scholarly journals Aktivitas Enzim Amilase Isolat Bakteri Amilolitik dari Tepung Sagu Basah dan Lingkungan Tempat Penyediaannya Secara Tradisional di Jayapura

2018 ◽  
Vol 6 (2) ◽  
pp. 47-52
Author(s):  
Suprapto Surapto ◽  
Tri Gunaedi ◽  
Basa T. Rumahorbo
Keyword(s):  
Enzyme Activity ◽  
Agar Medium ◽  
Amylase Activity ◽  
Soluble Starch ◽  
Bacterial Isolates ◽  
Clear Zone ◽  
Plate Method ◽  
Crude Enzyme Extract ◽  
Nutrient Agar Medium ◽  
Bacillus Subtilis Atcc

The study about the activity of the enzyme amylase from amylolytic bacterial isolates from wet sagoo starch and  its traditional provision environment had been done in Jayapura. The purposes of this study were to determine the activity of amylase enzyme and to identify the bacteria isolated from wet sagoo starch and its processing environment in Jayapura district. The method used was an experimental laboratorium in which isolation of amylolytic bacteria was performed by using nutrient agar medium with 1% soluble starch on spreed pour plate method. The enzyme activity was detected with 0.2% iodine in 2% potassium iodide which were able to form a clear zone. The protein content of the crude enzyme extract was determined by the Bradford method using bovine serum albumin (BSA). Amylase enzyme activity was determined by the formula: DUN/ml = [(R0-R1)/R0] [dilution factor] DUN/ml (dextrinizing units per ml). The results showed that there were 15 isolates amylolytic bacteria. Four (4) bacterial isolates have amylolytic power of more than 30 mm. The amilase activity of amylolytic bacterial of all  isolates were quite high: which were 35 577, 18 903,  32 106 and 46 600 U/mg for SU4, SU13, SU23 and SU40 respectively. The identification of isolates indicated that the three isolates are members of the Bacillus cereus ATCC 14 579 types with a similarity value of 71.70% to 81.10%, and one isolate is Bacillus subtilis ATCC 6501 members with a similarity value of 94.30%. Keywords: Amylolytic bacteria, amylase activity, characterization, sago flour.

scholarly journals Screening of Extracellular Keratinase Producing Bacteria from Feather Processing Areas in Vellore, Tamil Nadu, India

2010 ◽  
Vol 2 (3) ◽  
pp. 559 ◽  
Author(s):  
H. Prasad V ◽  
G. Kumar ◽  
Kartik L ◽  
B. Rao KV
Keyword(s):  
Enzyme Activity ◽  
Tamil Nadu ◽  
Agar Medium ◽  
Agar Plate ◽  
Soil Samples ◽  
Bacillus Sp ◽  
Bacterial Isolates ◽  
Plate Method ◽  
Keratinolytic Activity ◽  
Plating Method

The aim of the current study was to isolate keratinolytic bacteria from the soil samples collected from different feather processing areas in Vellore, TN, India. The isolation was performed by serial dilution and plating method. Total eight bacteria were isolated from the collected soil samples. All isolates were screened for keratinolytic activity by Casein agar plate method, among eight bacterial isolates only one (H5) isolate showed the keratinolytic activity in Casein agar medium. H5 isolate (potential strain) was identified as Bacillus sp. by microscopic and biochemical experiments. The best enzyme activity was observed at pH 7 and temperature 30ºC.  Keywords: Keratinolytic; Spread plate method; Bacillus sp.; Bacteria. © 2010 JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights reserved. DOI: 10.3329/jsr.v2i3.4567              J. Sci. Res. 2 (3), 559-565 (2010) 

Isolasi dan Potensi Enzim Hidrolase Bakteri Simbion Padina sp. dari Pantai Lengkuas Belitung

2021 ◽  
Vol 23 (1) ◽  
pp. 11-17
Author(s):  
Siti Nur Jannah ◽  
Yumna Rahmadias Hanifa ◽  
Adi Budi Utomo ◽  
Ashar Kurnia Dian Prambodo ◽  
Arina Tri Lunggani
Keyword(s):  
Marine Organism ◽  
Marine Ecosystem ◽  
Gram Negative Bacteria ◽  
Scale Production ◽  
Market Demand ◽  
Bacterial Isolates ◽  
Clear Zone ◽  
Plate Method ◽  
Sea Use ◽  
Industrial Scale Production

Marine organism is one of the riches in the ocean of Indonesia. The benefits of sea use for new products produced are widely used and have high market demand. Enzymes that have marine interests have unique properties and have good benefits for industry. This study aims to isolate the bacteria that have symbionts with Padina sp and determine the potential of the enzyme hydrolase produced by these bacteria. Isolation is done by the spread plate method. Pure isolates obtained were then tested for the potential of the enzyme hydrolase on selective media. Clear zone measurements are performed to determine which bacterial isolates are good for enzyme production. The results obtained by 6 isolates of pure bacteria, all of which include Gram negative bacteria that form bacilli. All isolates had the ability to produce different Protease, Lipase, Amylase and Cellulase enzymes. The enzymes obtained from these symbiotic bacteria are expected to be used for industrial-scale production in Indonesia. In addition, the presence of this symbiont bacteria is able to reduce the level of exploitation of Padina sp and contribute to preserving the marine ecosystem.

scholarly journals The Effectivity of Bacteria Isolated From of Liquid Waste Palm Oil Plantation on Ganoderma Boninense

2019 ◽  
Vol 1 (1) ◽  
pp. 1-7
Author(s):  
Fitratul Aini
Keyword(s):  
Palm Oil ◽  
Oil Palm ◽  
Root Rot ◽  
Agar Medium ◽  
Pathogenic Fungus ◽  
Liquid Waste ◽  
Ganoderma Boninense ◽  
Bacterial Isolates ◽  
Basal Stem Rot ◽  
Nutrient Agar Medium

Ganoderma boninense is one of the main pathogenic fungus in oil palm plantations. Generally, these pathogen cause root rot (basal stem rot). Biological control that has been widely used reduce the infection is using bacteria. Liquid waste palm oil has potential to produce bacteria that is able to degrade Ganoderma boninense that causes root rot in oil palm. Liquid waste were obtained from Muaro Sabak Regency Jambi Province. Bacteri were isolated and cultivated in nutrient agar medium, characterized and identified for antagonistic test against G. boninense. Results showed that 16 bacterial isolates were identified, among of them are able to inhibit Ganoderma boninense.

scholarly journals Study of Microbiological Quality of Biscuits from Street Side Bakery Shops at Dehradun City, India

2020 ◽  
pp. 319-326
Author(s):  
Irtiqa Syed ◽  
Rajendra Prasad ◽  
Adeeba Naaz
Keyword(s):  
Agar Medium ◽  
Microbiological Quality ◽  
Nutrient Agar ◽  
Morphological Observation ◽  
Plate Count ◽  
Bacterial Isolates ◽  
Gram Positive ◽  
Gram Positive Bacteria ◽  
Nutrient Agar Medium

<p>In the present study, microbiological quality of biscuits from street side bakery shops at Dehardun city, India was conducted. A total 11 samples of bakery biscuits were collected randomly and analysed for their microbiologically quality by standard plate count method. Pure cultures of bacterial isolates were prepared by streaking on nutrient agar medium. Bacterial isolates were further studied for morphological characters, culture characters on nutrient agar medium and biochemical testes in laboratory. All samples studies were found contaminated by a variety of bacteria. The highest bacterial load was 2.2 ×105 cfu/g and lowest was 0.1×103 cfu/g, respectively. Morphological observation, culture characters and results of biochemical tests of bacterial isolates were compared with standard results of known bacteria. The bacterial isolated were identified as Alcaligenes faecalis, Bacillus cereus, Micrococcus luteus, Pseudomonas aeruginosa, Streptococcus lactis and Staphylococcus aureus, respectively. Among these, two isolates i.e., A. faecalis and P. aeruginosa were gram negative and other isolates were gram positive bacteria which suggested the poor hygienic conditions inside the bakery during preparation and storage of biscuits. Gram positive bacteria might be contaminated during storage and by bad handling. These food spoiling bacteria might be responsible for food-borne infection and diseases. Therefore, care should be taken while purchasing such products and should be purchased from certified bakery shop.</p>

scholarly journals The potential of amylase enzyme activity against bacteria isolated from several lakes in East Java, Indonesia

2020 ◽  
Vol 22 (1) ◽  
Author(s):  
INDAH KHOIRUN NISA ◽  
Prabaningtyas Sitoresmi ◽  
BETTY LUKIATI ◽  
RINA TRITURANI SAPTAWATI ◽  
ACHMAD RODIANSYAH
Keyword(s):  
Enzyme Activity ◽  
Molecular Identification ◽  
Bacterial Isolate ◽  
Amylase Activity ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Neighbor Joining ◽  
Bacterial Isolates ◽  
Bacillus Cereus Group ◽  
The 16S Rrna Gene

Abstract. Nisa IK, Prabaningtyas S, Lukiati B, Saptawati RT, Rodiansyah A. 2021. The potential of amylase enzyme activity against bacteria isolated from several lakes in East Java, Indonesia. Biodiversitas 21: 42-49. Indonesia is one country that has water resources having an abundance of microbial diversity, but not explored massively. This study aims to measure the amylase activity quantitatively from 53 amylolytic bacterial isolates from Ranu Pani, Ranu Regulo, Ranu Grati, and Ngebel Lake; also it identifies the isolate with the highest amylase enzyme activity. The amylase enzyme activity test calculates with DNS (Dinitrosalycylic acid) method, molecular identification of the highest bacterial isolate is based on the 16S rRNA gene. Its relationship is determined through the phylogenetic tree with the Neighbor-Joining (NJ) method. The results showed that the fifty-three bacterial isolates have amylase activity about 0.000-0.016 units/mL. The KN bacterial isolate from Ranu Ngebel was the highest amylase activity, producing enzyme around 0.016 units/mL, while isolate G20 from Ranu Grati was the lowest, reaching about 0.0001 Unit/mL. Based on the morphological and molecular identification, the KN bacterial isolate is classified as the Bacillus cereus group with 99.4-100% sequence similarity, closely related to Bacillus paramycoides (NR 157734.1).

scholarly journals 307 Characterization of Amylolytic Activities of Tulip Bulb Scales

HortScience ◽  
1999 ◽  
Vol 34 (3) ◽  
pp. 495D-495
Author(s):  
Anil P. Ranwala ◽  
William B. Miller
Keyword(s):  
Enzyme Activity ◽  
Amylase Activity ◽  
Gel Filtration ◽  
Soluble Starch ◽  
Degree Of Polymerization ◽  
Exchange Chromatography ◽  
Product Analysis ◽  
Ph Optimum ◽  
Tulip Bulb ◽  
Tulipa Gesneriana

Amylolytic activities extracted from scales of tulip (Tulipa gesneriana L. cv. Apeldoorn) bulbs stored at 4 °C for 6 weeks under moist conditions were characterized. Anion exchange chromatography of enzyme extract on DEAE-Sephacel revealed three peaks of amylolytic activity. Three enzymes showed different electrophoretic mobilties on nondenaturing polyacrylamide gels. The most abundant amylase activity was purified extensively with phenyl-agarose chromatography, gel filtration on Sephacryl S-200, and chromatofocusing on polybuffer exchanger PBE 94. The purified amylase was determined to be an endoamylase based on substrate specificity and end product analysis. The enzyme had a pH optimum of 6.0 and a temperature optimum of 55 °C when soluble starch was used as the substrate. The apparent Km value for soluble starch was 1.28 mg/ml. The inclusion of 2 mM CaCl2 in the reaction mixture resulted in a 1.4-fold increase in the enzyme activity. The presence of calcium ions also enhanced the thermo-stability of the enzyme at higher temperatures. The enzyme was able to hydrolyze soluble starch, amylose, amylopectin, and beta-limit dextrin, but it had no activity against pullulan, inulin, maltose, or p-nitrophenyl alpha-glucopyranoside. Only maltooligosaccharides, having a degree of polymerization of 7 or more, were hydrolyzed to a significant extent by the enzyme. Exhaustive hydrolysis of soluble starch with the enzyme yielded a mixture of maltose and matlooligosaccharides. This amylase activity was not inhibited by alpha- or beta-cyclodextrin upto a concentration of 10 mM. Maltose at a 50 mM concentration partially inhibited the enzyme activity, whereas glucose had no effect at that concentration.

scholarly journals Factors responsible for production of amylases from Aspergillus fumigatus Fresenius

2013 ◽  
pp. 11-20 ◽  
Author(s):  
Munira Akhter ◽  
Md Towhid Hossain ◽  
MN Anwar
Keyword(s):  
Enzyme Activity ◽  
Aspergillus Fumigatus ◽  
Ammonium Nitrate ◽  
Amylase Activity ◽  
Soluble Starch ◽  
Culture Ph ◽  
Enzyme Substrate ◽  
Amylase Production ◽  
Initial Culture

Aspergillus fumigatus Fresenius showed maximum amylase production at 27°C with an initial culture pH of the medium 6.0 after 72 h. of incubation. One per cent soluble starch and 0.15% ammonium nitrate in the medium supported the highest amylase activity. During enzyme-substrate reaction maximum enzyme activity was observed at 50°C and pH 4.0 with 2% starch. DOI: http://dx.doi.org/10.3329/cujbs.v3i1.13402 The Chittagong Univ. J. B. Sci.,Vol. 3(1&2):11-20, 2008

scholarly journals Isolation and screening of resistant bacteria of heavy metal (Fe) at ship dismantling

2018 ◽  
Vol 197 ◽  
pp. 13020 ◽  
Author(s):  
Harmin Sulistiyaning Titah ◽  
Herman Pratikno ◽  
Atiek Moesriati ◽  
Rizky Islami Putera ◽  
Muhammad Fauzul Imron
Keyword(s):  
Sea Water ◽  
Agar Medium ◽  
Iron Concentration ◽  
Ferrous Metal ◽  
Resistant Bacteria ◽  
Qualitative And Quantitative Analysis ◽  
Clear Zone ◽  
Qualitative And Quantitative ◽  
Ferrous Metals ◽  
Nutrient Agar Medium

The activity of ship dismantling is one of the sources of metal pollutant that polluted the environment. The aims of this study were analyze the iron concentration from the ship dismantling area, to isolate the bacteria from those area, and to determine the bacteria resistant on iron. Samplings was conducted in three point sampling at sea water and soil coast, respectively. Isolation of bacteria was carried out using pour plate methods. All isolated bacteria in seawater and soil samples were inoculated on nutrient agar medium (NA) containing ferrous metals (Fe2+) with various concentrations (0; 1,000; 2,000; 3,000; 4,000; 5,000; and 6,000 μg/mL). Based on the results, sea water and soil indicated that those area have contaminated with iron. The concentration of iron in seawater was 1.03, 1.01 and 1.00 μg/mL, respectively. Meanwhile, the concentration of iron in soil was 962.0, 966.05, 981.00 mg/kg, respectively. The result of qualitative and quantitative analysis showed that the isolates of AT, AL and CL coded bacteria have high resistance to the effect of iron. It indicated with clear zone of 6.00-7.00 mm at 6,000 μg/mL. In conclusion, both of bacteria are potential to be used for bioremediation of the ferrous metal (Fe2+) in further investigation.

scholarly journals Amylolytic ability of bacteria isolated from termite (Coptotermes sp.) gut

2018 ◽  
Vol 23 (1) ◽  
pp. 14 ◽  
Author(s):  
Putri Dwi Mulyani ◽  
Radhiyah Mardhiyah Hamid ◽  
Rifqi Zahroh Janatunaim ◽  
Yekti Asih Purwestri
Keyword(s):  
Amylase Activity ◽  
Rrna Gene ◽  
Optimum Temperature ◽  
Bacterial Isolates ◽  
16S Rrna Gene Sequences ◽  
Clear Zone ◽  
Optimum Ph ◽  
Iodine Test ◽  
Brevibacillus Parabrevis

BSR 2, BSR 3, BSR 8, and BSR 9, different bacteria isolated from the termite gut, have been shown to possess cellulolytic activities, but their amylolytic ability has heretofore been unknown. This study attempted to fill in this knowledge gap. The formation of a clear zone using the iodine test showed that the bacteria were able to produce and secrete amylase. Based on the results, the best cultivation times for strains BSR 2, BSR 3, BSR 8, and BSR 9 were 6, 3, 2, and 2 d, respectively, yielding amylase activities of 2.59 ± 0.13 U/mg, 2.00 ± 0.08 U/mg, 1.67 ± 0.10 U/mg, and 1.55 ± 0.12 U/mg, respectively. BSR 2 had the highest amylase activity compared with the other bacterial isolates. The optimum ph for bacterial amylase activity of BSR 2 was 7.0, and the optimum temperature was 40°C. The molecular characterization of isolates BSR 2, BSR 3, BSR 8, and BSR 9 was based on 16S rRNA gene sequences. Isolates BSR 8 and BSR 9 were thus identified as Brevibacillus parabrevis and Brevibacillus sp. With similarities amounting to 92.48% and 95.91%, while the BSR 3 isolate was identified as Pseudomonas alcaligenes with a similarity of 94.29%, and the BSR 2 isolate could not be identified yet.

scholarly journals CORRELATION BETWEEN CLASSROOM POPULATION, VENTILATION BACTERIAL LOADS AND THIER ANTIMICROBIAL PATTERNS IN SCHOOLS WITHIN IKOT EKPENE, NIGERIA

2019 ◽  
Vol 2 (4) ◽  
pp. 86 ◽  
Author(s):  
Aniefon Alphonsus Ibuot ◽  
Iniobong Ime James ◽  
Nsikan Samuel Udoekong ◽  
Mayen Ben ◽  
Christiana Utibe Etuk ◽  
...  
Keyword(s):  
Indoor Air ◽  
Agar Medium ◽  
Health Condition ◽  
Plate Count ◽  
Bacterial Isolates ◽  
Ambient Conditions ◽  
Nutrient Agar Medium ◽  
Plate Counts ◽  
Aerobic Plate Count ◽  
Negative Relationships

Indoor air of classroom in eight schools (4 nursery; NS1, NS2, NS3 and NS4, and 4 secondaries; SS1, SS2, SS3 and SS4) within Ikot Ekpene, Akwa Ibom State, Nigeria, were analyzed at ambient and populated sampling conditions using natural sedimentation on nutrient agar medium. The results revealed varying ventilation patterns in each of the classrooms, and the following airborne bacterial counts; NS1 (16.6 cfu/m3), NS2 (13.3 cfu/m3), NS3 (23.3 cfu/m3), NS4 (33.3 cfu/m3), SS1 (6.6 cfu/m3), SS2 (6.6 cfu/m3), SS3(28.3 cfu/m3) and SS4 (15 cfu/m3) at ambient sampling and 40 cfu/m3, 41.6 cfu/m3, 58.3 cfu/m3, 68.3 cfu/m3, 6.6 cfu/m3, 31.6 cfu/m3, 56.6 cfu/m3 and 25 cfu/m3 respectively at populated sampling. Bacterial isolates identified were Lactobacillus, Staphylococcus, Bacillus, Rothia, Kurthia, Corynebacterium, Pseudomonas, Brevibacterium, and Flavobacterium. Statistical analysis of the results revealed negative relationships between class area and aerobic plate counts (p>0.05), class population and aerobic plate count (p>0.805), and significant increase in aerobic plate counts at populated conditions over that at ambient conditions (p<0.05). The results therefore point to the dimensions of classrooms, ventilation and population of the classrooms as important factors in determining the bacterial air quality, and invariably affecting the health condition of students.

Sign in / Sign up

Export Citation Format

Share Document