Fusarium oxysporum strains from wilting potato plants : Potential causal agents of dry rot disease in potato tubers

2021 ◽  
Vol 22 (Special) ◽  
Genes ◽  
2019 ◽  
Vol 10 (9) ◽  
pp. 658 ◽  
Author(s):  
Khalid ◽  
Lv ◽  
Naeem ◽  
Mehmood ◽  
Shaheen ◽  
...  

Fusarium oxysporum is the most important pathogen of potatoes which causes post-harvest destructive losses and deteriorates the market value of potato tubers worldwide. Here, F. oxysporum was used as a host pathogen model system and it was revealed that autophagy plays a vital role as a regulator in the morphology, cellular growth, development, as well as the pathogenicity of F. oxysporum. Previous studies based upon identification of the gene responsible for encoding the autophagy pathway components from F. oxysporum have shown putative orthologs of 16 core autophagy related-ATG genes of yeast in the genome database which were autophagy-related and comprised of ubiquitin-like protein atg3. This study elucidates the molecular mechanism of the autophagy-related gene Foatg3 in F. oxysporum. A deletion (∆) mutants of F. oxysporum (Foatg3∆) was generated to evaluate nuclear dynamics. As compared to wild type and Foatg3 overexpression (OE) strains, Foatg3∆ strains failed to show positive MDC (monodansylcadaverine) staining which revealed that Foatg3 is compulsory for autophagy in F. oxysporum. A significant reduction in conidiation and hyphal growth was shown by the Foatg3∆ strains resulting in loss of virulence on potato tubers. The hyphae of Foatg3∆ mutants contained two or more nuclei within one hyphal compartment while wild type hyphae were composed of uninucleate hyphal compartments. Our findings reveal that the vital significance of Foatg3 as a key target in controlling the dry rot disease in root crops and potato tubers at the postharvest stage has immense potential of disease control and yield enhancement.


Genes ◽  
2019 ◽  
Vol 10 (5) ◽  
pp. 365 ◽  
Author(s):  
A. Rehman Khalid ◽  
Shumin Zhang ◽  
Xiumei Luo ◽  
Khalid Mehmood ◽  
Junaid Rahim ◽  
...  

Autophagy is a universal catabolic process preserved in eukaryotes from yeast to plants and mammals. The main purpose of autophagy is to degrade cytoplasmic materials within the lysosome/vacuole lumen and generate an internal nutrient pool that is recycled back to the cytosol during nutrient stress. Here, Fusarium oxysporum was utilized as a model organism, and we found that autophagy assumes an imperative job in affecting the morphology, development, improvement and pathogenicity of F. oxysporum. The search of autophagy pathway components from the F. oxysporum genome database recognized putative orthologs of 16 core autophagy-related (ATG) genes of yeast, which additionally incorporate the ubiquitin-like protein atg22. Present study elucidates the unreported role of Foatg22 in formation of autophagosomes. The deletion mutant of Foatg22 did not demonstrate positive monodansylcadaverine (MDC) staining, which exposed that Foatg22 is required for autophagy in F. oxysporum. Moreover, the ∆Foatg22 strains exhibited a decrease in hyphal development and conidiation, and reduction in pathogenicity on potato tubers and leaves of potato plant. The hyphae of ∆Foatg22 mutants were less dense when contrasted with wild-type (WT) and overexpression (OE) mutants. Our perceptions demonstrated that Foatg22 might be a key regulator for the control of dry rot disease in tuber and root crops during postharvest stage.


Plant Disease ◽  
2012 ◽  
Vol 96 (12) ◽  
pp. 1767-1774 ◽  
Author(s):  
E. Gachango ◽  
L. E. Hanson ◽  
A. Rojas ◽  
J. J. Hao ◽  
W. W. Kirk

A survey of seed potato tubers in Michigan seed production storage facilities was carried out during 2009 and 2010. Fusarium spp. associated with tuber dry rot symptoms were identified to species and tested for sensitivity to difenoconazole, fludioxonil, and thiabendazole. Symptomatic tubers (n = 370) were collected from a total of 51 seed lots, from which 228 isolates of Fusarium were recovered and identified to 11 species. Fusarium oxysporum was the most commonly isolated species (30.3%), followed by F. equiseti (19.3%). F. sambucinum and F. avenaceum were third most prevalent (each at 13.6%). Less prevalent species (each at 4 to 10%) included F. cerealis, F. solani, and F. acuminatum; and species present at ≤3% included F. sporotrichioides, F. torulosum, F. tricinctum, and F. graminearum. Representative isolates of all species were pathogenic when inoculated onto seed tubers (‘Dark Red Norland’). Isolates of F. sambucinum were the most virulent. All 228 isolates of Fusarium were sensitive to difenoconazole (effective fungicide concentration that caused 50% inhibition of mycelial growth [EC50] < 5 mg/liter). Insensitivity to fludioxonil (EC50 > 100 mg/liter) was detected only for F. sambucinum and F. oxysporum isolates at 8.9 and 20.4%, respectively. All isolates were sensitive to thiabendazole (EC50 < 5 mg/liter), except for those of F. sambucinum (EC50 > 100 mg/liter). Therefore, knowledge of what Fusarium spp. are present in seed potato storage facilities in Michigan may be important if using fludioxonil or thiabendazole for seed piece treatment but not when using difenoconazole.


2018 ◽  
Vol 7 (2) ◽  
pp. 53-61
Author(s):  
Mostafa H. Mostafa

Potato tubers, under storage conditions, suffer from Fusarium dry rot disease caused by Fusarium spp. leading to devastating losses of stored tubers.  Newly harvested tubers are resistance toward Fusarium infection, but disease becomes more vigorous by increasing storage period.  Inoculation of potato tuber slices of Desiree cv. (highly susceptible) or Spunta cv. (highly resistant) either by a weak pathogen (F. moniliforme) or severe pathogen (F. solani) and tracking accumulation of phytoalexin rishitin in inoculated tissues at different intervals from inoculation revealed that Desiree cv. accumulated high amounts of rishitin in a very short time in comparison to another three tested situations. Dipping of potato tuber slices of both cvs. in different concentrations of the known antibiotic chloramphenicol prior to inoculation with a weak pathogen (F. moniliforme) led to increased disease severity associated with accumulation huge amounts of rishitin in inoculated tissues. The correlation coefficient between disease severity and rishitin production was +0.83.  Moreover, the reaction of spunta cv. toward inoculation by a weak pathogen was changed from the state of resistance to very severe susceptibility.  Since chloramphenicol affects protein synthesis on 70S ribosome’s, it was postulated that it prevents synthesis of factor(s) take a part in cell death during infection. Mitochondria extracted from potato tuber tissues secret compound(s) decreased rishitin synthesis in potato tuber slices treated with mycelia extract of F. solani. Treatment of isolated mitochondria with this antibiotic led to the elimination of this factor(s) from their secretions. Results obtained revealed that the elicitation of phytoalexins may consider one of pathogenicity factor of such system.


Author(s):  
Nadia Azil ◽  
Emil Stefańczyk ◽  
Sylwester Sobkowiak ◽  
Saliha Chihat ◽  
Houda Boureghda ◽  
...  

AbstractFusarium is one of the most important genera of phytopathogenic fungi, causing potato wilt in the field and potato tuber dry rot during storage. The objectives of this study were to identify Fusarium species associated with both potato diseases in different growing regions in Algeria, and to assess their pathogenicity. Among the 152 isolates collected from symptomatic potato plants and tubers in different provinces in Algeria, 13 species of Fusarium and Neocosmospora were identified. Among these three species were isolated only from plants showing symptoms of Fusarium potato wilt (F. oxysporum, F. venenatum, Neocosmospora solani). Two species (F. culmorum, N. tonkinensis) and an isolate of Neocosmospora sp. were found exclusively in tubers with potato dry rot and the remaining ones (F. redolens, F. cf. tricinctum, F. sambucinum, F. cf. incarnatum-equiseti, F. nygamai, F. brachygibbosum and N. falciformis) were associated with both sample types. Fusarium sambucinum was the most frequent species (52.6% of isolates). Fusarium oxysporum and F. nygamai isolates were the most aggressive in the potato wilt pathogenicity test, and F. sambucinum isolates were the most aggressive in the potato tuber pathogenicity test. This is the first study identifying and characterizing potato dry rot and potato wilt pathogens in Algeria.


Author(s):  
F. S. Melnichuk ◽  
S. A. Alekseeva ◽  
O. V. Hordiienko

The Colorado potato beetles and aphids are especially harmful pests for potato crops in the conditions of Kyiv region. So, on the 10th day after mass rebirth of the Colorado potato beetle larvae, potato plants on untreated by insecticide plots were completely destroyed by this phytophagus. Preplanting insecticide treatment of potato tubers provided high effectiveness against the Colorado potato beetle prior to the mass rebirth and development of larvae and reduced their density of population and harmfulness. The highest efficacy (93.2-95.2%) against these pests was noted in variants with Prestige, 290 FS, Emesto Quantum 273.5 FS and Celest Top 312.5 FS. Common scab, dry rot and Rhizoctonia solani were prevailed among the diseases of potato tubers. The greatest effectiveness against the common scab was provided by Prestige, 290 FS. Thus, the infestation of potato tubers by common scab in the variant with application of the Prestige, 290 FS was almost twice lower compared to control. Preparation Celest Top 312.5 FS provided a reduction of dry rot in almost 3 times, and Emesto Quantum 273.5 FS leads to reduction of infestation by Rhizoctonia solani in 4 times, respectively. The treatment of tubers is a reliable protection of potato plants against damage by the Colorado potato beetles and lesion diseases, that made it possible to obtain tuber yields at a level of 24.4-27.4 t per ha. However, preplanting treatment of planting material with insecticides is not always able to provide the protection of potato seedlings against damage by Colorado beetles. The mass reproduction of these pests occurs after planting of tubers and emerging of stalks at high air temperatures. Therefore, it is necessary to provide additionally spraying of plants. Typically, the insecticides used in recommended rates have a long period of protective action, which eliminates the need for further treatments on vegetative plants. The anti-resistant effect of this treatment is associated with the use of the neonicotinoids class insecticides (eg, imidacloprid, thiamethoxam, clothianidin), that have different mechanism of action compared to pyrethroids. Under such conditions, it is important to search, investigate and introduce modern, effective insecticides into agricultural production. For this purpose the research on the preparations from separate classes of chemical compounds with various toxic properties was conducted. The most effective against the larvae of Colorado potato beetles were insecticides Confidor, 20% SC, Calypso, 48% SC and Engeo 24.7% SC when spraying potato plantings. Duration of their protection was prolonged for two weeks after the application of insecticides. The maximum technical efficiency was reached as 99.5% (Confidor, 20% SC), 99.2% (Calypso, 48% SC) and 99.3% (Engeo 24.7% SC) on the third day after application. Other insecticides such as Mospilan, 20% SP and Actara, 24% WG (96.2-97.0%) provided somewhat lower the mortality rate of these phytophages. The highest and prolonged effect against aphids was obtained by spraying potato plantings with the preparations Engeo 24.7% SC and Confidor, 20% SC, the efficiency of which reached 98.2% and 93.1%, that decreased the number of aphids in almost 60 times, compared with the control. Spraying potato plantings with insecticides according to the recommended rates of application allows obtaining the yield of tubers at the level of 25,6-28,2 t per ha.    


2019 ◽  
Vol 9 (2) ◽  
pp. 91
Author(s):  
Ghea Dotulong ◽  
Stella Umboh ◽  
Johanis Pelealu

Uji Toksisitas Beberapa Fungisida Nabati terhadap Penyakit Layu Fusarium (Fusarium oxysporum) pada Tanaman Kentang (Solanum tuberosum L.) secara In Vitro (Toxicity Test of several Biofungicides in controlling Fusarium wilt (Fusarium oxysporum) in Potato Plants (Solanum tuberosum L.) by In Vitro) Ghea Dotulong1*), Stella Umboh1), Johanis Pelealu1), 1) Program Studi Biologi, FMIPA Universitas Sam Ratulangi, Manado 95115*Email korespondensi: [email protected] Diterima 9 Juli 2019, diterima untuk dipublikasi 10 Agustus 2019 Abstrak Tanaman kentang (Solanum tuberosum L.) adalah salah satu tanaman hortikultura yang sering mengalami penurunan dari segi produksi dan produktivitasnya, akibat adanya serangan penyakit layu yang salah satunya disebabkan oleh Fusarium oxysporum. Tujuan penelitian ini adalah mengidentifikasi toksisitas beberapa fungisida nabati dalam mengendalikan penyakit Layu Fusarium (F. oxysporum) pada tanaman kentang (Solanum tuberosum L.) secara In Vitro. Metode Penelitian yang digunakan yaitu metode umpan beracun. Data dianalisis dengan Rancangan Acak Lengkap (RAL) dengan Analisis Varian (ANAVA) yang dilanjutkan dengan menggunakan metode BNT (Beda Nyata Terkecil). Hasil Penelitian, diperoleh nilai toksisitas fungisida nabati tertinggi yaitu pada ekstrak daun sirsak dengan nilai HR (69,44%), kategori berpengaruh, ditandai dengan diameter koloni 2,75 cm (100ppm) dan yang terendah toksisitasnya yaitu pada ekstrak daun jeruk purut dengan nilai HR (49,81%), kategori cukup berpengaruh ditandai dengan diameter koloni 3,75 cm (25ppm). Semakin tinggi konsentrasi yang diujikan maka semakin tinggi toksisitas dari fungisida nabati yang diberikan.Kata Kunci: fungisida nabati, Fusarium oxysporum, tanaman kentang, In Vitro Abstract Potato plants (Solanum tuberosum L.) is one of the horticulture plants which often decreases in terms of production and productivity, due to the attack of wilt, one of which is caused by Fusarium oxysporum. The purpose of this study was to determine the toxicity of several biofungicides in controlling Fusarium wilt (F. oxysporum) in potato plants (Solanum tuberosum L.) in Vitro. The research method used was the In Vitro method with the poison bait method. Data were analyzed by Completely Randomized Design with Variant Analysis (ANAVA), followed by the BNT method. The results showed that the highest biofungicide toxicity value was soursop leaf extract with HR values (69.44%), influential categories, characterized by colony diameter 2.75 cm (100ppm) and the lowest toxicity, namely in kaffir lime leaf extract with a value of HR (49.81%), quite influential category was characterized by colony diameter of 3.75 cm (25ppm). The higher the concentration tested, the higher the toxicity of the biofungicide given.Keywords: biofungicides, Fusarium oxysporum, Potato Plants, In Vitro.


2021 ◽  
Vol 113 ◽  
pp. 101601
Author(s):  
Jie Ren ◽  
Jie Tong ◽  
Peihua Li ◽  
Xiaoqing Huang ◽  
Pan Dong ◽  
...  

1986 ◽  
Vol 26 (6) ◽  
pp. 745 ◽  
Author(s):  
PA Taylor ◽  
SP Flett ◽  
RFde Boer ◽  
D Marshall

The period of susceptibility of potato tubers to powdery scab (Spongospora subterranea) was studied by inoculating potato plants with spores, or by watering plants in infested soil, at different stages of plant development in greenhouse conditions. Maximum susceptibility began about 1 week before the stage when 50% of stolons had swollen to at least 5-mm diameter (tuber set), and ended 3-4 weeks later. With holding irrigation water during this period reduced the severity of powdery scab by 65-75% in field experiments in 1981-82 and 1982-83, but had no apparent effect on disease severity in 3 out of 6 large-scale field trials during 1984-85.


2020 ◽  
Vol 14 (1) ◽  
pp. 339-344
Author(s):  
Sabah R. Mohammed ◽  
Ivan D. Eskov ◽  
Elsayed M. Zeitar

Background: Fusarium dry rot disease caused by Fusarium sambucinum Fuckel (F. sambucinum) can infect the potato tubers in the field and during storage. Yield losses by F. sambucinum reach 60%. Traditional methods to control Fusarium dry rot are fungicides application, which led to developing many isolates resistant to these fungicides. Objective: The aim of this study is to evaluate the effect of calcium chloride (CaCl2) and chitosan, alone or in combination, on plant development, tuber yield, and Fusarium dry rot disease incidence under field conditions. Methods: Soil inoculated with F. sambucinum before planting. We treated the seed tubers with CaCl2 (0.5 or 1%), chitosan 0.5%, or both. The foliage was sprayed twice with CaCl2 (0.5 or 1%), 0.1% chitosan, or both. During the vegetation period, growth parameters, such as germination (%), plant height (cm), and branches number per plant, were measured. At harvest, we calculated the total and the marketable number of tubers and tuber yield. In addition, during storage, we assessed the incidence of Fusarium dry rot disease on tubers. Results: Results revealed that combined pre-planting application with 1% CaCl2 and 0.5% chitosan with 2 hours intervals, then spraying foliar with 1% CaCl2 and 0.1% chitosan twice with ten days intervals starting at 40 days after planting resulted in: a) increasing the germination, enhancing the growth parameters such as plant height and branches number per plant; b) enhancing the marketable tuber yield by 75.2 and 97.6% in Sante and Kolobok varieties, respectively; c) reducing Fusarium dry rot disease incidence by 61.9-72.7%. Conclusion: The work highlighted that the combined pre-planting and foliar application of CaCl2 and chitosan might be recommended for potato producers to reduce the incidence of Fusarium dry rot disease and augment yields.


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