Pigments, Polyphenols and Antioxidant Activity of Leaf Extracts from Four Wild Rose Species Grown in Sicily

Leaf methanolic extracts of four wild Rose species (Rosa canina L., R. corymbifera Borkh., R. micrantha Borrer ex Sm and R. sempervirens L.) grown in Sicily were analysed in order to evaluate differences among indigenous roses which could be used such as source of bioactive compounds and natural antioxidants. Leaves were harvested during two different periods (June and October) and their morphological characteristics were described. Leaf content of chlorophylls, total anthocyanins, total flavonoids, total polyphenols (Folin-Ciocalteu method) and antioxidant activity (DPPH method) were determined. Results showed that the contents of chlorophylls and carotenoids were higher in leaves of all species harvested in June than in those harvested in October. On the contrary, total anthocyanins of R. micrantha and R. corymbifera leaves were higher in October. Total polyphenol content was higher in leaves of all species were harvested in October. No difference in total flavonoids content was recorded between the two harvest periods but only among the four species. Antioxidant activity was generally high in leaf extracts of all species in both periods and, particularly, in R. micrantha leaves harvested in June. These outcomes seem to support the assessment that leaves harvested from some Sicilian wild roses may be used as promising source of healthy compounds and that their content varies with the species and harvest period.

Download Full-text

Phenolic Contents and Antioxidant Potential ofCrataegusFruits Grown in Tunisia as Determined by DPPH, FRAP, andβ-Carotene/Linoleic Acid Assay

Journal of Chemistry ◽

10.1155/2013/378264 ◽

2013 ◽

Vol 2013 ◽

pp. 1-6 ◽

Cited By ~ 9

Author(s):

Farouk Mraihi ◽

Mohamed Journi ◽

Jamila Kalthoum Chérif ◽

Munevver Sokmen ◽

Atalay Sokmen ◽

...

Keyword(s):

Antioxidant Activity ◽

Linoleic Acid ◽

Natural Antioxidants ◽

Total Phenolic ◽

Frap Assay ◽

Phenolic Contents ◽

Dpph Method ◽

Total Anthocyanins ◽

Linoleic Acid Assay

Crataegusfruit is one of most important fruits in Tunisian flora. Some fruits of this genus are edible. This study was undertaken in order to examine the benefits of these fruits in human health and their composition of antioxidants including total polyphenol, flavonoids, proanthocyanidins content, and total anthocyanins. The antioxidative properties of the ultrasonic methanolic extract were assessed by different in vitro methods such as the FRAP, DPPH, andβ-carotene/linoleic acid assay. We concluded that peel fraction of red fruits possessed relatively high antioxidant activity and might be a rich source of natural antioxidants in comparison with the pulp and seed fruit extract. The results also showed that hawthorn yellow fruit presents lower amounts of phenolic content, absence of anthocyanins, and less antioxidant capacity. Most of peel and seed fractions were stronger than the pulp fractions in antioxidant activity based on their DPPH IC50, FRAP values, and results ofβ-carotene/linoleic acid. The total phenolic compounds contents were also highly correlated with the DPPH method and the FRAP assay.

Download Full-text

Antioxidant Activity of Some Edible Flowers Water Extracts from Bulgaria

Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca Food Science and Technology ◽

10.15835/buasvmcn-fst:2019.0025 ◽

2020 ◽

Vol 77 (1) ◽

pp. 54

Author(s):

Dorina PETKOVA ◽

Dasha MIHAYLOVA ◽

Petko DENEV ◽

Albert KRASTANOV

Keyword(s):

Antioxidant Activity ◽

Antioxidant Properties ◽

Natural Antioxidants ◽

Rosa Damascena ◽

Total Flavonoids ◽

Alternative Source ◽

Plant Materials ◽

Total Flavonoids Content ◽

Viola Tricolor ◽

Calendula Officinalis L

Recently, there has been a considerable interest in finding natural antioxidants from plant materials to replace the synthetic ones. The edible flowers represent a valuable alternative source of bioactive compounds and have been used as food and herbs with increasing interest. The antioxidant properties of 7 edible flowers of the South Bulgaria, including Viola tricolor L., Cucurbita pepo L., Sambucus nigra L., Calendula officinalis L., Hibiscus rosa-sinensis L., Rosa damascena Mill., and Allium ursinum L. were evaluated. The contents of flower chemicals, such as total phenolics and total flavonoids content, were determined as well. By comparing decoction and infusion as methods of extraction, the decoction ones revealed to be the most appropriate in respect of the evaluated compounds. The results showed that the highest antioxidant activity was found in the Rosa damascena and Viola tricolor ones. The established total polyphenol content and total flavonoids in the decocts of Rosa damascena and Viola tricolor were 56.66 ± 0.48 and 135.82 ± 1.50 mg GAE/g dw and 28.60 ± 0.43 and 15.87± 0.52 mg QЕ/g dw, respectively. The present research extends the traditional knowledge and revealed an opportunity to obtain biological active substances of the nature and edible flowers in particular.

Download Full-text

Evaluation of phenolic compounds and antioxidant activity in some edible flowers

Open Agriculture ◽

10.1515/opag-2020-0087 ◽

2020 ◽

Vol 5 (1) ◽

pp. 857-870

Author(s):

Fernando Gonçalves ◽

João Carlos Gonçalves ◽

Ana Cristina Ferrão ◽

Paula Correia ◽

Raquel P. F. Guiné

Keyword(s):

Antioxidant Activity ◽

Total Phenolics ◽

Total Phenolic ◽

Potential Health ◽

Phenolic Contents ◽

Spectrophotometric Methods ◽

Potential Source ◽

Successive Extraction ◽

Dpph Method ◽

Total Anthocyanins

AbstractRecently, edible flowers (EF) have aroused increased interest because of their aesthetic properties as well as potential health benefits related to the occurrence of some bioactive compounds. Therefore, the aim of this work was to evaluate the total phenolics, anthocyanins, flavonoids, and antioxidant activity (AOA) (following DPPH and ABTS methods) in eleven EF. The samples were subjected to three successive extraction steps using methanol, and these extracts were then analysed for the aforementioned properties using spectrophotometric methods. The obtained extracts were used for the quantification of phenolic composition and AOA. The results indicated that, among the flowers analysed in this study, red rose, pink rose, and red carnation possessed the highest total phenolic contents (27.53, 23.30, and 18.17 mg g−1 gallic acid equivalents, respectively), total anthocyanins (3.07, 1.97, and 4.47 mg g−1 catechin equivalents [CE], respectively), and AOA (12.07, 15.77, and 12.93 mg g−1 TE, respectively, as given by the DPPH method or 8.23, 9.27 and 8.00 mg g−1 TE, respectively, as given by the ABTS method). The flowers with highest flavonoids contents were red carnation, Mexican marigold, and pink rose (17.50, 16.90, and 16.57 mg g−1 CE, respectively). Cluster analysis grouped the analysed flowers into two groups, those richest in phenolics with AOA and those not so rich. Finally, some important correlations were observed between the total phenolics and the AOA. In conclusion, these flowers could represent a potential source of natural compounds with antioxidant capacity.

Download Full-text

FRACTIONATION OF THE N-HEXANE EXTRACT OF GARCINIA BANCANA MIQ. (MANGGIS HUTAN) LEAVES AND ITS ANTIOXIDANT ACTIVITY BASED ON 1,1-DIPHENYL-2- PICRYLHYDRAZYL AND FERRIC REDUCING ANTIOXIDANT POWER ASSAYS

International Journal of Applied Pharmaceutics ◽

10.22159/ijap.2018.v10s1.90 ◽

2018 ◽

Vol 10 (1) ◽

pp. 407

Author(s):

Dewi Kumala Putri ◽

Berna Elya ◽

Nuraini Puspitasari

Keyword(s):

Antioxidant Activity ◽

Antioxidant Activities ◽

Hexane Extract ◽

Phytochemical Screening ◽

Active Fraction ◽

Leaf Extracts ◽

Antioxidant Power ◽

Dpph Method ◽

Ferric Reducing Antioxidant Power

Objective: To assess the antioxidant activity from another part of the plant, in this study, leaf extracts in n-hexane were fractionated.Methods: Ten fractions were obtained and tested in vitro for antioxidant activity using two methods, 1,1-diphenyl-2-picrylhydrazyl (DPPH) and ferricreducing antioxidant power (FRAP), to identify the most active fraction.Results: The IC50 of the most active fraction was 36.24 μg/mL using the DPPH method, and the EC50 was 39.54 μg/mL using the FRAP method. Themost active fraction was also shown to contain terpenoids.Conclusion: The most active fraction of an n-hexane extract of the leaves of Gacinia bancana Miq., which was tested by both DPPH and FRAP methodshad antioxidant activities with IC50 and EC50 values of 36.2482 μg/mL and 39.5442 μg/mL, respectively. Phytochemical screening showed that activefraction contains terpenoids.

Download Full-text

Determination of Chemical Constituents and Antioxidant Activities of Leaves and Stems from Jatropha cinerea (Ortega) Müll. Arg and Jatropha cordata (Ortega) Müll. Arg

Plants ◽

10.3390/plants10020212 ◽

2021 ◽

Vol 10 (2) ◽

pp. 212

Author(s):

Yeimi Cecilia Vega-Ruiz ◽

Corina Hayano-Kanashiro ◽

Nohemí Gámez-Meza ◽

Luis Angel Medina-Juárez

Keyword(s):

Antioxidant Activity ◽

Antioxidant Activities ◽

Biological Effects ◽

Chemical Constituents ◽

Weather Conditions ◽

Antioxidant Compounds ◽

Leaf Extracts ◽

Methanolic Extracts ◽

Northwestern Region ◽

High Concentrations

Jatropha species have been shown to be an important source of secondary metabolites with different biological effects. Jatropha cinerea (Ortega) Müll. Arg and Jatropha cordata (Ortega) Müll. Arg are distributed in the Northwestern region of Mexico, are adapted to extreme weather conditions and are widely used (stems, leaves, and sap) in traditional medicine. The aim of the present study was to carry out the phytochemical characterization and the evaluation of the antioxidant activity in methanolic extracts of stems and leaves from J. cinerea and J. cordata. The compounds present in the extracts of both species were characterized by ESI-IT-MS/MS and quantified by HPLC-DAD. The results showed that the stem extracts of both species are rich in phenolic acids, while the leaf extracts are rich in flavonoids. Some of the main compounds found were gallic acid, gentisic acid, 3,4-Dihydroxybenzoic acid, vitexin, isovitexin, and catechol. Both species showed high concentrations of phenols and total flavonoids and antioxidant activity. J. cordata showed the highest antioxidant capacity and the highest concentration of phenolic compounds. Overall, both Jatropha species are a natural source of antioxidant compounds with potential biotechnological uses.

Download Full-text

PROSPECTS FOR USING ASTRAGALUS ONOBRYCHIS AS A SOURCE OF NATURAL ANTIOXIDANTS

"Medical & pharmaceutical journal "Pulse" ◽

10.26787/nydha-2686-6838-2021-23-3-47-53 ◽

2021 ◽

pp. 47-53

Author(s):

Pozdnyakovа Т.А. ◽

Kuleshovа Е.S. ◽

Bubenchikov R.A.

Keyword(s):

Antioxidant Activity ◽

Free Radicals ◽

Chemical Interaction ◽

Raw Materials ◽

Natural Antioxidants ◽

The Body ◽

Plant Raw Materials ◽

Total Antioxidant ◽

Promising Source ◽

The Relationship

The predominance of synthetic and genetically modified foods in the diet of a modern person often leads to disruption of natural metabolic processes and the accumulation of toxic free radicals in the body, which provokes the development of various diseases. Natural antioxidants from medicinal plants can help maintain health and stop the processes of peroxidation. In our opinion, Аstragalus onobrychis can become a promising source of natural antioxidants. The aim of this study was to establish the antioxidant activity in extracts from the herb Astragalus onobrychis. In order to obtain objective and reliable results during our research, we used two methods: the method of titrimetry and spectrophotometry of free radicals. The titrimetric method is based on the chemical interaction between an oxidizing agent (potassium permanganate) and natural antioxidants present in extracts from plant raw materials, since they exhibit the properties of reducing agents. The spectrophotometric method makes it possible to assess the value of antioxidant activity and the duration of the reaction by assessing the interaction of 2,2-diphenyl-1-picrylhydrazil with plant antioxidants. To establish the relationship between the antioxidant activity and the presence of phenolic compounds in the plant, the content of flavonoids and hydroxycinnamic acids in the herb Astragalus onobrychis was determined. As a result of the studies, the total antioxidant activity of extracts from the herb Astragalus onobrychis was established and it was revealed that its value depends on the type of extractant used, since this factor is associated with the content of various groups of natural antioxidants in the extraction. The data obtained indicate the prospects of using the herb Astragalus onobrychis as a source of natural antioxidants.

Download Full-text

Quantitative estimation of total phenols, total flavonoids, and antioxidant activity of methanolic extracts of Ephedra gerardiana Wall. ex Stapf across different habitats of cold desert biosphere reserve, trans Himalaya, India

Medicinal Plants - International Journal of Phytomedicines and Related Industries ◽

10.5958/0975-6892.2020.00072.6 ◽

2020 ◽

Vol 12 (4) ◽

pp. 598-608

Author(s):

Deepti Negi ◽

Sher Singh Samant

Keyword(s):

Antioxidant Activity ◽

Quantitative Estimation ◽

Biosphere Reserve ◽

Total Flavonoids ◽

Total Phenols ◽

Cold Desert ◽

Methanolic Extracts

Download Full-text

Phenolic composition and antioxidant potential of different organs of Kazakh Crataegus almaatensis Pojark: A comparison with the European Crataegus oxyacantha L. flowers

Open Chemistry ◽

10.1515/chem-2018-0048 ◽

2018 ◽

Vol 16 (1) ◽

pp. 415-426 ◽

Cited By ~ 5

Author(s):

Elmira Bekbolatova ◽

Wirginia Kukula-Koch ◽

Tomasz Baj ◽

Natalia Stasiak ◽

Galiya Ibadullayeva ◽

...

Keyword(s):

Principal Component ◽

Natural Antioxidants ◽

Correlation Factor ◽

Total Phenolic ◽

Endemic Plant ◽

Leaf Extracts ◽

Polyphenolic Composition ◽

Antioxidant Potency ◽

Dpph Scavenging ◽

Promising Source

AbstractThe aim of this study was to investigate polyphenolic composition of different parts (leaves, flowers and fruits) of Crataegus almaatensis Pojark, an endemic plant of Kazakhstan, and compare it to a well known European Crataegus oxyacantha L. flowers. A Qual-Quant analysis was performed based on HR-MS measurements on 22 secondary metabolites: flavonoids and phenolic acids. Another goal was to evaluate the antioxidant potency of hawthorn extracts which was expressed in the total phenolic content and DPPH scavenging potency tests. Leaf extracts from C. almaatensis were found to be the most rich in metabolites and the most active in antiradical tests (IC50 value of 48 μg/ml and TPC of 218 mg/g). The weakest potential was determined for the fruit extract of this species. According to the performed principal component analysis (PCA), the fruit extracts were not correlated with other organs of the plant, and the metabolites responsible for the extracts’ differentiation were cyanidin 3-glucoside and quetcetin 3-galactoside. Based on a high correlation factor, the flowers of the Kazakh species was found to be as rich in polyphenols as the European hawthorn. The results of this study indicate that C. almaatensis is a promising source of natural antioxidants.

Download Full-text