scholarly journals Screening of Extracellular Keratinase Producing Bacteria from Feather Processing Areas in Vellore, Tamil Nadu, India

2010 ◽  
Vol 2 (3) ◽  
pp. 559 ◽  
Author(s):  
H. Prasad V ◽  
G. Kumar ◽  
Kartik L ◽  
B. Rao KV
Keyword(s):  
Enzyme Activity ◽  
Tamil Nadu ◽  
Agar Medium ◽  
Agar Plate ◽  
Soil Samples ◽  
Bacillus Sp ◽  
Bacterial Isolates ◽  
Plate Method ◽  
Keratinolytic Activity ◽  
Plating Method

The aim of the current study was to isolate keratinolytic bacteria from the soil samples collected from different feather processing areas in Vellore, TN, India. The isolation was performed by serial dilution and plating method. Total eight bacteria were isolated from the collected soil samples. All isolates were screened for keratinolytic activity by Casein agar plate method, among eight bacterial isolates only one (H5) isolate showed the keratinolytic activity in Casein agar medium. H5 isolate (potential strain) was identified as Bacillus sp. by microscopic and biochemical experiments. The best enzyme activity was observed at pH 7 and temperature 30ºC.  Keywords: Keratinolytic; Spread plate method; Bacillus sp.; Bacteria. © 2010 JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights reserved. DOI: 10.3329/jsr.v2i3.4567              J. Sci. Res. 2 (3), 559-565 (2010) 

scholarly journals Aktivitas Enzim Amilase Isolat Bakteri Amilolitik dari Tepung Sagu Basah dan Lingkungan Tempat Penyediaannya Secara Tradisional di Jayapura

2018 ◽  
Vol 6 (2) ◽  
pp. 47-52
Author(s):  
Suprapto Surapto ◽  
Tri Gunaedi ◽  
Basa T. Rumahorbo
Keyword(s):  
Enzyme Activity ◽  
Agar Medium ◽  
Amylase Activity ◽  
Soluble Starch ◽  
Bacterial Isolates ◽  
Clear Zone ◽  
Plate Method ◽  
Crude Enzyme Extract ◽  
Nutrient Agar Medium ◽  
Bacillus Subtilis Atcc

The study about the activity of the enzyme amylase from amylolytic bacterial isolates from wet sagoo starch and  its traditional provision environment had been done in Jayapura. The purposes of this study were to determine the activity of amylase enzyme and to identify the bacteria isolated from wet sagoo starch and its processing environment in Jayapura district. The method used was an experimental laboratorium in which isolation of amylolytic bacteria was performed by using nutrient agar medium with 1% soluble starch on spreed pour plate method. The enzyme activity was detected with 0.2% iodine in 2% potassium iodide which were able to form a clear zone. The protein content of the crude enzyme extract was determined by the Bradford method using bovine serum albumin (BSA). Amylase enzyme activity was determined by the formula: DUN/ml = [(R0-R1)/R0] [dilution factor] DUN/ml (dextrinizing units per ml). The results showed that there were 15 isolates amylolytic bacteria. Four (4) bacterial isolates have amylolytic power of more than 30 mm. The amilase activity of amylolytic bacterial of all  isolates were quite high: which were 35 577, 18 903,  32 106 and 46 600 U/mg for SU4, SU13, SU23 and SU40 respectively. The identification of isolates indicated that the three isolates are members of the Bacillus cereus ATCC 14 579 types with a similarity value of 71.70% to 81.10%, and one isolate is Bacillus subtilis ATCC 6501 members with a similarity value of 94.30%. Keywords: Amylolytic bacteria, amylase activity, characterization, sago flour.

scholarly journals Studies on decolorization of textile dye by using Pseudomonas and bacillus sp from the contaminated effluent soil samples of Kovilpatti, Thoothukudi district of Tamil Nadu

2019 ◽  
Vol 11 (1) ◽  
pp. 134-137 ◽  
Author(s):  
Kannan D ◽  
Renuga Devi ◽  
A. G. Murugesan ◽  
S. Rajan
Keyword(s):  
Contaminated Soil ◽  
Tamil Nadu ◽  
Soil Samples ◽  
Toxic Chemicals ◽  
Textile Dye ◽  
Pseudomonas Sp ◽  
Bacillus Sp ◽  
Bacterial Strains ◽  
Textile Industries ◽  
Source Of Pollution

Textile industries releasing large amount of effluent which contains textile dyes and toxic chemicals and it is one of the major source of pollution also contaminating water bodies. To remove that, bacteria have been of great attention because of their ability to treat effluent. The present study was undertaken to exploit the ability of Pseudomonassp and Bacillus sp from dye contaminated soil samples for bioremediation for dye effluent. Among the bacterial strains used in the study. Pseudomonas sp emerged out to be most potent decolorizer in comparison to Bacillus sp with the degree of decolorization of 90.0 %. Thus, it was concluded that the Pseudomonas sp had highest color removing capacity from contaminated effluent soil samples. 

scholarly journals Bio-remedial Effect of Specific Micro-organisms (Bacteria) on Used Engine Oil: A Case Study of Some Mechanic Workshops in Maiduguri Metropolitan Council (MMC)

2020 ◽  
Vol 1 (3) ◽  
Author(s):  
Ibrahim Yerima ◽  
G. A. Felix ◽  
M. I. Ahmad
Keyword(s):  
Zea Mays ◽  
Agar Medium ◽  
Bacterial Species ◽  
Soil Samples ◽  
Engine Oil ◽  
Bacillus Sp ◽  
Used Engine Oil ◽  
Micro Organisms ◽  
Effective Growth

The potential of three micro-organisms (Pseudomonas, Streptococcus and Bacillus sp) were isolated from hydrocarbon contaminated soil and were evaluated for their biodegradation ability. The rate of biodegradation of the engine oil in the soil samples were exposed to used engine oil with different exposure rates of 5,10,15 and 20 years  were studied for a period of three (3) weeks under greenhouse experiment. The soil samples were obtained from four different mechanic workshops in M.M.C and they were plated on nutrients agar and oil agar medium to isolate the bacterial species from the spilled soil samples. All the micro-organisms used in this study showed their abilities to remediate soil exposed to used engine oil and the remediated soil samples were able to support the growth of Maize ( Zea mays) after 10 years  effective growth

scholarly journals Enrichment and Biopelletization of Phosphate Solubilizing Diazotrophic Bacterial Isolates Using Earthworm as a Tool

2019 ◽  
pp. 1-5
Author(s):  
M. Chandana ◽  
G. V. Venkataramana
Keyword(s):  
Finger Millet ◽  
Agar Medium ◽  
Cow Dung ◽  
Initial Population ◽  
Bacillus Sp ◽  
Plate Method ◽  
Hind Gut ◽  
Phosphate Solubilizing ◽  
Dilution Plate Method

Aims: The beneficial role of earthworm Eudrilus eugeniae  in enhancing the populations of two phosphate solubilizing diazotrophic isolates viz., Bacillus sp. (DT) and Azotobacter chroococcum (DT),  isolated from the rhizosphere of finger millet [Eleusine coracana (L) Gaertn] was studied. Place and Duration of Study: The experiments were conducted at the campus of M/s. Chaitra Biofertilizers and Chemicals (P) Ltd, Mysore, between January – June 2018. Methodology: To the glass jars containing three week old partially decomposed green leafy material and cow dung, 0.5 gram of lignite based phosphate solubilizing diazotrophic isolates were added.  To this eight medium sized earthworms were allowed and moisture is maintained at 50-60%. The population of isolates in the gut  of earthworm and vermicasts were estimated on 2nd, 20th, 40th and 60th day by dilution plate method using Pikovskaya’s  and Jensen’s agar medium Results: The population of Bacillus sp. (DT) and A. chroococcum (DT) increased by 22.14, 42.14 and 97.62 percent in the fore gut, mid gut and hind gut regions respectively while A. chroococcum (DT) by 24.05, 41.19 and 110.95 percent as compared to their initial population in the feeding material.  Both the isolates increased enormously in the vermicasts up to 40th day and thereafter declined as recorded in 60 days old vermicasts.  Bacillus sp. (DT) increased by 196.91 and 247.16 percent in 20 and 40 day old vermicasts and decreased by 54.72 percent on 60th day while A. chroococcum (DT) recorded an increase of 217.34 and 270.77 percent and thereafter declined by 41.18 percent in 60 days old casts. Conclusion: The earthworm can be used as a tool for secondary level multiplication and biopelletization of the isolates to produce enriched vermicompost for use in finger millet cultivation.  It also indicated that the vermicasts should be applied soon after it is harvested.

scholarly journals SCREENING OF MULTI BIOACTIVE METABOLITES PRODUCING MICROORGANISMFROM GARDEN SOIL OF DR. M. G. R EDUCATIONAL AND RESEARCH INSTITUTE, CHENNAI

2020 ◽  
pp. 87-90
Author(s):  
SENTHILRAJR ◽  
GEJALAKSHMI S. ◽  
THARUNKUMAR M. ◽  
SANTHOSH A. ◽  
LOKESH V. ◽  
...  
Keyword(s):  
Agar Medium ◽  
Hydrolytic Enzymes ◽  
Soil Samples ◽  
Garden Soil ◽  
Bioactive Metabolites ◽  
Bacillus Species ◽  
Plate Method ◽  
Effective Screening ◽  
Starch Agar ◽  
Research Institute

Objective: Screening of industrial important bioactive metabolites of Antibiotics, hydrolytic enzymes producing microorganism from garden soil of Dr. M. G. R Educational and Research Institute, Chennai. Methods: Desired ten soil samples taken and were serially diluted. Crowded plate method used for antibiotic-producing microorganism and Starch agar medium and gelatin medium tests were performed for hydrolytic enzymes (Amylase and Gelatinase). Results: Among the tested soil samples, antibiotic producing microorganisms were not found, but has hydrolytic enzymes amylase and gelatinase. Thus screened soil samples biochemically identified as Bacillus species. Conclusion: This study concludes that, the collected sample, produced Antibiotic negative result and it’s possess other industrial important hydrolytic enzymes. Thus Screening of more bioactive metabolites producing ability from a single isolate, will be more useful for effective screening.

scholarly journals Bacteria Associated with Petroleum Hydrocarbon Contaminated Soil from NNPC Depot

2020 ◽  
pp. 77-82
Author(s):  
S. O. Bankole ◽  
M. B. Oyedeji ◽  
F. O. Akanni ◽  
E. P. Chukwudebe ◽  
A. O. Olatunji ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Local Government ◽  
Contaminated Soil ◽  
Petroleum Hydrocarbon ◽  
Research Work ◽  
Soil Samples ◽  
Pseudomonas Sp ◽  
Bacillus Sp ◽  
Plate Method ◽  
Pseudomonas Species

The microorganisms associated with soil polluted with petroleum hydrocarbon were isolated at NNPC depot Ibadan, in Ido Local Government Areas of Oyo State. Soil samples were taken from three different points along the point of discharge. The spread plate method was used to isolate the microorganisms found in these rivers and were later identified. From the research, seven different species of microorganism were isolated, which are Pseudomonas sp, Bacillus sp., Micrococcus sp. Staphylococcus aureaus sp., Enterobacter sp., Klebsiella sp. and Escherichia coli. Pseudomonas species were found to be more prominent in the samples collected in a research work. This suggested that the isolates are resistant to the petroleum hydrocarbon and can be recommended as candidates for the clean-up of petroleum hydrocarbon contaminated soil.

Isolation and Identification of Cellulase Producing and Sugar Fermenting Bacteria for Second-Generation Bioethanol Production

2021 ◽  
Vol 10 (4) ◽  
pp. 699-711
Author(s):  
Weerasinghe Mudiyanselage Lakshika Iroshani Weerasinghe ◽  
Dampe Acharige Tharindu Madusanka ◽  
Pathmalal Marakkale Manage
Keyword(s):  
Enzyme Activity ◽  
Alternative Fuels ◽  
Cellulase Activity ◽  
Crude Enzyme ◽  
Bioethanol Production ◽  
Bacillus Sp ◽  
Bacterial Isolates ◽  
Cellulase Enzyme ◽  
Fermenting Bacteria ◽  
Achromobacter Sp

Over the last decades, the negative impacts of fossil fuel on the environment and increasing demand for energy due to the unavoidable depletion of fossil fuels, has transformed the world’s interests towards alternative fuels. In particular, bioethanol production from cellulosic biomass for the transportation sector has been incrementing since the last decade. The bacterial pathway for bioethanol production is a relatively novel concept and the present study focused on the isolation of potential “cellulase-producing” bacteria from cow dung, compost soil, and termite gut and isolating sugar fermenting bacteria from palm wine. To select potential candidates for cellulase enzyme production, primary and secondary assays were conducted using the Gram’s iodine stain in Carboxy Methyl Cellulose (CMC) medium and the Dinitrosalicylic acid (DNS) assays, respectively. Durham tube assay and Solid-Phase Micro-Extraction (SPME) coupled with Gas Chromatography-Mass Spectrometry (GC-MS) was used to evaluate the sugar fermenting efficiency of the isolated bacteria. Out of 48 bacterial isolates, 27 showed cellulase activity where Nocardiopsis sp. (S-6) demonstrated the highest extracellular crude enzyme activity of endoglucanase (1.56±0.021 U) and total cellulase activity (0.93±0.012 U). The second-highest extracellular crude enzyme activity of endoglucanase (0.21±0.021 U) and total cellulase activity (0.35±0.021 U) was recorded by Bacillus sp. (T-4). Out of a total of 8 bacterial isolates, Achromobacter sp. (PW-7) was positive for sugar fermentation resulting in 3.07% of ethanol in broth medium at 48 h incubation. The results of the study revealed that Nocardiopsis sp. (S-6) had the highest cellulase enzyme activity. However, the highest ethanol percentage was achieved with by having both Bacillus sp. (T-4) and Achromobacter sp. (PW-7) for the simultaneous saccharification and fermentation (SSF) method, as compared to separate hydrolysis and fermentation (SHF) methodologies. 

KEBERADAAN BAKTERI PELARUT FOSFAT DAN AKTIVITAS ENZIM FOSFATASE TANAH DAERAH PERAKARAN TANAMAN OBAT DARI KEBUN RAYA CIBODAS

2018 ◽  
Vol 6 (3) ◽  
Author(s):  
Suliasih Suliasih
Keyword(s):  
Alkaline Phosphatase ◽  
Soil Sample ◽  
Rhizosphere Soil ◽  
Botanical Garden ◽  
Soil Enzyme ◽  
Soil Samples ◽  
Phosphate Solubilizing Bacteria ◽  
Bacillus Sp ◽  
Phosphate Solubilizing ◽  
Serratia Rubidaea

A study was undertaken to investigate to occurance of phosphate solubilizing bacteria from rhizosphere soil samples of medicine plants in Cibodas Botanical Garden. 13 soil samples of medicine plants are collected randomly The result shows that 71 isolates of phosphate solubilizing bacteria were isolated, and 10 species of these organism was identified as Azotobacter sp, Bacillus sp, Chromobacterium sp, C.violaceum, Citrobacter sp. , Enterobacter sp., E. liquefaciens. Nitrosomonas sp., Serratia rubidaea, Sphaerotillus natans. Azotobacter sp. And Bacillus sp. Are found in all of soil tested. Conversely, Serratia rubidaea is only in the sample from rhizosphere of Plantago mayor The activity of acid alkaline phosphatase in soil tested ranged from 0.78 – 60,18 ugp nitrophenole/g/h, with the higest values being recorded in soil sample from rhizosphere of “Lavender”.Keywords : phosphate solubilizing bacteria, soil enzyme phosphatase

scholarly journals Screening and Characterization of Potent Poly Glutamic Acid Producing Bacillus sp. Isolated from Kinema, Water and Soil Samples

Heliyon ◽  
2021 ◽  
pp. e07715
Author(s):  
Punam Thapa ◽  
Alina Thapa ◽  
Sujan Khadka ◽  
Sanjeep Sapkota ◽  
Om Prakash Panta ◽  
...  
Keyword(s):  
Glutamic Acid ◽  
Soil Samples ◽  
Bacillus Sp ◽  
Water And Soil Samples
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