 µl/l of rumen fluid) in reducing enteric methane production, thereby, abatement of environmental pollution without affecting feed digestibility.

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Utilization of dragon fruit waste by-products and non-protein nitrogen source: Effects on in vitro rumen fermentation, nutrients degradability and methane production

Livestock Science ◽

10.1016/j.livsci.2020.104386 ◽

2021 ◽

Vol 243 ◽

pp. 104386

Author(s):

M. Matra ◽

P. Totakul ◽

M. Wanapat

Keyword(s):

Nitrogen Source ◽

Methane Production ◽

Rumen Fermentation ◽

Protein Nitrogen ◽

Source Effects ◽

Dragon Fruit ◽

Fruit Waste ◽

By Products ◽

In Vitro Rumen Fermentation

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Effect of essential oils and aqueous extracts of plants on in vitro rumen fermentation and methane production

Journal of Animal Behaviour and Biometeorology ◽

10.31893/jabb.22010 ◽

2022 ◽

Vol 10 (1) ◽

pp. 1-9

Author(s):

Aarón A. Molho-Ortiz ◽

Atmir Romero-Pérez ◽

Efrén Ramírez-Bribiesca ◽

Claudia C. Márquez-Mota ◽

Francisco A. Castrejón-Pineda ◽

...

Keyword(s):

Essential Oils ◽

Methane Production ◽

Rumen Fermentation ◽

Aqueous Extracts ◽

In Vitro Rumen Fermentation

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Essential oils effect on rumen fermentation and biohydrogenation under in vitro conditions

Czech Journal of Animal Science ◽

10.17221/7708-cjas ◽

2014 ◽

Vol 59 (No. 10) ◽

pp. 450-459 ◽

Cited By ~ 11

Author(s):

M. Gunal ◽

A. Ishlak ◽

A.A. AbuGhazaleh ◽

W. Khattab

Keyword(s):

Fatty Acids ◽

Essential Oils ◽

Dose Level ◽

Volatile Fatty Acids ◽

Rumen Fluid ◽

Rumen Fermentation ◽

Buffer Solution ◽

Cinnamon Oil ◽

Tea Tree

The effects of adding essential oils (EO) at different levels (125, 250, 500 mg/l) on rumen fermentation and biohydrogenation were examined in a rumen batch culture study. Treatments were: control without EO (CON), control with anise oil (ANO), cedar wood oil (CWO), cinnamon oil (CNO), eucalyptus oil (EUO), and tea tree oil (TEO). Essential oils, each dissolved in 1 ml of ethanol, were added to the culture flask containing 40 ml of buffer solution, 2 ml of reduction solution, 10 ml of rumen fluid, 25 mg of soybean oil, and 0.5 g of the diet. After 24 h of incubation in a water batch at 39°C, three samples were collected from each flask and analyzed for ammonia-N, volatile fatty acids (VFA), and fatty acids (FA). Expect for CNO, the proportions of acetate, propionate, and acetate to propionate ratios were not affected (P > 0.05) by EO addition. Addition of CWO, CNO, and TEO reduced total VFA concentrations (P < 0.05) regardless of dose level. The ammonia-N concentration was greater in cultures incubated with EO regardless of dose level. Compared with the CON, the concentrations of C18:0 and trans C18:1 were reduced (P < 0.05) with EO addition regardless of dose level. Compared with the CON, the concentration of linoleic acid was greater (P < 0.05) when EO were added at 500 mg/l. EO tested in this study had no effects on VFA profile but significantly reduced the formation of biohydrogenation products (C18:0 and trans C18:1).

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Effects of Geraniol and Camphene on in Vitro Rumen Fermentation and Methane Production

Scientia Agriculturae Bohemica ◽

10.1515/sab-2017-0012 ◽

2017 ◽

Vol 48 (2) ◽

pp. 63-69

Author(s):

M. Joch ◽

V. Kudrna ◽

B. Hučko

Keyword(s):

Methane Emission ◽

Methane Production ◽

Dry Matter ◽

Volatile Fatty Acids ◽

Rumen Fluid ◽

Positive Control ◽

Rumen Fermentation ◽

Dry Matter Digestibility

AbstractThe objective of this study was to determine the effects of geraniol and camphene at three dosages (300, 600, and 900 mg l-1) on rumen microbial fermentation and methane emission in in vitro batch culture of rumen fluid supplied with a 60 : 40 forage : concentrate substrate (16.2% crude protein, 33.1% neutral detergent fibre). The ionophore antibiotic monensin (8 mg/l) was used as positive control. Compared to control, geraniol significantly (P < 0.05) reduced methane production with increasing doses, with reductions by 10.2, 66.9, and 97.9%. However, total volatile fatty acids (VFA) production and in vitro dry matter digestibility were also reduced (P < 0.05) by all doses of geraniol. Camphene demonstrated weak and unpromising effects on rumen fermentation. Camphene did not decrease (P > 0.05) methane production and slightly decreased (P < 0.05) VFA production. Due to the strong antimethanogenic effect of geraniol a careful selection of dose and combination with other antimethanogenic compounds may be effective in mitigating methane emission from ruminants. However, if a reduction in total VFA production and dry matter digestibility persisted in vivo, geraniol would have a negative effect on animal productivity.

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