Sensitivitas Antibiotik Terhadap Bakteri yang Diisolasi dari Ulkus Diabetika di RSUD Abepura, Kota Jayapura

Diabetic ulcer is a complication of Diabetes mellitus that is often found on the legs and easily develop into an infection. For the prevention of this symtomps, antibiotic is required to accelerate the wound healing process. The purpose of this study was to determine the bacteria that infect diabetic ulcers and antibiotic sensitivity test against the bacterial isolates in Abepura hospitals, Jayapura. This study used exploratory laboratory and was conducted at the Regional Health Laboratory (Labkesda) in Jayapura. The results of the isolation and identification showed that 6 species of bacteria have been found. The bacteria which most infected patients with diabetic ulcers in hospitals Abepura was Escherichia coli (38%). The result of antibiotic sensitivity test in hospitals Abepura ceftazidime therapy, showed that antibiotic sensitivity of Siprofloxacin therapy was only 16.7%. Imipenem choice of antibiotics had a sensitivity of 100% againts 6 types of bacteria that cause infections in patients with diabetic ulcers. Other choices of antibiotics which had a sensitivity of over 50% is Nitrofurantoin (83.3%), Gentamicin, Kanamycin and Polymyxin B and 66.7% respectively.Key words: Bacterial infections, diabetic ulcers, antibiotic sensitivity

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Prevalence and antibiotic resistance profile of Listeria spp. associated with seafoods from fish catchment areas in Kerala, India

Veterinary World ◽

10.14202/vetworld.2021.777-783 ◽

2021 ◽

Vol 14 (3) ◽

pp. 777-783

Author(s):

K. Vrinda Menon ◽

B. Sunil ◽

C. Latha

Keyword(s):

Antibiotic Resistance ◽

Antibiotic Sensitivity ◽

Sensitivity Test ◽

Isolation And Identification ◽

Resistance Profile ◽

Fresh Fish ◽

Antibiotic Resistance Profile ◽

Catchment Areas ◽

Antibiotic Sensitivity Test ◽

Serotype 4B

Background and Aim: Listeria monocytogenes is a ubiquitous, intracellular pathogen which has been implicated as a cause of several foodborne outbreaks. This study aimed to generate information on the prevalence and antibiotic resistance profile of Listeria species isolated from seafood. Materials and Methods: A total of 400 samples of fresh fish, 100 samples of dry fish and 200 samples each of crustaceans and mollusks were collected from the fish catchment areas. All the samples were subjected to isolation and identification of Listeria spp. by two-step enrichment in UVM broth and plating on selective agar media (PALCAM) and then subjected to molecular characterization. L. monocytogenes isolates obtained during the study were subjected to serotyping by multiplex polymerase chain reaction. The isolates were also subjected to antibiotic sensitivity test. Results: The prevalence of L. monocytogenes in seafoods in the present study was 0.55%. The isolates of L. monocytogenes were found to possess all virulence genes, namely, iap, hlyA, actA, prfA, plcA, and inlA. All the isolates belonged to serotype 4b. The occurrence of Listeria innocua was found to be more and was detected in 16.77% of seafoods samples. Antibiotic sensitivity test revealed that all isolates were resistant to cefixime but were sensitive to almost all other commonly used antibiotics. Conclusion: The presence of Listeria spp. in raw seafood samples augments the need for implementation of good hygienic practices during the handling and processing of seafoods to safeguard the health of the consumers.

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A RETROSPECTIVE STUDY ON ANTIBIOTIC USAGE IN A TERTIARY CARE HOSPITAL

International Journal of Medical and Biomedical Studies ◽

10.32553/ijmbs.v4i6.1218 ◽

2020 ◽

Vol 4 (6) ◽

Author(s):

Anna Joy ◽

Aparna Anand ◽

Arathy R Nath ◽

Meera S Nair ◽

Dr. K. G. Prasanth

Keyword(s):

Retrospective Study ◽

Tertiary Care ◽

Antibiotic Sensitivity ◽

Sensitivity Test ◽

Actual Process ◽

Care Hospital ◽

Rational Use ◽

Antibiotic Susceptibility Test ◽

Antibiotic Sensitivity Test ◽

Rational Use Of Antibiotics

Antibiotics are one of the most commonly prescribed drugs today. Rational use of antibiotics is therefore extremely important as their injudicious use can adversely affect the patient. Drug Utilization Evaluation (DUE) is a system of ongoing systematic criteria based evaluation of drug that will help to ensure that medicines are used appropriately. It is drug/disease specific and can be structured so that it will assess the actual process of prescribing, dispensing, or administration of drug. The retrospective study was conducted At Pk Das Institute of Medical Sciences, Palakkad, Kerala for a duration of 6 months (February 2017 - January 2018). A source of data includes Patient case sheets &medication charts, nursing charts, culture & sensitivity reports. The inclusion criterion includes Patients aged between 18- 80 year, prescribed with oral and parenteral antibiotics. . On analyzing the gender, male gender (n= 111, 55.5%) were higher in numbers as compared to female counterparts (n=89, 44.5%). In our study the majority of the patients prescribed with antibiotics were with the clinical assessment of COPD (n=39, 19.5%), UTI (n=37, 18.5%) and LRTI (n=28, 14%), Bronchial asthma (n=19, 9.5%) respectively. On analyzing the data based on antibiotic sensitivity test, antibiotic test were performed and followed in (n=64,32%)prescriptions and in (n=47,23.5%)prescriptions were test is not followed respectively. In (n=89,44.5%) prescriptions, antibiotic sensitivity test is not performed. On analysis of antibiotics prescribed, the most commonly prescribed antibiotics were cephalosporins, of these ceftriaxone was highly prescribed of all (n=95). The high percentage of antibiotic prescriptions may indicate a high probability of irrational use. This study also point out irrational use of antibiotics are more leading to resistance, misuse and serious problems. So certain strategies should be put forward to strengthen rational use of antibiotics. Keywords: Antibiotics, Antibiotic Susceptibility Test, Irrational use, Resistance

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5026638 Antibiotic sensitivity test for pathogenic organisms present in mastitic milk

Biotechnology Advances ◽

10.1016/0734-9750(91)91224-o ◽

1991 ◽

Vol 9 (3) ◽

pp. 521

Keyword(s):

Antibiotic Sensitivity ◽

Sensitivity Test ◽

Antibiotic Sensitivity Test ◽

Pathogenic Organisms ◽

Mastitic Milk

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BACTERIOLOGICAL EXAMINATION OF PUS SAMPLE COLLECTED FROM A COW: A CASE STUDY

International Journal of Research -GRANTHAALAYAH ◽

10.29121/granthaalayah.v4.i12.2016.2389 ◽

2016 ◽

Vol 4 (12) ◽

pp. 31-33

Author(s):

Subha Ganguly

Keyword(s):

Present Article ◽

Antibiotic Sensitivity ◽

Neck Region ◽

Sensitivity Test ◽

Bacteriological Examination ◽

Antibiotic Sensitivity Test

The present article reports on the bacteriological examination and antibiotic sensitivity test of pus sample collected from an abscess at the neck region of a cow.

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Analysis of River Water Along Klang Valley to Evaluate the Prevalence of Antibiotics Resistant Strains within Urbanized Areas of Selangor, Malaysia

Proceedings International ◽

10.33263/proceedings21.059059 ◽

2020 ◽

Vol 2 (1) ◽

pp. 59

Keyword(s):

River Water ◽

Antibiotic Sensitivity ◽

Most Probable Number ◽

Resistant Bacteria ◽

Minimal Bactericidal Concentration ◽

Isolation And Identification ◽

Antibiotic Resistant ◽

Probable Number ◽

Antibiotic Sensitivity Test ◽

Klang Valley

The determination of antibiotic-resistant bacteria in Klang river water in Klang valley is performed as the river exposed to various environments. The analysis is performed through enumeration, isolation, and identification process. The water samples were obtained from the origin of the river, housing region, and hospital region. The coliforms obtained through enumeration and identification was then used to determine antibiotic sensitivity, minimum inhibitory concentration (MIC), and minimal bactericidal concentration (MBC). The level of coliforms was indicated through the most probable number (MPN), which 70 MPN per 100 ml of river water in the origin of the river while housing and hospital regions showed more than 1600 MPN per 100 ml of river water. The results obtained from the antibiotic sensitivity test showed that the degree of resistance of coliforms is varied in different regions. The zone of inhibition to ampicillin and tetracyclin for coliforms in housing regions is 20 mm, while the coliforms in the hospital region are 6 mm and 7 mm, respectively. The overall results showed that the level of coliforms and the antibiotic sensitivity of coliforms are different in various regions. The coliforms in the hospital region are more resistant to antibiotics compared to the housing region.

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Study of ELISA and antibiotic sensitivity test for Salmonella enteritidis as experimental infection in mice

Baghdad Science Journal ◽

10.21123/bsj.6.1.114-122 ◽

2009 ◽

Vol 6 (1) ◽

pp. 114-122

Author(s):

Baghdad Science Journal

Keyword(s):

Salmonella Enteritidis ◽

Antibiotic Sensitivity ◽

Immunological Response ◽

Elisa Test ◽

Sensitivity Test ◽

Test Results ◽

Antibiotic Sensitivity Test ◽

Bacterial Sensitivity ◽

Antibiotics Sensitivity ◽

Tract Infections

Salmonella enteritidis one of more important as epidemiological bacteria between other salmonella types. It is very important pathologically that cause food poising and gastrointestinal tract infections. This study includes some of immunological changes that appear by ELISA test and antibiotic sensitivity test against these bacteria in mice. ELISA test results appears high immunological response happen after 3 days of inoculation, mean titration readings beginning 0.198 and the maximum mean titration after 15 days of inoculation 1.538 and begin to decrease after this time slowly to remain about 0.297 after 40 days of inoculation. An antibiotics sensitivity test result appears, this bacteria sensitive to Chloramphenicol, Ceftriaxone, Ciprofloxacin and Cotrimaxazol. Resistance to Neomycin, Streptomycin and Rifampicin, while intermediate against Ampicilin and Amoxicillin. Another test we use Vitek system to know bacterial sensitivity against to more another types of antibiotics and to confirm between some of them.

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Antibiotic Sensitivity Test

Clinical Microbiology and Parasitology (For DMLT Students) ◽

10.5005/jp/books/12721_27 ◽

2016 ◽

pp. 150-150

Author(s):

Nanda Maheshwari

Keyword(s):

Antibiotic Sensitivity ◽

Sensitivity Test ◽

Antibiotic Sensitivity Test

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Biofilm Formation by Uropathogens and Their Susceptibility Towards Antimicrobial Therapy

Medical Journal of Shree Birendra Hospital ◽

10.3126/mjsbh.v18i1.20189 ◽

2019 ◽

Vol 18 (1) ◽

pp. 13-22 ◽

Cited By ~ 1

Author(s):

Mahesh Prakash Bhatta ◽

Asmita Sapkota ◽

Pushpa Subedi ◽

Sunita Baniya Chhetri ◽

Dhaka Raj Pant ◽

...

Keyword(s):

Antimicrobial Therapy ◽

Biofilm Formation ◽

Pathogenic Bacteria ◽

Antibiotic Sensitivity ◽

Isolation And Identification ◽

Plate Method ◽

Exopolymeric Substances ◽

Antibiotic Sensitivity Test ◽

Health Care Associated Infection ◽

Klebsiella Spp

Introduction: Urinary tract infection (UTI) is the most common health care associated infection caused by various pathogenic bacteria. Biofilms are communities of bacteria that are held together by exopolymeric substances that protect against the antimicrobial therapy and other environmental assaults. The aim of this study was to estimate the prevalence of biofilm forming bacteria in Nepalese population and to study the emergence of antimicrobial resistance among biofilm producing bacteria in comparison to non-biofilm producing bacteria. Methods: A total of 785 clean-caught-mid-stream urine samples were collected. After isolation and identification of uropathogens, they were further processed for detection of biofilm formation by two methods (Congo Red Agar method and Tissue Culture Plate method) as well as for antibiotic sensitivity test. Results: Out of total collected samples, 12.74% were found to be associated with UTI, among them 67% were Escherichia coli, 10% were Klebsiella spp, 7% were Pseudomonas spp, 6% were Staphyloccous aureus, 4% were Enterobacter spp, 3% were Proteus spp, 2% were Citrobacter spp and remaining 1% was Staphylococcus saprophyticus. Among isolated organisms, the ratio of bioflim positive organism to bioflim negative organism was found to be 9:11. Nitrofurantoin, Tobramycin, Chloramphenicol, Amikacin and Imipenem were found to be significantly more sensitive in biofilm negative bacteria as compared to biofilm positive bacteria with p values of 0.000, 0.001, 0.000, 0.000 and 0.001. Conclusions: The prevalence rate of multidrug resistance in bacterial uropathogens was higher in biofilm producers as compared to non-biofilm producers. Biofilm forming characteristic of bacteria make them more resistant to antibiotics.

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