scholarly journals UJI AKTIVITAS KOMBINASI EKSTRAK KERING LIDAH BUAYA (Aloe vera. (L) brum. f.) DAN EKSTRAK KENTAL DAUN SIRIH MERAH (Piper crocatum ruiz & pav) UNTUK ANTIBAKTERI PENYEBAB JERAWAT

2018 ◽  
Vol 15 (01) ◽  
pp. 12
Author(s):  
Suhaimi Suhaimi ◽  
Teti Indrawati ◽  
Shirly Kumala
Keyword(s):  
Staphylococcus Aureus ◽  
Leaf Extract ◽  
Propionibacterium Acnes ◽  
Aloe Vera ◽  
Inhibitory Effect ◽  
Dry Extract ◽  
Propionibacterium Acne ◽  
Betel Leaf ◽  
And Staphylococcus Aureus

INTISARI   Ekstrak kering lidah buaya dan ekstrak kental daun sirih merah mengandung senyawa alkaloid, flavonoid, tanin, saponin dan fenol yang mempunyai aktivitas sebagai antibakteri penyebab jerawat. Penelitian ini bertujuanUntuk menentukan konsentrasi ekstrak kering lidah buaya  (Aloe vera (L) brum f), untuk menentukan ekstrak kental daun sirih merah (Piper crotacum ruiz dan pav)  mempunyai daya hambat ter terhadap bakteri Propionibacterium acnes dan Staphylococcus aureus penyebab jerawat dan untuk menentukan konsentrasi kombinasi ekstrak kering lidah buaya  (Aloe vera (L) brum f)  dan ekstrak kental daun sirih merah  (Piper crotacum ruiz dan pav)mempunyai daya hambat terterhadap bakteri  Propionibacterium acnes  dan  Staphylococcus aureus  penyebab jerawat.Tahap-tahap penelitian yaitu determinasi tanaman lidah buaya dan daun sirih merah, pembuatan ekstrak, pemeriksaan ekstrak meliputi organoleptik, pH, uji skrining fitokimia serta pengujian aktivitas masing-masing ekstrak kering lidah buaya dengan konsentrasi 10%, 15%, 20%, 25%, 30% dan 35%, ekstrak kental daun sirih merah dengan konsentrasi 25%, 12,5%, 7,16%, 3,13%, 1,78% dan 0,78% serta kombinasi ekstrak kering lidah buaya dan ekstrak kental daun sirih merah dengan konsentrasi 50%, 25%, 12,5%, 7,16%, 3,13%,  dan 1,56 terhadap bakteri Propionibacterium acnes dan Staphylococcus aureus. Kombinasi ekstrak kering  lidah buaya  dan ekstrak kental daun sirih merah   memiliki aktivitas antibakteri terhadap bakteri  Staphylococcus aureus  pada konsentrasi 1,56 % dan Propionibacterium acne   pada konsentrasi  3,13  Kata kunci : Lidah buaya, daun sirih merah, Propionibacterium acnes, Staphylococcus aureus.  ABSTRACT  Dried aloe vera extract and thick red betel leaf extract contains alkaloid compounds, flavonoids, tannins, saponins and phenols that have activity as an antibacterial cause of acne. The aim of this study was to determine the concentration of Aloe vera (L) brum f) extract, to determine the thick red betel leaf extract (Piper crotacum ruiz and pav) to have the inhibitory effect against the bacteria Propionibacterium acnes and Staphylococcus aureus causes acne and  to determine the concentration of Aloe vera (L) brum f) and red thick betel leaf extract (Piper crotacum ruiz and pav) have inhibitory effect on bacteria Propionibacterium acnes and Staphylococcus aureus cause acne. The research stages are the determination of aloe vera and red betel leaf, extract preparation, extract examination including organoleptic, pH, phytochemical screening test and activity test of dry aloe vera extract with concentration 10%, 15%, 20%, 25% , 30% and 35%, thick red betel leaf extract with concentration of 25%, 12.5%, 7.16%, 3.13%, 1.78% and 0.78%, and combination of aloe vera extract and viscous extract red betel leaves with concentrations of 50%, 25%, 12.5%, 7.16%, 3.13%, and 1.56 against the bacteria Propionibacterium acnes and Staphylococcus aureus. The combination of dry extract of aloe vera and thick red betel leaf extract have antibacterial activity against Staphylococcus aureus bacteria at concentration 1.56% and Propionibacterium acne at concentration 3,13 %. Keywords: aloe vera, red betel leaf extract, Propionibacterium acnes, Staphylococcus aureus

scholarly journals Perbandingan Daya Hambat Larutan Antiseptik Povidone iodine dengan Ekstrak Daun Sirih terhadap Candida albicans secara In Vitro

2015 ◽  
Vol 4 (3) ◽  
Author(s):  
Septriana Putri ◽  
Aziz Djamal ◽  
Rahmatini Rahmatini ◽  
Cimi Ilmiawati
Keyword(s):  
Candida Albicans ◽  
Leaf Extract ◽  
Vaginal Discharge ◽  
Povidone Iodine ◽  
Inhibitory Effect ◽  
Iodine Solution ◽  
Betel Leaf ◽  
Post Hoc ◽  
Povidone Iodine Solution

Abstrak Candida albicansb (C. albicans) adalah salah satu mikroorganisme penyebab masalah kesehatan reproduksi wanita, yaitu keputihan (fluor albus). Penggunaan larutan povidone iodine dan bahan alam seperti ekstrak daun sirih menjadi pilihan masyarakat sebagai pembersih alat kewanitaan. Tujuan penelitian ini adalah untuk membandingkandaya hambat larutan antiseptik povidone iodine dan ekstrak daun sirih terhadap jamur C. albicans secara in vitro. Penelitian dilakukan terhadap lima isolat jamur C. albicans dengan larutan kontrol akuades.Perlakuan terdiri dari povidone iodine, ekstrak daun sirih dengan konsentrasi 5%, 10%, dan 20%.Hasil penelitian menunjukkan bahwa povidone iodine memiliki daya hambat terhadap C. albicans. Ekstrak daun sirih dengan konsentrasi 5% dan 10% tidak memiliki daya hambat terhadap C. albicans, namun ekstrak daun sirih konsentrasi 20% memiliki daya hambat terhadap C. albicans. Analisis statistik dengan uji ANOVA yang dilanjutkan dengan uji Post-hoc menunjukkan perbedaan bermakna antara daya hambat larutan povidone iodine dan ekstrak daun sirih 20% terhadap kontrol(p < 0.05).Larutan povidone iodine memiliki daya hambat dua kali lebih besar terhadap pertumbuhan C. albicans dibandingkan ekstrak daun sirih 20%. Dari penelitian ini dapat disimpulkan bahwa larutan povidone iodine dan ekstrak daun sirih 20% dapat menghambat pertumbuhan jamur C. albicans secara in vitro. Kata kunci: povidone iodine, ekstrak daun sirih, Candida albicansAbstract Candida albicans (C. albicans) is one of the frequent causes of  reproductive health problems in women, namely vaginal discharge (fluor albus). The antiseptic solution, povidone iodine, is still an option to overcome vaginal discharge. The use of natural materials such as betel (Piper betle L.) leaves extract also become a popular choice as adouche for women. The objective of this study was to compare the inhibitory activity of povidone iodine solution and betel leaf extract against the growth of C. albicans in vitro. We used five different isolates of C. albicans with distilled water as control. Each isolate was treated with povidone iodine solution, betel leaf extract at concentration of 5%, 10%,and 20%. The results showed that povidone iodine had inhibitory effect on C. albicans. Betel leaf extract at concentration of 5% and 10% did not have inhibitory effectwhile betel leaf extract at concentration of 20% hadinhibitory effect on C. albicans. Analysis by ANOVA followed by Post-hoc tests showed a significant difference in inhibitory activity of povidone iodine and betel leaf extract at 20% concentration compared to control (p < 0,05). Povidone iodine solution showed twice as much as inhibitory effect on C. albicans compared to betel leaf extract (20% concentration). It is concluded that povidone iodine solution and betel leaf extract at 20% concentration can inhibit the growth of C. albicans in vitro.Keywords: povidone iodine, betel leaf extract, Candida albicans

scholarly journals Efficacy of Telavancin against Penicillin-Resistant Pneumococci and Staphylococcus aureus in a Rabbit Meningitis Model and Determination of Kinetic Parameters

2006 ◽  
Vol 50 (2) ◽  
pp. 770-773 ◽  
Author(s):  
Armin Stucki ◽  
Peter Gerber ◽  
Fernando Acosta ◽  
Marianne Cottagnoud ◽  
Philippe Cottagnoud
Keyword(s):  
Staphylococcus Aureus ◽  
Body Weight ◽  
Kinetic Parameters ◽  
Intravenous Injections ◽  
Staphylococcal Strain ◽  
Pneumococcal Strain ◽  
And Staphylococcus Aureus

ABSTRACT The penetration of telavancin was 2% into inflamed meninges and ca. 1‰ into noninflamed meninges after two intravenous injections (30 mg/kg of body weight). In experimental meningitis, telavancin was significantly superior to vancomycin combined with ceftriaxone against a penicillin-resistant pneumococcal strain. Against a methicillin-sensitive staphylococcal strain, telavancin was slightly but not significantly superior to vancomycin.

scholarly journals Hop Extract Acts as an Antioxidant with Antimicrobial Effects against Propionibacterium Acnes and Staphylococcus Aureus

Molecules ◽  
2019 ◽  
Vol 24 (2) ◽  
pp. 223 ◽  
Author(s):  
Natalja Weber ◽  
Klaus Biehler ◽  
Kay Schwabe ◽  
Birgit Haarhaus ◽  
Karl-W. Quirin ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Propionibacterium Acnes ◽  
Inhibition Zone ◽  
Positive Control ◽  
Antioxidative Effect ◽  
Minimal Inhibitory Concentrations ◽  
Anti Inflammatory ◽  
And Staphylococcus Aureus ◽  
And Oxidative Stress ◽  
Antibacterial Potential

Acne is associated with hyperkeratosis, elevated levels of skin sebum and growth of Propionibacterium acnes (P. acnes) and Staphylococcus aureus (S. aureus). Furthermore, P. acnes promotes inflammation by inducing IL-6 production and oxidative stress. The aim of this study was to assess the antioxidant, anti-inflammatory and antibacterial potential of a hop-CO2-extract with 50% humulone and lupulone. The susceptibility of P. acnes and S. aureus to the hop extract was tested by using the broth microdilution technique. The minimal inhibitory concentrations (MIC) for P. acnes and S. aureus were 3.1 and 9.4 µg/mL, respectively. In addition, the hop extract showed an antioxidative effect with a half maximal inhibitory concentration (IC50) of 29.43 µg/mL as well as additional anti-inflammatory effects by reducing the IL-6 expression (IC50: 0.8 µg/mL). In addition, a gel formulation with 0.3% hop extract (w/w) had antibacterial activity against P. acnes and S. aureus (inhibition zone value: 5.5 mm and 3 mm, respectively) which was significantly superior to the placebo gel. The positive control (a gel with the antibiotic clindamycin) showed an inhibition zone of 9 mm. Due to its antioxidant, anti-inflammatory and antibacterial effects hop extract might be a treatment option for acne-prone skin.

scholarly journals Uji Aktivitas Antimikroba Ekstrak Daun Mahkota Dewa (Phaleria macrocarpa) dengan Metode Difusi Cakram

2020 ◽  
Vol 6 (1) ◽  
pp. 8
Author(s):  
M. Fiqriansyah Wahab ◽  
Yustika Indahsari ◽  
Nurdiana Nurdiana ◽  
Andi Maghfira Manggabarani ◽  
Putri Bella Aulia Nur
Keyword(s):  
Staphylococcus Aureus ◽  
Medicinal Plant ◽  
Bacterial Growth ◽  
Leaf Extract ◽  
Inhibitory Zone ◽  
E Coli ◽  
Phaleria Macrocarpa ◽  
And Staphylococcus Aureus

Indonesia has abundant biological rich potential so it needs to be researched and utilized especially for various plants that have potential as medicine. One of the plants that can be used as a medicinal plant and has many benefits is the Phaleria macrocarpa. This study aimed to determine the effectiveness of P. macrocarpa leaf extract to inhibit the growth of Escherecia coli and Staphylococcus aureus bacteria. The results showed that the leaf extract of P. macrocarpa containing tannin compounds has the ability to inhibit bacterial growth. The extract of the crown of the deity of the god is more effective in inhibiting the growth of S. aureus bacteria compared to E. coli bacteria which is shown with a larger inhibitory zone. It means that the extract of the P. macrocarpa can be used as a medicinal ingredient for diseases caused by S. aureus bacteria

scholarly journals Aktivitas Antibakteri Ekstrak Daun Jarak Pagar (Jatropha Curcas L) Terhadap Staphylococcus Epidermidis Dan Staphylococcus Aureus Secara In Vitro

2018 ◽  
Vol 7 (1) ◽  
Author(s):  
Susilo Yulianto ◽  
Sunarmi Sunarmi
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Jatropha Curcas ◽  
Staphylococcus Epidermidis ◽  
Leaf Extract ◽  
Inhibitory Zone ◽  
Zone Diameter ◽  
Jatropha Curcas L ◽  
Inhibitory Power ◽  
And Staphylococcus Aureus

Abstract: Jatropha Leaves, Staphylococcus Epidermidis, Staphylococcus Aureus. Jatropha curcas L leaves contain tannins, saponins and flavonoids. According to some studies, tannins, saponins and flavonoids have antibacterial power. Based on these indications, it is important to do research on antibacterial potency of jatropha leaf extract (Jatropha curcas L) against Staphylococcus epidermidis and Staphylococcus aureus bacteria. The aim of this research is to know Antibacterial Activity of Jatropha curcas L Extract on Staphylococcus epidermidis and Staphylococcus aureus". This research. An experimental study by testing the antibacterial activity of leaf extract and jatropha result of maceration, infundation and reflux as measured by Minimal Inhibitory Concentration (KHM) on growth of Staphylococcus epidermidis and Staphylococcus aureus bacteria. From the results of the research, it was found that the extract of the reflux was brownish green and the characteristic of jatropha leaves. The amount of extract produced was 17.03 g, so the yield was 17.03%. Jatropha leaf extract of maceration has no inhibitory power to S. epidermidis in 4 series concentrations starting from 3.125 ppm to 25.000 ppm. Inhibitory zones begin to appear at concentrations of 50,000 ppm and increase at concentrations of 100,000 ppm. The drag zone diameter in the Jatropha curcas extract of the reflux showed the inhibitory power in all series of concentrations. The smallest inhibitory zone was shown at a concentration of 3,125 ppm (9.33 mm) and increased with increasing concentrations. The diameter of the inhibit zone was greatest at 100,000 ppm concentrations of 22.67 mm. Description of inhibitory zone diameter of methanol leaf extract of jatropha result of maceration and reflux to S. epidermidis. The conclusion of this research is extract of reflux result giving bigger resistance than maceration extract to S. epidermidis and S. aureus. Methanol extract of jatropha from the maceration and reflux have strong antibacterial activity against S. epidermidis and S. aureus but still under Klindamycin.

scholarly journals Antibacterial Activity of Extract and Fraction From Shitake Mushroom (Lentinula edodes) Against Acne Bacteria

2018 ◽  
Vol 6 (1) ◽  
pp. 36
Author(s):  
Ika Kurnia Sukmawati ◽  
Ari Yuniarto ◽  
Widhya Alighita ◽  
Ade Zam-zam J
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Staphylococcus Epidermidis ◽  
Propionibacterium Acnes ◽  
Ethyl Acetate Fraction ◽  
Hexane Fraction ◽  
Lentinus Edodes ◽  
Shiitake Mushrooms ◽  
And Staphylococcus Aureus

Acne is an inflammatory disease that occurs in the skin triggered by the bacteria acne treatment can be done by using natural materials that shiitake mushrooms (Lentinus edodes). This aims of this study were to determining the antibacterial activity of extracts and fractions shiitake mushrooms with broth microdilution method, determining the value of equality shiitake mushrooms with antibacterial comparison and determining the morphological changes of bacteria after exposure to the test sample with a Scanning Electron Microscope (SEM). Tests conducted antibacterial activity against Propionibacterium acnes, Staphylococcus epidermidis and Staphylococcus aureus at concentrations used is 1 ppm, 2 ppm, 4 ppm, 8 ppm, 16 ppm, 32 ppm, 64 ppm, 128 ppm, 256 ppm, 512 ppm. Best MIC value obtained in fraction of ethyl acetate and n-hexane fraction of the bacterium Propionibacterium acnes, Staphylococcus epidermidis and Staphylococcus aureus at a concentration of 256 ppm. KBM value of the n-hexane fraction against Staphylococus aureus bacteria at concentrations of 512 ppm and ethyl acetate fraction against bacteria Staphylococus aureus and epidermidis at a concentration of 512 ppm. Value equality is obtained 1 mg of ethyl acetate fraction of shiitake mushrooms equivalent to 5.346 x 10-2 mg of tetracycline. SEM test results showed the presence of antibacterial activity which is indicated by a change in cell morphology, their lumps and their cell wall frown on Propionibacterium acnes were exposed to ethyl acetate fraction.Key words: Acne, Lentinus edodes, antibacterials, microdilution, SEM

Antimicrobial and preservative effects of the combinations of nisin, tea polyphenols, rosemary extract and chitosan on pasteurized chicken sausage

2020 ◽  
Author(s):  
Kekui Sun ◽  
Shanglong Wang ◽  
Qingfeng Ge ◽  
Guoyuan Xiong ◽  
Xi Zhou ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Low Temperature ◽  
Shelf Life ◽  
Inhibitory Effect ◽  
Tea Polyphenols ◽  
Rosemary Extract ◽  
Clear Zone ◽  
Antioxidant Effects ◽  
And Staphylococcus Aureus

The study evaluated the antimicrobial and antioxidant effects of the combinations of nisin (NS), tea polyphenols (TP), rosemary extract (RE) and chitosan (CS) on low-temperature chicken sausage. An orthogonal test revealed that the most effective antimicrobial compositions were equal-quantity mixtures of 0.05% NS + 0.05% TP + 0.03% RE + 0.55% CS . The mixture also produced strong antimicrobial and antioxidant effects in low-temperature chicken sausage related to extend the shelf life to more than 30 days at 4°C. The study also investigated the inhibitory zone of NS, TP, RE and CS against Pseudomonas aeruginosa , lactic acid bacteria (LAB) and Staphylococcus aureus which were the dominant spoilage bacteria in low-temperature chicken sausage. NS had the greatest inhibitory effect on LAB and Staphylococcus aureus , exhibiting clear zone diameters of 19.7 mm and 17.8 mm respectively. TP had the largest inhibitory effect on Pseudomonas aeruginosa , exhibiting a clear zone diameter of 18.2 mm. These results indicated that the combination of NS, TP, RE and CS could be used as natural preservative s to efficiently inhibit the growth of spoilage microorganisms in low-temperature chicken sausage so as to improve its safety and shelf life.

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