Correlation Between Release of Individual Free Fatty Acids and Fatty Acid Composition of Adipose Tissue.

1966 ◽  
Vol 122 (4) ◽  
pp. 1276-1279 ◽  
Author(s):  
J. J. Spitzer ◽  
H. Nakamura ◽  
M. Gold ◽  
H. Altschuler ◽  
M. Lieberson
Keyword(s):  
Fatty Acids ◽  
Adipose Tissue ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Free Fatty Acids ◽  
Acid Composition

Removal and mobilization of individual free fatty acids in diabetic dogs

1962 ◽  
Vol 202 (5) ◽  
pp. 1002-1004 ◽  
Author(s):  
Martin Gold ◽  
Harvey I. Miller ◽  
John J. Spitzer
Keyword(s):  
Fatty Acids ◽  
Adipose Tissue ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Free Fatty Acids ◽  
Acid Composition ◽  
Total Fatty Acid Composition ◽  
Arterial Blood ◽  
Total Fatty Acids ◽  
Diabetic Dogs

Gas chromatographic analysis of individual free fatty acids showed elevated concentrations of the five major fatty acids in the arterial blood of acutely diabetic dogs. The percentage contributions of oleic and linoleic acids were also elevated. Insulin lowered both elevated concentrations and percentages. All major fatty acids were released into the epigastric vein from adipose tissue. Total fatty acid composition of adipose tissue was similar in normal and diabetic dogs. Adipose tissue free fatty acid composition was similar to that of blood and dissimilar to composition of total fatty acids. Myocardium failed to remove oleic acid in diabetic dogs unless insulin was administered. Consistent net removal by the liver of palmitoleic, stearic, and oleic acids occurred in diabetic animals.

Platelet Aggregation in Finnish Men and Its Relation to Fatty Acids in Platelets, Plasma and Adipose Tissue

1985 ◽  
Vol 54 (03) ◽  
pp. 563-569 ◽  
Author(s):  
M K Salo ◽  
E Vartiainen ◽  
P Puska ◽  
T Nikkari
Keyword(s):  
Fatty Acids ◽  
Adipose Tissue ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Platelet Aggregation ◽  
Acid Composition ◽  
Saturated Fatty Acids ◽  
Free Living ◽  
Ω3 Fatty Acids ◽  
Induced Aggregation

SummaryPlatelet aggregation and its relation to fatty acid composition of platelets, plasma and adipose tissue was determined in 196 randomly selected, free-living, 40-49-year-old men in two regions of Finland (east and southwest) with a nearly twofold difference in the IHD rate.There were no significant east-southwest differences in platelet aggregation induced with ADP, thrombin or epinephrine. ADP-induced platelet secondary aggregation showed significant negative associations with all C20-C22 ω3-fatty acids in platelets (r = -0.26 - -0.40) and with the platelet 20: 5ω3/20: 4ω 6 and ω3/ ω6 ratios, but significant positive correlations with the contents of 18:2 in adipose tissue (r = 0.20) and plasma triglycerides (TG) (r = 0.29). Epinephrine-induced aggregation correlated negatively with 20: 5ω 3 in plasma cholesteryl esters (CE) (r = -0.23) and TG (r = -0.29), and positively with the total percentage of saturated fatty acids in platelets (r = 0.33), but had no significant correlations with any of the ω6-fatty acids. Thrombin-induced aggregation correlated negatively with the ω3/6ω ratio in adipose tissue (r = -0.25) and the 20: 3ω6/20: 4ω 6 ratio in plasma CE (r = -0.27) and free fatty acids (FFA) (r = -0.23), and positively with adipose tissue 18:2 (r = 0.23) and 20:4ω6 (r = 0.22) in plasma phospholipids (PL).The percentages of prostanoid precursors in platelet lipids, i. e. 20: 3ω 6, 20: 4ω 6 and 20 :5ω 3, correlated best with the same fatty acids in plasma CE (r = 0.32 - 0.77) and PL (r = 0.28 - 0.74). Platelet 20: 5ω 3 had highly significant negative correlations with the percentage of 18:2 in adipose tissue and all plasma lipid fractions (r = -0.35 - -0.44).These results suggest that, among a free-living population, relatively small changes in the fatty acid composition of plasma and platelets may be reflected in significant differences in platelet aggregation, and that an increase in linoleate-rich vegetable fat in the diet may not affect platelet function favourably unless it is accompanied by an adequate supply of ω3 fatty acids.

scholarly journals Fatty acid composition of subcutaneous and intramuscular adipose tissue in east Balkan pigs

2015 ◽  
Vol 31 (4) ◽  
pp. 543-550 ◽  
Author(s):  
T. Popova ◽  
J. Nakev ◽  
Y. Marchev
Keyword(s):  
Fatty Acids ◽  
Adipose Tissue ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Polyunsaturated Fatty Acids ◽  
Acid Composition ◽  
Subcutaneous Fat ◽  
Intramuscular Fat ◽  
Longissimus Dorsi ◽  
Intramuscular Adipose Tissue

The aim of this study was to provide information on the fatty acid profile of different adipose depots - subcutaneous (upper and inner backfat layers) and intramuscular (m. Longissimus dorsi) in East Balkan pigs. The animals were reared in free-range conditions and slaughtered at an average live weight of 107?1.65kg. The results of the study showed that the various adipose tissues in pigs have different lipid metabolism and hence differ in their fatty acid composition. Intramuscular fat had significantly higher content of the saturated C16:0 and C18:0 (P<0.001), as well as the C16:1 (P<0.001) than the subcutaneous fat. In regards to the content of the polyunsaturated fatty acids, the latter displayed considerably higher content of both C18:2 and C18:3 (P<0.001) in comparison to the intramuscular fat in m. Longissimus dorsi. The differences between the subcutaneous and intramuscular adipose tissue in the individual fatty acids determined the similar trend of change in the total content of saturated and polyunsaturated fatty acids. Significant differences between the backfat layers were detected for C16:1, C18:0 and C18:3 (P<0.001). Stearic acid (C18:0) displayed higher content of the inner, while both C16:1 and C18:3 had higher proportion in the outer backfat layer in the East Balkan pigs. Except for C20:2, the long chain polyunsaturated n-6 and n-3 fatty acids had significantly higher proportions in the intramuscular fat, however no differences were determined between the two backfat layers.

UTILIZATION OF MILK AND ANIMAL FAT BY MICROORGANISMS – POTENTIAL PRODUCERS OF LIPASES AND ESTERASES

2020 ◽  
pp. 44-45
Author(s):  
D. Ivasenko ◽  
P. Bukhtiyarova ◽  
D. Antsiferov ◽  
Y. Frank
Keyword(s):  
Fatty Acids ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Free Fatty Acids ◽  
Acid Composition ◽  
Liquid Culture ◽  
Culture Media ◽  
Bacterial Strains ◽  
Animal Fat ◽  
Pure Cultures

Analysis of fatty acid composition in liquid culture media after lipophilic bacterial strains cultivation was carried out. Pure cultures were earlierisolated from fat-containing wastes and cultivated on media with diverse fat sources. It was shown that microorganisms hydrolyze animal and milk fats to free fatty acids.

scholarly journals Lipid Profile, Lipase Bioactivity, and Lipophilic Antioxidant Content in High Pressure Processed Donor Human Milk

Nutrients ◽  
2019 ◽  
Vol 11 (9) ◽  
pp. 1972 ◽  
Author(s):  
Wesolowska ◽  
Brys ◽  
Barbarska ◽  
Strom ◽  
Szymanska-Majchrzak ◽  
...  
Keyword(s):  
Fatty Acids ◽  
High Pressure ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Human Milk ◽  
Free Fatty Acids ◽  
Acid Composition ◽  
Induction Time ◽  
Milk Fat ◽  
Human Milk Fat

Human milk fat plays an essential role as the source of energy and cell function regulator; therefore, the preservation of unique human milk donors’ lipid composition is of fundamental importance. To compare the effects of high pressure processing (HPP) and holder pasteurization on lipidome, human milk was processed at 62.5 °C for 30 min and at five variants of HPP from 450 MPa to 600 MPa, respectively. Lipase activity was estimated with QuantiChrom™ assay. Fatty acid composition was determined with the gas chromatographic technique, and free fatty acids content by titration with 0.1 M KOH. The positional distribution of fatty acid in triacylglycerols was performed. The oxidative induction time was obtained from the pressure differential scanning calorimetry. Carotenoids in human milk were measured by liquid chromatography. Bile salt stimulated lipase was completely eliminated by holder pasteurization, decreased at 600 MPa, and remained intact at 200 + 400 MPa; 450 MPa. The fatty acid composition and structure of human milk fat triacylglycerols were unchanged. The lipids of human milk after holder pasteurization had the lowest content of free fatty acids and the shortest induction time compared with samples after HPP. HPP slightly changed the β-carotene and lycopene levels, whereas the lutein level was decreased by 40.0% up to 60.2%, compared with 15.8% after the holder pasteurization.

Effect of increasing stearoyl coenzyme A desaturase mRNA concentrations using forage and concentrate diets on the fatty acid composition of ovine adipose tissue

2002 ◽  
Vol 2002 ◽  
pp. 206-206 ◽  
Author(s):  
Z.C.T.R. Daniel ◽  
R.J. Wynn ◽  
A.M. Salter ◽  
P.J. Buttery
Keyword(s):  
Fatty Acids ◽  
Adipose Tissue ◽  
Fatty Acid Composition ◽  
Oleic Acid ◽  
Fatty Acid ◽  
Stearic Acid ◽  
Acid Composition ◽  
Coenzyme A ◽  
Saturated Fatty Acids ◽  
Mrna Levels

Compared to meat from other animals lamb contains high levels of saturated fat, particularly stearic acid which comprises 18% of the total fatty acids (Enser et al, 1996). This stearic acid can be desaturated in the tissue by stearoyl coenzyme A desaturase (SCD) to produce oleic acid. In sheep SCD is produced from a single gene and the levels of SCD mRNA in the tissue correlate well with oleic acid (Ward et al, 1998, Barber et al, 2000) suggesting that an upregulation of SCD activity may increase the relative proportions of unsaturated and saturated fatty acids and so significantly improve the nutritional quality of sheep meat. Our recent studies have shown that insulin increases SCD mRNA levels and monounsaturated fatty acid synthesis in cultured ovine adipose tissue explants (Daniel et al, 2001). The present study was designed to investigate whether feeding a diet believed to manipulate SCD mRNA concentrations would significantly alter the fatty acid composition of lamb.

scholarly journals Influence of Diet on Fatty-Acid Composition of Depot Fat in Western Sandpipers (Calidris Mauri)

The Auk ◽  
2003 ◽  
Vol 120 (2) ◽  
pp. 337-345 ◽  
Author(s):  
Oliver Egeler ◽  
Dana Seaman ◽  
Tony D. Williams
Keyword(s):  
Fatty Acids ◽  
Adipose Tissue ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Acid Composition ◽  
De Novo ◽  
Calidris Mauri ◽  
Total Percentage ◽  
Western Sandpipers ◽  
Experimental Treatments

Abstract Western Sandpipers (Calidris mauri) have been previously shown to undergo seasonal changes in the fatty acid composition of their fat stores, even though they do not show the marked seasonal variation in diet common to many migratory passerines. We investigated the effect of dietary fatty acid composition on the fatty acid composition of adipose tissue in captive Western Sandpipers by feeding birds experimental diets with different fatty acid composition. In addition, we determined the effect of total percentage of fat content of the diet (5 vs. 10%) on fatty acid composition of depot fat. Birds maintained normal body mass (24–27 g) throughout all experimental treatments. Most adipose fatty acids were sensitive to dietary manipulation to some extent. Changes in fatty acid composition of the diet had the largest effect on adipose tissue composition for the essential polyunsaturated fatty acid linoleate (18:2), whereas it had the least effect for the monounsaturated fatty acid oleate (18:1). The saturated fatty acid palmitate (16:0) demonstrated an intermediate capacity to alter fatty acid composition of adipose tissue. Total amount of fat in the diet did not influence the effect of diet on fatty acid deposition. Results of dietary manipulations in this study suggest that diet does explain some of the variation in fatty acid composition observed during migration in Western Sandpipers, but that certain fatty acids can be modulated independently of diet (probably through de novo synthesis, postabsorption modification, or both).

The effects of fat source and breed on the fatty acid composition of lamb muscle and adipose tissue

1999 ◽  
Vol 1999 ◽  
pp. 115-115 ◽  
Author(s):  
A.M. Wachira ◽  
L.A. Sinclair ◽  
R.G. Wilkinson ◽  
G. Demirel ◽  
M. Enser ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Adipose Tissue ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Vitamin E ◽  
Fish Oil ◽  
Acid Composition ◽  
Previous Experiment ◽  
Dietary Pufa ◽  
Long Chain

The benefits of long chain polyunsaturated fatty acids (PUFA) to human health, especially those of the n-3 series are now widely recognised. In a previous experiment (Wachira et al. 1998) supplementing diets with whole linseed or fish oil increased n-3 fatty acid levels in lamb muscle. To raise these further the whole linseed can be treated with formaldehyde to increase protection in the rumen. Dietary antioxidants such as vitamin E can control lipid oxidation but information on their effects on lamb performance and fatty acid composition is limited. The current experiments investigated the effects of different dietary PUFA sources and vitamin E levels on growth and fatty acid composition in two sheep breeds. Detailed results of the effects of vitamin E are presented in the accompanying abstract by Enser et al.

Effects of supplementation with safflower oil on voluntary feed intake and tissue fatty acid composition of steers fed low quality hay ad libitum

1993 ◽  
Vol 33 (6) ◽  
pp. 693 ◽  
Author(s):  
JC O'Kelly ◽  
WG Spiers
Keyword(s):  
Fatty Acids ◽  
Adipose Tissue ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Acid Composition ◽  
Feed Intake ◽  
Blood Platelets ◽  
Safflower Oil ◽  
Body Protein ◽  
Voluntary Feed Intake

The effect of safflower oil supplement (200 mL/day) on voluntary feed intake and fatty acid composition of plasma, blood platelets, subcutaneous adipose tissue, and M. longissimus dorsi muscle was studied in Brahman steers consuming a low quality hay. The initial mean liveweight (LW) of the steers was 197 kg. Mean LW gain over 50 days was 20 kg for all steers, but voluntary feed intake (5.63 kg/day) was reduced by 1.02 kg/day with safflower oil. Supplementation increased the plasma concentrations of unsaturated fatty acids, indicating a less complete hydrogenation of dietary safflower oil than of hay by rumen microorganisms. Safflower oil altered the fatty acid (FA) profiles of platelet and muscle phospholipids. with increases in the proportions of monounsaturated acids (MFA) and polyunsaturated fatty acids (PFA) and associated decreases in the proportions of saturated fatty acids (SFA). The neutral lipids of adipose tissue consisted of 60% SFA and 38% MFA in steers fed hay alone, compared with 53% SFA and 45% MFA in supplemented steers. Safflower oil had no effect on the total fat content of muscle; the concentration of PFA was not significantly altered, but SFA decreased 17% in parallel with a 23% increase in MFA. The results support proposals that dietary oil supplements may be used to reduce forage intake during dry season or drought, to conserve body protein and to modify the FA composition of meat phospholipids which may, in turn, influence meat quality of ruminants consuming low quality roughage diets.

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