scholarly journals Keefektifan Lecanicillium lecanii Mengendalikan Crocidolomia pavonana Pada Skala Laboratorium

2022 ◽  
Vol 5 (1) ◽  
pp. 15-19
Author(s):  
Helmi Natul Wildan ◽  
Efrin Firmansyah ◽  
Siti Nurhidayah
Keyword(s):  
Lecanicillium Lecanii

ABSTRAK Cendawan L. lecanii adalah salah satu agens hayati yang sangat potensial untuk dimanfaatkan dalam pengendalian beberapa hama dan penyakit tanaman. Percobaan ini dilaksanakan di Laboratorium Satuan Pelayanan Balai Perlindungan Tanaman Pangan dan Hortikultura Wilayah V Tasikmalaya Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) faktor tunggal dengan 6 perlakuan dan 5 ulangan, yaitu Konsentrasi L. lecanii : P0 (Kontrol), P1 (5 g.L-1), P2 (10 g.L-1), P3 (15 g.L-1), P4 (20 g.L-1), P5 (25 g.L-1). Variabel yang yang diamati yaitu mortalitas, konsumsi pakan larva c. pavonana, perilaku larva yang terinfeksi, lama waktu kematian larva. Perlakuan yang menunjukan hasil optimal ada pada perlakuan P5 (Konsentrasi L. lecanii 25 g.L-1) dengan nilai mortalitas 82%, konsumsi pakan 1.23 g.perlakuan-1, lama waktu kematian 1.8 HSA dan perilaku larva yang terinfeksi yaitu C. pavonana mengalami perubahan warna, yang awalnya berwarna kuning berubah menjadi warna hitam. Tubuh larva menjadi keras, karena semua jaringan dan cairan dalam tubuh larva habis oleh cendawan L. lecanii, lalu secara perlahan diselimuti oleh miselium.  

scholarly journals Sarocladium and Lecanicillium Associated with Maize Seeds and Their Potential to Form Selected Secondary Metabolites

Biomolecules ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 98
Author(s):  
Lidia Błaszczyk ◽  
Agnieszka Waśkiewicz ◽  
Karolina Gromadzka ◽  
Katarzyna Mikołajczak ◽  
Jerzy Chełkowski
Keyword(s):  
Large Subunit ◽  
Solid Substrate ◽  
Rrna Gene ◽  
Decenoic Acid ◽  
Bioactive Substances ◽  
Lecanicillium Lecanii ◽  
Maize Seeds ◽  
Internally Transcribed Spacer ◽  
First Time

The occurrence and diversity of Lecanicillium and Sarocladium in maize seeds and their role in this cereal are poorly understood. Therefore, the present study aimed to investigate Sarocladium and Lecanicillium communities found in endosphere of maize seeds collected from fields in Poland and their potential to form selected bioactive substances. The sequencing of the internally transcribed spacer regions 1 (ITS 1) and 2 (ITS2) and the large-subunit (LSU, 28S) of the rRNA gene cluster resulted in the identification of 17 Sarocladium zeae strains, three Sarocladium strictum and five Lecanicillium lecanii isolates. The assay on solid substrate showed that S. zeae and S. strictum can synthesize bassianolide, vertilecanin A, vertilecanin A methyl ester, 2-decenedioic acid and 10-hydroxy-8-decenoic acid. This is also the first study revealing the ability of these two species to produce beauvericin and enniatin B1, respectively. Moreover, for the first time in the present investigation, pyrrocidine A and/or B have been annotated as metabolites of S. strictum and L. lecanii. The production of toxic, insecticidal and antibacterial compounds in cultures of S. strictum, S. zeae and L. lecanii suggests the requirement to revise the approach to study the biological role of fungi inhabiting maize seeds.

scholarly journals Lecanicillium lecanii (Zimmermann) Zare & Gams, as an efficient biocontrol agent of tea thrips, Scirtothrips bispinosus Bagnall (Thysanoptera: Thripidae)

2021 ◽  
Vol 31 (1) ◽  
Author(s):  
Mariappan Sankara Rama Subramaniam ◽  
Azariah Babu ◽  
Bhabesh Deka
Keyword(s):  
Tamil Nadu ◽  
South India ◽  
Biocontrol Agent ◽  
Uv Light ◽  
Biological Control Agents ◽  
Optimum Conditions ◽  
Lecanicillium Lecanii ◽  
Wettable Powder ◽  
Label Claim ◽  
Yeast Powder

Abstract Background Continuous and non-judicial application of synthetic insecticides to control the tea thrips, Scirtothrips bispinosus (Bagnall), one of the major tea pests in South India has led to certain undesirable issues in the ecosystem besides the presence of the pesticide residues in manufactured tea. Biological control agents are of immense importance in tea cultivation. The present study was designed to isolate Lecanicillium lecanii (Zimmermann) Zare & Gama from the field-collected cadavers of the insects/mites infected by fungi of tea growing areas of Anamallais (Tamil Nadu, South India), and to evaluate their field bio-efficacy against the tea thrips. Results Lecanicillium lecanii isolated from the tea ecosystem had been formulated into a wettable powder (WP) formulation and evaluated against tea thrips under both laboratory and field conditions. Among the several media evaluated, the PDAY (Potato Dextrose Agar + 1% Yeast powder) was found to be the best suitable medium for the growth and germination of spores. Optimum conditions for the growth of L. lecanii were found in PDAY medium at the pH 6-7, temperature 25-30°C and 90-95% RH. Exposure to UV light for more than 30 min significantly inhibited the growth of the fungus. Lecanicillium lecanii at (1 × 107 spore/ha) was found significantly effective against thrips. Fungal development index (FDI) of L. lecanii + jaggery significantly differed than other treatments. Lecanicillium lecanii at 1500g (1×107 conidia/ml) mixed in 400 l of water was effective against the tea thrips. Addition of equal amount of jaggery with L. lecanii wettable powder in the tank mixture could increase the efficacy of the mycopesticide against tea thrips. Conclusion The powder formulation of L. lecanii was found safer to natural enemies present in the tea ecosystem. After fulfilling the requirements for its registration and label claim on tea, this strain of L. lecanii could be commercialized for the benefit of the tea industry for the management of tea thrips in an eco-friendly manner.

scholarly journals Entomopathogenic fungus, Lecanicillium lecanii R. Z are & W. Gams anchored into MCM-41: A new and effective bio-insecticide against Brevicoryne brassicae (Linnaeus, 1758) (Hom: Aphididae) to protect cabbages

2019 ◽  
Vol 114 (1) ◽  
pp. 97
Author(s):  
Asmar SOLEYMANZADE ◽  
Fereshteh KHORRAMI ◽  
Hana BATMANI ◽  
Khadijeh OJAGHI AGHBASH ◽  
Youbert GHOSTA
Keyword(s):  
Entomopathogenic Fungus ◽  
Cost Effective ◽  
Brevicoryne Brassicae ◽  
Vegetable Crops ◽  
Cabbage Aphid ◽  
Lecanicillium Lecanii ◽  
Insecticidal Efficacy ◽  
Potential Strategy ◽  
Nano Formulation ◽  
Mcm 41

<p class="Default"><em>Brevicoryne brassicae</em> is a significant pest of cultivated cabbages and vegetable crops in the world. The present study was carried out to examine a potential strategy to enhance the insecticidal activity of <em>Lecanicillium lecanii</em> for cost-effective management of <em>B. brassicae</em>. The insecticidal efficacy of pure entomopathogenic fungus (PEF) and MCM-41 (Mobil Composition of Matter)<em> L. lecanii</em> were assessed against the cabbage aphid under laboratory and greenhouse conditions. The fungus was supported on MCM-41 and was completely characterized by Scanning Electron Microscope (SEM), thermogravimetric analysis (TGA) and Fourier transform infrared (FT-IR) techniques. LC50 values of PEF and MCM-41@fungus were 1.9×106 and 2.5×104 and 2.0×107 and 2.0×105 conidia/ml on adults of <em>B. brassicae</em> under laboratory and greenhouse conditions, respectively. Bioassays demonstrated that MCM-41@fungus significantly decreased LC50 values of entomopathogenic fungus and it was more toxic than <em>L. lecanii</em> at adult stage of the pest. The results showed that pure <em>L. lecanii</em> and its nano-formulation could play key roles as bio-pesticides in <em>B. brassicae</em> management programs.</p>

scholarly journals DETERMINAÇÃO DA ÉPOCA DE LIBERAÇÃO DE LYSIPHLEBUS TESTACEIPES (CRESSON,1880) (HYMENOPTERA: APHIDIIDAE) APÓS APLICAÇÃO DE DIFERENTES FUNGOS ENTOMOPATOGÊNICOS PARA CONTROLE INTEGRADO DE SCHIZAPHIS GRAMINUM (ROND., 1852) (HEMIPTERA: APHIDIDAE)

2009 ◽  
Vol 76 (2) ◽  
pp. 251-257
Author(s):  
R.S. Cavalcanti ◽  
A. Moino Junior ◽  
E.S. Loureiro ◽  
L.A. Mendonça ◽  
A. Arnosti
Keyword(s):  
Metarhizium Anisopliae ◽  
Schizaphis Graminum ◽  
Paecilomyces Fumosoroseus ◽  
Lysiphlebus Testaceipes ◽  
Lecanicillium Lecanii

RESUMO As interações entre patógenos e parasitóides devem ser avaliadas para um possível controle das pragas, sendo observada a interrupção ou não do desenvolvimento larval quando um hospedeiro parasitado é infectado ou quando um hospedeiro infectado é oferecido ao parasitóide. O objetivo deste trabalho foi avaliar o melhor tempo de liberação do parasitóide Lysiphlebus testaceipes e a melhor concentração dos fungos Beauveriabassiana, Metarhizium anisopliae, Paecilomyces fumosoroseus e Lecanicillium lecanii no controle do pulgão Schizaphis graminum. Os parasitóides foram liberados às 0, 24, 48 e 72 horas após aplicação de duas concentrações (104 e 108 conídios/mL) dos fungos B. bassiana, M. anisopliae, P. fumosoroseus e L. lecanii. Na testemunha foi pulverizada água destilada esterilizada. O fungo entomopatogênico L. lecanii é altamente prejudicial ao desenvolvimento do parasitóide na maior concentração. Para a utilização conjunta dos fungos B. bassiana, M. anisopliae e P. fumosoroseus com L. testaceipes no controle de S. graminum, a melhor época de liberação do parasitóide é logo após a sua aplicação (0h).

Lecanicillium lecanii. [Descriptions of Fungi and Bacteria].

2003 ◽  
Author(s):  
R. Zare
Keyword(s):  
New Zealand ◽  
Great Britain ◽  
South America ◽  
Central America ◽  
Geographical Distribution ◽  
The Body ◽  
Green Coffee ◽  
Lecanicillium Lecanii ◽  
Coccus Viridis ◽  
Insect Family

Abstract A description is provided for Lecanicillium lecanii. Information is included on the disease caused by the organism, its transmission, geographical distribution, and hosts. DISEASE: Insect-pathogenic; the fungus penetrates the body of its hosts, sporulates and eventually kills them. HOSTS: Members of the homopteran insect family Coccidae, particularly the green coffee scale, Coccus viridis. Other hosts: Ceroplastes (Lo & CHAPMAN, 1998), Parthenolecanium (SAMŠINAKOVÀ & KALALOVÀ, 1975), Philephedra (PENA & MCMILLAN, 1986), Planococcus (GONZÀLEZ et al., 1995), Saissetia (MENDEL et al., 1984) and a black Aleyrodes (PETCH, 1925). Cephalosporium coccorum was recorded on Chionaspis and Lepidosaphes in England (PETCH, 1925). GEOGRAPHICAL DISTRIBUTION: Mainly tropical. NORTH AMERICA: USA. CENTRAL AMERICA: Cuba, Dominican Republic, Jamaica. SOUTH AMERICA: Ecuador (Galápagos Is), Peru. ASIA: China, India, Indonesia, Sri Lanka. AUSTRALASIA: New Zealand. EUROPE: Great Britain, Turkey. TRANSMISSION: Soil- and air-borne.

Biosafety of pesticides and entomopathogens to the anthocorid predator Blaptostethus pallescens Poppius (Heteroptera: Anthocoridae)

ENTOMON ◽  
2020 ◽  
Vol 45 (4) ◽  
pp. 253-258
Author(s):  
A. Nasreen ◽  
S. Sridharan ◽  
M. Muthukumar ◽  
K. Ashok
Keyword(s):  
Beauveria Bassiana ◽  
Laboratory Experiments ◽  
Egg Laying ◽  
Lecanicillium Lecanii

Laboratory experiments conducted to study the biosafety of insecticides, acaricides and entomopathogens viz. imidacloprid, chlorpyriphos, fenazaquin, spiromesifen, azadirachtin, Beauveria bassiana and Lecanicillium lecanii in different concentrations to Blaptostethus pallescens, revealed that imidacloprid and chlorpyriphos were highly toxic with maximum mortality. The acaricides fenazaquin and spiromesifen were less toxic which recorded minimum mortality of predator and more egg laying and hatching. Azadirachtin showed higher oviposition and was on par with spiromesifen. Azadirachtin and acaricides are compatible with B. pallescens than entomopathogens.

scholarly journals Amino Acids in Entomopathogenic Fungi Cultured In Vitro

Agronomy ◽  
2020 ◽  
Vol 10 (12) ◽  
pp. 1899
Author(s):  
Lech Wojciech Szajdak ◽  
Stanisław Bałazy ◽  
Teresa Meysner
Keyword(s):  
Amino Acids ◽  
Entomopathogenic Fungi ◽  
Pathogenic Fungi ◽  
Individual Species ◽  
Conidiobolus Coronatus ◽  
Fungal Strains ◽  
Isaria Farinosa ◽  
Lecanicillium Lecanii ◽  
Wide Range

The content of bounded amino acids in six entomopathogenic fungi was identified and determined. Analyzing the elements characterizing the pathogenicity of individual species of fungi based on infectivity criteria, ranges of infected hosts, and the ability to induce epizootics, these can be ranked in the following order: Isaria farinosa, Isaria tenuipes, Isaria fumosorose, Lecanicillium lecanii, Conidiobolus coronatus, Isaria coleopterorum. These fungi represent two types of Hyphomycetales-Paecilomyces Bainier and Verticillium Nees ex Fr. and one type of Entomophtorales-Conidiobolus Brefeld. Our study indicates that there are significant quantitative and qualitative differences of bounded amino acids in the entomopathogenic fungal strains contained in the mycelium between high and low pathogenicity strains. The richest composition of bounded amino acids has been shown in the mycelium of the Isaria farinosa strain, which is one of the most commonly presented pathogenic fungi in this group with a very wide range of infected hosts and is the most frequently recorded in nature as an important factor limiting the population of insects.

Novel transglycosylation activity of β-N-acetylglucosaminidase of Lecanicillium lecanii produced by submerged culture

2020 ◽  
Vol 145 ◽  
pp. 759-767 ◽  
Author(s):  
Jesús Rojas-Osnaya ◽  
Zaizy Rocha-Pino ◽  
Hugo Nájera ◽  
Humberto González-Márquez ◽  
Keiko Shirai
Keyword(s):  
Submerged Culture ◽  
Lecanicillium Lecanii ◽  
Transglycosylation Activity

scholarly journals Postharvest Treatment of Tribolium confusum Jacquelin du Val Adults with Commercial Biopesticides

Agriculture ◽  
2019 ◽  
Vol 9 (10) ◽  
pp. 226 ◽  
Author(s):  
Spiridon Mantzoukas ◽  
Ioannis Lagogiannis ◽  
Ioannis Pettas ◽  
Georgia Korbou ◽  
Alexandra Magita ◽  
...  
Keyword(s):  
Tribolium Confusum ◽  
Insecticidal Effect ◽  
Lecanicillium Lecanii ◽  
Linum Usitatissimum L ◽  
Anova Model ◽  
Kaplan Meier ◽  
Postharvest Treatment ◽  
Mortality Percentage ◽  
Confused Flour Beetle ◽  
Coleoptera Tenebrionidae

Within the context of the harmful side-effects of chemical pest control applications, the present study investigated the insecticidal effect of three commercial biopesticides, the fungal Metab (Beauveria bassiana, Metarhizium anisopliae) and Lecan (Lecanicillium lecanii), as well as raw zeolite, against Tribolium confusum Jacquelin du Val (Coleoptera: Tenebrionidae), also known as the confused flour beetle. To this end, we sprayed Tribolium confusum adults with suspensions of the said biopesticides, at three different dosages (250 ppm, 500 ppm, and 1000 ppm) on Avena sativa L. and Linum usitatissimum L. hull and no hull seeds. The data were analyzed in terms of three- and four-way ANOVA model, and the overall survival was determined while using the Kaplan–Meier method. The mortality of Tribolium confusum adults was recorded and analyzed in correlation with the following parameters: dose, product (seed), days, and treatment as factors. At the end of the experiment, all of the biopesticides were effectively pathogenic, but there was variation in their effectiveness in terms of the T. confusum mortality that they caused, depending on the product (seed). The type of seed can play a role in the pathogenicity or effectiveness of the biopesticides. Additionally, our results showed that the mortality percentage was dependent on the dose and treatment of the commercial biopesticides.

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